• 제목/요약/키워드: Sequence-pair

검색결과 310건 처리시간 0.028초

The complete chloroplast genome sequence of Avena sterilis L. using Illumina sequencing

  • Raveendar, Sebastin;Lee, Gi-An;Lee, Kyung Jun;Shin, Myoung-Jae;Cho, Yang-Hee;Ma, Kyung-Ho;Chung, Jong-Wook;Lee, Jung-Ro
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.139-139
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    • 2017
  • The complete chloroplast genome sequence of Avena sterilis L., a dominant wild oat species in the family Poaceae, is first reported in this study. The complete cp genome sequence of A. sterilis is 135,887 bp in length with 38.5% overall GC content and exhibits a typical quadripartite structure comprising one pair of inverted repeats (21, 603 bp) separated by a small single-copy region (12,575 bp) and a large single-copy region (80,106). The A. sterilis cp genome encodes 111 unique genes, 76 of which are protein-coding genes, 4 rRNA genes, 30 tRNA genes and 18 duplicated genes in the inverted repeat region. Nine genes contain one or two introns. Pair-wise alignments of cp genome were performed for genome-wide comparison. This newly determined cp genome sequence of A. sterilis will provide valuable information for the future breeding programs of valuable cereal crops in the family Poaceae.

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사람의 ε-글로빈 프로모트에서 d(CXG)와 d(GXC)의 안정성에 인접한 염기 서열들의 영향 에 관한 연구 (Effect of Neighbor Base Sequences on the Base Pair Stabilities at d(CXG) and d(GXC) in Human ε-globin Promoter)

  • 정인애;강종백
    • 생명과학회지
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    • 제12권2호
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    • pp.208-212
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    • 2002
  • 온도 기울기 전기영동장치를 이용하여 d(CXG)와 d(GXC) 염기의 열 안정성을 결정하는데 사람의 $\varepsilon$-글로빈 DNA조각을 사용하였다. 염기 쌍의 안정성은 이웃하는 염기서열에 의한 수소결합과 base stocking 상호작용에 의존한다. 염기 쌍의 안정성은 d(CXG) d(CYG)의 경우에 T.AG.A = A.G>C.T>T.C>C.A>A.C이다.

Syllable-timing Interferes with Korean Learners' Speech of Stress-timed English

  • Lee, Ok-Hwa;Kim, Jong-Mi
    • 음성과학
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    • 제12권4호
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    • pp.95-112
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    • 2005
  • We investigate Korean learners' speech-timing of English before and after instruction in comparison with native speech, in an attempt to resolve disagreements in the literature as to whether speech-timing is measurable (Lehiste, 1977; Roach, 1982; Dauer, 1983 vs. Low et al., 2000; Yun 2002; Jian, 2004). We measured the pair-wise variability between the adjacent stressed and unstressed syllables within a foot as well as that among adjacent feet in approximately 555 English sentences, which were read by 29 native speakers and 41 Korean learners in the intermediate proficiency level. The results show that in comparison with native American English, Korean learner speech is before instruction significantly (p<.001) smaller for the pair-wise variability between the adjacent stressed and unstressed syllables within a foot; and significantly (p=.01) bigger for the variability among adjacent feet within the utterance. The learner speech after instruction showed significant (p=.01) improvement in the pair-wise variability of syllable sequence toward native speech values. The variability among adjacent feet was progressively smaller for learner speech before and after instruction and for native speech (p=.03). We thus conclude that the speech timing difference between Korean English and American English is measurable in terms of the duration. of stressed and unstressed syllables and that the latter is stress-timed and the former is syllable-timing interfered.

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능이버섯의 ITS염기서열과 유전적 변이 (The Base Sequence of ITS and Genetic Variation in Sarcodon Aspratus)

  • 김종봉
    • 생명과학회지
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    • 제14권6호
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    • pp.963-966
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    • 2004
  • 능이버섯의 16S ribosomal DNA일부분, IT1, 5.85 ribosomal DNA, ITS2의 전부분, 28S ribosomal DNA 일부분이 포함된 ITS영역의 염기서열을 분석하였다. 이 부분은 716개의 염기쌍으로 구성되었다. 이를 Sarcadon속에 속하는 종들과 비교분석한 결과 같은 능이버섯의 ITS에 관한 다른 분석결과 염기치환 및 결실을 근거로 하였을 경우 $1.8\%$의 차이를 나타내었다. 또한 S. imbricatus와는 $1.8\%$, S. sequamous와는 $10\%$차이를 나타내었다. 이는 능이버섯이 숙주, 서식지 환경 등의 특수성 때문에 자연상태에서 유전자교류가 일어나지 않기 때문인 것으로 생각된다.

Correlation Analysis between Regulatory Sequence Motifs and Expression Profiles by Kernel CCA

  • Rhee, Je-Keun;Joung, Je-Gun;Chang, Jeong-Ho;Zhang, Byoung-Tak
    • 한국생물정보학회:학술대회논문집
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    • 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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    • pp.63-68
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    • 2005
  • Transcription factors regulate gene expression by binding to gene upstream region. Each transcription factor has the specific binding site in promoter region. So the analysis of gene upstream sequence is necessary for understanding regulatory mechanism of genes, under a plausible idea that assumption that DNA sequence motif profiles are closely related to gene expression behaviors of the corresponding genes. Here, we present an effective approach to the analysis of the relation between gene expression profiles and gene upstream sequences on the basis of kernel canonical correlation analysis (kernel CCA). Kernel CCA is a useful method for finding relationships underlying between two different data sets. In the application to a yeast cell cycle data set, it is shown that gene upstream sequence profile is closely related to gene expression patterns in terms of canonical correlation scores. By the further analysis of the contributing values or weights of sequence motifs in the construction of a pair of sequence motif profiles and expression profiles, we show that the proposed method can identify significant DNA sequence motifs involved with some specific gene expression patterns, including some well known motifs and those putative, in the process of the yeast cell cycle.

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Photocleavage of DNA by 4'-Bromoacetophenone Analogs

  • Jeon, Ra-Ok;Pual A.Wender
    • Archives of Pharmacal Research
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    • 제24권1호
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    • pp.39-43
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    • 2001
  • 4'-Bromoacetophenone analogs, which are able to generate monophenyl radicals capable of hydrogen atom abstraction, were investigated as possible photoinducible DNA cleaving agents. The potential of 4'-Bromoacetophenone as a possible new DNA cleaver is explored. Pyrrolecarboxatmid conjugated 4'-Bromoacetophenone, in particular, DNA cleaving activity and sequence-selectivity on the contiguous AT base pair sites.

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동축 선로에서의 초고속 근거리 전송을 위한 FEXT 제거기 (FEXT cancellation for high-speed local transmission over twisted pair wiring)

  • 우정수;강규민;임기홍
    • 한국통신학회논문지
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    • 제27권8C호
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    • pp.782-791
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    • 2002
  • 본 논문은 FEXT (far-end crosstalk) canceller에 관한 연구이다. Fiber to the curb (FTTC), very high speed digital subscriber와 같은 많은 동축선로 시스템에서 양방향 전송을 위해 frequency-division duplexing (FDD)를 사용한다. FDD 동축전송 시스템의 최고의 도달 거리는 하향채널보다는 높은 주파수에 위치한 상향 채널에 의해서 제한된다. 그런 FDD 송수신의 성능을 개선하기 위해서 높은 주파수에 있는 채널은 FEXT 제거를 적용하게 된다. 시스템을 배열과 그것은 blind startup 은 채널의 intersymbol interference (ISI), FEXT와 다른 잡음을 막기 위해 FEXT 제거기와 등화기를 함께 적용함으로써 연구한다. 이상적 훈련 신호를 전송하지 않고, 제안된 동축선로상에서 초기 수렴과 수렴된 후의 상태를 알아본다. 또한 FEXT 채널의 분석한 모델뿐만 아니라, 측정된 특성은 FEXT 제거기를 위해 필요한 기간을 측정하기 위해 사용되어진다. 또한 FEXT 제거기를 위한 메모리 수가 채널에 독립적이기 때문에, 제안된 시스템은 모든 독립적인 채널 상에서 동축 시스템에 대해 유용하게 적용할 수 있다.

Bacillus stearothermophilus $\beta$-Xylosidase 유전자의 염기 서열 결정 및 분석 (Sequence Analysis of $\beta$-Xylosidase Gene from Bacillus stearothermophilus)

  • 오현주;최용진
    • 한국미생물·생명공학회지
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    • 제22권2호
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    • pp.134-142
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    • 1994
  • The neucleotide sequences of the xylA gene encoding $\beta $-xylosidase of Bacillus stearothermophilus and is its flanking regions were datermined. Three open reading frame(ORFs) were found, one of which(ORF1) appeared to code for the $\beta $-xylosidase. The 1830 base pair ORF1 encoded 609 amino acids starting from a TTG initiation codon. The molecular weight deduced from the nucleotide sequence(68 KD) was in agreement with that estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme(66 KD). The Shine-Dalgarno sequence(5'-AGGAGG-3') was found 11 bp upstream of the initiation codon. Further 15 bp upstream, there observed a potential transcription initiation signals. The putative -10 sequence(CATAAT) and -35 sequence(TTGTTA) coresponded closely to the consensus sequences for Bacillus subtilis RNA polymerase with major sigma factor. The guanine-plus-cytosine content of the coding region of the xylA gene was 56mol% while that of the third position of the codons was 63 mol%. Based on the comparison with the amino acid sequences of several other carbohydrate degrading enzymes, two conserved regions, possibly participating in the catalytic mechamism of $\beta $-xylosidase xylA, were identified in 278-298 and 329-350 regions of the translated xylA gene. The nucleotide sequence of the xylA was found to exhibit no homology to any other genes so far reproted.

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The complete chloroplast genome sequence of Korean Neolitsea sericea (Lauraceae)

  • PARK, Yoo-Jung;CHEON, Kyeong-Sik
    • 식물분류학회지
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    • 제51권3호
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    • pp.332-336
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    • 2021
  • The complete chloroplast (cp) genome sequence of Neolitsea sericea was determined by Illumina sequencing. The complete cp genome was 152,446bp in length, containing a large single-copy region of 93,796 bp and a small single-copy region of 18,506bp, which were separated by a pair of 20,072bp inverted repeats. A total of 112 unique genes were annotated, including 78 protein-coding genes (PCGs), 30 transfer RNAs, and four ribosomal RNAs. Among the PCGs, 18 genes contained one or two introns. A very low level of sequence variation between two cp genomes of N. sericea was found with seven insertions or deletions and only one single nucleotide polymorphism. An analysis using the maximum likelihood method showed that N. sericea was closely related to Actinodaphne trichocarpa.

Complete genome and two plasmids sequences of Lactiplantibacillus plantarum L55 for probiotic potentials

  • Bogun Kim;Kiyeop Kim;Xiaoyue Xu;Hyunju Lee;Duleepa Pathiraja;Dong-June Park;In-Geol Choi;Sejong Oh
    • Journal of Animal Science and Technology
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    • 제65권6호
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    • pp.1341-1344
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    • 2023
  • In this study, we report the complete genome sequence of Lactiplantibacillus plantarum L55, a probiotic strain of lactic acid bacteria isolated from kimchi. The genome consists of one circular chromosome (2,077,416 base pair [bp]) with a guanine cytosine (GC) content of 44.5%, and two circular plasmid sequences (54,267 and 19,592 bp, respectively). We also conducted a comprehensive analysis of the genome, which identified the presence of functional genes, genomic islands, and antibiotic-resistance genes. The genome sequence data presented in this study provide insights into the genetic basis of L. plantarum L55, which could be beneficial for the future development of probiotic applications.