• Economic and Environmental Geology
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• v.18 no.4
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• pp.381-397
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• 1985

A petrological study has been done in the pyeongchang-Jaecheon area which is a northwestern part of the basement of Ogcheon zone for the purpose of comparison of the area to the Nogjeon-Yeongchun area which is the antipodal basement of the zone in the petrological and geotectonical view points. The major units of the area are Precambrian granitic gneissic complex, banded gneiss, linea ted leucocratic gneiss and pegmatitic leucogranitic gneiss in the west, elongated exposure of quartz schist (or partly quartzite) and phyllite, named as Jungdaegal-bong Group correlated to the lower sequence of Joseon Group, in the middle, and limestone and calcic dolomite, Iptanri Formation, correlated to the middle of Joseon Group in the east. Igneous plutons are distributed in the areas of gneissic complex and limestone formation as well as in the Eosangcheon and Daedaeri areas in the southeastern out of the area. Present study reveals that the gneissic complex are the products of granitization to metamorphism of amphibolite facies in the order of above mentioned from the metasediments of schists and calcareous rocks. A notable characteristics of the phyllite of Jungdaegal-bong Group is the presence of syntectonically segregated quartz rods in the forms of lens, swirl or boudinage in evenly distributed in the phyllitic to chloritic matrix. Igneous rocks range in composition from gabbro through diorite, granodiorite, to schistosed and porphyritic granites in stock and dike. The orogenic movement of the Ogcheon zone initiated in the middle Proterozoic time, pre-sedimentation of Ogcheon Group and superposed the granitization in Permian, Jurassic Daebo orogeny with granitic batholiths and stocks, and Cretaceous plutonic intrusion.

• Bulletin of the Korean Chemical Society
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• v.31 no.5
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• pp.1143-1148
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• 2010

We investigated the separation of nitrogen heterocyclic compound (NHC) contained in a model coal tar fraction comprising four kinds of NHC [indole (In), quinoline (Q), iso-quinoline (iQ), quinaldine (Qu)], three kinds of bicyclic aromatic compound (BAC) [1-methylnaphthalene (1MN), 2-methylnaphthalene (2MN), dimethylnaphthalene (DMN) mixture with ten structural isomers (DMNs; regarded as one component)], biphenyl (Bp) and phenyl ether (Pe) by liquid membrane permeation (LMP). A batch-stirred tank was used as the permeation unit. An aqueous solution of saponin and n-hexane were used as the liquid membrane and the outer oil phase, respectively. Yield and selectivity of individual NHC was much larger than that of BAC, Bp and Pe. Increasing the initial mass fraction of the saponin to the membrane solution ($C_{sap,0}$) and the initial volume fraction of O/W emulsion to total liquid in a stirred tank (${\phi}_{OW,0}$) resulted in deteriorating the yield of individual NHC, but increasing the stirring speed (N) resulted in improving the yield of each NHC. With increasing $C_{sap,0}$, the selectivity of each NHC based on DMNs increased. Increasing ${\phi}_{OW,0}$ and N resulted in decreasing the selectivity of individual NHC based on DMNs. At an experimental condition fixed, the sequence of the yield and selectivity in reference to DMNs for each NHC was Q > Qu = iQ > In. Furthermore, we compared LPM method with methanol extraction method in view of the separation efficiency (yield, selectivity) of NHC.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.277-287
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• 2003

Acetolactate synthase (ALS, EC 4.1.3.18; also referred to as acetohydroxy acid synthase) catalyzes the first common step in the biosynthesis of valine, leucine, and isoleucine in microorganisms and plants. Recently X-ray structure of yeast ALS was available. Pair-wise alignment of yeast and tobacco ALS sequences revealed 63% sequence similarity. Using Deep View and automatic modeling on Swiss model server, we have generated reliable models of tobacco ALS based on yeast ALS template with a calculated pair-wise RMSD of 0.86 Angstrom. Functional roles of four residues located on the subunit interface (H142, El43, M350, and R376) were examined by site-directed mutagenesis. Seven mutants were generated and purified, of which three mutants (H142T, M350V, and R376F) were found to be inactivated under various assay conditions. The H142k mutant showed moderately altered kinetic properties. The E143A mutant increased 10-fold in K$_m$ value while other parameters remained unchanged. The M350C mutant was strongly resistant to three tested herbicides, while the R376k mutant can bind with herbicide carder at similar affinity to that of wild type enzyme, as determined by tryptophan quenching study. Except M350V mutant, all other mutants were ate to bind with cofactor FAD. Taken together, it is likely that residues H142 and E143 are located at the active site, while residues M350 and R376 are possibly located at the overlapping region of active site and herbicide binding site of the enzyme. Our data also allows us to hypothesize that the interaction between side chains of residues M350 and R376 are probably essential for the correct conformation of the active site. It remains to be elucidated that, whether the herbicide, upon binding with enzyme, inactivates the enzyme by causing change in the active site allosterically, which is unfavorable for catalytic activity.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.1-1
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• 2003

It is clear that computers will play a key role in the biology of the future. Even now, it is virtually impossible to keep track of the key proteins, their names and associated gene names, physical constants(e.g. binding constants, reaction constants, etc.), and hewn physical and genetic interactions without computational assistance. In this sense, computers act as an auxiliary brain, allowing one to keep track of thousands of complex molecules and their interactions. With the advent of gene expression array technology, many experiments are simply impossible without this computer assistance. In the future, as we seek to integrate the reductionist description of life provided by genomic sequencing into complex and sophisticated models of living systems, computers will play an increasingly important role in both analyzing data and generating experimentally testable hypotheses. The future of bioinformatics is thus being driven by potent technological and scientific forces. On the technological side, new experimental technologies such as microarrays, protein arrays, high-throughput expression and three-dimensional structure determination prove rapidly increasing amounts of detailed experimental information on a genomic scale. On the computational side, faster computers, ubiquitous computing systems, high-speed networks provide a powerful but rapidly changing environment of potentially immense power. The challenges we face are enormous: How do we create stable data resources when both the science and computational technology change rapidly? How do integrate and synthesize information from many disparate subdisciplines, each with their own vocabulary and viewpoint? How do we 'liberate' the scientific literature so that it can be incorporated into electronic resources? How do we take advantage of advances in computing and networking to build the international infrastructure needed to support a complete understanding of biological systems. The seeds to the solutions of these problems exist, at least partially, today. These solutions emphasize ubiquitous high-speed computation, database interoperation, federation, and integration, and the development of research networks that capture scientific knowledge rather than just the ABCs of genomic sequence. 1 will discuss a number of these solutions, with examples from existing resources, as well as area where solutions do not currently exist with a view to defining what bioinformatics and biology will look like in the future.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.12
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• pp.1703-1709
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• 2008

As genetic markers, single nucleotide polymorphisms (SNP) are very appropriate for the development of genetic tests for economic traits in livestock. Several microsatellite markers have been identified as useful markers for the genetic improvement of Hanwoo. Among those markers, ILSTS035 was recently mapped at a similar position with four SNPs (AH1_11, AH1_9, 31465_446, and 12273_165) in a linkage map of EST-based SNP in BAT6. Among the four SNPs, two SNPs (31465_446 and 12273_165) were analyzed using BLAST at the NCBI web site. The sequences including the 12273_165 SNP were identified at the intron region within the LOC534614 gene on the gene sequence map (Bos taurus NCBI Map view, build 3.1). The LOC534614 gene represents a protein similar to myosin heavy chain, fat skeletal muscle, embryonic isoform 1 in the dog, and myosin_1 (Myosin heavy chain D) in Macaca mulatta. In cattle, the myosin heavy chain was associated with muscle development. The phenotypic data for growth and carcass traits in the 415 animals were analyzed by the mixed ANCOVA (analysis of covariance) linear model using PROC GLM module in SAS v9.1. By the genotyping of Hanwoo individuals (n = 415) to evaluate the association of SNP with growth and carcass traits, it was shown that the 12273_165 SNP region within LOC534614 may be a candidate marker for growth. The results of the statistical analyses suggested that the genotype of the 12273_165 SNP significantly affected birth weight, weight of the cattle at 24 months of age, average daily gain and carcass cold weight (p<0.05). Consequently, the 12273_165 SNP polymorphisms at the LOC534614 gene may be associated with growth in Hanwoo, and functional validation of polymorphisms in LOC534614 should be performed in the future.

• Journal of the Korean Geotechnical Society
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• v.31 no.10
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• pp.29-36
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• 2015

This paper deals with inspecting and monitoring cracks developed on a subway tunnel liner during the construction of temporary supports and excavation. The cracks have developed near a enlarged part of the tunnel. Several measurements, crack gauge, internal displacement measurement, 3-D laser scanner have been conducted to monitor the progress of cracks and effects of them on the tunnel. Local measurement, additional propagation of cracks and deformation of liner, have been conducted by crack gauge and internal displacement measurement. Global inspection has been conducted by 3-D laser scanner. From the scanned data, occurrence of global deformation of tunnel and rail has been evaluated. Because of limited sequence of construction at the ground, no apparent deformation of crack propagation has been measured. As presented in this paper, deformation of tunnel liner and effects of rail need to be investigated in view of local and global aspects.

• Korean Institute of Interior Design Journal
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• v.19 no.6
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• pp.112-120
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• 2010

Human can recognize the environment by detecting spatial perception, and most of environmental perception depends on visual perception. In view that the acquisition of spatial information is accomplished through visual recognition, analysis of visual structure contained in the space is thought to be very important sector in studying the characteristic of the space. The history of studies on visual structure of space, however, wasn't too long, and furthermore most of the theories up to now focused on static and planar principles. Under this circumstance, this study is intended to suggest new theory by combining Isovist theory and VGA theory that have been actively discussed as the theory on visual perception-based spatial structure and supplementing them between each other to expand into 3-dimensional model. The suggested theory is a complex principle in dimensional and dynamic form in consideration of visual direction, which forms 3-dimentional virtual model that enables visualization of the property of spatial structure as the routine discriminating whether visual connection is made between viewing point and target point, and the target point is included in the visual field quadrangular pyramid or not. Such model was built up by an analysis application where four probe paths were applied to simulate the visual structure that occurs in virtual space, and then the characteristics were analyzed through quantification. In result, in spite of the path with equal space and equal length, significant difference in the acquired quantity of spatial information could be found depending on the probe sequence. On the contrary, it was found that probe direction may not affect the acquired quantity of information and visual property of the space.

• Journal of Intelligence and Information Systems
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• v.8 no.2
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• pp.1-13
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• 2002

In this paper, we propose an object tracking system which can be convinced of moving area shaped on objects through color sequential images, decided moving directions of foot messengers or vehicles of image sequences. In static camera, we suggests a new evaluating method extracting co-occurrence matrix with feature vectors of RGB after analyzing and blocking difference images, which is accessed to field of camera view for motion. They are energy, entropy, contrast, maximum probability, inverse difference moment, and correlation of RGB color vectors. we describe how to analyze and compute corresponding relations of objects between adjacent frames. In the clustering, we apply an algorithm of FCM(fuzzy c means) to analyze matching and clustering problems of adjacent frames of the featured vectors, energy and entropy, gotten from previous phase. In the matching phase, we also propose a method to know correspondence relation that can track motion each objects by clustering with similar area, compute object centers and cluster around them in case of same objects based on membership function of motion area of adjacent frames.

• Korean Journal of Poultry Science
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• v.39 no.4
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• pp.311-315
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• 2012

The increasing demand for Korean native chicken meat indicates that the discovery of haplotypes is very important from both economic and conservation points of view. In this study, mtDNA D-loop sequences from two crossbred Korean native chicken populations of 138 individuals were investigated. Twenty six nucleotide substitutions were identified from sequence analysis and were classified into 12 haplotypes. The haplotype H_8 represents 73.47% of Woorimatdag (chicken population) sequences, which were identified in all five Woorimatdag chicken populations investigated. The H_7 haplotype (Dhap1) for D population covers 45% sequences, which indicate maternal inheritance from black Korean native chicken. On the other hand, Chap3 and Chap4 for C population are specific haplotypes, as H_5 and H_2, respectively. Based on the network profiles, six SNPs (C199T, A239G, G242A, A291G, T330C and C391A) of the D-loop region are effective markers for discrimination between Woorimatdag and Hanhyup chicken populations. Also, the phylogenetic analyses of Woorimatdag and Hanhyup chicken populations were used to identify the genetic relationships among the haplotypes. The results presented here can be used for developing molecular markers to discriminate between two commercial Korean native chickens.

• Journal of Microbiology
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• v.41 no.3
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• pp.212-218
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• 2003

Citrus phytoene synthase (CitPsy) and ${\beta}$-carotene hydroxylase (CitChx), which are involved in caroteinoid biosynthesis, are distantly related to the corresponding bacterial enzymes from the point of view of amino acid sequence similarity. We investigated these enzyme activities using Pantoea ananatis carotenoid biosynthetic genes and Escherichia coli as a host cell. The genes were cloned into two vector systems controlled by the T7 promoter. SDS-polyacrylamide gel electrophoresis showed that CitPsy and CitChx proteins are normally expressed in E. coli in both soluble and insoluble forms. In vivo complementation using the Pantoea ananatis enzymes and HPLC analysis showed that ${\beta}$-carotene and zeaxanthin were produced in recombinant E. coli, which indicated that the citrus enzymes were functionally expressed in E. coli and assembled into a functional multi-enzyme complex with Pantoea ananatis enzymes. These observed activities well matched the results of other researchers on tomato phytoene synthase and Arabidopsis and pepper ${\beta}$-carotene hydroxylases. Thus, our results suggest that plant carotenoid biosynthetic enzymes can generally complement the bacterial enzymes and could be a means of carotenoid production by molecular breeding and fermentation in bacterial and plant systems.