• Molecular & Cellular Toxicology
• /
• 제4권3호
• /
• pp.246-252
• /
• 2008

Tumor tissue is usually contaminated by normal tissue components, which reduces the sensitivity of analysis for exploring genetic alterations. Although microdissection has been adopted to minimize the contamination of tumor DNA with normal cell components, there is a concern over the amount of microdissected DNA not enough to be applied to array-CGH reaction. To amplify the extracted DNA, several whole genome amplification (WGA) methods have been developed, but objective comparison of the array-CGH outputs using different types of WGA methods is still scarce. In this study, we compared the performance of non-amplified microdissected DNA and DNA amplified in 2 WGA methods such as degenerative oligonucleotide primed (DOP)-PCR, and multiple strand displacement amplification (MDA) using Phi 29 DNA polymerase. Genomic DNA was also used to make a comparison. We applied those 4 DNAs to whole genome BAC array to compare the false positive detection rate (FPDR) and sensitivity in detecting copy number alterations under the same hybridization condition. As a result microdissected DNA method showed the lowest FPDR and the highest sensitivity. Among WGA methods, DOP-PCR amplified DNA showed better sensitivity but similar FPDR to MDA-amplified method. These results demonstrate the advantage and applicability of microdissection for array-CGH analysis, and provide useful information for choosing amplification methods to study copy number alterations, especially based on precancerous and microscopically invaded lesions.

• 대기
• /
• 제17권1호
• /
• pp.69-86
• /
• 2007

The characteristics of the dual-Doppler wind retrieval method based on a three dimensional variational (3DVAR) conception were investigated from the following four points of view; the sensitivity of the number of iteration, the effect of the weak constraint term, the effect of the smoothness term, and the sensitivity of the error mixing ratio of the radial velocities. In the experiment, the radial velocities relative to the Gosan and Jindo radar sites of the Korea Meteorological Administration (KMA) were calculated from the forecasting of the WRF (Weather Research and Forecast; Skamarock, 2004) model at 1330 UTC 30 June 2006, which is the one and half hour forecast from the initial time, 1200 UTC on that day. The results showed that the retrieval performance of the horizontal wind field was robust, but that of the vertical wind was sensitive to the external conditions, such as iteration number and the on/off of the weak constraint term. The sensitivity of error mixing ratio was so large that even the horizontal wind retrieval efficiency was reduced a lot. But the sensitivity of the smooth term was not so large. When we applied this method to the real mesoscale convective system (MCS) between the Gosan and Jindo radar pair at 1430 UTC 30 June 2006, the wind structure of the convective cells in the MCS was consistently retrieved relative to the reflectivity factor structure. By comparing the vertical wind structure of this case with that of 10 minutes after, 1440 UTC 30 June 2006, we got the physical consistency of our method.

• 한국의류학회지
• /
• 제41권5호
• /
• pp.872-882
• /
• 2017

There are no specific guidelines on how to control the heat input for the heat generating smart base layer. This study investigated the mode of actuating heat pad attached to the base layer by performing a human wear test in a cold environment. Subjects participating in the test wore T-shirts, jumper and pants on the base layer with heating pads. Skin temperature, total time of heating and the number of switching for the heating mode were observed as the subject controlled the heating mode voluntarily. The comfortable range of skin temperature on the abdomen was larger than on the lower back. The subject felt hot and turned off the switch when the mean skin temperature of the abdomen was $48.8^{\circ}C$ and the lower back was $40.1^{\circ}C$. The total heating time and the number of actuating switching were larger for women than men. The voluntary action of heating for men with high cold sensitivity was significantly different from men with low cold sensitivity. Therefore, it is necessary (depending on gender and cold sensitivity) to set the heating mode differently for the automatic heat control of a future smart base layer.

• 대한수의학회지
• /
• 제48권4호
• /
• pp.457-462
• /
• 2008

The objective of this study was to determine appropriate sample size that simulated different assumptions for diagnostic test characteristics and true prevalences when designing serological surveillance plan for pullorum disease and fowl typhoid in domestic poultry production. The number of flocks and total number of chickens to be sampled was obtained to provide 95% confidence of detecting at least one infected flock, taking imperfect diagnostic tests into account. Due to lack of reliable data, within infected flock prevalence (WFP) was assumed to follow minimum 1%, most likely 5% and maximum 9% and true flock prevalence of 0.1%, 0.5% and 1% in order. Sensitivity were modeled using the Pert distribution: minimum 75%, most likely 80% and maximum 90% for plate agglutination test and 80%, 85%, and 90% for ELISA test. Similarly, the specificity was modeled 85%, 90%, 95% for plate agglutination test and 90%, 95%, 99% for ELISA test. In accordance with the current regulation, flock-level test characteristics calculated assuming that 30 samples are taken from per flock. The model showed that the current 112,000 annual number of testing plan which is based on random selection of flocks is far beyond the sample size estimated in this study. The sample size was further reduced with increased sensitivity and specificity of the test and decreased WFP. The effect of increasing samples per flock on total sample size to be sampled and optimal combination of sensitivity and specificity of the test for the purpose of the surveillance is discussed regarding cost.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권5호
• /
• pp.3237-3241
• /
• 2013

Background: Under-ascertainment and over-ascertainment are common phenomena in surveillance and registry systems of health-related events. Capture-recapture is one of the methods which is applied to determine the sensitivity of surveillance or registry systems to recognize cancer cases. This study aimed to estimate the number of cancers using data available both in the Cancer Registry Center of Northwestern Iran and in the Population-based Cancer Registry Center of Iran. Material and Methods: The studied population consisted of all cancerous cases in the northwest of Iran from 2008 to 2010. All data were extracted from two resources and entered into Microsoft Excel software. After removing common and repeat cases the data were statistically analyzed using a capture-recapture studies' specific software "CARE 1.4". Estimations were calculated by Chapman and Petersen methods with the approximate confidence interval of 95%. Results: From 2008 to 2010, the number of all cancer cases was estimated to be 21,652 (CI 95%: 19,863-22,101). Sensitivity rate of all cancer cases was 83.9% and that of Population-based Cancer Registry Center of Iran was 52%. It was 93.1% considering both resources. Conclusion: Using two resources and the capture-recapture method rather than a single resource may be a more reliable method to estimate the number of cancer cases.

• Journal of applied mathematics & informatics
• /
• 제31권5_6호
• /
• pp.747-767
• /
• 2013

In this paper we have constructed a mathematical model of alcohol abuse which consists of four compartments corresponding to four population classes, namely, moderate and occasional drinkers, heavy drinkers, drinkers in treatment and temporarily recovered class. Basic reproduction number $R_0$ has been determined and sensitivity analysis of $R_0$ indicates that ${\beta}1$ (the transmission coefficient from moderate and occasional drinker to heavy drinker) is the most useful parameter for preventing drinking habit. Stability analysis of the model is made using the basic reproduction number. The model is locally asymptotically stable at disease free or problem free equilibrium (DFE) $E_0$ when $R_0<1$. It is found that, when $R_0=1$, a backward bifurcation can occur and when $R_0>1$, the endemic equilibrium $E^*$ becomes stable. Further analysis gives the global asymptotic stability of DFE under some conditions. Our important analytical findings are illustrated through computer simulation. Epidemiological implications of our analytical findings are addressed critically.

• 한국농공학회논문집
• /
• 제48권6호
• /
• pp.31-41
• /
• 2006

The analysis used in this work was cost-benefit analysis method. All future costs and returns of a given mushroom house were discounted to the time of initial investment (present) by means of 3.5% discount rate. Then the cost of ownership was compared to the return from the system. This analysis method has been developed and coded into a balance sheet for use on a EXCEL program. Using this programmed analysis,a large number of the case studies were examined using different combinations of economic conditions. These results will be very useful to individuals considering investment in a mushroom house, or any similar production system. By the way of the sensitivity analysis for each important parameter, the change of the marginal cost-benefit period could be finally determined. These parameters were typically construction cost of mushroom house, cost of cooling system, required cooling and heating energy amounts, unit price of mushroom media bottle, growing number of media bottles, production weight per unit bottle, sale price of mushroom, and annual number of growing period, etc.

• Nuclear Engineering and Technology
• /
• 제56권6호
• /
• pp.2266-2273
• /
• 2024

A machine learning platform is proposed for the diagnosis of a severe accident progression in a nuclear power plant. To predict the key parameters for accident management including lost signals, a long short term memory (LSTM) network is proposed, where multiple accident scenarios are used for training. Training and test data were produced by MELCOR simulation of the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident at unit 3. Feature variables were selected among plant parameters, where the importance ranking was determined by a recursive feature elimination technique using RandomForestRegressor. To answer the question of whether a reduced order ML model could predict the complex transient response, we performed a systematic sensitivity study for the choices of target variables, the combination of training and test data, the number of feature variables, and the number of neurons to evaluate the performance of the proposed ML platform. The number of sensitivity cases was chosen to guarantee a 95 % tolerance limit with a 95 % confidence level based on Wilks' formula to quantify the uncertainty of predictions. The results of investigations indicate that the proposed ML platform consistently predicts the target variable. The median and mean predictions were close to the true value.

• 한국수자원학회논문집
• /
• 제39권8호
• /
• pp.677-690
• /
• 2006

본 연구에서는 LH-OAT (Latin Hypercube Ore factor At a Time) 민감도분석 방법과 SCE-UA (Shuffled Complex Evolution at University of Arizona) 최적화 기법을 적용하여 보청천 유역에서 SWAT모형에 대한 자동보정 방법을 제시하였다. LH-OAT 방법은 전역 민감도분석과 부분 민감도 분석의 장점을 조합하여 가용매개변수 공간에 대하여 효율적으로 매개변수의 민감도 분석이 가능하게 하였다. LH-OAT민감도 분석으로부터 결정된 매개변수의 민감도 등급은 SWAT 모형의 자동보정 과정에서 요구되는 보정대상 매개변수의 선택에 유용하게 적용될 수 있다. SCE-UA 방법을 적용한 SWAT모형의 자동보정 해석결과는 보정자료, 보정매개변수, 통계적 오차의 선택에 따라서 모형의 성능이 좌우되었다. 보정기간과 보정매개변수가 증가함에 따라 검증기간에 대한 RMSE (Root Mean Square Error), NSEF (Nash-Sutcliffe Model Efficiency), RMAE (Relative Mean Absolute Error), NMSE (Normalized Mean Square Error) 등의 모형오차는 감소하였지만, NAE (Normalized Average Error) 및 SDR(Standard Deviation Ratio)은 개선되지 않았다. SWAT모형의 보정에 적용되는 보정자료, 보정매개변수 및 모형평가를 위한 통계적 오차 선택이 해석결과에 미치는 복잡한 영향을 이해하기 위하여 다양한 대표유역을 대상으로 추가적인 연구가 필요하다.

• 한국군사과학기술학회지
• /
• 제11권2호
• /
• pp.109-115
• /
• 2008

A Plastic Bonded Explosive(PBX) is mainly composed of nitramine explosive and polymer binder. The great number of serious applications of PBX requires the good adhesion between nitramine crystals and binder, which depends on the surface characteristics of a filler and binder. In the pursuit of the better design to achieve the enhanced adhesion, profound knowledge of the surface and interfacial characteristics of explosive and binder should be exploited. In this study, the influences of physicochemical properties between RDX and binders such as interfacial tension($\gamma_{SL}$), latent heat($Q_m$), and density($\rho$) on impact sensitivity of PBX were investigated. As experimental results, the major contribution factor to impact sensitivity of PBX was the interfacial tension, compare with other surface properties. The correlation coefficient of $H_{50}$ versus $\gamma_{SL}$ is 0.9932 when a polynomial regression method was used.