• 대한미생물학회지
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• 제17권1호
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• pp.57-66
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• 1982

The correlation between the serotypes and sensitivity distribution of Pseudomonas aeruginosa was studied with stock strains of department, strains isolated from various clinical sources and strains of haspital environment of the past two years. Two hundred and fifty seven strains were typable and 38 strains were untypable out of 295 clinical sorces. Serotype B was most provalent(27.6%) followed by serotype G(24.9%), serotype E(23.3%) and serotype A(7.8%) among 257 typable strains. However, no serotype J,L and M were observed. Serotype B,G,E and A were isolated from pus, sputum, wound, burn site and urine. Serotypes E were most frequently isolated from nasal discharge and serotypes K were isolated from pus. There were no apparent differences in sensitivity distribution of streptomycin, carbenicillin and ampicillin between the clinical and The environmental origin. However, the strains of environmental origin were found to be relatively more susceptable to tetracycline, gentamicin and fradiomycin than the strains of clinical sources in high concentration. The strains of clinical sources of serotype B,G,E and A showed different resistant patterns to all antimicrobial agents except for carbenicillin. Serotype E showed the highest percentage resistance followed by serotype G,B and A.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제3권1호
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• pp.41-46
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• 2000

목 적: 설사 환아에서 rotavirus 장염의 빈도는 매우 높으며 rotavirs에 대한 예방 접종이 실용화되었으나 국내 rotavirus 혈청형 분포에 대한 체계적인 연구 결과는 매우 드물고, 혈청형의 정확한 분포비율도 아직까지는 알려져 있지 않다. Rotavirus 설사 환아의 G 혈청형 분포를 RT-PCR 기법을 이용하여 조사하였다. 대상 및 방법: 1998년 6월부터 1999년 5월까지 설사를 주소로 내원 또는 입원한 소아 202명을 대상으로 하였다. 설사 시작 후 3일 이내의 대변을 채취하여 영하 $20^{\circ}C$의 냉동고에 보존하였다. EIA(TestPack Rotavirus, Abbott Laboratories)법으로 rotavirus 양성인 검체를 선별하였으며, 양성 반응을 보인 검체를 대상으로하여 RT-PCR을 이용하여 rotavirus G serotype을 결정하였다. 결 과: 총 202 검체 중에서 rotavirus 양성은 95례였으며, 이 중 serotype 판정이 가능했던 것은 50례이었다. G serotype 1이 5례(10%), 2 (G2)가 5례(10%), 3 (G3)가 14례(28%), 4 (G4)가 13례(26%), 9(G9)이 10례(20%)였으며, G1과 G9이 함께 있는 것과, G2과 G9이 함께 있는 것, G4와 G9이 함께 있는 경우가 각 1례씩 있었다. G8은 발견되지 않았다. 결 론: 국내에 기존에는 알려지지 않았던 G9이 상당수 있으며, 일반적으로 제일 많다고 알려진 G1보다 G3, G4가 더 많은 비율을 차지하는 등 이번 연구 결과는 기존에 발표되었던 자료와는 많은 상이점을 보인다. 이는 지역적인 요인 및 시기 상의 일시적인 현상이라고 볼 수도 있으나, 보다 정확한 역학적인 자료를 얻기 위해서는 보다 광범위하고 장기적인 조사가 필요할 것으로 판단된다.

• 대한미생물학회지
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• 제14권1호
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• pp.63-69
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• 1979

Antibodies to Propionibacterium acnes(Corynebacterium parvum) serotype I and serotype II in normal human sera were measured using a microtitre bacterial agglutination test. Of 168 sera tested, 53 sera(31.0%) exhibited higher agglutinin titres to serotype I than to serotype II and 34 sera(20.2%) gave higher titers to serotype II than to serotype I. Eighty-one sera(48.3%), however, showed similar antibody titres to both types. Antibodies to serotype I(x) and serotype II(y) showed high correlation(r=0.73, p<0.01) and regression equation was Y=1,078+0.73X. The mean antibody titre($log_2$) of 529 normal sera(male 447 and female 82) to serotype I was $5.49{\pm}1.36$, but there was no significant difference between male($5.45{\pm}1.36$) and female($5.74{\pm}1.36$). Bacterial agglutinin to Propionibacterium acnes in normal sera belonged to a 2-mercaptoethanol resistant IgG class.

• 대한수의학회지
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• 제36권1호
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• pp.181-186
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• 1996

The present study was conducted to investigate the biochemical and serologic characteristic of Actinobacillus pleuropneumoniae isolated from pneumonic lungs of pigs during the period from January 1992 to April 1993. A pleuropneumoniae was isolated from 17(27.0%) of 63 growing pigs with respiratory signs and 21(6.4%) of 330 pneumonic lungs of slaughtered pigs. The seasonal isolation frequency of A pleuropneumoniae was higher in winter and spring than that in summer or fall. The biochemical and cultural properties of A pleluropneumoniae isolated from the pneumonic lungs of pigs were identical to those of the reference strains used. The isolates were highly susceptible to ampicillin, cephalothin, ceftiofur, ciprofloxacin(MIC : ${\leq}0.39{\mu}g/ml$) and moderately susceptible to amikacin, chloramphenicol, erythromycin, kanamycin, methicillin, penicillin-G, streptomycin(MIC : 0.78~25IU or ${\mu}g/ml$), respectively. Sulfadimethoxine, sulfamerazine, tylosine showed no response to the isolates(MIC : ${\geq}100{\mu}g/ml$). Among the 38 isolates, 21(55.3%) and 13(34.2%) were resistant to oxytetracycline aid lincomydn, respectively(MIC : ${\geq}50IU$ or ${\mu}g/ml$). The majority of 38 A pleuropneumoniae isolates were turned out as serotype 2(47.4%) or serotype 5(54.7%) and the remaining 3 isolates were evenly classified to serotype 7, 10 or 12. It was noted A pleuropneumonine serotype 5 isolates were more resistant to oxytetracycline than serotype 2 isolates.

• Journal of Microbiology
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• 제41권1호
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• pp.1-6
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• 2003

The genetic relatedness of multidrug-resistant pneumococcal isolates of serotypes 19F and 23F was investigated. The DNA fragments digested with Sma I were resolved by pulsed-field gel electrophoresis (PFGE). PFGE analysis of 365. pneumoniae isolates showed 13 different patterns. Among 22 isolates of serotype 19F, 9 different PFGE patterns were present and 14 isolates of serotype 23F isolates represented 5 distinct PFGE patterns. Two isolates of serotype 19F and six isolates of serotype 23F shared the same PFGE pattern (Pattern I). Based on the genetic relatedness within the strains (one genetic cluster was defined as having more than 85% homology), we divided the pneumococcal strains into genefic clusters (Ⅰ, II, III, IV, V, and VI). The 22 strains of serotype 19F belonged to five distinct genetic clusters (I, II, III, IV, V and VI) and 14 strains of serotype 23F represented two genetic clusters (I and II ). These results showed that strains of serotype 19F are genetically more diverse than those of serotype 23F, Serotype 19F isolates with PFGE patterns H and I appeared to be less related to those of the remaining PFCE patterns (A to G) (less than 60% genetic relatedness), but those strains were genetically closely related with serotype 23f. These results suggest that the latter isolates originated from horizontal transfer of the capsular type 19F gene locus to 23F pneumococcal genotypes. In conclusion, the multidrug-resistant pneumococcal isolates of serotype 19f and 23F isolated in Korea are the result of the spread of a limited number of resistant clones.

• 대한수의학회지
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• 제31권3호
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• pp.279-284
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• 1991

To investigate the epidemiological aspects of listeriosis, serotypes of L monocytogenes and antimicrobial susceptibilities of Listeria spp isolated from chicken carcases and chicken slaughter house environmental specimens were determined. Of 28 L monocytogenes strains, 12 strains(42.9%) were serotype 4, and the remaining 16 strains were untypable. Peak distributions of minimum inhibitory concentration$({\mu}g/ml)$ of the isolates were $0.78{\mu}g/ml$ for ampicillin, $0.39{\mu}g/ml$ for erythromycin and penicillin G, $1.56{\mu}g/ml$ for tetracycline and $6.25{\mu}g/ml$ or $12.5{\mu}g/ml$ for chloramphenicol, and $3.13{\mu}g/ml$ to > $100{\mu}g/ml$ for kanamycin and neomycin. Most of 214 isolates were sensitive to ampicillin and erythromycin, but 20. 1~78. 0% of the strains were resistant to tetracycline, kanamycin, penicillin-G and neomycin. Single or double drug resistance were observed in 75.8% of the resistant strains. The most common resistance patterns were Nm P-G(37.4%) in double pattern and P-G(23.7%) in single pattern.

• 대한미생물학회지
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• 제15권1호
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• pp.47-54
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• 1980

Exoenzymes, protease(P) and elastase(E) produced by Pseudomonas aeruginosa are reported to have close relationship with pathogenicity of Pseudomonas aeruginosa. Productibility of exoenzymes P and E were studied and compared in environmental isolates from hospital environments and clinical isolates from various clinical specimens, also, the relationship between their enzyme production and serotype were reviewed. 1. Clinical isolates were typed into nine serotypes A, B, C, D, E, F, G, H and I. Serotype E had the highest incidence of 24%, followed by B with 16.8%, G, 15.1% and C, 9.3%. 2. Environmental isolates were, typed as serotype B, C, E, F, G, H, I, K and M. Serotype I had the highest incidence of 26.6%, followed by C, F and M each incidence of 14.3%. 3. In the typing of the above two groups, serotypes A and D were found only in the clinical isolates and serotypes K, and M were found only in the environmental isolates. Serotypes J and L were found in neither clinical isolates nor environmental isolates. 4. In the distribution of serotypes from various clinical specimens, serotype G among isolates from pus showed incidence of 20.4%, and serotypes E and B were 19.5% separately. Serotype E had incidence of 22.6% and 20.0% in urine and sputa respectively, showing a high rate compared to the other serotypes. 5. The incidence of strains producing both exoenzymes P and E was 77.8% in the preserved strains of clinical isolates and 76.2% in the environmental isolates. There were no significant difference between the two groups. 6. Serotypes A and H, which are preserved strains from clinical isolates showed productibility of both exoenzymes P and E, the other serotypes showed productibility of various combination of exoenzymes. Among the environmental isoaltes, production of both exoenzymes P and E were seen in serotypes E, F, G, H, I and K and no serotype produced only P or E. 7. In ability to produce exoenzymes of isolates from sources of various clinical specimens, strains producing both exoenzymes P and E were found most frequently in pus with incidence rate of 82.0%, followed by 80.0% in sputum and urine. 8. Almost all the fresh strains of clinical isolates were producers of both exoenzymes P and E.

• 대한수의학회지
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• 제35권2호
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• pp.297-306
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• 1995

The present study was conducted to investigate the incidence of Streptococcus suis infection in the pneumonic lungs of slaughter pigs from December 1992 to April 1994 and to examine the biochemical and cultural properties, the antimicrobial susceptibility and serotype of the isolates. Fifty-six(7.95%) strains of S suis was isolated from 704 pneumonic lungs of slaughter pigs, of which 33 cultures were isolated alone, while remaining 18 were complicated wide Pasteurella multocida. Of the 56 S suis isolates, 18(32.1%) were serotype 2, 5(8.9%) were serotype 1/2, 2 were serotype 1, 1 each for serotype 3 & 5, and the remaining 29 were untypable. The twenty five strains(44.6%) of all isolates produced hyaluronidase while 15 strains(88.3%) of 18 serotype 2 isolates were hyaluronidase producers. All of isolates were highly susceptible to ampicillin, amoxicillin, ceftiofur, cephalothin, ciprofloxacin and penicillin G, although the majority of them were resistant to kanamycin, colistin, erythromycin, oxytetracycline.

• 대한미생물학회지
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• 제35권2호
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• pp.171-180
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• 2000

Ninety two strains of Streptococcus pyogenes were isolated from patients with pharyngitis, scarlet fever, skin infection, and invasive streptococcal infections in Seoul, Korea from January to December, 1998. All isolates were epidemiologically characterized by T protein serotype, and serum opacity factor (OF) detection to phenotypes. To analyze the genetic relationship, fifty two isolates including 32 erythromycin-clindamycin (Em-Cm) resistant strains, 20 antimicrobial susceptible strains were attempted to the pulsed-field gel electrophoresis (PFGE). T protein serotype showed 16 kinds in distribution including T12 and T4. Among the total isolates, 40 strains (43.5%) belonged to the T12 serotype and twenty strains (21.7%) to T4 serotype. On the other hand, when infection aspect of S. pyogenes isolates were analysed by T serotype distribution, T12 type was predominant for pharyngitidis which contributed to 21 strains (53%) and for skin infection isolates which contributed to 11 strains (28%), respectively. In case of T4 type, it was the most predominant pharyngitidis isolates which contributed to 8 strains (40%). In T serotype distribution of Em-Cm resistant strains, 27 strains (84%) of the thirty two showed T12 serotype. In minimum inhibitory concentration (MIC) values of Em-Cm resistance isolates, thirty two isolates showed resistant to erythromycin 27 strains (84%), had high MIC of >$128\;{\mu}g/ml$. And also to clindamycin, twenty two strains (69%) had high MIC of >$128\;{\mu}g/ml$. When OF detection of Em-Cm resistance of S. pyogenes isolates were analyzed by T serotype distribution, T12 serotype isolates revealed that all of the isolates except one strain were OF negative. In PFGE profile analysis to Em-Cm resistance isolates, of the twenty seven, Em-Cm resistance of T12 serotype isolates, 26 strains showed identical PFGE profile and all of these isolates revealed that OF negative. Eighty four percent of Em-Cm resistance S. pyogenes isolates had identical phenotype and PFGE profile. These results strongly suggested that the Em-Cm resistant S. pyogenes isolates from Seoul area showed close genetic correlation and PFGE could be available tool for molecular epidemiology.

• 대한미생물학회지
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• 제18권1호
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• pp.23-29
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• 1983

An epidemiological survey was carried out to identify the serotypes of Streplococcus mutans isolated from carious lesions of 65 caries-active subjects(CAS) and sound tooth surfaces of 40 caries-free subjects(CFS). The autoclaved antigen extract was performed on each isolate, and then, serotypes of the isolates were determined in agar-gel immunodiffusion test. The results were as follows: 1. S. mutans was found in 78% of the samples of CAS, and of CFS. The difference of isolation frequency between CAS and CFS was not observed. 2. Only one serotype per single subject was detected in 61% of total samples, in remaining 39% of samples two or more serotypes were detected. 3. In 41.2% of CAS samples plural serotypes of S. mutans were found, whereas 35.5% of CFS samples showed plural serotypes distribution. 4. The most frequently identified serotype in each subject was serotype c; 69.5% of subjects harbored serotype c S. mutans. Serotype d was next most frequently isolated from subjects, comprising 23.2%. 5. Serotype c strain was found in 64.7% of CAS, 77.4% of CFS. 6. Of the isolates from CAS and CFS, serotype c was most commonly found, comprising 48.8%, serotype d was found in 16.3%, serotypes f. e, and g comprising 13.2%, 9.3%, and 7.8% respectively. Serotypes a and b were also found but in far lower frequencies(2.3%, 0.8%). 7. Serotype c strains were more found in CFS than in CAS, but serotypes d and e were more found in CAS.