• Journal of Microbiology and Biotechnology
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• 제18권12호
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• pp.1953-1957
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• 2008

Recombinant Lactobacillus strains have been constructed using gene splicing by overlap extension (SOE). Primers were designed of which one end of an amplified product contained complementary sequences for an end of other amplified fragment. For efficient matching, we used an asymmetric PCR step that was effective at generating an excess of strands that would anneal in the final PCR. CP12, a recombinant fragment consisting of the integrase gene and attachment site of the bacteriophage A2, was constructed and inserted into the genome of Lactobacillus casei ATCC 393, yielding Lb. casei ATCC 393::XCP12. Another recombinant Lb. casei strain was constructed, where the egfp gene was a part of the construction. The EGFP produced from Lb. casei ATCC 393::XCEGFP14 was detected by Western blot hybridization. This simple and widely applicable approach has significant advantages over standard recombinant DNA techniques for Lactobacillus species.

• 한국대기환경학회지
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• 제17권2호
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• pp.147-155
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• 2001

ISCLT3 model was applied in Seoul metropolitan area to investigate the source-receptor relationships among 17 cities in the Kyonggi Province. For the purpose of the model simulation, emission rates of NO$_2$, SOx, and PM(sub)10 were prepared for grid scale with 1$\times$1 km, and receptors were located on every 2$\times$2 km grid. Meterological data for the last 10 years(88~97) were used as input data. According to our study, NO$_2$ concentration of the cities ranged from 10 to 45 ppb with the highest value appearing from Puchon city. The concentrations of SOx and PM(sub)10 concentrations fell in the range of 5~20 ppb and 20~70$\mu\textrm{g}$/㎥, respectively. It was also found out that air quality in one city can be affected greatly by the air pollutants originating from other cities.

• 아동학회지
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• 제33권5호
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• pp.19-35
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• 2012

The purposes of this study were to investigate how children's categorization differs in object categorical hierarchy and to examine whether these aspects were different according to the children's age of 18 months, 24 months, 30 months. The participants consisted of 120 young children aged 18 months, 24 months, and 30 months from 31 child-care centers located in middle-income regions of Seoul and Kyonggi Province. The major findings were as follows : First, all the children from all three age groups could consistently differentiate the superordinates; however, they could not consistently differentiate basic categories. Second, 24 month appears to be a critical change period in category development. Third, as the children become older, they are able to acquire more knowledge regarding categories. These results suggested that the advent of ordering, in terms of basic categories as well as superordinates which occurred around the age of 24 month, was confirmed in category development.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2023년도 추계학술발표대회
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• pp.930-931
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• 2023

본 프로젝트는 색상 산업 분야에서 효율적인 업무 생산을 할 수 있도록 하는 산업용 로봇을 제시한다. 모바일 어플리케이션 개발을 통해 사용자 맞춤 UI를 제공하여 색상 조합 및 제안을 수행하도록 하며, 객체 인식 기술을 적용하여 자동화 시스템을 거쳐 색상 출력을 수행할 수 있는 색 조합 시뮬레이터 자율 주행 로봇이다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 추계학술대회 논문집
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• pp.1900-1906
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• 2008

신도시 개발 등으로 대도시의 생활권이 확대됨에 따라 수도권 교통의 광역화가 지속적으로 진행되고 있어 대도시권내의 광역교통문제해소 및 혼잡비용 최소화를 위한 체계적이고 지속 가능한 장기 마스터플랜 구상이 필요하게 되었다. 이에 따라, 최근 경기도에서는 동탄 신도시와 서울 강남을 20분대에 연결할 수 있는 대심도 급행전철 사업을 구상하였다. 국토부에서는 경부고속철도 2단계와 호남고속철도 완공에 따른 선로용량 부족해소와 수도권 및 경기 남부 지역 주민들에게 고속철도 서비스제공을 위한 강남권 고속철도 노선(수서${\sim}$동탄${\sim}$평택) 건설이 필요한 것으로 "수도권 철도망 개선방안 연구용역(2007.12)" 결과를 발표하였다. 경기도에서 추진 중인 광역급행전철과 강남권 고속철도 노선이 중복됨에 따라 국토의 이용 효율성 향상과 국가재정의 효율적 투자를 위해 고속철도의 선로 여유용량을 활용한 광역급행전철 혼용에 대한 검토가 필요할 것으로 판단된다. 따라서, 본 연구에서는 고속철도와 광역급행전철을 혼용 운행할 경우 노반분야 및 E & M 기술조건, 중간역 계획, 열차운영계획 등의 분석을 통해 최적화된 혼용운영 방안과 사업비 절감효과 등을 제시함으로써 동일선로에서 이종의 열차가 운행됨에 따른 철도사업의 투자효율성 제고 방안을 제시하고자 한다.

• 융합경영연구
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• 제12권2호
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• pp.11-18
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• 2024

Purpose: The purpose of this study is to suggest the activation plan of the post-construction sales through the results of a survey on the perception of Seoul citizens and experts. Research design, data and methodology: The purpose of this study is to suggest the activation plan of the post-construction sales through the results of a survey on the perception of Seoul citizens and experts. Results: According to a survey of Seoul citizens' perceptions, 76.7% of Seoul citizens were well aware of post-construction sales and recognized that post-construction sales would reduce pre-sale speculation and confusion in the real estate market. Second, 73.6% of Seoul citizens were willing to buy houses through post-construction sales, and third, 79.6% of Seoul citizens recognized that a post-sale system was necessary. Experts' opinions generally responded to the expansion of the introduction of post-construction sales, saying, 'It is necessary for both the public and the private sectors'. Second, while experts say that there are also positive effects, negative effects such as polarization centered on large corporations, an increase in sales prices, and a decrease in housing supply are also concerned. Third, experts responded that 'diversification of financing methods' is the most important task in revitalizing the post-sale system. Conclusions: The policy implications are that it is necessary to mandate the post-construction sales in the long term, and that the quality assurance system needs to be supplemented even if the sale is promoted post-construction sales. In addition, private participation is essential to revitalize the post-construction sales, and government support such as initial financing, low-interest rates, and various financing measures should be sought to expand private participation.

• 한국재료학회지
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• 제5권3호
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• pp.280-287
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• 1995

첨가제로 $Al_{2}$$O_{3}$가 포함된 ZnO 소결체가 타깃을 이용하여 RF 마그네트론 스퍼터링법으로 Al이 첨가된 ZnO박막을 증착하고, 타깃에 첨가된 $Al_{2}$$O_{3}$의 농도와 증착시 스퍼터링장치내의 기판위치에 따른 박막의 물성 변화를 고찰하였다. 타깃의 $Al_{2}$$O_{3}$ 첨가농도가 2wt%인 경우에 비저항치 8 $\times$ $10_{-3}$ $\Omega$-cm인 박막이 증차되었다. 또한 $Al_{2}$$O_{3}$가 2wt%이상 첨가된 경우는 모든 Al이 박막내부에서 Zn를 치환하여 전자주게로의 역할을 하지 못하고, 오히려 치환되지 못한 Al원자의 중성 불순물 산란효과에 의해 박막의 비저항이 증가하였다. 타깃의 마모영역 위에서 증착된 Al을 첨가한 ZnO 박막은 그 영역 KR에서 증착된 박막보다 높은 비저항값을 나타냈으며, 이는 큰 에너지를 가지는 산소입자의 충돌에 기인한 것으로 여겨진다.

• 대한수의학회지
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• 제9권2호
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• pp.7-18
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• 1969

Researches to determine the infectious status on the pancreatic fluke, Eurytrema pancreaticum(Janson 1889) Looss, 1907, were made into several slaughter hauses at four different districts through seven years from 1963 to 1969. The infection rate showed 2962 positive of 5974 heads (49.6%) of cattle at the Seoul first slaughter hause in Seoul, 881 positive of 1021 heads (86.3%) in Cheju, and six(6) positive of 56 heads (11.1%) at Suwon, 58 positive of 306 heads(19.0%)at Joenju. The infection rate 53.1%, (2907 positive heads of total 7357 heads exammed) is the representative infection rate at the cattle eurytremiasis in Korea. It showed little variation through a year and was increased as compared with those of the past period, from 1915 to 1945. Fecal examinations in order to find out the local distribution of the pancreatic fluke were conducted on cattle, sheep, and goats raised in four districts, showing two(2) of 269 heads (0.7%) in Kyonggi-do, 41 of 212 heads (19.3%) in Cheju-do, and negative in both Chungnam-do and Joenbuk-do. When an artificial infection wss performed on rabbits and goats with metacercaria of the pancreatic fluke, Parenchyma of their pancreas were degenerated and the ducts were dilated. When cattle and sheep were infected naturally, the pancreatic ducts were also dilated, the wall was thickened, and adenomatous and epithelial hyperplasia was appeared. The conclusion, putting the foregoing pathological changes, has been arrived at chronic hyperplastic pancreatitis in the eurytremiasis of cattle, sheep, goats and rabbits.

• 대한건축학회논문집:계획계
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• 제34권3호
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• pp.117-128
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• 2018

The purpose of this study is to identify the physical safety factors and indicators needed to create an age-friendly city where elderly people can live safely and continuously in the era of aging and to evaluate the differences between the five urban communities. For the study, AHP analysis was conducted to calculate the importance of factors and indicators, based on a checklist was made this. A questionnaire survey was conducted on the degree of physical safety perceived by the elderly people aged 65 and older living in Seoul, the difference between the five urban communities in Seoul was confirmed. The research finding are summarized as follows: The score of evaluating grade was the highest in the northwest, northeast was the lowest followed by the northwest, downtown, southeast, southwest, northeast. Among the physical safety factors, the safety of outdoor spaces and buildings was the highest in the downtown and the lowest in the southwest. On pedestrian safety, the downtown was the highest and the lowest was northeast. Regards to the transportation safety, the northwest was the highest and the northeast was the lowest. Housing security was the best in the northwest and the lowest was the northeast.

• Food Science and Biotechnology
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• 제18권2호
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• pp.396-401
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• 2009

The nucleotide sequence contains 2 open reading frames encoding a 45-amino-acid protein homologous to a transcriptional repressor protein CopG, and a 203-amino-acid protein homologous to a replication protein RepB. Putative countertranscribed RNA, a double-strand origin, and a single-strand origin were also identified. A shuttle vector, pUCL2.1, for various lactic acid bacteria (LAB) was constructed on the basis of the pCL2.1 replicon, into which an erythromycin-resistance gene as a marker and Escherichia coli ColE1 replication origin were inserted. pUCL2.1 was introduced into E. coli, Lc. lactis, Lactobacillus (Lb.) plantarum, Lb. paraplantarum, and Leuconostoc mesenteroides. The recombinant LAB maintained traits of transformed plasmid in the absence of selection pressure over 40 generations. Therefore, pUCL2.1 could be used as an E. coli/LAB shuttle vector, which is an essential to engineer recombinant LAB strains that are useful for food fermentations.