• 한국콘텐츠학회논문지
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• 제9권4호
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• pp.236-246
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• 2009

알레르기 환자의 증가로 인하여 알러겐을 찾는 것은 중요하다. MAST는 알러겐 특이 IgE항체를 측정하는 간단한 방법이다. 본 연구에서는 알레르기 환자에서 알러겐 특이 IgE 항체, 총 IgE, 호산구수와의 관련성을 평가하고자 하였다. 연구 결과 총 IgE 양성률은 흡입형 패널에서 96.97%, 식품형 패널에서 98.06%였다. 알러겐 특이 IgE 항체의 양성률은 흡입형 패널에서 집먼지 51.52%, 북아메리카 집먼지 진드기 45.46%, 고양이 31.99% 였으며, 식품형 패널에서 각각 55.34%, 42.72%, 34.96%였다. 혈청 총 IgE는 알레르기와 관련이 있었으나, 알레르기가 항상 호산구증가와 관련이 있는 것은 아니었다.

• 대한한방내과학회지
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• 제32권1호
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• pp.10-25
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• 2011

Objectives : Asthma is considered an inflammation disease characterized by airway hyperresponsiveness and pulmonary eosinophilia. Chungsang boha-tang is a herbal medication which has been frequently used in traditional therapeutic agents for asthma. We aimed to observe the clinical effects of Chungsangboha-tang on blood eosinophil, serum IgE, AST, ALT and pulmonary function in asthmatic outpatients. Methods : The subjects consisted of 66 asthmatic patients who had been treated with Chungsangboha-tang among all the asthmatic patients who visited the 5th Internal Medicine of the Oriental Medicine Hospital of Kyung Hee University from November,2004 to November,2009. We observed blood eosinophil, serum IgE, AST, ALT levels and pulmonary function test results as well as medical records of patients to evaluate the effects. Results : Pulmonary function significantly improved after 8 weeks of treatment, and blood eosinophil and serum IgE level significantly decreased after 8 weeks of treatment. AST, ALT levels changed in normal ranges. Chungsangboha-tang was significantly effective on the male patients who had a smoking history and worse initial lab findings. We also observed a significant improvement of pulmonary function and decrease of eosinophil and serum IgE levels in the patient group who had a single treatment of Chungsangboha-tang without steroid therapy. Conclusions : This study shows that Chungsangboha-tang has effects on the improvement of pulmonary function and decrease of eosinophil and serum IgE levels as well as improvement of symptoms. And, 8 weeks of long-term treatment showed no negative effects such as significant increase of AST, ALT levels.

• Parasites, Hosts and Diseases
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• 제35권2호
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• pp.87-94
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• 1997

우리 나라에서 가장 감염자가 많은 기생충인 간흡충의 현 감염에 특이한 항원 분획을 찾고자 면역이적법을 이용하여 감염자의 혈청을 검색하였다. 실험적으로 토끼에서 얻은 간흡충 성충의 조항원에 시스테인계 단백질분해효소 억제제인 I-64를 첨가하였을 때 큰 분자량의 분획을 가장 잘 보존하여 200-14 kDa의 범위에 20개 이상의 분획을 관찰하였다. 이 조항원을 이용하여 경북 상주군 주민의 혈청을 면역이적법으로 검사하였다 대변검사, 피내반응검사, 효소면역법검사(ELISA)를 이 용하여 검사한 73명 중 충란양성자 49명의 혈청 내에 특이 IgG와 IgE 항체가 생성되었고, IgM과 IgA 항체는 특이하게 반응하지 않았다. 여러 항원 분획 중에서 43, 34, 28-25 kDa 항원이 현 감 염에 특이한 분획이고. 항체와 반응한 94, 80, 72, 68, 64, 62, 52, 47, and 40 kDa 분획은 특이하지 않은 분획임을 확인하였다. 추후에 각 항원과 이에 대한 혈청반응에 대하여 특성을 더 연구 할 필요가 있으며, 특히 치료후 추적검사를 통하여 혈청내 항체의 소실을 구명하여야 할 것이다.

• Journal of Acupuncture Research
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• 제18권1호
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• pp.29-39
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• 2001

Purpose : It has been reported that Oyo-Tang has an effect on allergic asthma clinically. In order to investigate the herbal acupuncture solution of the Oyo-Tang on allergic asthma, the Oyo-Tang herbal-acupuncture(OTHA) was applied on BL13 and ST36 of allergic asthma model of the rats. Methods : Then the concentration of serum IgE and the ratio of CD4+ and CD8+ T-cells in peripheral blood were measured and compared with control group. Results : The results obtained as follows; 1. The concentration of serum IgE was decreased significantly in both of the OTHA on BL13 group and on ST36 group as compared to control group. 2. The ratio of CD4+ and CD8+ T-cells in peripheral blood was not different statistically between the normal, the control, the OTHA on BL13 and the OTHA on ST36 group.

• Allergy, Asthma & Respiratory Disease
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• 제6권6호
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• pp.310-314
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• 2018

Purpose: Conventional serum IgE assay was costly, required the skills of expert, and relied heavily on expensive equipment. Quantitative measurement of total IgE using Point of Care Test (POCT) device can be the solution for these limitations. This study evaluated and validated the reproducibility of ImmuneCheck IgE. Methods: This study included 120 patients of allergic diseases such as allergic rhinitis, asthma, drug allergy, food allergy, atopic dermatitis, or anaphylaxis. The reliability of POCT ImmuneCheck IgE was evaluated by comparing results from the naked eye and from the Q-Reader. Intratest reproducibility and intertest correlation were analyzed using intraclass correlation coefficient (ICC). Results: Of the 120 enrolled patients, 51 were males and 69 were females. The ages ranged from 19 to 84 years, with an average age of 51.5 years. The concentration of serum total IgE measured by Phadia ImmunoCAP IgE ranged from 5.95 to 5,000 IU/mL. ICC for Intratest reproducibility of ImmuneCheck IgE by naked eye and by Q-Reader were 0.991 (P< 0.001) and 0.989 (P< 0.001), respectively. In addition, intertest correlation between ImmuneCheck IgE and Phadia ImmunoCAP IgE results of naked eye and Q-Reader were 0.968 (P< 0.001) and 0.948 (P< 0.001), respectively. Conclusion: The ImmuneCheck IgE was reproducible and highly correlated with conventional Phadia ImmunoCAP IgE assay. This result suggests that ImmuneCheck IgE can be a useful tool for rapid and precise detection of total IgE.

• Pediatric Infection and Vaccine
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• 제10권1호
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• pp.127-131
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• 2003

Hyperimmunoglobulin E syndrome(HIES) is a primary immunodeficiency characterized by severe recurrent soft-tissue infections, pneumonias, pruritic dermatitis and markedly elevated serum IgE levels. Pneumatocele and empyema develop as result of recurrent pneumonias. It is inherited in an autosomal dominant fashion, with variable expressivity. We experienced the case of a 10 month old girl with hyper IgE syndrome. Patient was admitted for local ulceration and muscle soreness at the site of BCG inoculation. The diagnosis of hyper IgE syndrome was made because she had a coarse facial appearance, pruritic dermatitis, pneumatocele and markedly elevated serum IgE level with a past history of frequent skin abscess and pneumonia.

• 대한본초학회지
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• 제20권2호
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• pp.35-42
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• 2005

Objective : This study was performed to investigate the effect of oral administration of Oldenlandiae Diffusae Herba (ODH) on eosinophil, immunoglobulin-E and interleukin-4 in the experimental asthma induced by ovalbumin. Methods : Asthma was induced to Balb/c mouse by i.p. injection and aerosol immunization with ovalbumin. It was observed the change of the eosinophil number in the BALF. And, it was observed the change of the concentrations of IL-4 in BALF and splenocyte, IgE in serum by ELISA. Results : 1. The number of eosinophil in BALF was significantly decreased in ODH group copared with control group. 2. Concentration of IL-4 in serum, BALF and splenocyte was significantly decreased in ODH group compared with control group, respectively. 3. Level of IgE in serum was significantly decreased in ODH group compared with control group. Conclusion : We found that the effect of ODH extract in asthma was implicated in reductions of IL-4 released from Th2 cell, and decreases of IgE from plasma cell. These findings suggest that ODH extract can produce anti-asthmatic effect, which may playa role in allergen-induced asthma therapy.

• 한국식품과학회지
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• 제40권1호
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• pp.82-87
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• 2008

아토피 피부염에서 유산균의 유익성에 대해서는 이미 많이 알려져 있고 우리나라 전통식품인 김치유산균의 유익성에 대한 연구도 많이 진행되었으나 아토피 피부염에서 김치 유산균의 효과에 관한 연구는 아직 미비한 실정이다. 본 연구에서는 아토피 동물 모델인 NC/Nga mice에 김치유산균에서 추출한 유산균(L.plantarum K8)의 파쇄물과 유산균 및 ${\gamma}$-리놀렌산 복합체(L.rhamnosus GG 생균, B. lactis Bb-12Lb 생균, L. plantarum K8생균, L. plantarum K8 파쇄물, ${\gamma}$-리놀렌산)를 경구 투여한 후 혈청 면역지표(IgE, IL-4, IL-5, IFN-${\gamma}$)와 비장 배양액에서 IgE 농도를 측정하였다. 실험동물의 체중 및 사료섭취량은 사육기간이 지남에 따라 증가하는 경향을 보였으며 각 군 간에 유의적인 차이는 관찰되지 않았다. 실험 시작 시 혈청 IgE 농도는 각 군 간에 차이가 나타나지 않았으나 실험 전 기간 4주 동안 유산균 파쇄물(DPF), 유산균 및 ${\gamma}$-리놀렌산 복합체(DPOC), 항히스타민(DAH)을 섭취한 군에서 증류수(DDW)만을 섭취군에 비하여 유의적으로 낮게 나타났다. 혈청 IFN-${\gamma}$ 농도는 각 군에서 유의적인 차이가 나타나지 않았으나 혈청 IL-4 농도는 유산균 및 ${\gamma}$-리놀렌산 복합체를 섭취한 군(DPOC)에서 대조군에 비하여 유의적으로 낮게 나타났다. 혈청 IL-5 농도는 유산균 파쇄물(DPF), 유산균 및 ${\gamma}$-리놀렌산 복합체(DPOC), 항히스타민(DAH)을 섭취한 군에서 모두 대조군에 비하여 유의적으로 낮았다. 비장 임파구 배양 상층액의 IgE 농도는 각 군에서 군 간 유의적인 차이가 나타나지 않았다. 결론적으로, 아토피 동물모델 NC/Nga mice에 유산균 파쇄물(DPF)과 유산균 및 감마리놀렌산 복합체(DPOC)의 경구투여는 혈청 IgE, IL-4, IL-5를 감소시키는 것으로 나타났으며, 김치로부터 추출한 프로바이오틱스를 아토피 환자에게 섭취시켰을 때 IgE, IL-4, IL-5 감소와 같은 긍정적인 효과를 볼 수 있을 것으로 기대한다. 여러 종류의 프로바이오틱스는 아토피의 증상완화 또는 면역 체계에 미치는 영향과 정확한 메카니즘을 밝히기 위하여 과학적인 연구가 필요한 것으로 사료된다.

• 대한한의학회지
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• 제23권2호
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• pp.198-210
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• 2002

Background : Asthma is a chronic inflammatory disorder under immunological influence. Shinbi-tang and Gamishinbi-tang are herbal decoctions used for treating asthma in traditional herbal medicine. Objective : To evaluate the effects of Shinbi-tang and Gamishinbi-tang on immune cell & serum OA-specific IgE in broncho-alveolar lavage fluid (BALF) in rat asthma model. Material and Methods : Rats were sensitized with ovalbumin (OA); at day 1 sensitized group and Shinbi-tang and Gamishinbi-tang groups were systemically immunized by subcutaneous injection of 1mg OA and 300mg of Al(OH)$_3$ in a total volume of 2ml. At the same time, 1 ml of 0.9% saline containing 6 x 10$^{9}$ B. pertussis bacilli was injected by i.p. 14 days after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerosol containing 2%(wt/vol) OA. A day after local immunization, HAL fluid was collected from the rats. Rats were orally administered with each of Shinbi-tang and Gamishinbi-tang extract for 14 days from the day after local immunization. Lymphocyte, CD4+ T cell CD8+ T cell counts, CD4+/CD8+ ratio in BALF, change of serum OA-specific IgE level, CD4+ T cell CD8+ T cell percentages in the peripheral blood were measured and evaluated. Results : Shinbi-tang and Gamishinbi-tang showed an alleviating effect on asthmatic responses of rats. Shinbi-tang decreased total cell, lymphocyte, CD4+ T cell in BALF, serum OA-specific IgE level as compared with the control group. Gamishinbi-tang decreased total cell, lymphocyte, CD4+ T cell, and CD4+/CD8+ ratio in HALF as compared with the control group. CD4+/CD8+ ratio in HALF from Shinbi-tang group and serum OA-specific IgE level from Gamishinbi-tang group didn't show any significant variation from control group. CD8+ T cell in HALF, CD3+CD4+ T cell and CD3+CD8+ T cell percentages in peripheral blood showed no significant variation among groups. Conclusion : Shinbi-tang and Gamishinbi-tang alleviated asthmatic hypen-eactivity of the rat immune system through CD4+ T cell and serum IgE. Further the study of immune system modulating mechanism is expected.

• Parasites, Hosts and Diseases
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• 제35권1호
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• pp.47-54
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• 1997

윤충 감염시 증가되는 기생충 특이 IgE 항체는 항기생충 작용을 하는 것으로 알려져 있으나 총 IgE 항체의 90% 이상을 차지하는 비특이성 IgE 항체의 역할에 대해서는 잘 알려져 있지 않다 이 실험에서는 폐흡충 감염 마우스에 anti-lgE 단일크론 항체를 처치한 후 혈청내 IfgE. 비장 B 세포 의 $Fc{\varepsilon}RII/CD23$의 발현 및 충체수를 관찰하여 비특이성 IgE가 숙주의 방어면역에 미치는 역할을 알아보았다. Anti-lgE를 처치한 폐흡충 감염 마우스의 혈청내 총 IgE 항체의 양은 실험 기간 동안 억제되어(($<{\;}1{\;}{\mu\textrm{g}/ml}$) PBS로 처치한 감염군에 비해 유의한 수준(P < 0.05)으로 감소되었다 또 한 Anti-IgE를 처치한 폐흡충 감염 마우스에서의 $Fc{\varepsilon}RII/CD23$ 양성인 비장 B 세포수 및 그 발현도 PBS로 처치한 감염군에 비해 실험 기간 동안 유의한 수준(P < 0.05)으로 감소되었다. 충체 회수율에 있어서도 anti-lgE를 처치한 감염 마우스는 6주에 44%로 PBS로 처치한 감염군(75%)에 비해 유의한 수준(P < 0.05)으로 감소하였다. 이상의 결과를 종합할 때 폐흡충 감염 마우스에서 높게 증가되는 비특이성 IgE는 숙주의 방어면역을 방해하여 오히려 충체를 보호하는 역할을 하는 것으로 생각된다.