• 비교문화연구
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• 제32권
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• pp.109-132
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• 2013

This paper compares sequential and non-sequential verbal conjunctions in Korean, English, and Japanese by looking at how sequential verbal conjunction is treated in each language. It frist reviews verbal conjunctions in Korean, where sequential conjunction is treated as subordination and non-sequential conjunction is treated as coordination, and looks at verbal conjunctions in English and Japanese to see whether or not sequential conjunction in those languages is subordination. According to Oh (2010), sequential and non-sequential conjunctions in Korean behave quite differently with respect to the tense and negation in the final conjunct. Also, Cho (1995, 2005) and Kwon (2004) show that syntactic operations such as extraction and scrambling clearly distinguish sequential conjunction from non-sequential conjunction. The purpose of this paper is to see how sequential and non-sequential conjunctions are analyzed in English and Japanese and to compare those languages with Korean, especially focusing on whether or not sequential conjunctions in English and Japanese are treated as subordination. For this purpose, I first investigate how tense and negation, which provided crucial evidence for concluding that Korean sequential conjunction is subordination, is interpreted in sequential and non-sequential verbal conjunctions in English and Japanese. Also, I investigate the syntactic properties of sequential and non-sequential conjunctions with respect to syntactic operations such as extraction and scrambling in those languages. The results of the investigation show that in Japanese, which is considered typologically similar to Korean, the sequential conjunction is a case of subordination, while in English, which is considered typologically different from Korean, both sequential and non-sequential conjunctions are treated as coordination.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.797-802
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• 2001

Two strains of rumen fungi (Piromyces rhizinflata B157, Orpinomyces joyonii SG4) and three strains of rumen cellulolytic bacteria (Ruminococcus albus B199, Ruminococcus flavefaciens FD1 and Fibrobacter succinogenes S85) were used as mono-cultures or combinationally arranged as co- and sequential-cultures to assess the relative contributions and interactions between rumen fungi and cellulolytic bacteria on rice straw degradation. The rates of dry matter degradation of co-cultures were similar to those of corresponding bacterial mono-cultures. Compared to corresponding sequential-cultures, the degradation of rice straw was reduced in all co-cultures (P<0.01). Regardless of the microbial species, the cellulolytic bacteria seemed to inhibit the degradation of rice straw by rumen fungi. The high efficiency of fungal cellulolysis seems to affect bacterial degradation rates.

• International Journal of Advanced Culture Technology
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• 제8권2호
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• pp.231-238
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• 2020

Generally, sequential data refers to data having continuity. Text data, which is a representative type of unstructured data, is also sequential data in that it is necessary to know the meaning of the preceding word in order to know the meaning of the following word or context. So far, many techniques for analyzing sequential data such as text data have been proposed. In this paper, four methods of 1d-CNN, LSTM, BiLSTM, and C-LSTM are introduced, focusing on neural network techniques. In addition, by using this, IMDb movie review data was classified into two classes to compare the performance of the techniques in terms of accuracy and analysis time.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1152-1155
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• 2010

In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.

• 대한생식의학회:학술대회논문집
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• 대한생식의학회 2007년도 제52차 춘계학술대회 초록집
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• pp.139-139
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• 2007

• 한국해양바이오학회지
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• 제16권1호
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• pp.36-44
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• 2024

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO₂. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO₂ fixation by increasing the availability of CO₂(HCO₃^-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m²/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO₂ availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO₂ fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

• 한국미생물·생명공학회지
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• 제48권1호
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• pp.1-11
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• 2020

The aim of this study was to investigate the effects of Torulaspora delbrueckii and Saccharomyces cerevisiae, as pure fermenters and mixed fermenters (simultaneous and sequential culture), on the production of non-volatiles and volatiles, and on the antioxidant activity in Golden Dried Longan juice and Golden Dried Longan wines. Alanine, arginine, glutamic acid, leucine, proline, and gamma-aminobutyric acid (GABA) were the most prominent amino acids that were found in these wines. The Golden Dried Longan Wine fermented with monocultures of S. cerevisiae and T. delbrueckii produced a total volatile aroma content of 393.21 mg/l and 383.20 mg/l, respectively. Simultaneous culture of the two organisms produced the highest total volatile aroma content, that affected most volatile compounds including isobutanol, ethyl acetate, ethyl decanoate, ethyl heptanoate, ethyl hexanoate, ethyl pentanoate, isoamyl acetate, and isobutyl acetate. Of the four treatments, the sequential culture possessed the highest total phenolic content (5.80 mg gallic acid equivalents (GAE)/ml). In addition, the total phenolic content significantly correlated with the antioxidant activity of the Golden Dried Juice and Golden Dried Longan Wine. These results suggest that co-cultures of the two organisms used in the production of the Golden Dried Longan Wine may improve the intensity and complexity of its aroma.

• Reproductive and Developmental Biology
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• 제32권3호
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• pp.175-181
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• 2008

This study was conducted to investigate an effective recipient oocyte and culture system for producing of Hanwoo (Korean native cattle) somatic cell nuclear transfer (SCNT) embryos. Hanwoo ear skin fibroblasts were used as donor cells. In vitro matured Hanwoo or Holstein oocytes were enucleated, and single donor cells were transferred into the perivitelline space of the enucleated oocytes. The couplets were subsequently fused and activated. The reconstructed embryos were cultured in a conventional or sequential culture system. In the former, embryos were cultured in CR2aa medium for eight days; in the latter, embryos were cultured in modified CR2aa-A (mCR2-A) for three days and then further cultured in modified CR2aa-B (mCR2-B) for five days. In the experiment with the recipient oocyte, the rate of embryo development to the blastocyst stage was significantly (p<0.05) higher in Hanwoo recipient oocytes than in Holstein ones (48.8% vs 38.9%). BIastocysts derived from Hanwoo recipient oocytes contained significantly (p<0.05) higher numbers of total cells than those derived from Holstein recipient oocytes ($156.0{\pm}68.2$ vs $134.7{\pm}54.8$). There was no difference in the mean proportion of apoptotic cells in blastocysts between the sources of recipient oocytes. In the experiment with the embryo culture system, the blastocyst rate was somewhat higher in sequential system than in conventional system (50.0% vs 43.5%), though there was no significant difference. The numbers of total ($160.0{\pm}69.0$ vs $156.7{\pm}68.4$) and apoptotic cells ($14.0{\pm}10.4$ vs $11.8{\pm}6.4$) were not different between the culture systems. In conclusion, the present study demonstrated that Hanwoo recipient oocytes and the sequential culture system were more effective in supporting the production of Hanwoo SCNT embryos.

• 한국심리학회지 : 코칭
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• 제7권2호
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• pp.1-20
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• 2023

본 연구의 목적은 혁신지향 조직문화가 조직구성원의 혁신행동에 미치는 영향을 알아보고, 그 관계에서 상사의 코칭리더십과 조직원의 기본심리욕구의 순차적 매개효과를 확인하는 데 있다. 이를 위해 6개월 이상 동일한 상사와 근무하고 있는 직장인을 대상으로 온라인 조사를 실시하여 377명분의 자료를 수집하였다. 수집한 자료는 Hayes의 SPSS PROCESS Macro를 활용하여 분석하였다. 본 연구의 결과는 다음과 같다. 첫째, 조직문화와 혁신행동 간 관계가 정적으로 유의함이 확인되었다. 둘째, 조직문화와 혁신행동의 관계에서 코칭리더십과 기본심리욕구의 순차적으로 매개함을 확인하였다. 즉, 혁신지향 조직문화가 혁신행동에 바로 영향을 미친다기보다는, 상사의 코칭리더십 발휘로 이어지고, 코칭리더십이 구성원의 기본심리욕구의 충족에 영향을 미침으로써 더 많은 혁신행동을 촉진한다는 것이다. 이는 조직문화와 상사의 리더십인 환경적 변인이 어떻게 개인의 동기적 변인인 욕구에 영향을 미쳐 개인의 행동적 변인에 어떻게 영향을 주는지에 대한 기초 연구로서 의미가 있다. 이외에 연구 결과를 토대로 학문적 시사점 및 실무적 시사점을 논하였고, 본 연구의 한계 및 후속 연구에 대한 제언을 논하였다.

• 생명과학회지
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• 제21권3호
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• pp.335-340
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• 2011

포도당과 자일로스는 자연계에서 가장 풍부한 물질이며 이들은 효모에 의해 에탄올로 전환될 수 있다. 본 연구에서는 Saccharomyces cerevisiae와 Pichia stipitis을 이용하여 분리배양, 공동배양 그리고 순차배양 등의 조합을 통해 가장 효과적인 발효의 방법을 찾고자 하였다. 분리배양에서 S. cerevisiae은 29.4 g/l의 포도당을 발효하여 14.5 g/l의 에탄올을 생산한 반면에 자일로스를 이용하지 못했다. 그렇지만 P. stipitis은 포도당뿐만 아니라 자일로스도 분해하여 각각 포도당 29.4 g/l로부터 11.9 g/l의 에탄올을, 자일로스 29.0 g/l로부터 11.6 g/l의 에탄올을 생산하였다. 포도당과 자일로스 혼합배양에서, S. cerevisiae은 13.4 g/l의 에탄올을 생산한 반면에 P. stipitis은 21.1 g/l의 에탄올을 생산하였다. 공동배양과 순차배양에서, 공동배양이 18.6 g/l, 순차배양이 12.4 g/l의 에탄올을 생산하여 공동배양이 더 효과적인 것으로 나타났다. 두 효모의 생장에서 영양분의 효과를 보기 위해 yeast nitrogen base (YNB)을 S. cerevisiae가 포도당을 소모한 시점에 첨가하니 자일로스의 소비량과 미생물의 성장이 증가하였고 54.6 g/l의 당 혼합배양액에서 22.5 g/l의 에탄올을 생산하여 0.41 g/g의 수득율을 나타내었다.