• 여성건강간호학회지
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• 제15권1호
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• pp.43-53
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• 2009

Purpose: This study was to identify what experience meant for the wives that attempted IVF-ET(In Vitro Fertilization-Embryo Transfer) program due to the spouse's infertility and provide fundamental materials to improve nursing interventions. Method: Giorgi's phenomenological method was employed, the subjects were five wives who had ever attempted IVF-ET program due to the infertility of their spouses. In-depth interview and observation method were utilized to gather information from April to December 2003. Result: The significant results from analyzing the interviews can be grouped into 34 themes, 8 categories. The essential themes for the experiences of the wives were 'shocked by the unbelievable reality', 'can't give up the connection to the blood', 'Lack of social education on pregnancy, and childbirth', 'self-pity', 'feelings of both families', 'Being afraid of the unexpected result', 'physical and mental agony', 'Positively coping with the reality. Conclusion: The results show that infertility is not a mere personal matter, and infertile people, their families and society should team up with in tackling it. The physical, psychological and social problems triggered by infertility could be ironed out by making both personal and collaborative approaches to that.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.345-351
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• 2013

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: $99.5{\pm}0.8%$, DMSO 7.5%: $99.5{\pm}0.7%$, and DMSO 10.0%: $99.6{\pm}0.6%$). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: $96.2{\pm}2.3%$, DMSO 7.5%: $95.3{\pm}3.6%$, 10.0%: $96.6{\pm}1.8%$) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm ($98.4%{\pm}0.5$) and DMSO concentration level of 5.0% ($97.8{\pm}0.1%$) were not significantly different. However, with 7.5% ($97.2{\pm}0.6%$) and 10.0% DMSO concentrations ($95.9{\pm}0.2%$) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; $66.8{\pm}1.8%$, HR: $82.0{\pm}12.9%$) and 2 years (FR; $78.5{\pm}14.8%$, HR: $79.3{\pm}0.6%$) cryopreserved sperm were lower than control (FR; 100%, HR: $91.1{\pm}3.6%$) and 2 days cryopreserved sperm (FR; $99.6{\pm}0.6%$, HR: $96.6{\pm}1.8%$). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

• 한국환경농학회지
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• 제41권4호
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• pp.230-235
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• 2022

BACKGROUND: Ammonia gas emitted from nitrogen fertilizers applied in agricultural land is an environmental pollutant that catalyzes the formation of fine particulate matter (PM2.5). A significant portion (12-18%) of nitrogen fertilizer input for crop cultivation is emitted to the atmosphere as ammonia gas, a loss form of nitrogen fertilizer in agricultural land. The widely practiced method for fertilizer use in agricultural fields involves spraying the fertilizers on the surface of farmlands and mixing those with the soils through such means as rotary work. To test the potential reduction of ammonia emission by nitrogen fertilizers from the soil surface, we have added N, P, and K at 2 g each to the glass greenhouse soil, and the ammonia emission was analyzed. METHODS AND RESULTS: The treatment consisted of non-fertilization, surface spray (conventional fertilization), and soil depth spray at 10, 15, 20, 25, and 30 cm. Ammonia was collected using a self-manufactured vertical wind tunnel chamber, and it was quantified by the indophenol-blue method. As a result of analyzing ammonia emission after fertilizer treatments by soil depth, ammonia was emitted by the surface spray treatment immediately after spraying the fertilizer in the paddy soil, with no ammonia emission occurring at a soil depth of 10 cm to 30 cm. In the upland soil, ammonia was emitted by the surface spray treatment after 2 days of treatment, and there was no ammonia emission at a soil depth of 15 cm to 30 cm. Lettuce and Chinese cabbage treated with fertilizer at depths of 20 cm and 30 cm showed increases of fresh weight and nutrient and potassium contents. CONCLUSION(S): In conclusion, rather than the current fertilization method of spraying and mixing the fertilizers on the soil surface, deep placement of the nitrogen fertilizer in the soil at 10 cm or more in paddy fields and 15 cm or more in upland fields was considered as a better fertilization method to reduce ammonia emission.

• 한국육종학회지
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• 제41권4호
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• pp.397-402
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• 2009

Self-incompatibility (SI) prevents self-fertilization by inhibiting the pollen tube growth of self-pollen. Molecular analysis has revealed that the S locus comprises a number of genes, such as the S-locus glycoprotein (SLG), the S-locus receptor kinase (SRK), and SP11 (SCR). Although molecular markers related to those genes have been developed, a simple S-haplotype detecting method has not been reported due to the highly polymorphic and relatively small coding regions. In this study, the sequence characterized amplified region (SCAR) markers were used to establish an efficient radish genotyping method. We identified the S-haplotypes of 192 radish accessions using 19 different markers, which proved to be highly reliable. The accessions were assigned to 17 types of S-haplotypes, including 8 types of SRKs and 9 types of SLGs. Since the developed SCAR markers are based on their gene sequences, we could easily identify the S-haplotypes by a single specific band, with the highest frequencies detected for SLG 5, SRK 1, and SLG 1, in order. Among the tested markers, the SLG 1, SRK 1, and SRK 5 markers exhibited high reliability, compared to phenotypic results. Furthermore, we identified the seven types of unreported SLGs using SLG Class -I and -II specific markers. Although the developed SCAR markers still need to be improved for the genotyping of all S-haplotypes, these markers could be helpful for monitoring inbred lines, and for developing the MAS in radish breeding programs.

• 현장농수산연구지
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• 제23권2호
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• pp.25-32
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• 2021

우리나라에서 재배되고 있는 백합 구근의 대부분을 수입에 의존하고 있어 국내 품종을 개발하여 보급할 필요가 있다. 분화용 나팔나리 유전자원 'Ace', 'Nelli White'를 수집하여 1996년부터 2010년까지 자가수정을 실시하였다. 2012년에 자가수정 7세대 'L₂-17-4' 과 'L₂-16-6' 계통을 교잡하여 1대잡종 나팔나리 'Bright LoSong'를 개발하였다. 'Bright LoSong'은 균일성 및 지역성응성 검정은 바탕으로 2014년부터 2015년까지의 품평회를 통하여 선발하여 품종 등록은 2017년에 되였다. 분화용 나팔나리 일대잡종 'Bright LoSong'는 백색의 나팔모양의 홑꽃으로 반점이 없으며, 식물체 당 개화수가 5.8개로 많은 편이며, 꽃의 직경은 9.8cm이고 측향으로 개화한다. 초장은 38.4cm이고, 잎의 수는 30.8개이고, 잎의 길이는 14.9cm이며, 잎의 폭은 1.9cm이다. 일대잡종 'Bright LoSong'는 대조품종인 'Mount Carmel' 보다 화수가 많아서 개화 기간이 길며 소비자 선호도가 높다. 일대 잡종 'Bright LoSong'은 실내에서 재배가 원활하여 분화용으로 선호도가 우수하며 양친을 순계화하여 육성하였기 때문에 품종의 재현이 가능하다.

• 자연과학논문집
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• 제11권1호
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• pp.95-98
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• 1999

$CO_2$ gas 처리가 배추 자가불화합성 타파에 미치는 영향을 조사하고자 배추 inbred line의 암술에서 mRNA를 분리하여 DD-PCR을 수행하였다. $CO_2$ gas를 처리한 암술에서 분리한 mRNA와 처리하지 않은 꽃에서 분리한 암술 mRNA 발현과의 특이적인 차이를 DD-PCR로 조사한 결과 세 가지 각기 다른 anchor primer에 대해 모두 18개의 특이적 증폭 DNA fragment를 얻을 수 있었다. 이 결과로 미루어 보아 $CO_2$ gas 처리에 의한 암술 mRNA발현의 변화가 배추 자가불화합성 타파에 영향을 미치는 것으로 생각된다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.1395-1404
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• 1993

Application of water-saving self-feeders can reduce water consumption of pigs by more than 50% . so the feeding-watering system one of the most important way of the reduction of the slurry. Bioactive deep litter housing can eliminate slurry. Matured urine, faeces and litter can use for the purposes of soil conditioning and fertilizing . Water-saving slurry handling technology can halve manure dilution so it can double the nutrient content of the slurry. By using of straw bale biofilter for reducing emissions of pig houses makes fattening of pigs possible close to populated area. Developed rate control system for slurry application make avoiding over-fertilization possible , can fulfill better the demand of nutrient of plants. By means of computer aided manure utilization system area distribution of soil characteristics can determinate . The system is suitable for planning the utilization of manure and slurry in environment -friendly way.

• Journal of Plant Biology
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• 제26권3호
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• pp.141-150
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• 1983

Antithamnion sparsum Tokida isolated from the southern and western coasts of Korea was investigated in culture, comparing the morphological character with A. defectum Kylin from the Pacific North America. A. sparsum basically showed a Polysiphonia-type life history. However, it sometimes exhibited a monoecious reproducton and the carpospores released from the cystocarp by self-fertilization unexpectedly developed into the plants bearing spermatangia alone. These male plants were not functional up to 60 days culture. The results of intraspecific crosses between populations of A. sparsum were positive and the hybrid carpospores gave rise to normal tetrasporophytes. On the other hand, theinterspecific crosses between A. sparsum and A. defectum were successful partly, evidenced by the gonimoblast development and the release of carpospores in case of A. sparsum (male)$\times$A. defectum (female), but not in case of A. sparsum (female)$\times$A. defectum (male). Thus, the both species were still under the speciation.

• Journal of Ecology and Environment
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• 제30권3호
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• pp.225-229
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• 2007

The mating systems of natural populations of Potentilla freyniana in Korea were determined using allozyme analysis. The result suggests that P. freyniana is outcrossing as well as employing vegetative reproduction by stolon (self-fertilization rate, s < 0.5). The values of the inbreeding coefficient of eight populations in Korea varied from 0.244 to 0.331, with an average value of 0.274. For eight natural populations, multi-locus estimates of outcrossing (tm) was 0.603 across 13 polymorphic loci, with individual population values ranging from 0.530 to 0.652. The relatively low outcrossing rates of some populations could be attributed to extensive vegetative reproduction by stolon and the isolation of flowering mature plants. Although P. freyniana usually propagated by asexually-produced ramets, I could not rule out the possibility that sexual reproduction occurred at a low rate because each ramet may produce terminal flowers. Although heterozygote excess was observed in some natural populations, most populations exhibited varying degrees of inbreeding and a heterozygote deficit.

• 지역사회간호학회지
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• 제4권2호
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• pp.170-187
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• 1993

The natural growth rate of the Korean population has decreased from 3.0% in 1960 to 1.0% in 1990. This was done with family planning program which was introduced by the government in 1961. The family planning program focused on birth control rather than the characteristics of the individuals and motivations of contraception. People were simply forced to use the method. Whereas, Natural Contraceptive is a method of family planning based solely on the timing of intercourse with the naturally occurring' physiological manifestation of fertilization and in fertilization during the menstrual cycle. This is the combination of self fertility awareness with periodic abstinence. Natural family .planning(NFP) programs in Korea were first started in the Chun-Chen diocese of catholic church by Bishop Thomas Stewart in 1970 In 1975, the Bishops conference launched the Korea Happy Family Movement in the Catholic Hospital Association, to promote the natural family planning. An average of 70,000 people, including adolescents, college students, unmarried and married persons, arid the clergies were trained during a six-year period (1986-1991). 61.5%(24,542 people) of those who completed 3 cycles during 6 year period (1986-1991) became autonomous users and the range was from 48.1% to 78.2%. In 1986, 22.7% of NFP individuals who drooped out of the program because of the desire for conception (23.4%), the difficulty of the method used(25.8%), and the loss of interest(22.8%). During the six-year period the unplanned pregnancy rate at the NFP was 2.9%. The range of the pregnancy rate was at 1.2-9.8%. The rate was decreased as years passed. The major reason for the failure of contraceptive was error by the individuals(61.1%). The percentage of the success of conception was 18.1% of 2.979 for achieving pregnancy. The highest percentage was 58.2% (99 users) in Kwang-Joo diocese and next was 37.1% (10 users) in Chong Joo diocese.