• Genomics & Informatics
• /
• 제19권3호
• /
• pp.33.1-33.10
• /
• 2021

Eucalyptus is one of the major plantation species with wide variety of industrial uses. Polymorphic and informative simple sequence repeats (SSRs) have broad range of applications in genetic analysis. In this study, two individuals of Eucalyptus tereticornis (ET217 and ET86), one individual each from E. camaldulensis (EC17) and E. grandis (EG9) were subjected to whole genome resequencing. Low coverage (10×) genome sequencing was used to find polymorphic SSRs between the individuals. Average number of SSR loci identified was 95,513 and the density of SSRs per Mb was from 157.39 in EG9 to 155.08 in EC17. Among all the SSRs detected, the most abundant repeat motifs were di-nucleotide (59.6%-62.5%), followed by tri- (23.7%-27.2%), tetra- (5.2%-5.6%), penta- (5.0%-5.3%), and hexa-nucleotide (2.7%-2.9%). The predominant SSR motif units were AG/CT and AAG/TTC. Computational genome analysis predicted the SSR length variations between the individuals and identified the gene functions of SSR containing sequences. Selected subset of polymorphic markers was validated in a full-sib family of eucalypts. Additionally, genome-wide characterization of single nucleotide polymorphisms, InDels and transcriptional regulators were carried out. These variations will find their utility in genome-wide association studies as well as understanding of molecular mechanisms involved in key economic traits. The genomic resources generated in this study would provide an impetus to integrate genomics in marker-trait associations and breeding of tropical eucalypts.

• 한국과학교육학회지
• /
• 제21권5호
• /
• pp.924-943
• /
• 2001

The purpose of the study is to analyze and compare the primary science curricula of Korea, China, and Japan. Science textbooks for Korea and China and national science curriculum guides for Korea and Japan were analyzed in terms of the scope and sequence of the topics. The number of primary science topics dealt with is greatest in China, followed by Korea, then Japan. In addition to the wide range of topics, the Chinese curriculum also shows more in-depth coverage of topics. On the contrary, the Japanese curriculum has the least number of topics and shallowest depth of coverage. Korea seems to be in the middle between China and Japan. The similarities of the curricula in these East Asian countries is greatest between Korea and China. and the least between China and Japan. The similarities between Korea and Japan is somewhere in the middle. Korean primary science curriculum shows a comparatively even distribution of topics across grades. A relatively smaller number of sub-topics are introduced at each grade level, especially in the area of earth science and physics. On the contrary, in the Chinese curriculum, sub-topics tend to be concentrated at a certain grade level, thus major topics are dealt with in a grade or two. The Japanese science curriculum has fewer topics than those of the other countries, and generally one or two sub-topics appeared in a grade or two.

• 한국정보처리학회논문지
• /
• 제5권9호
• /
• pp.2378-2387
• /
• 1998

본 논문에서는 VLSI 회로의 내장 자체 테스트(Built-In Self-Test)를 위한 효율적인 공간 응답 압축기의 설계 방식을 제안한다. 제안하는 공간 압축기의 설계 방식은 테스트 대상 회로의 구조와는 독립적으로 적용할 수 있다. 기존의 공간 응답 압축기는 하드웨어 오버헤드(hardware overheads)가 크고, 고장 응답을 비고장 응답으로 변환시키는 에일리어싱(aliasing)에 의해 고장 검출률(fault coverage)을 감소시켰으나, 제안하는 방식에 의해 설계된 공간 응답 압축기는 기존의 방법에 비해 하드웨어 오버헤드가 작고, 고장 검출률을 감소시키지 않는다. 또한, 제안하는 방식은 일반적인 N-입력 논리 게이트로 확장이 가능하여 테스트 대상 회로의 출력 시퀸스에 따른 가장 효율적인 공간 응답 압축기를 설계할 수 있다. 제안한 설계 방식은 SUN SPARC Workstation 상에서 C 언어를 사용하여 구현하며, ISCAS'85 벤치마크 회로를 대상으로 선형 피드백 시프트 레지스터(Linear Feedback Shift Registers)에 의해 생성된 의사 랜덤(pseudo random)패턴을 입력원으로 사용하여 시뮬레이션을 수행하므로써 그 타당성과 효율성을 입증한다.

• Journal of Plant Biotechnology
• /
• 제37권2호
• /
• pp.153-165
• /
• 2010

Brassica rapa is considered an ideal candidate to act as a reference species for Brassica genomic studies. Among the three basic Brassica species, B. rapa (AA genome) has the smallest genome (529 Mbp), compared to B. nigra (BB genome, 632 Mbp) and B. oleracea (CC genome, 696 Mbp). There is also a large collection of available cultivars of B. rapa, as well as a broad array of B. rapa genomic resources available. Under international consensus, various genomic studies on B. rapa have been conducted, including the construction of a physical map based on 22.5X genome coverage, end sequencing of 146,000 BACs, sequencing of >150,000 expressed sequence tags, and successful phase 2 shotgun sequencing of 589 euchromatic region-tiling BACs based on comparative positioning with the Arabidopsis genome. These sequenced BACs mapped onto the B. rapa genome provide beginning points for genome sequencing of each chromosome. Applying this strategy, all of the 10 chromosomes of B. rapa have been assigned to the sequencing centers in seven countries, Korea, UK, China, India, Canada, Australia, and Japan. The two longest chromosomes, A3 and A9, have been sequenced except for several gaps, by NAAS in Korea. Meanwhile a China group, including IVF and BGI, performed whole genome sequencing with Illumina system. These Sanger and NGS sequence data will be integrated to assemble a draft sequence of B. rapa. The imminent B. rapa genome sequence offers novel insights into the organization and evolution of the Brassica genome. In parallel, the transfer of knowledge from B. rapa to other Brassica crops would be expected.

• 한국정보처리학회논문지
• /
• 제4권2호
• /
• pp.552-562
• /
• 1997

본 논문은 형식 기술 기법에 의한 차세대 지능망의 INAP(Intelligent Network Application Protocol) 프로토콜 적합성 시험 계열 생성을 위해 형식 기술 기법(formal Description Tehnique)을 사용하여 프로토콜을 명세화한후 이것으로부터 최소 길이의 최소 비용을 요구 하는 시험계열 생성을 위한 최적화 기술에 의한 방법을 제안한다. 제안된 방법을 구연하고 효율성을 증명하기 위해, INAP 프로토콜 SRF(Serivece Rexource Function)의 SRSM(SRF Call State Machine)을 형식 기술 기법 중의 하나인 SDL(System Description Language)로 명세화 하여 관련 도구로 I/O FSM(Input/Output Finite Machine)을 생성 하고, 이 참조 모델에 직접 적용하여 시험 개열을 생성하였다. 이렇게 생성 시험 개혈의 길이가 기존의 UIO(Unique Input Output)방법에 의한 31%나 개선된 짧고 효율적임을 보였고 또 오류 판단 능력면에서도 훨씬 효과가 있음을 실험적으로 증명하였다.

• Mycobiology
• /
• 제50권1호
• /
• pp.55-65
• /
• 2022

Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of ELF, no comparative investigation of an ELF community involving isolation of a pure culture and high-throughput sequencing has been conducted. Thus, we analyzed the ELF community in Parmotrema tinctorum by culture and metabarcoding. Alpha diversity of the ELF community was notably greater in metabarcoding than in culture-based analysis. Taxonomic proportions of the ELF community estimated by metabarcoding and by culture analyses showed remarkable differences: Sordariomycetes was the most dominant fungal class in culture-based analysis, while Dothideomycetes was the most abundant in metabarcoding analysis. Thirty-seven operational taxonomic units (OTUs) were commonly observed by culture-and metabarcoding-based analyses but relative abundances differed: most of common OTUs were underrepresented in metabarcoding. The ELF community differed in lichen segments and thalli in metabarcoding analysis. Dissimilarity of ELF community intra lichen thallus increased with thallus segment distance; inter-thallus ELF community dissimilarity was significantly greater than intra-thallus ELF community dissimilarity. Finally, we tested how many fungal sequence reads would be needed to ELF diversity with relationship assays between numbers of lichen segments and saturation patterns of OTU richness and sample coverage. At least 6000 sequence reads per lichen thallus were sufficient for prediction of overall ELF community diversity and 50,000 reads per thallus were enough to observe rare taxa of ELF.

• 한국방사선학회논문지
• /
• 제5권4호
• /
• pp.161-169
• /
• 2011

본 연구는 입체적 분포를 형성한 시각신경계에 대하여 MRI 시스템의 경사자기장으로써 하나의 스캔 대상단면을 3차원 형태의 여러 방향으로 향하게 하는 다중사위 스캔각도의 변화에 관하여 실험하였고 기존의 단순각도 사방향 검사방법과 비교 고찰 하였다. 입체적 분포를 이루는 뇌의 시각신경계에 대하여 MR 시스템의 경사자기장으로써 국내 정상 성인을 대상으로 시각신경의 사위영상화를 위한 기존의 사위(시상-관상단면) 스캔방법과 다중사위(시상-관상-횡단면) 스캔각도의 변화를 실험하였다. 그 결과 다중각도를 이용한 사위스캔 방법이 기존의 스캔방법에 비해 더 넓은 영역의 해부학적 정보를 나타내는 것을 영상으로 확인하였다. 또한 시각신경을 명료하게 나타내기 위해서는 영상단면두께와 펄스시퀀스의 선택도 고려되어야할 것으로 확인 되었다.

• 한국축산식품학회지
• /
• 제37권5호
• /
• pp.764-772
• /
• 2017

Fertilized hen eggs are rich in a variety of bioactive ingredients. In this study, we aimed to obtain an antioxidant protein from fertilized eggs and the radical scavenging abilities on 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (${\bullet}OH$), superoxide anion ($O^{2-}{\bullet}$) were used to evaluate the antioxidant activity of the purified protein. During 20 d of incubation, the radical scavenging ability of protein extracted from fertilized eggs exhibited significantly differences and the protein on day 16 showed higher antioxidant capacity. Based on this, the antioxidant protein of the samples on day 16 were isolated for the follow-up study. With a molecular weight 43.22 kDa, the antioxidant protein was purified by Diethylaminoethyl cellulose -52 (DEAE-52) column and Sephadex G-100. The LC-MS analysis showed that the purified protein molecular weight was 43.22 kDa, named D2-S. The sequence of amino acids was highly similar to ovalbumin and the coverage reached to 84%. The purified protein showed a radical scavenging rate of $52.34{\pm}3.27%$ on DPPH and $63.49{\pm}0.25%$ on ${\bullet}OH$, respectively. Furthermore, the C-terminal amino acid sequence was NAVLFFGRCVSP, which was consistent with the sequence of ovabumin. These results here indicated that purified protein may be a potential resource as a natural antioxidant.

• Genomics & Informatics
• /
• 제4권4호
• /
• pp.141-146
• /
• 2006

We sequenced 1,841 BAC clones by terminal sequencing, and 1,830 of these clones were characterized with regard to their human chromosomal location and gene content using Korean BAC library constructed at the Korean Science (KCGS). Sequence analyses of the 1,830 BAC clones was performed for chromosomal assignment: 1,144 clones were assigned to a single chromosome, 190 clones apparently assigned to more than one chromosome, and 496 clones to no chromosome. Evaluating gene content of the 1,144 BAC clones, we found that 706 clones represented 1,069 genes of which 415 genes existed in the BAC clones covering the full sequence of the gene, 180 genes covering a $50%{\sim}99%$, and 474 genes covering less than 50% of the gene coverage. The estimated covering size of the KBAC clones was 73,379 kilobases (kb), in total corresponding to 2.3% of haploid human genome sequence. The identified BAC clones will be a public genomic resource for mapped clones for diagnostic and functional studies by Korean scientists and investigators worldwide.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
• /
• 제16권6호
• /
• pp.712-716
• /
• 2010

본 논문에서는 레퍼런스 시퀀스에 기가 시퀀싱데이터를 매핑하여 얻어지는 커버리지 데이터를 이용한 모양 기반의 단위반복변이 영역 (CNVR) 추출 방식을 제안한다. 제안하는 CNVR 검색 알고리즘은 후보 영역 추출 단계와 후처리 단계로 이루어진다. 후보 영역 추출 단계에서는 추출하고자 하는 CNV의 모양을 입력 변수로 조절하여 다양한 높이 및 크기를 갖는 CNV 후보 영역을 추출한다. 다음, 후처리 단계에서는 레퍼런스 시퀀스와 기가 시퀀싱 데이터에 포함되어 있는 시퀀싱 에러 문제를 보완하기 위하여, 레퍼런스 시퀀스의 에러 영역 보정, GC-content 영역 보정 등의 정제 과정을 거친 후, 최종 CNVR을 추출한다. 제안된 방식의 유용성을 보이기 위하여 "1000 게놈 프로젝트"에 의하여 공개된 실 데이터를 이용한 다양한 실험을 수행하였으며, DGV를 이용하여 추출된 CNVR의 정확도를 검증하였다. 실험 결과에 의하면 제안된 방식은 HLA 영역에 존재하는 반복되거나 결실되는 다양한 모양의 CNV를 효율적으로 검출하였다.