• BMB Reports
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• 제30권1호
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• pp.80-84
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• 1997

The synthesis and secretion of human angiogenin in E. coli by the natural leader sequence has been studied. We constructed a recombinant plasmid containing human angiogenin cDNA which encompassed all the coding region including leader sequence required for secretion. The recombinant plasmid was introduced into a suitable E. coli host. The angiogenin was detected in the culture medium and periplasm upon the induction of gene expression. The molecular weight of the secreted angiogenin was identical to that of authentic angiogenin purfied from human plasma when estimated by SDS-PAGE and immunoblotting. showing that the natural leader sequence was recognized and processed by the secretion machinery of E. coli. The angiogenin concentration in the culture medium reached a maximum within 2 h when expressed at $37^{\circ}C$ with 0.02~2 mM IPTG. In contrast, the expression level increased gradually over time up to 11 h at $23^{\circ}C$ with 0.002~2 mM IPTG and at $37^{\circ}C$ with 0.002 mM IPTG.

• Journal of Electrical Engineering and Technology
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• 제13권6호
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• pp.2319-2328
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• 2018

This paper introduces a control method for a transformerless MMC-HVDC system. The proposed method can effectively control the grid currents of the MMC-HVDC system under unbalanced grid conditions such as a single line-to-ground fault. The proposed method controls the currents of the positive sequence component and the negative sequence component without separating algorithms. Therefore, complicated calculations for extracting the positive sequence and the negative sequence component are not required. In addition, a control method to regulate a zero sequence component current under unbalanced grid conditions in the transformerless MMC-HVDC system is also proposed. The validity of the proposed method is verified through PSCAD/EMTDC simulation.

• 대한전기학회논문지
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• 제40권8호
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• pp.836-844
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• 1991

PLC(programmable logic controller) system is widely used for the control of factory. PLC system receives ladder diagram which is drawn by the user to implement hardware logic, converts the ladder diagram into sequence program which is executable in the PLC system, and executes the sequence program indefinitely unless user breaks. The sequence program processes the data of on/off signal, and endures 1 scan delay and missing of pulse-type signal shorter than a scan time. So, data dependency doesn't exist. By applying theis characteristics to multiprocessor architecture, we design parellel PLC functionally and evaluate performance upgrade. Parallel PLC consists of central processing module, N general processing unit, and a shared memory by master-slave type. Each module executes allocated sequence program by the control of central processing module. We can expect performance upgrade by parallel processing, and reliability by relocation of sequence program when error occurs in processing module.

• Communications for Statistical Applications and Methods
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• 제12권1호
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• pp.139-147
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• 2005

It is interesting to locate homogeneous segments within a DNA sequence. Suppose that the DNA sequence has segments within which the observations follow the same residue frequency distribution, and between which observations have different distributions. In this setting, change points correspond to the end points of these segments. This article explores the use of a binary segmentation procedure in detecting the change points in the DNA sequence. The change points are determined using a sequence of nested hypothesis tests of whether a change point exists. At each test, we compare no change-point model with a single change-point model by using the Bayesian information criterion. Thus, the method circumvents the computational complexity one would normally face in problems with an unknown number of change points. We illustrate the procedure by analyzing the genome of the bacteriophage lambda.

• 한국컴퓨터정보학회논문지
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• 제21권3호
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• pp.57-64
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• 2016

This paper deals with the PCB assembly time minimization problem that the PAP (pick-and-placement) machine pickup the K-weighted group of N-components, loading, and place into the PCB placement location. This problem considers the rotational turret velocity according to component weight group and moving velocity of distance in two component placement locations in PCB. This paper suggest heavy-weight component group first pick-and-place strategy that the feeder sequence fit to the placement location Hamiltonean cycle sequence. This algorithm applies the quadratic assignment problem (QAP) that considers feeder sequence and location sequence, and the linear assignment problem (LAP) that considers only feeder sequence. The proposed algorithm shorten the assembly time than iATMA for QAP, and same result as iATMA that shorten the assembly time than ATMA.

• 대한전자공학회논문지SD
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• 제46권11호
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• pp.24-33
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• 2009

코딩정보의 전송을 위하여 보조선에서 발생하는 오버헤드전이는 Sequence Switching Code (SSC) 알고리즘의 확장성을 제한하는 가장 큰 걸림돌이 되어왔다. 본 논문에서는 보조선을 사용하지 않고 SSC 회로를 구현하는 방법과 함께 이 방법을 사용하여 보조선을 사용하였을 때 보다 오버헤드전이를 적게 발생시키는 방법을 제시하였다. 실험결과 새로운 방법을 적용함으로써 보조선을 사용하는 방식에 비하여 오버헤드전이의 발생을 50% 이하로 줄이고 알고리즘의 효율을 약 30% 향상시킬 수 있었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1988년도 한국자동제어학술회의논문집(국제학술편); 한국전력공사연수원, 서울; 21-22 Oct. 1988
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• pp.943-946
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• 1988

A simple method is proposed for determining the frequency response function G(j.omega.) of a system using a pair of characteristic M-sequences (maximum length linear feed back shift register sequence). When a characteristic M-sequence is sampled with q$_{1}$ and q$_{2}$ both of which are coprime with N, where N is the period of the M-sequence, the obtained pair of sequences have conjugate complex frequency spectrum. Making use of this fact, two charcteristic M-sequences having conjugate complex frequency spectrum are applied to a system to be measured. Since the magnitude of spectrium of M-sequence is known, the gain of G(j.omega.) is directly obtained from the Fourier transform of the system output. The phase of G(j.omega.) is obtained simply by taking the average of the two phases of output spectrum.

• ETRI Journal
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• 제28권1호
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• pp.95-98
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• 2006

In this letter, a novel M-ary code-selected direct sequence (DS) ultra-wideband (UWB) communication system is presented. Our purpose is to achieve a high data rate by an M-ary code-selected direct sequence bipolar pulse amplitude modulation (MCSDS-BPAM) scheme. In this system, a particular DS code sequence is selected by the $log_2M$/2 bits from the DS gold code set. This scheme can accomplish both a high data rate without increasing the system bandwidth or changing the pulse shape and improve the BER with an increase of modulation level M even at a lower signal-to-noise ratio (SNR). The receiver signal processing algorithm is given for an MCSDS-BPAM UWB system over an ideal AWGN channel and correlation receivers.

• 천문학회보
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• 제37권1호
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• pp.37.1-37.1
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• 2012

We present mid-IR (MIR) and near-UV (NUV) properties of red sequence galaxies defined by optical color-magnitude relation. We use the Wide-field Infrared Survey Explorer (WISE) preliminary released data matched with the SDSS DR7/GALEX GR6. The red sequence galaxies with little emission lines show a wide spread of MIR (3.4um-12um) colors, implying a variety of MIR excess emission. We focus on the properties of the red sequence galaxies with MIR excess, comparing the properties of post-starburst galaxies to trace how galaxies migrate to the red sequence.

• 대한기계학회논문집
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• 제18권9호
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• pp.2234-2244
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• 1994

A process sequence of multi-operation cold forging for actual application in industry is designed with the rigid-plastic finite element method to form a constant velocity joint housing(CVJ housing). The material flow during the CVJ housing forming is axisymmetric until the final forging process for forming of ball grooves. This study treats the deformation as an axisymmetric case. The main objective of the process sequence design is to obtain preforms which satisfy the design criteria of near-net-shape product requiring less machining after forming. The process sequence design also investigates velocity distributions, effective strain distributions and forging loads, which are useful information in the real process design.