• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2004년도 International Conference of Sericultural and Entomological Sciences
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• pp.58-59
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• 2004

• 생명과학회지
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• 제17권8호통권88호
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• pp.1129-1134
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• 2007

bPDI가 ER내 misfolding 단백질의 생성을 제한함으로써 곤충변역과 관계하는지를 해석하기 위하여 bPDI가 과발현(overexpression)되는 곤충세포주와 이와 반대로 bPDI가 억제발현(knock-down)되는 곤충세포주를 제작하여 bPDI가 곤충면역에 관련하는지를 해석하였다. bPDI가 과발현되는 세포주 (Sf9-bPDI)는 정상세포주(Sf9)나 pIZT/V5-His 벡터만 도입된 세포주(Sf9-pIZT)에 비하여 생존율이 30% 이상 높았지만, bPDI의 전사체 발현이 억제된 세포주(Sf9-bPDI-dsRNA)는 오히려 정상세포주나 pIZT/V5-His 벡터만 도입된 세포주에 비하여 생존율이 약 15%낮았다. 이와 같은 결과로써, bPDI는 ER내 misfolding 단백질의 생성을 제한함으로써 곤충의 ERSE과 밀접하게 관련할 것이라 추정할 수 있었다.

• Radiation Oncology Journal
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• 제22권2호
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• pp.138-141
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• 2004

목적 : 본 연구진이 기왕에 입증한 바 있는 히스톤탈아세틸효소 억제제 trichostatin A (TSA)가 나타내는 방사선 감수성 증강 작용이 두경부암 세포주에서 발생하는 정도를 실험적으로 확인하고자 하였다. 대상 및 방법 : 인간 두경부암 세포주인 HN-3과 HN-9를 0, 50, 100, 200 nM의 TSA에 18시간 동안 전처치시킨 후 각각 0, 2, 4, 6, 8 Gy 방사선을 조사하였다. 세포생존곡선은 clonogenic assay를 이용하여 산출하였고 linear quadratic 모델에 따라 분석하였다 결과 : 방사선 조사 전 TSA처리는 HN-3과 HN-9 세포주의 방사선 감수성을 증강시켰다. 50 nM의 TSA로 처리된 HN-9 세포주에서 2 Gy 조사후 생존분획(SF2)은 유의한 수준으로 감소하였으나, HN-3 세포주는 200 nM의 TSA 처리 후 SF2가 유의하게 감소하였다. HN-3과 HN-9 세포주에서 200 nM TSA의 sensitizer enhancement ratio는 각각 1.84와 7.24였다. 결론 : 방사선 조사 전 히스톤탈아세틸효소 억제는 인간 두경부암 세포주의 방사선 감수성을 증가시켰으며, 이 증강 작용이 암세포주에서의 일반적으로 관찰되는 현상일 가능성이 크다고 제안한다.

• 대한한방내과학회지
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• 제29권3호
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• pp.543-553
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• 2008

Objective: The goal of this study was to determine the anti-asthma mechanism of SF on TNF-${\alpha}$ induced activation on A549 (human type II-like epithelial) cells. Using oligonucleotide microarray, we sought to establish the molecular mechanism of the protective effects of SF on A549 cells. Material & Methods : Cells were cultured in three different conditions: 1) negative control group was cultured in normal condition of DMEM, 2) positive control group was activated with TNF-${\alpha}$, IL-4. and IL-1${\beta}$, and 3) SF treated group was previously treated with 0.1${\mu}g/ml$ SF after TNF-${\alpha}$, IL-4. and IL-1 activation. Cells of positive control and SF treated groups were cultured for 30 min, 1hr, 3hr and 6hr. Results : The comparative analysis of the gene expression profile revealed that proinflammatory cytokines such as IL1F8, IL1F9, IL1R1. IL1RN, IL1RAPL1, IL8, TNFRSF4, TNFSF10c, TNFSF13, TRAF5, and TRAF7 and inflammation-related genes including MMP2, MMP11, MMP14, MMP15, MMP16, MMP19, MMP25, and MMP27 were down regulated with SF treatment. Cell adhesion molecule genes such as ITGB1, ITGBL1, selectin P ligand, selectin E, ICAM2, ICAM3, VCAM1, PECAM, FCER1G and MMP28 genes were also down-regulated in SF treated A549 cells. Conclusion : These results suggest that the anti-asthmatic effects of SF could be mediated by regulating specific genes related with cell adhesion, proinflammatory cytokine and inflammation-related genes in A549 cells.

• BMB Reports
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• 제32권5호
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• pp.429-435
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• 1999

Unlike the mammalian glucose transporter GLUT1, little is known about the nature of the endogenous sugar transporter(s) in insect cells. In order to establish the transport characteristics and other properties of the sugar transport proteins of Sf9 cells, a series of kinetic analyses was performed. A saturable transport system for hexose uptake has been revealed in the insect cells. The apparent affinity of this transport system(s) for 2-deoxy-D-glucose was relatively high, the $K_m$ for uptake being <0.5 mM. To further investigate the substrate and inhibitor recognition properties of the insect cell transporter, the ability of other sugars or drugs to inhibit 2-deoxy-D-glucose transport was examined by measuring inhibition constants ($K_j$). Transport was inhibited by D-mannose, D-glucose, and D-fructose. However, the apparent affinity of the C-4 epimer, D-galactose, for the Spodoptera transporter was relatively low, implying that the hydroxyl group at the C-4 position may play a role in the strong binding of glucose and mannose to the transporter. The results also showed that transport was stereoselective, being inhibited by D-glucose but not by L-glucose. It is therefore concluded that insect cells contain an endogenous glucose transport activity that in several aspects resembles the human erythrocyte glucose transporter. However, the mammalian and insect transporters were different in some of their kinetic properties, namely, their affinities for fructose and for cytochalasin B.

• 한국수정란이식학회지
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• 제33권1호
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• pp.1-11
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• 2018

Cellular cyclic adenosine-3' 5'-monophosphate (cAMP) modulator is known as meiotic inhibitor and can delays spontaneous maturation in IVM experiment. Among many cAMP modulators, the role of Pituitary adenylate cyclase activating polypeptide (PACAP) on IVM isn't known. The purpose of this study is to improve the maturation of oocytes derived from follicles ${\leq}3mm$ in diameter through PACAP as meiotic inhibitor during pre-in vitro maturation (pre-IVM). First, we checked PACAP and its receptors in cumulus cells and, to establish the optimal phase and concentration of PACAP for pre-IVM, we conducted chromatin configuration assessments. As a result, the rate of GV (Germinal Vesicle) according to duration of pre-IVM was significantly decreased 12 h and 18 h after IVM (87.1 and 84.1%, respectively) compared to 0 h (99.4%). When COC was cultured for 18 h, the GV rate in the $1{\mu}M$ of PACAP treatment group (82.1%) was significantly higher than any other PACAP treatment groups (60.5, 64.1, 74.4 and 69.9 %, respectively). So, we divided into four groups as follows; MF (the conventional IVM group, obtained from follicle from 3 to 6 mm in diameter), SF (the conventional IVM group, obtained from follicle ${\leq}3mm$ in diameter), Pre-SF(-)PACAP (IVM group including 18 h pre-IVM without $1{\mu}M$ of PACAP, obtained from follicle ${\leq}3mm$ in diameter) and Pre-SF(+)PACAP (IVM group including 18 h pre-IVM with $1{\mu}M$ of PACAP, obtained from follicle ${\leq}3mm$ in diameter). To examine the effect of PACAP during pre-IVM, we investigated analysis of nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels. In cumulus cells, PACAP receptors, ADCYAP1R1 and VIPR1 were detected but were not detected in oocytes. After IVM, the Pre-SF(+)PACAP had the highest Metaphase II rate (91.7%) among all groups (P<0.05). The GSH levels in the MF and Pre-SF(+)PACAP were significantly higher than in the other groups (P<0.05) and ROS levels was no significant difference among all groups. In conclusion, these results indicated that even though the oocytes were derived from SF, pre-IVM application of PACAP improved meiotic and cytoplasmic maturation by regulating intracellular oxidative stress.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.739-744
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• 2007

Insect cell lines and the control of infection for obtaining the maximum amount of polyhedrin-Cry1Ac-polyhedrin fusion protein from Bactrus in monolayer and suspension culture systems were tested. Growth rates of the Trichoplusia ni(High-Five) cell line in both culture systems were better than the other insect cell lines, Spodoptera frugiferda(Sf-9, Sf-21), Trichoplusia ni(Tn5), and Spodoptera exigua(Se301). The expression of the fusion protein in a monolayer culture showed that Se301 cells were 2.3-4.8 times more productive on a per cell basis than the other cell lines. However, in suspension culture, only High-Five cells were productive. High-Five cells infected with Bactrus at a multiplicity of infection(MOI) of 5 and a cell density of $3.0{\times}10^5$ cells per ml were more productive than the other infection condition in a suspension culture suitable for a large-scale production of baculovirus. In conclusion, for the large-scale production of Bactrus in vitro, High-Five cells showing good growth and high productivity are suitable.

• 한국산학기술학회논문지
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• 제15권9호
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• pp.5776-5782
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• 2014

음영 지역 해소 및 셀 커버리지 확대를 위하여 중계기를 활용하는 이동 통신 시스템이 널리 연구되고 있다. 본 논문에서는 중계기를 통해 다중 경로가 확보된 음영 지역에서 주파수 공간 블록 부호를 적용한 SC-FDMA(Single Carrier-Frequency Division Multiple Access) 전송 시스템을 제안하고, 중계기와 수신국 간의 신호 대 잡음 비를 기준으로 음영 지역을 위한 SC-FDMA 시스템의 성능을 비교한다. 중계기에서 복원된 전송 신호에 대하여 주파수 공간 블록 부호를 적용하여 재전송함으로서 중계기 기반 SC-FDMA 시스템의 수신 성능을 크게 향상시킬 수 있다. 본 논문에서 제안된 주파수 공간 블록 부호화 중계기 기반 SC-FDMA 시스템의 신호 대 잡음 비 성능이 단일 중계기 기반 SC-FDMA 시스템보다 $10^{-2}$ 심볼 오류율 수준에서 약 5 dB 개선됨을 실험을 통해 확인할 수 있다.

• BMB Reports
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• 제55권12호
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• pp.609-614
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• 2022

Mutation of the gene for adenomatous polyposis coli (APC), as seen in Apc^Min/+ mice, leads to intestinal adenomas and carcinomas via stabilization of β-catenin. Transmembrane 4 L six family member 5 (TM4SF5) is involved in the development of non-alcoholic fatty liver disease, fibrosis, and cancer. However, the functional linkage between TM4SF5 and APC or β-catenin has not been investigated for pathological outcomes. After interbreeding Apc^Min/+ with TM4SF5-overexpressing transgenic (Tg^TM4SF5) mice, we explored pathological outcomes in the intestines and livers of the offspring. The intestines of 26-week-old dual-transgenic mice (Apc^Min/+:Tg^TM4SF5) had intramucosal adenocarcinomas beyond the single-crypt adenomas in Apc^Min/+ mice. Additional TM4SF5 overexpression increased the stabilization of β-catenin via reduced glycogen synthase kinase 3β (GSK3β) phosphorylation on Ser9. Additionally, the livers of the dualtransgenic mice showed distinct sinusoidal dilatation and features of hepatic portal hypertension associated with fibrosis, more than did the relatively normal livers in Apc^Min/+ mice. Interestingly, TM4SF5 overexpression in the liver was positively linked to increased GSK3β phosphorylation (opposite to that seen in the colon), β-catenin level, and extracellular matrix (ECM) protein expression, indicating fibrotic phenotypes. Consistent with these results, 78-week-old Tg^TM4SF5 mice similarly had sinusoidal dilatation, immune cell infiltration, and fibrosis. Altogether, systemic overexpression of TM4SF5 aggravates pathological abnormalities in both the colon and the liver.

• 한국수소및신에너지학회논문집
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• 제27권4호
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• pp.365-371
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• 2016

Cell performance of the Zn-Br redox flow battery (ZBRFB) using two different type's membrane (Nafion117 and SF-600) was evaluated at $20mA/cm^2$ of current density in 1M (mol/L) $ZnBr_2$ + 2M KCl + 0.3M EMPBr(1-ethyl-1-methyl pyrrolidinium bromide) electrolyte. The average energy efficiencies of ZBRFB were 74.9% and 74.7% for Nafion117 and SF-600, respectively. The electrolyte added the 1-ethyl-3-methylimidazolium dicyanamide (EMICA) as an additive was tested for the electrolyte in ZBRFB using SF-600 at $30mA/cm^2$ of current density. An average energy efficiency of the ZBRFB was 74.5% and 77.4% for the electrolyte non-added EMICA and added 1wt% of EMICA, respectively. ZBRFB using the electrolyte added EMICA was showed the higher performance than that using the electrolyte non-added EMICA.