• BMB Reports
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• 제45권4호
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• pp.233-238
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• 2012

We present a top down separation platform for yeast ribosomal proteins using affinity chromatography and capillary electrophoresis which is designed to allow deposition of proteins onto a substrate. FLAG tagged ribosomes were affinity purified, and rRNA acid precipitation was performed on the ribosomes followed by capillary electrophoresis to separate the ribosomal proteins. Over 26 peaks were detected with excellent reproducibility (<0.5% RSD migration time). This is the first reported separation of eukaryotic ribosomal proteins using capillary electrophoresis. The two stages in this workflow, affinity chromatography and capillary electrophoresis, share the advantages that they are fast, flexible and have small sample requirements in comparison to more commonly used techniques. This method is a remarkably quick route from cell to separation that has the potential to be coupled to high throughput readout platforms for studies of the ribosomal proteome.

• 한국실내디자인학회논문집
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• 제41호
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• pp.96-103
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• 2003

The historical separation between organization studies and architecture area created a design environment in which wort processes are evaluated separately from setting where they occur. The impact of separation has been parallel yet disconnected development in th two disciplines in which the advance in one arena have not precipitated advances in the other. This is the unfortunate loss as each discipline has the potential to contribute directly to the other. With the emergence of new economy the opportunity to realign the tow disciplines is once again presented as new ways of working have enhanced this opportunity. It is important to revisit the historical development of the tow disciplines and to discuss how the misalignment of their respective concerns contributed to the establishment of our current situation. What happened to create an environment in which organizations assume that one of their largest capital expenditures the cost of providing work spaces for employees is unrelated to their work process\ulcorner what recent developments offer opportunities to rectify this dilemma\ulcorner In this study it is presented a historical review of organization studies and architecture. Through examination of these areas across time it becomes quite apparent that it is now appropriate to pose new questions about organization and their architecture. The comparison of two parallel developments explains how the current design environment is separated from organization studies and offers compelling evidence for why it is important to revisit this separation in light of contemporary theories on collaborative work, organizational learning and communities of practice.

• 한국지능시스템학회논문지
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• 제24권2호
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• pp.209-217
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• 2014

Of several approaches for future Internet, separating two properties of IP address into locator and identifier, is being considered as a highly likely solution. IETF's LISP (Locator ID Separation Protocol) is proposed for this architecture. In particular, the LISP model easily allows for device mobility through simple update of information at MS (Mapping Server) without a separate protocol. In recent years, some of the models supporting device mobility using such LISP attributes have emerged; however, most of them have the limitation for seamless mobility support due to the frequent MS information updates and the time required for the updates. In this paper, PMIPv6 (Proxy Mobile IPv6) model is applied for mobility support in LISP model. PMIPv6 is a method that can support mobility based on network without the help of device; thus, this we define anew the behavior of functional modules (LMA, MAG and MS) to fit this model to the LISP environment and present specifically procedures of device registration, data transfer, route optimization and handover. In addition, our approach improves the communication performance using three tunnels identified with locators between mobile node and corresponding node and using a route optimized tunnel between MN's MAG and CN's MAG. Finally, it allows for seamless mobility by designing a sophisticated handover procedure.

• 멤브레인
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• 제24권6호
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• pp.448-452
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• 2014

올레핀/파라핀 분리 기술로 silver nanoparticles(AgNPs)를 운반체로 사용하여 보다 효과적으로 올레핀을 분리하는 고성능 올레핀 촉진수송 분리막을 제조하고자 하였다. 기존에 성능이 밝혀진 PVP/AgNPs/TCNQ 나노복합체 막에 추가적으로 할로겐 물질을 첨가하여 AgNPs의 표면을 더 양극화시킴으로써 성능을 향상시키고자 하였다. 제조한 용액을 TEM과 EDS로 분석해서 AgNPs의 형성과 iodine의 존재를 확인하였다. Propylene/propane 혼합기체의 분리 성능 실험을 통해 기존 PVP/AgNPs/TCNQ 나노복합체 분리막과 기체 분리 성능을 비교하였고, long-term stability 실험을 통해 분리막의 안정성을 조사하였다.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.426-432
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• 2004

Large-Eddy Simulation (LES) is applied for the simulation of compressible flat plate boundary with Reynolds number up to 5 X 10$^{5}$ . Numerical examples include shock/boundary layer interaction and boundary layer transition, aiming future application to the analysis of transonic fan/compressor cascades. The present LES code uses hybrid com-pact/WENO scheme for the spatial discretization and compact diagonalized implicit scheme for the time integration. The present code successfully predicted the bypass transition of subsonic boundary layer. As for supersonic turbulent boundary layer, mean and fluctuation velocity of the attached boundary, as well as the evolution of the friction coefficient and the displacement thickness both upstream and downstream of the separation region are all in good agreement with experiment. The separation point also agreed with the experiment. In the simulation of the shock/laminar boundary layer interaction, the dependence of the transition upon the shock strength is reproduced qualitatively, but the extent of the separation region is overpredicted. These numerical examples show that LES can predict the behavior of boundary layer including transition and shock interaction, which are hardly managed by the conventional Reynolds-averaged Navier-Stokes approach, although there needs to be more effort before achieving quantitative agreement.

• 한국막학회:학술대회논문집
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• 한국막학회 2003년도 The 4th Korea-Italy Workshop
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• pp.107-114
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• 2003

This study investigated the effect of 2-methoxy ethanol (2-Me) non-solvent as additive included in casting solution. Macroporous polymer membranes were prepared by using polyethersufone (PES)/N-methyl-2-pyrrolidone (NMP)/2-Me casting solution and water coagulant. The phase separation co-process of the vapor-induced phase separation (VIPS) and liquid-induced phase separation (LIPS) were used by means of membrane preparation method. The pore size and pore size distribution were controlled with additive (non-solvent), and measured with Automated Perm Porometer. By increasing additive (non-solvent) in the casting solution, the membranes produced changed from finger structure to sponge structure. That is due to the different diffusion rates. At slow diffusion process, sponge-like structure was formed and at fast diffusion process, finger-like structure was formed. Also relative humidity, evaporation time, temperature of casting solution and coagulation bath etc. had effects on the pore size distribution and the porosity of the membrane.

• 한국통신학회논문지
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• 제16권12호
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• pp.1324-1335
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• 1991

본 논문은 음절을 음소로 분리하고 각각의 특징을 추출하여 음소를 판별할 수 있는 패턴을 설정하는 실험에 관한 연구이다. 음소분리는 피치검출, 각 성문피크펄스의 폭, 포락실, 진폭의 바이어스, 발성시간 등의 파라메타를 적용한다. 최초의 피치는 성문펄스의 폭, 에너지, 정규화와 성문피크의 가변바이어스 등의 변화에 따라 모음 포라선의 봉우리 부분에서 검출, 이를 기준으로 전체의 피치구간을 추적한다. 모음은 포만트 패턴의 유동을 감소시키는 방법과 제2포만트만으로 모음의 판별이 가능함을 제시하며, 피치 고저에 무관한 압축파형을 추정한다. 자음은 포락실, 스펙트럼, 압추파형, 분석방법 등을 발음방법과 음소 상호 영향에서 패턴을 추출한다. 실험결과 모음음소 90%, 초성자음80%, 종성자음 60% 판별된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
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• pp.37-38
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• 2007

It needs several large reservoirs and long time to remove suspended solid like steel fines and iron oxide in hot rolling coolant. If removing rate of suspended solid in rolling coolant is improved by using high gradient magnetic separation (HGMS) system, the productivity of working process can be increased and the area of reservoir can be reduced. Pre-treatment process that react magnetic floc with inorganic and organic flocculant in coolant was studied. Horizontal type superconducting HGMS system was manufactured successfully, which was constituted with automatic filter transportation apparatus, 6 T He-free superconducting magnet system and water circulation system. The solid suspension removal ratio in hot rolling coolant was increased by flocculation with inorganic and organic flocculant.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.276-276
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• 2013

Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (~104) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2012년도 제38회 춘계학술대회논문집
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• pp.24-27
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• 2012

본 연구에서는 격벽형 펄스분리장치의 성능을 검증하기 위하여 실물형 Flight-type의 이중펄스 로켓모타 추진기관을 설계, 제작하여 지상연소시험을 수행하였다. 지상연소시험 시 계측한 추진기관의 압력, 추력, 진동 결과를 바탕으로 펄스분리장치의 파열특성인 파열시간과 파열압력 분석을 실시하였고 연소시험 후 3차원 측정기를 이용한 삭마량 측정을 실시하였다. 그 결과, 펄스분리장치가 파열조건 및 열적 안전성을 만족하는 것을 확인할 수 있었다.