• 한국응용과학기술학회지
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• 제40권3호
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• pp.385-391
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• 2023

본 연구는 혈중 비타민 E 농도와 대사증후군의 관련성을 살펴보고 비타민 E의 신체에 미치는 영향을 살펴보고자 한다. 분석 자료는 질병관리본부에서 시행한 국민건강영양조사 제7기 3차 (2018년도) 자료를 사용하였으며 본 연구에서는 만 19세 이상 성인은 대상으로 혈중 비타민 E의 농도를 검사한 대상자 중 남자 944명, 여자 1,080명을 최종 분석하였다. 자료 분석은 일반적 특성에 따른 혈중 비타민 E 농도 차이는 Chi-square와 t- test로, 혈청 비타민 E 농도와 대사증후군 하부요인들 간의 관계는 Pearson 상관관계로, 혈중 비타민 E 농도와 관련된 대사증후군 지표는 로지스틱 회귀분석을 하였다. 대사증후군의 하부요인과 혈중 비타민 E의 회귀분석 결과 HDL-cholesterol이 정상군이 과잉군에 비해 높았으며(OR:1.042, CI:1.028-1.056), 중성지방도 정상군에 비해 유의미하게 높았다(OR:1.010, CI:1.009-1.012)(p<0.01). 따라서 본 연구를 통하여 환자의 연령에 맞는 영양 및 건강프로그램을 개발할 것을 제안한다.

• Clinical and Experimental Vaccine Research
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• 제12권2호
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• pp.97-106
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• 2023

Purpose: Rabies is a fatal but preventable disease with proper pre-exposure anti-rabies vaccination (ARV). Dogs, as household pets and strays, are the reservoir and vector of the disease, and dog bites have been associated with human rabies cases in Sri Lanka over the past few years. However, other susceptible species having frequent contact with humans may be a source of infection. One such species is sheep and immunity following ARV has never been tested in sheep reared in Sri Lanka. Materials and Methods: We have tested serum samples from sheep reared in the Animal Centre, Medical Research Institute of Sri Lanka for the presence of anti-rabies antibodies following ARV. Sheep serum samples were tested with Bio-Pro Rabies enzyme-linked immunosorbent assay (ELISA) antibody kits used for the first time in Sri Lanka and our results were verified by a seroneutralization method on cells (fluorescent antibody virus neutralization, FAVN test) currently recommended by World Organization for Animal Health and World Health Organization. Results: Sheep received annual ARV and maintained high neutralizing antibody titers in their serum. No maternal antibodies were detected in lamb around 6 months of age. Agreement between the ELISA and FAVN test, i.e., coefficient concordance was 83.87%. Conclusion: Annual vaccination in sheep has an effect on maintaining adequate protection against rabies by measurements of anti-rabies antibody response. Lambs need to be vaccinated earlier than 6 months of age to achieve protective levels of neutralizing antibodies in their serum. Introducing this ELISA in Sri Lanka will be a good opportunity to determine the level of anti-rabies antibodies in animal serum samples.

• Journal of Nutrition and Health
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• 제35권1호
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• pp.45-52
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• 2002

This study was to investigate 1) the correlation of serum Ca, with depression and anxiety ; and 2) the effect of Ca intake on those symptoms. The subjects were three females and two males who were 53-66 years old with the severe subjective symptoms of depression and anxiety. They have taken more than twice Ca of RDA(recommended dietary allowances for Koreans) daily for 6 months(dietary treatment). The prestudy Ca intake of the subjects was low: 60% of RDA for Koreans. Their serum Ca concentration was also low: 6.67 $\pm$ 0.15mg/d1 before the dietary treatment. They expressed severs depression and anxiety, with high self scores in a psychological test. Their serum Ca concentration increased to 8.32 $\pm$ 0.17mg/dl after six months of dietary treatment, while the symptoms of depression and anxiety decreased significantly after two months and nearly disappeared after six months. This result seemed to be an effect of the dietary high Ca intake. Serum Ca and the psychological states of depression and anxiety correlated negatively, and the coefficients of determination were high in the results of linear regression analysis of depression and anxiety by serum Ca. Therefore the serum Ca concentration could be a good marker to predict depression or anxiety relatively well. The continuous high Ca intake could decrease the probability of developing depression or anxiety and mitigate their symptoms because serum Ca concentration increased, while the state of depression and anxiety decreased with the increased Ca intake.

• 한국간호교육학회지
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• 제10권2호
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• pp.289-296
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• 2004

The purpose of this study was examine the relationship between smoking, general characteristics, and serum lipids in the normal adult. The subjects consist of 6,800 persons (male ; 4,309(63.4%), female ; 2,491(36.6%)) in A hospital in Kyungki-Do. The collected data were analyzed using T-test, and Multiple Regression by SPSS/WIN program. The result were following ; 1. Cigarette smoking was associated with decreased serum HDL cholesterol level (p<.001). 2. Serum LDL cholesterol level was not statistically correlated with smoking tend to level by 5.77mg/dl respectively. 3. Cigarette smoking was associated with elevated serum Triglyceride level (p<.001). 4. By using regression, HDL cholesterol level was negatively correlated with obesity and alcohol.. HDL cholesterol level was positively correlated with exercise. LDL cholesterol level was positively correlated with diet and obesity.　Triglyceride level was positively correlated with obesity and　alcohol. In conclusion, Smoking was the most correlated with Triglyceride among the serum lipid and Serum lipid was the most correlated with obesity. Thus, it is necessary to quit smoke and weight control for prevention from disease such as coronary heart disease and lung cancer.

• Korean Journal of Acupuncture
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• 제25권3호
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• pp.65-80
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• 2008

Objective : In recent years, many investigators have questioned whether the analgesic effect of acupuncture is simply related to the stress-induced analgesia (SIA). However, there has been lack of studies on this issue. In this study, the stress levels induced by manual acupunctures are compared with the stress in animal experiment models. The experiments have been carried out with Sprague Dawley (SD) rats. Method : For stress level evaluation, Hot plate test has been used. Maximum Possible Effect (MPE) has been measured by checking the pre-test time and post-test time. Cortisol and corticosterone concentrations in serum were measured by enzyme-linked immunosorbent assay (ELISA). Results : In the hot plate test, MPE values of post-test time were significantly decreased after 10 minutes than after 5 minutes. Therefore, optimal time interval was chosen as 10 minutes. There was significant difference of MPE values between Suspension group and all other treatment groups. However, there were no significant differences of MPE values between Sham group and all other treatment groups. However, MPE values showed tendency to decrease when acupuncture needle diameter increased. MPE values of ST040, ST040(lido), NAP040(lido) groups were markedly decreased than that of Suspension group, while that of NAP040 group was substantially increased than that of Sham group increased in acupoint and nonacupoint models. Serum cortisol concentrations of treatment groups were not significantly different from that of Suspension and Sham groups. Serum corticosterone concentration of 0.25 mm group was substantially increased than that of compared with Sham group. Serum cortisol and corticosterone concentrations of treatment groups were not significantly different from those of Suspension and Sham groups in acupoint and nonacupoint models. Conclusion : From hot plate test and serum stress hormones concentrations, it is found that manual acupuncture treatment induces negligible stress or SIA on ST36. And the stress induced by manual acupuncture is more closely related to acupuncture point needlings than diameters of acupuncture needles.

• 한국임상수의학회지
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• 제17권1호
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• pp.212-218
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• 2000

Osteoporosis is a bone disease associated with reduced bone mineral density resulting in debilitating bone fractures. The present study was carried out to determine the influence of ovariectomy(OVX) on serum level of sex steroid and bone metabolism, as well as bone mechanical property, in OVX subjects in comparison with controls. Body weight and food intake was significantly increased in OVX subjects than in controls. The serum level of estradiol(E2) was significantly lower in OVX subjects than in controls. The serum level of calcium and alkalin phosphatae were significantly increased in OVX subjects than in controls. The bone strength, based on the three point bending test, was not significantly different. Seven weeks after ovariectomy, the cavities in the bone reached 223% of the normal. In conclusion, seven weeks after ovariectomy osteoporo sis was evidently appeared.

• 대한수의학회지
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• 제16권1호
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• pp.7-10
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• 1976

An agglutinability between naturally infected positive chicken serum of pullorum disease and hyperimmumized rabbit antiserum was compared. And the following results were obtained and summarized. 1. On the agglutinability, Salmonella pullorum antigen which irradiated gamma-ray was more excellent than another both formalized and heated antigen. 2. Time of judgemented as positive titer in the tube agglutination test to the naturally infected positive chicken serum was it most suitable for 12 hours at $37^{\circ}C$. 3. Agglutination titer of positive immune chicken serum against gamma-ray irradiate Salmonella pullorum were as 320~640x.

• 한국동물위생학회지
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• 제42권4호
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• pp.305-311
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• 2019

The objective of this study was to determine the safety test for propolis in Beagle based on blood count, serum biochemistry, and electrolyte. Total six beagle dogs were assigned to this experiment. To investigate the safety of propolis in beagle dogs, we performed oral administration of propolis (5%) for 8 weeks. Among six beagles, three beagle dogs were randomly allocated to the control group which were fed only regular fodder, and the other three dogs were assigned as the treatment group which were fed regular fodder and propolis (5%). No clinical signs were observed in neither group throughout the experimental period. During the experimental period, there were no significant change in feed intake, water consumption, and body condition. Also, there were no statistically significant differences in hematological and biochemical analyses between the control group and the treated group. Our safety study showed that oral consumption of propolis did not cause any toxicological effects in beagle dogs.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권5호
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• pp.362-365
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• 2000

A rapid immunochromatographic assay kit using whole blood to screen hepatitis B surface antigen was developed and evaluated by using sera from 240 patients. The reference diagnosis was based on the results obtained with GENEDIA Anti-HBs Rapid kit which is very similar to the above kit except for the use of serum. The test demonstrated a good correlation with the reference immunochromatographic assay kit, that is, the sensitivity and the specificity of the kit was 100%, respectively. The rapid test kit using whole blood should be more convenient and useful for the diagnosis of hepatitis B virus because the kit does not need machines and time to prepare serum. In addition, this kit is safe from inadvertent infection during sample treatment because the blood is sterilized with hydrogen peroxide, eliminates the procedure required to prepare serum and reduces the possibility of exposure to infectious agents.

• 한국임상수의학회지
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• 제15권1호
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• pp.46-55
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• 1998

This study was performed to verify the effect of modified live virus vaccine against canine parvovirus (CPV) infection. Serum hemagglutination inhibition (Hl) test, histopathological and immunohistochemical techniques and polymerase chain reaction were used. The results were as follows: 1. During the experimental terms after vaccination, serum Hl titer was stable. Geometric mean titer (GMT) after the 1st vaccination was 280. After virulent CPV was challenged, GMT was 1,306. 2. After challenge by virulent CPV, the vaccinated group was not shown clinical signs and gross and histopathological findings. 3. After challenge by virulent CPV, the vaccinated group was not detected viral antigens in the small intestine immunohistochemically. 4. After challenge by violent CPV, the vaccinated group was not shown virus shedding in feces. In conclusions the overall results confirmed that modified live virus vaccine was effective on prevention of canine parvovirus infection.