• Korean Circulation Journal
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• 제53권6호
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• pp.367-386
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• 2023

Ischemic heart disease remains the primary cause of morbidity and mortality worldwide. Despite significant advancements in pharmacological and revascularization techniques in the late 20th century, heart failure prevalence after myocardial infarction has gradually increased over the last 2 decades. After ischemic injury, pathological remodeling results in cardiomyocytes (CMs) loss and fibrosis, which leads to impaired heart function. Unfortunately, there are no clinical therapies to regenerate CMs to date, and the adult heart's limited turnover rate of CMs hinders its ability to self-regenerate. In this review, we present novel therapeutic strategies to regenerate injured myocardium, including (1) reconstruction of cardiac niche microenvironment, (2) recruitment of functional CMs by promoting their proliferation or differentiation, and (3) organizing 3-dimensional tissue construct beyond the CMs. Additionally, we highlight recent mechanistic insights that govern these strategies and identify current challenges in translating these approaches to human patients.

• 기록학연구
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• 제37호
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• pp.3-39
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• 2013

이 논문은 공동체 아카이브가 가장 활발하게 구축되고 있는 영국의 공동체 아카이브 운동의 현 단계를 분석하고 이로부터 국내 공동체 아카이브 발전을 위한 함의를 도출하는데 목적이 있다. 영국의 '독립' 공동체 아카이브는 풀뿌리운동의 일환으로서 공동체 구성원의 아래로부터의 참여, 자율적 통제, 내적 소유권이 요체라 볼 수 있다. 영국은 역사적으로 1960년대에 등장한 공동체 개념으로 시작해 최근 도시재생프로젝트를 거치며 공동체 아카이브가 활성화되는 시기들을 지났다. 최근에는 주류 아카이브 등 공적 영역과의 파트너십에 의해 공동체 아카이브가 거버넌스(협치) 단계에 이르고 있다. 무엇보다 영국형 공동체 아카이브는, 공동체의 내재적 발전의 산물이며 자율적 통제에 기반하여 '그들만의 방식'으로 아카이브를 구축해왔다. 이러한 영국형 공동체 아카이브의 성격은 최근 국내 '마을' 공동체 아카이브 운동과 그 기본적 성격 면에서 많이 닮아 있다. 하지만 영국형 아카이브 모델은, 주류 아카이브에 포획되거나 주도되는 방식이 아니라 공동체 아카이브의 자율성을 기본으로 하면서도 공적 영역의 노하우를 끌어들이며 거버넌스(협치)를 구성하는 방식으로 진화하고 있다. 개방과 협업의 공동체 아카이브 구축을 위한 방법론 모색이 절실한 국내 기록학계에 영국의 최근 변화는 시사하는 바가 크다.

• Journal of Plant Biotechnology
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• 제41권3호
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• pp.116-122
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• 2014

다양한 형태적 특징을 가진 배추 유전자원의 소포자 배양을 통한 고정계통의 육성 및 효율적인 소포자 배 발생조건을 확립시키기 위하여 50종의 배추 유전자원을 대상으로 소포자 배양을 실시하여 30종에서 반수체배의 생산이 확인되었다. 소포자 배 발생률은 순무형인 IT135449에서 가장 높았고 일부 유지작물형(winter oil)에서는 배 발생이 관찰되지 않았다. 소포자 배양을 통한 다양한 배추 유전자원의 배 발생 효율의 증대를 위하여 유전자원 4종(IT135449, IT199710, IT212886, IT218043)을 대상으로 고온처리 기간에 따른 소포자 배 발생 효율을 확인한 결과, 3종에서는 $32^{\circ}C$에서 2일간 고온처리 할 경우 배 발생률이 가장 높았고 summer oil형 IT218043에서는 3일간 처리할 경우 가장 높은 배 발생률이 관찰되었다. 또한 소포자배의 식물체 재분화에 영향을 미치는 식물 생장조절제의 농도를 조사한 결과, 결구배추형인 IT199710에서는 NAA $0.5mg{\cdot}L^{-1}$과 BAP $1mg{\cdot}L^{-1}$를 첨가한 처리구에서 가장 높은 재분화율이 관찰되었으나 순무형인 IT135449와 청경채형 IT212886에서는 생장조절제 무처리구에서 가장 높은 재분화율이 관찰되었다. 소포자 배 유래 재분화 식물체들을 대상으로 배수성 검증을 실시한 결과 종자를 형성한 모든 식물이 자연배가를 통한 이배체 식물임이 확인되었다. 본 실험의 결과는 다양한 배추 유전자원의 소포자 배양효율 증진을 위해 이용될 수 있을 뿐 아니라 획득한 고정 계통은 이후 배추의 육종 및 유전자 기능 연구의 소재로 활용될 수 있을 것이다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권6호
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• pp.588-593
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• 2008

There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and $PPAR{\gamma}2$ and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.

• 한국어병학회지
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• 제20권3호
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• pp.307-313
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• 2007

In this study, a QCM biosensor was made to detect Edwardsiella tarda (E. tarda) using a specific antibody. A 9 MHz AT-cut piezoelectric wafer layered with two gold electrodes of 5mm diameter had a reproducibility of 0.1 Hz in frequency response and was used as the transducer of the QCM biosensor. Self assembled layer (SAM) was conformed on a quartz crystal by treating with 3-mer-captopropionic acid (MPA) and activated with N-ethyl-N'-(3-dimethyl-aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The resulting NHS group was further converted to hydrazide by the reaction with hydrazine. Aldehyde group was introduced into the carbohydrate moiety of anti-E. tarda antibody by the reaction with periodic acid and was used to immobilise the antibody through the reaction with hydrazide group on the electrode surface. A baseline was established in the presence of phosphate-buffered saline (PBS) and a resonant frequency (F1) was measured. Sample was added to the sensor surface and second resonant frequency (F2) was measured after unbound substances were washed out with PBS several times. Finally, the frequency shift (ΔF) representing the mass change was calculated by subtracting F2 from F1. After adding the oxidized anti-E. tarda antibody to the electrode surface containing hydrazide group, frequency shift of 288.811.4 Hz (mean S.E) was observed, thus proving that considerable amount of antibody was immobilized. In the immunoassay test, the frequency shift of 1877.75 Hz, 580.67 Hz, 221.39 Hz, 7.671.83 Hz (mean S.E) were observed at doses of 1000, 500, 100, 50 g of bacterial cells, respectively. It was also demonstrated that the prepared sensor chip was stable enough to withstand repeated surface regeneration with 0.2 M Tris-glycine and 1 % DMSO, pH 2.3 more than ten times.

• 한국콘텐츠학회논문지
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• 제10권6호
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• pp.297-306
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• 2010

도시는 다양한 역사적 경험과 기억을 간직한 공간이다. 이러한 공간에서 간직해온 다양한 사회 문화적 환경을 통해 지역발전을 위한 일련의 활성화 정책들이 등장하기 시작하고 이 과정에서 지역들이 갖고 있는 고유의 역사와 문화는 훌륭한 핵심수단으로 인식되기 시작했다. 도시가 경쟁력을 갖기 위해 그 도심만의 유 무형의 자원을 개발하여 이를 매력적인 자산으로 활용하는 것이 그 출발점이라 할 수 있다. 그 지역이 갖는 도시정체성을 확립하고 이를 위해 특별한 장소 만들기를 위한 계기가 필요하다. 이러한 도시 정체성은 그 도시가 지금까지 지내온 시간을 바탕으로 한다. 이러한 역사적인 요소를 통해 지역의 문화적 역사적 도시 이미지를 제고시키는 전략을 개발하여 대외적인 홍보방안 수립을 위한 도시 마케팅적 프로그램을 제안하고 한다. 대전시를 비롯하여 국내외에 존재하는 문화적 요소인 근대 역사물의 보존과 활용을 통해 지역의 매력적인 도시로서 이미지를 제고 시키고 도시 정체성에 대한 포지셔닝을 구축하고, 지속가능한 도시 재생을 통한 도시 마케팅 활용에 대한 시사점을 제공하고자 한다.

• Journal of Periodontal and Implant Science
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• 제48권2호
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• pp.114-123
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• 2018

Purpose: The aim of the current study was to develop a computer-assisted detection system based on a deep convolutional neural network (CNN) algorithm and to evaluate the potential usefulness and accuracy of this system for the diagnosis and prediction of periodontally compromised teeth (PCT). Methods: Combining pretrained deep CNN architecture and a self-trained network, periapical radiographic images were used to determine the optimal CNN algorithm and weights. The diagnostic and predictive accuracy, sensitivity, specificity, positive predictive value, negative predictive value, receiver operating characteristic (ROC) curve, area under the ROC curve, confusion matrix, and 95% confidence intervals (CIs) were calculated using our deep CNN algorithm, based on a Keras framework in Python. Results: The periapical radiographic dataset was split into training (n=1,044), validation (n=348), and test (n=348) datasets. With the deep learning algorithm, the diagnostic accuracy for PCT was 81.0% for premolars and 76.7% for molars. Using 64 premolars and 64 molars that were clinically diagnosed as severe PCT, the accuracy of predicting extraction was 82.8% (95% CI, 70.1%-91.2%) for premolars and 73.4% (95% CI, 59.9%-84.0%) for molars. Conclusions: We demonstrated that the deep CNN algorithm was useful for assessing the diagnosis and predictability of PCT. Therefore, with further optimization of the PCT dataset and improvements in the algorithm, a computer-aided detection system can be expected to become an effective and efficient method of diagnosing and predicting PCT.

• Archives of Plastic Surgery
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• 제40권6호
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• pp.666-675
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• 2013

Background Stem cells are a unique cell population characterized by self-renewal and cellular differentiation capabilities. These characteristics, among other traits, make them an attractive option for regenerative treatments of tissues defects and for aesthetic procedures in plastic surgery. As research regarding the isolation, culture and behavior of stem cells has progressed, stem cells, particularly adult stem cells, have shown promising results in both translational and clinical applications. Methods The purpose of this review is to evaluate the applications of stem cells in the plastic surgery literature, with particular focus on the advances and limitations of current stem cell therapies. Different key areas amenable to stem cell therapy are addressed in the literature review; these include regeneration of soft tissue, bone, cartilage, and peripheral nerves, as well as wound healing and skin aging. Results The reviewed studies demonstrate promising results, with favorable outcomes and minimal complications in the cited cases. In particular, adipose tissue derived stem cell (ADSC) transplants appear to provide effective treatment options for bony and soft tissue defects, and non-healing wounds. ADSCs have also been shown to be useful in aesthetic surgery. Conclusions Further studies involving both the basic and clinical science aspects of stem cell therapies are warranted. In particular, the mechanism of action of stem cells, their interactions with the surrounding microenvironment and their long-term fate require further elucidation. Larger randomized trials are also necessary to demonstrate the continued safety of transplanted stem cells as well as the efficacy of cellular therapies in comparison to the current standards of care.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2012년도 추계총회 및 학술대회 논문집
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• pp.4-4
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• 2012

Proteins enable biology to be viable through molecular interactions (such as in antigen-antibody, ligand-receptor, and drug-target) with

• 교육시설 논문지
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• 제22권1호
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• pp.13-23
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• 2015

Currently, the group study becomes the most popular method and common custom for learning in public that is distinctly different from the preferred way of self-study in the last decades. As a result, the college and public facilities have been significantly changed to provide more and more new spaces for discussion, learning and sharing information with others as one of the remarkable improvements and advantages. On the other hands, library is one of the all-important public sites to obtain information and knowledge for students. Moreover, we can split out an individual space from the reading rooms to take part in the group activities such as group meetings and academic exchanges. Recently, several universities begin to recognize the value of group study and try to meet the research needs. Also, needs for students, and the group study rooms are applied into the new buildings and rebuilt ones under this background. In this study, It focuses on analyzing of the 134 group study rooms to investigate the variations in universities and classify the types of buildings with site researches through 14 indicators of indoor environments. To investigate different types of group study rooms, we use the SD method to analyze the findings. So far there is no research focusing on the study rooms, especially for the analysis of their types and indoor environments features. Therefore, this article can provide a theoretical basis and evidence to related researches; also can help us to improve indoor environments to offer a better learning environments for the students in the future.