• Journal of Microbiology and Biotechnology
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• v.8 no.3
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• pp.284-286
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• 1998

A ura5 gene encoding Orotate Phosphoribosyl Transferase (OPRTase) of Metarhizium anisopliae was cloned by PCR methods and sequenced. The sequenced ura 5 gene encodes a polypeptide of 234 amino acid residues. This deduced amino acid sequence showed high similarity to other fungi OPRTase and there was no intron sequence between ATG starting codon and TGA ending codon.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.5
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• pp.889-896
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• 2007

Advances in wireless networking, micro-fabrication and integration, and embedded microprocessors have enabled a new generation of massive-scale sensor networks. Because each sensor node is limited in size and capacity, it is very important to design a new simple and energy efficient protocol. Among conventional sensor networks' routing protocols, the directed diffusion scheme is widely blown because of its simplicity. This scheme, however, has a defect in that sending interest and exploratory data messages while setting connection paths consumes much energy because of its flooding scheme. Therefore, this paper proposes a new sensor network routing protocol, called sequenced directed diffusion with a threshold control, which compromises the conventional directed diffusion scheme's defect and offers an energy efficient routing idea. With a computer simulation, its performance is evaluated and compared to the conventional directed diffusion scheme. Numerical results show that the proposed scheme offers energy efficiency while routing packets, and resolves ill-balanced energy consumption among sensor nodes.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.6
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• pp.2781-2800
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• 2016

Emerging attacks aim to access proprietary assets and steal data for business or political motives, such as Operation Aurora and Operation Shady RAT. Skilled Intruders would likely remove their traces on targeted hosts, but their network movements, which are continuously recorded by network devices, cannot be easily eliminated by themselves. However, without complete knowledge about both inbound/outbound and internal traffic, it is difficult for security team to unveil hidden traces of intruders. In this paper, we propose an autonomous anomaly detection system based on behavior profiling and relation mining. The single-hop access profiling model employ a novel linear grouping algorithm PSOLGA to create behavior profiles for each individual server application discovered automatically in historical flow analysis. Besides that, the double-hop access relation model utilizes in-memory graph to mine time-sequenced access relations between different server applications. Using the behavior profiles and relation rules, this approach is able to detect possible anomalies and violations in real-time detection. Finally, the experimental results demonstrate that the designed models are promising in terms of accuracy and computational efficiency.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.38-43
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• 2016

Novel and specific recognition elements are of central importance in the development of a pathogen detection method. Here, we describe a simple method for identifying the cell-wall binding domain (CBD) from a sequenced bacterial genome employing homology search for phage lysin genes. A putative CBD (CPF369_CBD) was identified from a genome of Clostridium perfringens type strain ATCC 13124, and its function was studied with the CBD-GFP fusion protein recombinantly expressed in Escherichia coli. Fluorescence microscopy showed the specific binding of the fusion protein to C. perfringens cells, which demonstrates the potential of this method for the identification of novel bioprobes for specific detection of pathogenic bacteria.

• English Language & Literature Teaching
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• v.17 no.2
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• pp.39-58
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• 2011

This study highlights some very important future directions for the revision of language forms in the 2007 Korean English Curriculum, expected to be made public in the summer of 2011. A critical review of the 2007 National English Curriculum shows that language forms exemplified in that curriculum are not well-chosen or well-sequenced from the viewpoint of grammar selection and gradation, and that no mention of structural labels or grammatical terminologies makes it hard for teachers to recognize their formal properties. To fulfill the original purpose that languages should be listed in the curriculum so that functional-notional syllabuses can be complemented by form-focused instruction, the study presents partial inventories of grammatical items with well-chosen and well-sequenced examples. Minimal descriptions are given to these inventories that are based on general notions or well-known grammatical notions. Occasional suggestions are also made for which language forms should be taught in which schools.

• Journal of Life Science
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• v.9 no.4
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• pp.341-347
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• 1999

Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-$\beta$-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.

• Animal Systematics, Evolution and Diversity
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• v.26 no.3
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• pp.197-201
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• 2010

We sequenced the whole mitochondrial genome of Dendronephthya gigantea (Anthozoa: Octocorallia: Nephteidae), the first mitochondrial genome sequence report in the Family Nephtheidae. The mitochondrial genome of D. gigantea was 18,842 bp in length, and contained 14 protein coding genes (atp6 and 8, cox1-3, cytb, nd1-6 and 4L, and msh1), two ribosomal RNAs, and only one transfer RNA. The gene content and gene order is identical to other octocorals sequenced to date. The portion of the noncoding regions is slightly larger than the other octocorals (5.08% compared to average 3.98%). We expect that the information of gene content, gene order, codon usage, noncoding region and protein coding gene sequence could be used in the further analysis of anthozoan phylogeny.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.99-103
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• 2014

Mitochondrial genome sequence of malaria parasites has served as a potential marker for inferring evolutionary history of the Plasmodium genus. In Plasmodium falciparum, the mitochondrial genome sequences from around the globe have provided important evolutionary understanding, but no Indian sequence has yet been utilized. We have sequenced the whole mitochondrial genome of a single P. falciparum field isolate from India using novel primers and compared with the 3D7 reference sequence and 1 previously reported Indian sequence. While the 2 Indian sequences were highly divergent from each other, the presently sequenced isolate was highly similar to the reference 3D7 strain.

• The Korean Journal of Mycology
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• v.48 no.2
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• pp.135-149
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• 2020

Da-Lat is a hilly area located in southern Vietnam. Macrofungal diversity of Da-Lat was investigated from 2018 to 2019. A total of 468 macrofungal specimens was collected and identified using the modern species concept and taxonomic and phylogenetic analyses. Among them, internal transcribed spacer(ITS) region of 401 specimens were successfully sequenced and compared with those of related species retrieved from GenBank. In total, 180 specimens were identified at the species level. The sequenced specimens were classified into 2 phyla, 13 orders, 38 families, 93 genera, and 124 species. The remaining 221 specimens (175 species) did not match the species level. This study is the first well-documented taxonomic list of macrofungi collected from southern Vietnam.

• Journal of Microbiology
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• v.33 no.4
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• pp.350-354
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• 1995

Saccharomyces cervisiae has a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. Mutants defective in any one of the our cell division cycle genes (CDC3, CDC10, CDC11, CDC12) fail to form these filaments and exhibit a pleiotropic phenotype that includes failure to complete cytokinesis and abnormal bud growth. However, the role of the filament is not clear. In order to find out the role of filament, the similar gene in S pombe (called cdc103$\^$+) to the CDC3 was cloned and sequenced. Here I report the sequence analysis of the cdc103$\^$+/ ) to the CDC3 was cloned and sequenced. Here I report the sequence analysis of the cdc103$\^$+/. Comparison of the predicted amino acid sequences of cdc103$\^$+/ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other.