• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.4
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• pp.44-53
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• 2016

The era of Big Data has come and the importance of Big Data has been rapidly growing. The part of transportation, the Four-Step Travel Demand Model(FSTDM), a traditional Trip-Based Model(TBM) reaches its limit. In recent years, a traffic demand forecasting method using the Activity-Based Model(ABM) emerged as a new paradigm. Given that transportation means the spatial movement of people and goods in a certain period of time, transportation could be very closely associated with spatial data. So, I mined Spatial Big Data from SNS. After that, I analyzed the character of these data from SNS and test the reliability of the data through compared with the attributes of TBM. Finally, I built a database from SNS for the operation of ABM and manipulate an ABM simulator, then I consider the result. Through this research, I was successfully able to create a spatial database from SNS and I found possibilities to overcome technical limitations on using Spatial Big Data in the transportation planning process. Moreover, it was an opportunity to seek ways of further research development.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.

• The Journal of the Korea Contents Association
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• v.16 no.3
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• pp.33-42
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• 2016

Utilization of cultural contents is more and more increasing as it is believed to improve local problems and provide historical values. This kind of business is positive in terms of the fact that it attracts visitors into local areas and activate local commercial areas. Under this trend, the construction of specialized streets is required to be approached with first priority to solve problems of cities. So to speak, it's more important to solve intrinsic problems found in local communities and then approach cultural contents step-by-step rather than constructing new spaces with first priority. Moreover, cultural contents should accompany economic aspects which result in local community-friendly and sympathetically concentrated values. Under this sentiment, the efforts to suggest economic ripple effects for government local government's businesses using cultural contents are very important. This study analyzed how much economic ripple effects is yielded by reflecting cultural contents. As the result of analysis, specialized street with cultural contents applied are proved to be more concentrated on multiplier of expediting employment, income and tax and tourism industry than service industry. On the contrary to this, service industry showed more concentrated multiplier of expediting production, income, added values and chain reaction effect (coefficients for influence and sensitivity for front and rear side of tourism industry. Based on these results, construction of specialized streets with cultural contents applied is proved to help synergy effects for local economy.

• Korean Journal of Veterinary Service
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• v.35 no.1
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• pp.9-17
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• 2012

The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.

• Journal of Internet Computing and Services
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• v.20 no.6
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• pp.11-20
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• 2019

Numerous enterprises, organizations and individual users are exposed to large DDoS (Distributed Denial of Service) attacks. DDoS attacks are performed through a BotNet, which is composed of a number of computers infected with a malware, e.g., zombie PCs and a special computer that controls the zombie PCs within a hierarchical chain of a command system. In order to detect a malware, a malware detection software or a vaccine program must identify the malware signature through an in-depth analysis, and these signatures need to be updated in priori. This is time consuming and costly. In this paper, we propose a botnet detection scheme that does not require a periodic signature update using an artificial neural network model. The proposed scheme exploits Word2Vec and accelerated hierarchical density-based clustering. Botnet detection performance of the proposed method was evaluated using the CTU-13 dataset. The experimental result shows that the detection rate is 99.9%, which outperforms the conventional method.

• Clean Technology
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• v.17 no.4
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• pp.285-299
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• 2011

As one of the REACH obligations, the extended safety data sheet (eSDS) should be communicated within the supply chain under the REACH Regulation. Based on technical guidance documents published on the ECHAs website and survey of EU's recent REACH-related informations, this paper includes a study on details of how to develop exposure scenarios (ES) such as structure of ES, process of ES develpoment, standard workflows and key input data to develop ES with an introduction of eSDS concept. This paper also contains an overview on operational conditions (OCs) and risk management measures (RMMs) that are what to consider when building an ES. The structure of Chesar (Chemical Safety Assessment and Report tool) developed by European Chemicals Agency (ECHA) is studied with a review of the available exposure estimation tools for workers, environment and consumers. Case example of generic exposure scenario (GES) for organic solvent is presented. To guide Korean EU-exporting companies, their participating roles in three steps of preparing ES are addressed.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.417-428
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• 2011

Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated ($120^{\circ}C$ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.

• The KIPS Transactions:PartD
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• v.18D no.5
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• pp.371-384
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• 2011

This paper considers logistics decision support system which deals with transportation mode selection considering transportation and carbon emission cost. Transportation and carbon emission costs vary with the choice of transportation modes and to become competitive companies need to find proper transportation modes for their logistics services. However, due to the restricted capacity of transportation modes, it is difficult to balance transportation and carbon emission costs when designing logistics network including transportation mode choice for each service. Therefore this paper aims to analyze the trade-off relationship between transportation and carbon emission cost in mode selection of intermodal transportation and to provide optimal green logistics strategy. In this paper, the logistics decision support system is designed based on mixed integer programming model. To understand the trade-off relationship of transportation and carbon emission cost, the system is tested with various scenarios including transportation of containers between Seoul and Busan. The analysis results show that, even though sea transportation combined with trucking is competitive in carbon emission per unit distance travelled, the total cost of carbon emission and transportation for the sea transportation may not have competitive advantage over other transportation modes including rail and truck transportation modes. The sea-based intermodal logistics service may induce detours which have negative impacts on the overall carbon emission. The proposed logistics decision support system is expected to play key role in green logistics and supply chain management.

• Clean Technology
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• v.18 no.1
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• pp.1-13
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• 2012

Starting with textile products in the middle of 1990, environmental requirements on prohibition of hazardous substances in products have been led by EU member countries and expanded to electrical and electronic equipment with implementaion of RoHS (Restriction of the use of certain hazardous substances in electrical and electronic equipment) in 2006. Under EU REACH (Registration, Evaluation and Authorization of CHemicals), the concept of SVHC (Substances of Very High Concern) and resulting regulatory duties regarding it have been introduced to the supply chain of almost all industry sectors. In this technical review, kinds of hazardous substances, reasons for restrictions and related directives and regulations are reviewed with its influence on the international market. Suggestions are made how to cope with environmental regulations as well as mid-to-long term market strategy to secure global market competitiveness.

• Korean Journal of Veterinary Service
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• v.25 no.3
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• pp.245-257
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• 2002

The gene encoding the HN protein from the CBP-1 strain, a heat stable Newcastle disease virus (NDV) isolated from diseased pheasants in Korea, was characterized by reverse transcriptase- polymerase chain reaction(RT-PCR) and the nucleotide and amino acid sequences were analyzed following cloning of the HN gene. In all of the NDV strains studied, a 1.75 kb size cDNA fragment for the HN gene was generated by RT-PCR and smaller specific band sizes harboring the internal portions of the HN gene were also detected by using four pairs of primers. The RT-PCR was sensitive enough to detect viral transcripts when the virus titer was above 25 hemagglutination units. The amplified 1.75 kb cDNA was cloned into a BamHI site of the pVL1393 Baculo transfer vector. The nucleotide sequences of the 1,758 bp HN gene from the CBP-1 strain were determined by the dye terminator cyclic sequencing method. The gene sequences were compared among the strains of CBP-1, Texas GB, Beaudette C, LaSota, B1 and Ulster. The homology of the CBP-1 HN gene to other HN variants was 97.8% to Texas GB, 98.4% to Beaudette C, 95.4% to LaSota, 95.6% to B1 and 90.2% to Ulster. As the deduced 577 amino acid sequences were compared among the strains, the homology for CBP-1 HN appeared to be 96.7% to Texas GB, 97.9% to Beaudette C, 95.5% to LaSota, 95.5% to B1 and 92.7% to Ulster. It was evident that the amino acid sequences included 5 sites for N-asparagine linked glycosylation and 12 cysteine residues. The three conserved leucine residues within the predicted transmembrane domain of the HN protein are amino acid 30, 37 and 44. The three antigenic sites on the HN protein of NDV are amino acids 347(Glu), 481(Asn) and 495(Glu). These data indicate that the genotype of the CBP-1 strain is more closely associated with the strains of Texas GB and Beaudette C than it is for the LaSota, B1 and Ulster strains.