• Journal of Nutrition and Health
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• v.48 no.4
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• pp.327-334
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• 2015

Purpose: The objective of this study was to investigate the effects of (6)-gingerol, ginger components proliferation and adipocyte differentiation from early to lately steps. Methods: 3T3-L1 preadipocytes were cultured. Differentiation of confluent cells was induced with dexamethasone, isobutylxanthin and insulin for 2 day and cells were cultured by medium with insulin in presence of various concentrations 0, 25, 50, $100({\mu}mol/L)$ of (6)-gingerol for 4 day. Cell viability was measured using the EZ Cytox assay kit. In addition, we examined the expression of mRNA levels associated with each adipocyte differentiation step by real time reverse transcription polymerase chain reaction. Results: (6)-Gingerol inhibited adipocyte proliferation in a dose and time dependent manner. Expression of $C/EBP{\beta}$, associated with early differentiation step remained unchaged. However, intermmediate, late differentiation step and adipocytokines were effectively changed in dose-dependently manner in cell groups treated with (6)-gingerol. Conclusion: This study has shown that treatment with (6)-gingerol inhibited adipocyte proliferation as well as each adipocyte differentiation step. In particular, the (6)-gingerol more effectively inhibited adipocyte differentiation from intermmediate differentiation step.

• Journal of Korea Society of Industrial Information Systems
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• v.21 no.6
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• pp.109-123
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• 2016

This study investigates the importance of government's support for logistics security assurance through certification programs. First, the study analyzed priorities among the requirements of logistics firms through Analytic Hierarchy Process(AHP) and Quality Function Deployment(QFD) approaches. For this process we invited 21 logistics experts to assess the relationships between logistic firms' requirements and government policies regarding logistics security using the house of quality, a set of matrices of QFD. The results of this phase of the study revealed the priorities of logistics firms' goals regarding the diffusion of the government security certification program as follows: integrated logistics security systems(40.3%), strengthening government support systems(32.4%), and operational effectiveness of logistics security certification(27.2%). Second, a relative weights applied QFD method based on AHP was applied to determined the expected outcome of the logistics security certification program. The results indicated as follows: productivity improvement(28.4%), improved level of service(26.7%), logistics cost reduction(21.6%), advanced information systems(19.7%), and improved environmental protection(3.6%). The results of this study provide new insights concerning logistics firms' requirements for supply chain security and the importance of government's support policies through logistics security certification programs.

• Journal of Microbiology
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• v.44 no.1
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• pp.106-112
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• 2006

In this study, the genetic diversities of multi-resistant Salmonella typhimurium (ST) isolates were analyzed via the application of both pulsed field gel electrophoresis (PFGE) and Polymerase chain reaction (PCR) analysis methods, using 6 kinds of primers (REP, ERIC, SERE, BOX, P-1254 and OPB-17). And their discriminative abilities (DA) were also compared in order to determine the most effective and reliable analysis method. 118 S. typhimurium isolates, cultured from diverse animals and human patients in Korea beginning in 1993, were analyzed and subjected to a comparison of Simpson's index of diversity (SID), using both PFGE and PCR methods. PFGE by XbaI enzyme digestion allowed for discrimination into 9 pulsotypes, with high SID values (0.991) on the genomic DNA level. This shows that PFGE is a very discriminative genotypic tool, and also that multiple clones of S. typhimurium isolates had existed in domestic animals and humans in Korea since 1993. However, we could ultimately not to trace the definitive sources or animal reservoirs of specific S. typhimurium isolates examined in this study. Depending on the SID values, the combined method (7 kinds of method) was found to be the most discriminative method, followed by (in order) SERE-PCR, REP-PCR, ERIC-PCR, PFGE & OPB-17 (RAPD), P-1254 (RAPD), and BOX-PCR at the $80\%$ clone cut-off value. This finding suggests that the REP-PCR method (which utilizes 4 primer types) may be an alternative tool to PFGE for the genotyping of S. typhimurium isolates, with comparable cost, time, and labor requirement. The establishment of a highly reliable and discriminatory method for epidemiologic analysis is considered necessary in order for researchers to trace the sources of specific pathogens and, consequently, to control and prevent the spread of epidemic S. typhimurium isolates to humans.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2012.06a
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• pp.273-274
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• 2012

Companies are facing challenges to have high competitiveness because of continuous oil price rising and CO2 emissions regulations. Thus, companies are trying hard to construct effective logistics and operation system to achieve high customer service quality and saving cost. Also the ec-friendly idustrial complex is needed. Busan is in process to construct GILC(Global Industry Logistics City) in west Busan province to achieve high competitiveness and support lack of industrial complex. To construct this kind of logistics industrial complex, it needs logistics system through proper policy and freight transportation co-operation. Especially, efficient management through logistics hierarchy construction in industrial complex is very important for low cost and eco-friendly point of view. Therefore, this paper aims to analyze logistics system and suggest operation model to present logistics complex construction base data.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.06a
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• pp.221-222
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• 2014

Companies are facing challenges to have high competitiveness because of continuous oil price rising and CO2 emissions regulations. Thus, companies are trying hard to construct effective logistics and operation system to achieve high customer service quality and saving cost. Also the ec-friendly idustrial complex is needed. Busan is in process to construct GILC(Global Industry Logistics City) in west Busan province to achieve high competitiveness and support lack of industrial complex. To construct this kind of logistics industrial complex, it needs logistics system through proper policy and freight transportation co-operation. Especially, efficient management through logistics hierarchy construction in industrial complex is very important for low cost and eco-friendly point of view. Therefore, this paper aims to analyze logistics system and suggest operation model to present logistics complex construction base data.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.2
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• pp.334-344
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• 2009

The EPCglobal Network is a system which provides the information of items to suppliers, consumers, and customers by granting identification numbers on the goods based on the technologies of EPC(Electronic Product Code) and RFID(Radio Frequency Identification), and connecting the space to store those information by network. The EPCglobal architecture framework is also a service consolidating supply and process chain by using EPC for the common goal of business corporations. In the national level, it is necessary to define standard vocabularies for each location and logistics business process for RFID environment using the pre-constructed logistics information infrastructure. Therefore, we define the standard vocabularies and partially user vocabularies for international logistics on EPCglobal network architecture. Finally, we believe that our vocabularies contribute to describing the events fur the EPCglobal network architecture and actually providing usable platform via the XML implementation.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.6 no.4
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• pp.95-110
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• 2011

Deepening of the global competitive environment due to changes in operating environment and business, domestic and abroad companies in automotive industry are trying to optimize their production and logistics, to enhance their supply chain competitiveness and to promote information strategic planning to maximize their IT-enabled capabilities. In particular, the automotive industry is showing the various efforts to build the demand-based production system for responding to the market changes and preparing the global production and sales system. Despite of their various effors, the IT deployment and utilization levels of parts suppliers in the field of production/logistics companies are shown as higher in comparison with other area supplies, but the levels are shown as a very low condition compared to the parts suppliers of other manufacturing sectors. In this sense, the subject of this study, 'A' company (SMEs), has the challenges for improving the IS utilization and the accuracy of the information system according to the inefficiency of manual and field validation. Consequently, to improve and enhance the utilization of information systems, A company is carrying out the project to improve the ERP and POP operations by in-house approach, but they have shown the limitation in introducing and applying the latest technology.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1044-1053
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• 2012

The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est5S) was 1,026 bp in length, encoding a protein of 366 amino acid residues with a calculated molecular mass of 40,168 Da. The molecular mass of the enzyme was estimated to be 40,000 Da. The Est5S protein contains the Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic serine hydrolases. However, the Asp or Glu necessary for the catalytic triad [Ser-Asp-(Glu)-His] was not present, indicating Est5S represents a novel member of the GHSQG family of esterolytic enzymes. BlastP in the NCBI database analysis of Est5S revealed homology to hypothetical proteins and it had no homology to previous known lipases and esterases. Est5S was optimally active at pH 7.0 and $40^{\circ}C$. Among the p-nitrophenyl acylesters tested, high enzymatic activities were observed on the short-chain p-nitrophenyl acylesters, such as p-nitrophenyl acetate, etc. The conserved serine residue ($Ser_{190}$) was shown to be important for Est5S activity. The primers that amplified the est5S gene did not show any relative band with 49 species of culturable rumen bacteria. This implies that a new group esterase gene, est5S, may have come from a noncultured cow rumen bacterium.

• The Journal of the Korea Contents Association
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• v.13 no.5
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• pp.183-190
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• 2013

In this study, we propose a new CT classification system which is essential factor for strengthening competitiveness of culture contents and technical assistance of convergence contents. For this, we drew particularity of culture contents by reviewing concept and current state of CT. And also we drew problems of existing CT classification systems and implications for applying to future CT classification system by systematic analysis of existing CT classification systems. Through this series of process we developed a new CT classification system applied particularity of culture contents. The suggested CT classification system can embrace all kind of technologies related to all steps of value chain-planning, manufacturing/production, distribution, service- of culture contents. And it has significance having flexibility for government investigation and applying the law and more realistic for industrial site by restructuring of middle classification level.

• Journal of KIISE:Computing Practices and Letters
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• v.12 no.6
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• pp.339-348
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• 2006

Recently, many researches on detecting and responding worms due to the fatal infrastructural damages explosively damaged by automated attack tools, particularly worms. Network service vulnerability exploiting worms have high propagation velocity, exhaust network bandwidth and even disrupt the Internet. Previous worm researches focused on signature-based approaches however these days, approaches based on behavioral features of worms are more highlighted because of their low false positive rate and the attainability of early detection. In this paper, we propose a Distributed Worm Detection Model based on packet marking. The proposed model detects Worm Cycle and Infection Chain among which the behavior features of worms. Moreover, it supports high scalability and feasibility because of its distributed reacting mechanism and low processing overhead. We virtually implement worm propagation environment and evaluate the effectiveness of detecting and responding worm propagation.