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Seroprevalence of Coxiella burnetii in Korean native goats in Gyeongnam province (경남지역 사육 염소 큐열 항체 양성률 조사)

  • Seong, Min-Ho;Bak, Jong-Sik;Youn, Do-Kyung;Kim, Hyeong-Su;Ko, Byeong-Hyo;Ham, Jeong-Min;Jeong, Myeong-Ho
    • Korean Journal of Veterinary Service
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    • v.43 no.4
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    • pp.211-216
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    • 2020
  • Coxiella burnetii is the causative agent of Q fever which is a zoonosis occuring in both humans and animals worldwide. The purpose of this study was to investigate the seroprevalence of C. burnetii in Korean native goat in Gyeongnam province, Korea. A total of 1,365 goat blood samples from 273 farms in Gyeongnam province were collected between 2018 and 2019. Among them, 177 (13.0%) samples out of 71 (26.0%) farms were seropositive for C. burnetii by ELISA. Seroprevalence were 15.4% and 10.9% in 2018 and 2019, respectively. According to the region, seroprevalence in western, central, eastern, northern and southern areas of Gyeongnam province were 16.6%, 17.8%, 8.0%, 11.6% and 10.8%, respectively. Seroprevalence was increased with breeding scale (Head<10:7.0%, 10≤Head<50:8.7%, 50≤Head<100:13.6%, 100≤Head:28.8%). Seroprevalence according to the season showed highest in summer (18.9%) and lowest in winter (9.4%). These results indicated that C. burnetii infection is widespread among Korean native goats of Gyeongnam province in Korea and further study needs to prevent the circulation of other livestock with Korean native goat.

Lowering Error Floor of LDPC Codes Using an Improved Parallel WBF Algorithm

  • Ma, Kexiang;Li, Yongzhao;Zhu, Caizhi;Zhang, Hailin;Zhang, Yuming
    • ETRI Journal
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    • v.36 no.1
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    • pp.171-174
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    • 2014
  • In weighted bit-flipping-based algorithms for low-density parity-check (LDPC) codes, due to the existence of overconfident incorrectly received bits, the metric values of the corresponding bits will always be wrong in the decoding process. Since these bits cannot be flipped, decoding failure results. To solve this problem, an improved parallel weighted bit flipping algorithm is proposed. Specifically, a reliability-saturation strategy is adopted to increase the flipping probability of the overconfident incorrectly received bits. Simulation results show that the error floor of LDPC codes is greatly lowered.

Design and Implementation of a Component Repository System based on a CBD Methodology (CBD 기반 컴포넌트 리파지토리 시스템 설계 및 구현)

  • Park, Chang-Sup;Yeon, Seung-Ho;Rhee, Hae-Kyu;Park, Hyun-Gyu;Lee, Sang-Soo
    • Proceedings of the Korea Information Processing Society Conference
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    • 2003.05b
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    • pp.1003-1006
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    • 2003
  • 본 논문에서는 KT 서비스개발연구소에서 개발된 컴포넌트 리파지토리 시스템의 설계 및 구현방법을 소개한다. 본 시스템은 CBD 방법론 및 EJB 컴포넌트 모델을 적용하여 유연하고 확장성 높은 컴포넌트 기반 시스템으로 개발되었다. 본 시스템은 컴포넌트를 기술하고 검색하기 위한 컴포넌트 명세 방법과 컴포넌트들을 체계적으로 분류, 관리할 수 있는 계층적 분류 체계를 정의한다 또 컴포넌트의 재활용을 위해 효과적인 검색 및 탐색 방법을 제공하며, 사용자 관리 및 통계 기능 둥을 포함한다. 본 논문에서는 요구사항 분석, 설계, 구현 단계에서 CBD 방법론의 적용 방안을 기술하고, 특히 컴포넌트 식별 및 컴포넌트 구조 설계 방법에 대해 상세히 기술한다.

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Fludarabine and Rituximab in Relapsed or Refractory Hairy Cell Leukmia Variant: A Case Report and Review of Literature

  • Lee, Ji Won;Choi, Youn Mi;Yang, Jae Yun;Han, Seung Moon;Kim, Bong Seog;Nam, Seung-Hyun
    • Kosin Medical Journal
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    • v.33 no.3
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    • pp.438-445
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    • 2018
  • Hairy cell leukemia (HCL) is a rare chronic B cell leukemia morphologically characterized by cells with an abundant cytoplasm and hair-like projections that can be found in the peripheral blood and bone marrow. The treatment for HCL is splenectomy or chemotherapy with the purine analogs pentostatin and cladribine. However, patients continue to relapse. Retreatment with the same or alternate purine analogs produces lower response rates and a shorter duration of response. Fludarabine is another purine analog widely used in treating indolent lymphoid cancers, often in combination with rituximab. Here, we report a case of HCL variant in a 60-year-old man who experienced multiple relapses after splenectomy and retreatment with cladribine. The patient was then treated with fludarabine and rituximab combination chemotherapy. After the treatment, he achieved complete remission that continued for 35 months.

MLVA typing of Brucella canis isolated from dogs in Gyeongbuk province, Korea (경북지역 개에서 분리된 Brucella canis의 MLVA-16 typing)

  • Kim, Seong-Guk;Sohn, Joon-Hyung;Jang, Young-Sul;Kim, Sang-Yoon
    • Korean Journal of Veterinary Service
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    • v.35 no.4
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    • pp.275-281
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    • 2012
  • Canine brucellosis is the zoonosis in worldwide and Brucella (B.) canis is a facultative intracellular pathogen that has a very limited host. MLVA-16 (Multilocus VNTR analysis) is a efficient method for genotyping of Brucella species. Various methods have been established for genotyping of Brucella species, but most of analytical method is lack reproducibility and limited capability to differentiate them. B. canis isolates (n=73) from 7 farms in Gyeongbuk province in 2003~2010 were analyzed using 16 VNTR loci. Automatic electrophoresis system was utilized for more high throughput and rapid simple discrimination. Thirty two genotypes were identified from 73 B. canis isolates. MLVA could contribute to molecular typing for epidemiological evaluation of canine brucellosis.

PFGE patterns of Streptococcus suis isolates from diseased pigs in Gyeongbuk province, Korea (경북지역 환돈 유래 Streptococcus suis의 PFGE 패턴 실태 조사)

  • Kim, Seong-Guk;Kim, Young-Hoan;Lee, Hong-Young;Yun, Mun-Jo
    • Korean Journal of Veterinary Service
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    • v.35 no.4
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    • pp.283-288
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    • 2012
  • Streptococcus(S.) suis is a pathogen, causing meningitis, septicemia and sudden death in weaning piglets as well as fattening pigs. Using multiplex PCR method based S. suis capsular genes, 61 S. suis isolates was classified as serotypes 2, 7, 9 and untypable. Genotyping of S. suis isolates was analysed by PFGE pattern with treated Sma I restricted enzyme. Of the 61 S. suis, 25 (40.9%) were serotype 2, 6 (9.8%) were serotype 7, 5 (8.2%) were serotype 9, and 25 (40.9%) were untypable, respectively. Twenty four PFGE patterns were detected in this study and also PFGE patterns were classified according to serotype; serotype 2 was classified as 6 genotypes, serotype 7 was 5 genotypes, serotype 9 was 3 genotypes, and untypable was 11 genotypes, respectively.

Comparison of fatty acid composition of Staphylococcus sp isolated from bovine mastitis milk (유방염 감염 우유에서 분리된 Staphylococcus sp의 지방산 조성 비교)

  • Kim, Soon-Tae;Kim, Sin;Kim, Sang-Young;Son, Jae-Kweon
    • Korean Journal of Veterinary Service
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    • v.20 no.1
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    • pp.37-45
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    • 1997
  • The result of API staph-ident system was compared with cellular fatty acid composition for the identification of Staphylococcus species isolated from cattle. Isolated strains from cattle were correctly identified to S aureus, S intermedius, S hyicus, S simulans, S saprophyticus, S epidemis, S sciuri and S xylosus by API staph-ident system. The correlation between bacterial cellular fatty acid profile and Staphylococcus species isolated to API STAPH-IDENT system were. In conclusion, the result presented indicate that Staphylococci can be indentified to the species level by the cellular fatty acid profiles. Moreover, computerized fatty acid profile correlative anaylsis can be applied for determining identify of Staphylococcus species.

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A comparative study on the diagnosis of ELISA test and PPD test of the bovine tuberculosis (ELISA를 이용한 우결핵검사 결과에 대한 PPD 접종법 결과 분석 비교)

  • Lee, Jong-Jin;Kim, Duk-Soon;Lee, Jong-Hwa;Lee, Cheong-San
    • Korean Journal of Veterinary Service
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    • v.33 no.4
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    • pp.335-340
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    • 2010
  • On the basis of the 2009 business plan, 20,394 Korean native cattle and beef cattle were carried examination of bovine tuberculosis by using ELISA technique from March to December. As a result, 66 cattle tested positive for tuberculosis and showed 0.32% positive ratio. Intradermal tuberculin test about 66 cases of ELISA positive cattle was carried out, and all of 66 cattle were confirmed as negative. However, when 7 PPD-positive cattle derived from slaughterhouse were tested by 20k ELISA kit and MS ELISA kit, 3 (2 suspect) cattle and 5 cattle showed positive results, respectively. As compared to the results of PPD test, the concordance rates were 43% (71% included suspect) with 20k ELISA kit and 71% with MS ELISA kit.

Characteristics of antimicrobial resistant Salmonella Typhimurium isolates from pigs (돼지에서 분리한 Salmonella Typhimurium의 항생제 내성 양상)

  • Sohn, Jun Hyung;Kim, Mee Suk;Lee, Young Mi;Do, Jae Cheul
    • Korean Journal of Veterinary Service
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    • v.40 no.2
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    • pp.83-87
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    • 2017
  • 59 strains of Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) were isolated from pigs in Gyeongbuk province, collected from 2011 to 2016. The isolates were investigated for the presence of antimicrobial resistance and multi drug resistance patterns. All 59 S. Typhimurium isolates were resistant to at least one of 10 antibiotics used in this study, 100% of S. Typhimurium isolates from pigs were resistant to two or more antimicrobials. As many as 5 isolates of isolates from pigs were resistant to 8 of 10 antimicrobials tested in this study. The ACSTNaGmKNaCf, ACSTGmAuKT/S, ACSTGmKCfT/S resistance phenotype was observed in 3.4%, 3.4%, 1.7% of the 59 isolates, respectively.

MALDI TOF MS for the identification of Salmonella spp. from swine (돼지유래 Salmonella속 균의 동정을 위한 MALDI TOF MS 활용)

  • Sohn, Jun Hyung;Jeon, Woo Jin;Lee, Young Mi;Kim, Seon Soo
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.247-251
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    • 2016
  • Salmonella is one of the most common bacteria that causes heavy losses in swine industry and major causative pathogen of food poisoning in public health. Various methods for the identification of Salmonella such as Gram staining, agglutination test, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) have been used. Several studies have demonstrated that Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Mass Spectrometry (MS) identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. In this study, MALDI TOF MS could provide rapid, accurate identification of Salmonella spp. from swine compared with end point PCR and real time PCR.