• Journal of Species Research
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• v.5 no.1
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• pp.22-26
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• 2016

Five bacterial strains designated DY37, BS333, JJ521, BM1, and DG13-2 were assigned to the genus Deinococcus were isolated from forest soil samples collected from Deogyusan, Busan, Changwon, and Seoul of South Korea. The isolates were Gram-staining negative or positive, and pale pink- or red-pigmented, short-rod shaped. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains DY37, BS333, JJ521, BM1, and DG13-2 were most closely related to Deinococcus aquatilis CCM $7524^T$ (with 99.0% similarity), D. ficus CC-FR2-$10^T$ (100.0%), D. grandis KS $0485^T$ (99.2%), D. roseus TDMA-$uv51^T$ (98.9%), and D. yunweiensis $YIM007^T$(100.0%), respectively. These 5 species have never been proposed in Korea; therefore 5 species of 1 genera in the family Deinococcaceae in the order Deinococcales within the class Deinococci are reported for proteobacterial species found in Korea.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.140.1-140.1
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• 2003

Permanent cell culture lines derived from human cancer tissue are important experimental models in the study of human cancer cell proliferation. The in vitro effects of C. militaris and its extracted fractions on the human breast cancer (SK-BR3), liver cancer (SK-Hep1, HepG2), kidney cancer (p15), lymphoma (Jurkat) were studied. F1 (CCCA, crude cordycepin containing adenosine), F2 (ethanol precipitation), F3 (ethanol soluble supernatant) and F4 (fraction of through SK-1B) significantly stimulated in vitro cytotoxic in human cancer cell lines. (omitted)

• Proceedings of the Korea Information Processing Society Conference
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• 2020.05a
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• pp.191-194
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• 2020

본 연구에서는 안전하면서도 쉽게 사용할 수 있는 블록체인 기반의 중고 책 거래 플랫폼을 개발하였다. 이 플랫폼은 블록체인의 특성을 이용해 거래 제품 및 거래내역을 공유원장에 기록하여 거래내역을 투명하게 공개하며 누구나 열람 가능하다. 이는 데이터의 무결성과 투명성을 보장하며 신뢰성 있는 거래 환경을 제공한다. 이를 사용할 경우, 기존 중고거래 시스템에서 발생 가능한 데이터의 위조, 변조의 문제점과 불확실한 신뢰 문제가 해결되어 중고거래 사기사건을 예방할 수 있다.

• Proceedings of the Korea Information Processing Society Conference
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• 2024.05a
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• pp.432-433
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• 2024

오늘날 중요한 키워드로 꼽히는 블록체인은 탈중앙화를 핵심으로 하는 기술이며, DApp은 블록체인 플랫폼 기반의 분산형 애플리케이션이다. 본 논문에서는 블록체인 기반의 코인 시스템이 구축된 대학생 커뮤니티 애플리케이션을 개발하고자 한다. 애플리케이션의 사용자들은 각 게시판에서 지식과 정보를 공유하고 코인을 획득할 수 있다. 코인 시스템은 블록체인 네트워크와 애플리케이션이 연결됨으로써 애플리케이션을 통해 스마트 컨트랙트를 발행하는 방식이다. 또한, 블록체인 기술을 통해 유효한 가치를 가진 글은 영구적으로 보관할 수 있으며 획득한 코인으로는 현실의 재화로 교환할 수 있다. 본 애플리케이션을 통해 대학생들이 더 넓은 소통 창구를 가질 수 있을 것으로 기대된다.

• Biomolecules & Therapeutics
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• v.6 no.4
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• pp.378-382
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• 1998

In the previous screening on antidiabetic effect of Lycii fructus by glucose transport method using $N_2$-STZ diabeted rat model, each extracts showed the potent antidiabetic activity. We obtained three compounds isolated from the water fraction, EtOAc fraction and n-BuOH fraction of Lycii fructus in the present work and their structures were identified as 1-carboxy-N,N,N-trimethylmethanaminium hydroxide inner salt, 2,4(1H,3H)-pyrimidinedione and 3,3',4',5,7-pentahydroxyflavone-3-rutinoside . Among the constituents separated from Lycii fructus, 2,4(IH,3H)-pyrimidinedione, 3,3',4',5,7-pentahydroxyflavone-3-rutinoside and ascorbic acid were shown a remarkable antidiabetic effect.

• Korean Journal of Food Science and Technology
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• v.43 no.6
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• pp.754-759
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• 2011

Zinc protoporphyrin (ZnPP) is produced endogenously during heme metabolism and treated in cells as a heme oxygenase inhibitor. In the present study, the effects of ZnPP on the color response of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a commonly-used method for analyzing cell viability, were investigated. ZnPP induced rapid decolorizaion of MTT formazan under light; the degradation rates were 10- and 20- folds faster in the presence of 5 and $10{\mu}M$ ZnPP, respectively. Methylene blue (MB), another type of photosensitizer, also accelerated degradation of formazan under light. Butylated hyroxytoluene did not inhibit ZnPP- or MB-induced formazan degradation. The color degradation of formazan dye was signficantly delayed in the presence of N-acetylcysteine or ${\beta}$-carotene. The present results suggest that certain photosensitizing compounds may affect the color and stability of MTT formazan, which should be carefully considered when conducting the MTT assay.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.11a
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• pp.119-125
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• 1994

• Journal of Environmental Health Sciences
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• v.23 no.2
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• pp.12-23
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• 1997

The biosynthesis of galactolipid, galactose and the fatty acid composition in E. cdi and B. subtills treated with potassium dichromate(PD, 500 ppm, 500 ppm), potassium chromate(PC, 500 ppm, 500 ppm), cobalt chloride(CC, 100 ppm, 10 ppm) and methylmercuric chloride(MC, 100 ppm, 10 ppm) during the culture were analyzed to compare with the control. The growth rate of cells, the contents of monogalactosyldiglyceride(MGDG), digalactosyldiglyceride(DGDG) and total lipid in the metal compound treatments were lower as compared with the control. And too, the contents of galactose utilized for the biosynthesis of galactolipids in these strains in the various metal compounds treatments were inhibited. The fatty acids used for the MGDG and DGDG formation in E. coli and B. subtills treated with each metal compounds during the culture were showed to the variant compositional change.

• Proceedings of the Korean Nutrition Society Conference
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• 1997.11b
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• pp.30-30
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• 1997

• Animal cells and systems
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• v.8 no.2
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• pp.97-103
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• 2004

The cytotoxic effect of iron was examined in peritoneal macrophage to determine contributing factors by iron injection to rat. Viability was reduced by 24% by the iron-overload and by 30% by short-term iron addition. Total iron was increased by 45% in the iron-overloaded with remarkable elevation (9 to 14 fold) in the presence of $FeSO_4$. Free calcium was also increased by 19% in control and 44% in iron-overloaded group due to additional $FeSO_4$ NO and MDA were increased by 40% and 136%, respectively, with significant reduction (37%) of NAD(P)H. RCR and cytochrome c oxidase activity were lowered approximately by 10% with reduction of mitochondrial membrane potential. Addition of iron was frequently associated with altered distribution of mitochondria of high membrane potential in the iron-overloaded macrophage. These results suggest altered mitochondria with high NO and low NAD(P)H due to iron.