• The Korean Journal of Mycology
• /
• v.51 no.4
• /
• pp.361-371
• /
• 2023

The study was undertaken to isolate and characterize wild yeast strains from soil samples collected in Seoul, Korea. Among the 19 yeast strains obtained, 17 were previously recorded species. The remaining two strains, Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 were new species candidates. The genomic and microbiological characteristics of GW1-4 and YB4-103 were investigated. Phylogenetic analyses based on the 26S rRNA gene sequences and internal transcribed sequences, GW1-4 and YB4-103, represent a distinct lineage within the family Phaffomycetaceae and Archaeorhizomycetaceae, respectively. The GW1-4 and YB4-103 strains had the highest sequence homology with Wickerhamomyces xylosivorus NBRC 111553^T (88.97%) and Archaeorhizomyces finlayi CBS 128710^T (87.55%), respectively.

• Journal of Nutrition and Health
• /
• v.1 no.1
• /
• pp.52-54
• /
• 1968

• Development and Reproduction
• /
• v.17 no.4
• /
• pp.441-449
• /
• 2013

Recent study showed that T cells in the immune organs and peripheral blood are influenced by estradiol, leading to a dysfunction of the immune system. However, little is known about the thymic-gonadal relationship during the estrous cycle in mouse. Therefore, the purpose of this study was to elucidate the mechanism by which a change in estradiol levels during the estrous cycle regulates the development of T cells in the mouse thymus. Six-week-old ICR mice were used and divided into four groups, including diestrous, proestrous, estrous, and metestrous. We first confirmed that ER-${\alpha}$ and - ${\beta}$ estrogen receptors were expressed in thymic epithelial cells, showing that their expression was not different during the estrous cycle. There was also no significant difference in thymic weight and total number of thymocytes during the estrous cycle. To determine the degree of thymocyte differentiation during the estrous cycle, we analyzed thymocytes by flow cytometry. As a result, the percentage of CD4+CD8+ double-positive (DP) T cells was significantly decreased in the proestrous phase compared to the diestrous phase. However, CD4+CD8- or CD4-CD8+ (SP) T cells were significantly increased in the proestrous phase compared to the diestrous phase. In addition, the percentage of CD44+CD25- (DN1) T cells was significantly decreased in the estrous phase compared to other phases, whereas the percentages of CD44+CD25+ (DN2), CD44-CD25+ (DN3), and CD44-CD25- (DN4) were not changed during the estrous cycle. These results indicate that the development of thymocytes may arrest in the DP to SP transition stage in the proestrous phase displaying the highest serum level of estradiol. This study suggests that a change in estradiol levels during the estrous cycle may be involved in the regulation of thymocyte differentiation in the mouse thymus.

• Journal of Community Nutrition
• /
• v.3 no.2
• /
• pp.110-119
• /
• 2001

This study was designed to assess the needs for nutrition education and educational materials for older adults. Two cross-sectional surreys were conducted. The first survey, conducted by personal interviews, was part of the large-scale elderly nutrition study. Subjects were adults aged 50 and over, recruited from 6 large cities and 8 middle-sized cities(n = 1,850). The second survey, done by mail survey using open-ended questions, was conducted with dietitians working at public health centers or hospitals(n = 53). Adults aged 50 and over were interested in topics such as healthly eating(32.1%), hypertension/stroke and diet(22.1%), osteoporosis and diet(11.4%), and diabetes and diet(9.2%). Television and radio(58.2%), health professionals(12.2%) and friends(7.9%) were common sources of nutrition information. Preferred topics of nutrition education and sources of nutrition information were different by general characteristics of subjects, suggesting that nutrition education or educational materials be planned considering the characteristics of subjects. About 70% of subjects indicated that they sometimes use or do not use nutrition information in daily lives, suggesting the need to provide more practical information. Among 53 facilities responding to the second survey, 73.6% provided nutrition education for older adults. Common topics for nutrition education included diabetes(39.3%), hypertension and stroke(19.1%) and general nutritional management(11.2％). These were consistent to the topics preferred by older adults. As materials In elderly education, dietitians wanted primarily to use leaflets and slides. Boards, booklets and posters were other commonly cited materials. For contents of elderly educational materials, dietitians mentioned the nutritional management for age-related diseases(33.8%), general nutritional management for older adults(25.4%) and practically applicable information(19.7%). They also suggested that nutrition education materials for the elderly should use larger print and attractive pictures, and be easily understood, as well as presenting simple, specific and practical information. These results provide baseline information for developing nutrition education and educational materials for older adults.

• Development and Reproduction
• /
• v.14 no.4
• /
• pp.233-241
• /
• 2010

Human adipose stem cells are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue and these cells have characteristics very similar to bone marrow mesenchymal stromal cells (BMMSCs). However, liposuction procedure, donor age, body mass index, and harvesting sites might generate differences in the initial cell population and the preparations are a heterogeneous mixture of precursors with different subsets. Therefore, in this study, we investigated the characteristics of human thigh adipose stem cells and the differentiation potential into mesodermal and endodermal lineage. Thigh adipose stem cells maintained fibroblast-like morphology similar to BM-MSCs and they underwent average 56.5 doublings and produced $5{\times}10^{22}$ cells. These cells expressed SCF, Oct4, nanog, vimentin, CK18, FGF5, NCAM, Pax6, BMP4, HNF4a, nestin, GATA4, HLA-ABC, and HLA-DR genes at p3 and they also expressed Oct4, Thy-1, FSP, vWF, vimentin, desmin, CK18, CD54, CD4, CD106, CD31, a-SMA, HLA-ABC proteins. Moreover, they could differentiate into mesodermal lineage cells such as adipocyte, osteoblast and chondrocyte. In addition, they also differentiated into insulin secreting cells in our culture condition. In conclusion, human thigh adipose stem cells retain proliferative potential and expression patterns similar to BM-MSCs and they also differentiate into various cell types. Thus, human thigh adipose stem cells might be useful alternative cell source for clinical application.

• Journal of Food Hygiene and Safety
• /
• v.35 no.2
• /
• pp.177-182
• /
• 2020

Copper possesses antimicrobial properties against certain bacteria and fungi. In this study, changes in microbial population, color and the antioxidant properties of dried red pepper powder (RPP) by contact with the surface of a copper plate were investigated. Lightness of RPP increased after treatment on the copper plate for 2 h at 55℃. The numbers of total aerobic mesophilic bacteria in RPP increased by 0.61 and 0.50 log on the surface of stainless steel and copper plates during 24 h at 37℃, respectively. The population of yeast and mold was reduced by 90% in the RPP treated on both copper and stainless steel plates under the same condition. Yeast and mold were significantly reduced on the copper plate during 2 h at 55℃. Scavenging activities of RPP treated on the copper plate for 24 h at 37℃ against DPPH radical were decreased; those for 2 h at 55℃ against ABTS radical were rather enhanced. The results suggest that copper might be applied in the processing and storage equipment in relation to RPP products for antimicrobial purposes.

• Development and Reproduction
• /
• v.19 no.4
• /
• pp.243-252
• /
• 2015

The pregnancy and abortion process involves a complex mechanism with various immune cells present in the implantation sites and several hormones associated with pregnancy, such as leptin, ghrelin and nesfatin-1. However, the mechanism underlying spontaneous abortion by maternal T helper 17 (Th17) present in the implantation sites and nesfatin-1, which is of anorexigenic hormones, is not fully understood so far. Therefore, the purpose of this study was to examine the possible roles of Th17 cells present in the implantation sites and nesfatin-1 expressed in the uterus on spontaneous abortion using the $CBA/j{\times}DBA/2$ mouse model. Th17 transcription factor, ROR-${\gamma}t$ mRNA expression was significantly increased in the abortion sites compared with the implantation sites of abortion model mice on day 14.5 and 19.5 of pregnancy. In addition, the expression levels of IL-17A mRNA were significantly higher in abortion sites than in implantation sites on day 14.5 and 19.5. Moreover, the nesfatin-1/NUCB2 protein and mRNA levels were increased in abortion sites compared with levels in implantation sites of both normal pregnant and abortion model mice on day 14.5 of pregnancy. Interestingly, nesfatin-1/NUCB2 serum levels were not changed throughout the whole pregnancy in abortion model mice, but its serum level was dramatically increased on day 14.5, and then rapidly decreased on day 19.5 in normal pregnant mice. In this study, we showed for the first time the expression of nesfatin-1/NUCB2 mRNA and protein in implantation sites during pregnancy. The present results suggest that Th17 cells in the uterus may play an important role in the period of implantation and for maintenance of pregnancy. Furthermore, the present results suggest that Th17 cells in implantation sites may be a key regulator for maintenance of pregnancy and provides evidence that activation of these cells may be regulated by nesfatin-1/NUCB2. Further study is needed to elucidate the role of nesfatin-1 expressed in the uterus during pregnancy.

• Korean Journal of Food Science and Technology
• /
• v.47 no.3
• /
• pp.299-305
• /
• 2015

The Folin-Denis assay using the Folin-Ciocalteu (F-C) reagent has been commonly used for analyzing the total phenolic compound content in various food products. In the present study, the effects of proteins on the reactivity of the F-C reagent with different phenolic compounds were investigated. Bovine serum albumin (BSA) or skim milk proteins showed a concentration-dependent increase in color response in the Folin-Denis assay; these proteins decreased the color response of most phenolic compounds tested. The reactivity of phenolic compounds was significantly less pronounced in the presence of BSA and this interference was greater at higher concentrations of phenolic compounds. The reactivity of phenolic compounds with the F-C reagent was reduced significantly by their oxidation; the reaction of the oxidized products with the F-C reagent was more severely affected by BSA. The interfering effects in the Folin-Denis assay might be attributable to binding interactions of phenolic compounds with proteins.

• Women's Health Nursing
• /
• v.17 no.4
• /
• pp.369-377
• /
• 2011

Purpose: Childbirth self-efficacy plays an important role in women's ability to cope with labor and delivery. Coaching has been gaining popularity as a way to promote cognitive, emotional and behavioral change. This study aimed to test the effects of a Coaching-based childbirth program on anxiety and childbirth self-efficacy among primigravida women. Methods: The study design was a quasi-experimental pre-post design. A coaching-based childbirth program was developed synthesizing concepts and techniques from the literature on coaching and was verified by an expert panel. It consisted of four weekly 2-hour small group sessions. Pregnant women were recruited from H hospital in Seoul. Childbirth self-efficacy and state anxiety were measured before and after the program. Results: Although there was a no significant reduction in anxiety, there were statistically significant increase for childbirth self-efficacy in the experimental group when compared to the control group. Conclusion: The program appears to increase childbirth self-efficacy for pregnant women. Future studies may benefit from using mixed coaching modalities and consider measuring health behaviors and obstetric outcomes to gain insights on its long-term impact.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2002.07a
• /
• pp.237-237
• /
• 2002