• 한국항공운항학회지
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• 제23권4호
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• pp.42-48
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• 2015

This paper has its purpose on constructing a prediction model of the arrival sequencing strategy which reflects the actual sequencing patterns of air traffic controllers. As the first step, we analyzed a pair-wise sequencing of two aircraft entering TMA from different entering points. Based on the historical trajectory data, several traffic factors such as time, speed and traffic density were examined for the model. With statistically significant factors, we constructed a prediction model of arrival sequencing through a binary logistic regression analysis. With the estimated coefficients, the performance of the model was conducted through a cross validation.

• 한국항공운항학회지
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• 제22권3호
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• pp.8-14
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• 2014

Arrival management is a tool which provides efficient flow of traffic and reduces ATC workload by determining aircraft's sequence and schedules while they are in cruise phase. As a decision support tool, arrival management should advise on air traffic control service based on the understanding of human factor of its user, air traffic controller. This paper proposed a prediction model for air traffic controller sequencing strategy by analyzing the historical trajectory data. Statistical analysis is used to find how air traffic controller decides the sequence of aircraft based on the speed difference and the airspace entering time difference of aircraft. Logistic regression was applied for the proposed model and its performance was demonstrated through the comparison of the real operational data.

• Fisheries and Aquatic Sciences
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• 제9권2호
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• pp.70-74
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• 2006

DNA sequence-based typing is considered a robust tool for the discrimination of dinoflagellate species because of the availability of extensive rDNA sequences. Here, we present a rapid, cost-effective DNA-sequencing technique for various PCR products. This sequencing strategy relies on 'nested' or 'tailed' primer labeled with near-infrared dye, and uses a minimal volume of unpurified PCR product (ca. $5{\mu}L$) as the DNA template for sequencing reactions. Reliable and accurate base identification was obtained for several hundred PCR fragments of rRNA genes. This quick, inexpensive technique is widely applicable to sequence-based typing in clinical applications, as well as to large-scale DNA sequencing of the same genomic regions from related species for studies of molecular evolution.

• 한국정보시스템학회지:정보시스템연구
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• 제25권1호
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• pp.159-182
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• 2016

Purpose This paper aims to develop a novel tabu search algorithm for solving the sequencing problems with precedence constraints. Due to constraints, the traditional meta heuristic methods can generate infeasible solutions during search procedure, which must be carefully dealt with. On the contrary, the candidate order based tabu search (COTS) is based on a novel neighborhood structure that guarantees the feasibility of solutions, and can dealt with a wide range of sequencing problems in flexible manner. Design/methodology/approach Candidate order scheme is a strategy for constructing a feasible sequence by iteratively appending an item at a time, and it has been successfully applied to genetic algorithm. The primary benefit of the candidate order scheme is that it can effectively deal with the additional constraints of sequencing problems and always generates the feasible solutions. In this paper, the candidate order scheme is used to design the neighborhood structure, tabu list and diversification operation of tabu search. Findings The COTS has been applied to the single machine job sequencing problems, and we can see that COTS can find the good solutions whether additional constraints exist or not. Especially, the experiment results reveal that the COTS is a promising approach for solving the sequencing problems with precedence constraints. In addition, the operations of COTS are intuitive and easy to understand, and it is expected that this paper will provide useful insights into the sequencing problems to the practitioners.

• 한국환경교육학회지:환경교육
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• 제17권1호
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• pp.133-143
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• 2004

This study aims to explore the ways in spreading and maturing of environmental Education(EE) in secondary school. To deal with this issue, researchers investigate the actual conditions of EE and grope for the ways in spreading and maturing of EE. According to investigation, the selecting rate of environment subject in secondary school is about 3%(compared selecting rate of other subject matters) in 2003. Based on the result, this study suggests followings. First, sequencing strategy of EE curriculum will be expected very efficaciously. Secondly, there is need to empasize daily experiences of EE beyond the formal subject matter. Thirdly and ultimately, thers is need to reform school curriculum centered EE. Moreover, there is need to empasize teacher education for the environment not only pre service but also in-service teacher training.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.153-165
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• 2010

Brassica rapa is considered an ideal candidate to act as a reference species for Brassica genomic studies. Among the three basic Brassica species, B. rapa (AA genome) has the smallest genome (529 Mbp), compared to B. nigra (BB genome, 632 Mbp) and B. oleracea (CC genome, 696 Mbp). There is also a large collection of available cultivars of B. rapa, as well as a broad array of B. rapa genomic resources available. Under international consensus, various genomic studies on B. rapa have been conducted, including the construction of a physical map based on 22.5X genome coverage, end sequencing of 146,000 BACs, sequencing of >150,000 expressed sequence tags, and successful phase 2 shotgun sequencing of 589 euchromatic region-tiling BACs based on comparative positioning with the Arabidopsis genome. These sequenced BACs mapped onto the B. rapa genome provide beginning points for genome sequencing of each chromosome. Applying this strategy, all of the 10 chromosomes of B. rapa have been assigned to the sequencing centers in seven countries, Korea, UK, China, India, Canada, Australia, and Japan. The two longest chromosomes, A3 and A9, have been sequenced except for several gaps, by NAAS in Korea. Meanwhile a China group, including IVF and BGI, performed whole genome sequencing with Illumina system. These Sanger and NGS sequence data will be integrated to assemble a draft sequence of B. rapa. The imminent B. rapa genome sequence offers novel insights into the organization and evolution of the Brassica genome. In parallel, the transfer of knowledge from B. rapa to other Brassica crops would be expected.

• 한국경영과학회지
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• 제37권3호
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• pp.39-55
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• 2012

This paper presents an endosymbiotic evolutionary algorithm (EEA) to solve both problems of line balancing and model sequencing in a mixed-model two-sided assembly line (MMtAL) simultaneously. It is important to have a proper balancing and model sequencing for an efficient operation of MMtAL. EEA imitates the natural evolution process of endosymbionts, which is an extension of existing symbiotic evolutionary algorithms. It provides a proper balance between parallel search with the separated individuals representing partial solutions and integrated search with endosymbionts representing entire solutions. The strategy of localized coevolution and the concept of steady-state genetic algorithms are used to improve the search efficiency. The experimental results reveal that EEA is better than two compared symbiotic evolutionary algorithms as well as a traditional genetic algorithm in solution quality.

• Genomics & Informatics
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• 제16권4호
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• pp.30.1-30.6
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• 2018

There is urgent need for effective and cost-efficient data storage, as the worldwide requirement for data storage is rapidly growing. DNA has introduced a new tool for storing digital information. Recent studies have successfully stored digital information, such as text and gif animation. Previous studies tackled technical hurdles due to errors from DNA synthesis and sequencing. Studies also have focused on a strategy that makes use of 100-150-bp read sizes in both synthesis and sequencing. In this paper, we a suggest novel data encoding/decoding scheme that makes use of long-read DNA (~1,000 bp). This enables accurate recovery of stored digital information with a smaller number of reads than the previous approach. Also, this approach reduces sequencing time.

• 상하수도학회지
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• 제32권1호
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• pp.11-18
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• 2018

As the sequencing batch reactor process is a time-oriented system, it has advantages of the flexibility in operation for the biological nutrient removal. Because the sequencing batch reactor is operated in a batch system, respiration rate is more sensitive and obvious than in a continuous system. The variation of respiration rate in the process well represented the characteristics of biological reactions, especially nitrification. The respiration rate dropped rapidly and greatly with the completion of nitrification, and the maximum respiration rate of nitrification showed the activity of nitrifiers. This study suggested a strategy to control the aeration of the sequencing batch reactor based on respirometry. Aeration time of the optimal aerobic period required for nitrification was daily adjusted according to the dynamics of respiration rate. The aeration time was mainly correlated with influent nitrogen loadings. The anoxic period was extended through aeration control facilitating a longer endogenous denitrification reaction time. By respirometric aeration control in the sequencing batch reactor, energy saving and process performance improvement could be achieved.

• Journal of Genetic Medicine
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• 제7권2호
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• pp.119-124
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• 2010

약 7,000 여개의 단일유전자질환이 보고되어 있지만 보고된 질환의 절반도 아직 원인 유전자가 밝혀지지 못한 상황이다. 그리고 기존에 밝혀진 원인 유전자의 돌연변이형들은 대부분 단백질을 코딩하는 부위의 돌연변이에 의하여 발생하고 있어서 인간 유전체에서 단백질을 코딩하는 엑손 부위만을 선별적으로 분리하여 염기서열을 분석하는 엑솜 염기서열 분석 방법은 희귀한 유전질환의 신규 원인 유전자 발굴을 위한 매우 효과적인 유전 분석법이 될 것이다. 엑솜은 전체 유전체의 약 1.5% 정도를 차지하고 있어서 매우 경제적으로 분석이 가능하다. 그리고 엑솜 염기서열 분석 방법은 엑솜 부위를 선별하는 기술과 대용량 염기서열 분석기술로 수행된다. Freeman-Sheldon 증후군의 원인 유전자를 엑솜 염기서열 분석 방법으로 발굴한 이후로 단일유전자질환의 원인 유전자 발굴을 위한 표준 분석법으로 엑솜 염기서열 분석방법이 사용되고 있다. 향후에는 엑솜 염기서열 분석 방법이 다양한 복합질병의 유전분석에도 활용되어 개인 맞춤의학의 실현을 앞당기는데 크게 기여할 것으로 기대된다.