• Asian Pacific Journal of Cancer Prevention
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• v.13 no.3
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• pp.761-766
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• 2012

Objective: Although various human cancer stem cells (CSCs) have been defined, their applications are restricted to immunocompromised models. Developing a novel CSC model which could be used in immunocompetent or transgenic mice is essential for further understanding of the biomolecular characteristics of tumor stem cells. Therefore, in this study, we analyzed murine lung cancer cells for the presence of CSCs. Methods: Side population (SP) cells were isolated by fluorescence activated cell sorting, followed by serum-free medium (SFM) culture, using Lewis lung carcinoma cell (LLC) line. The self-renewal, differentiated progeny, chemosensitivity, and tumorigenic properties in SP and non-SP cells were investigated through in vitro culture and in vivo serial transplantation. Differential expression profiles of stem cell markers were examined by RT-PCR. Results: The SP cell fraction comprised 1.1% of the total LLC population. SP cells were available to grow in SFM, and had significantly enhanced capacity for cell proliferation and colony formation. They were also more resistant to cisplatin in comparison to non-SP cells, and displayed increased tumorigenic ability. Moreover, SP cells showed higher mRNA expression of Oct-4, ABCG2, and CD44. Conclusion: We identified SP cells from a murine lung carcinoma, which possess well-known characteristics of CSCs. Our study established a useful model that should allow investigation of the biological features and pharmacosensitivity of lung CSCs, both in vitro and in syngeneic immunocompetent or transgenic/knockout mice.

• The Research Journal of the Costume Culture
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• v.22 no.5
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• pp.760-775
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• 2014

In the era of 2.0 web, blog has become the media that men can express themselves with fashion more actively and independently, as paying much attention to their personal appearance and cultivating an upscale lifestyle. They often create their fashion images in the virtual space where enables a free and creative operations of self-expression. The study aims to identify the types of men's ego-images represented on the personal fashion blogs based on the framework of analysis from the previous research (Suh, 2014), to build the base data for analyzing men's fashion style in $21^{st}$21st century that reflects changes in men's sexual images, and to verify the framework as comparing with the previous case study about the women blogs (Suh, 2014). The case studies conducted 5 men's personal blogs such as bryanboy, iamgala, little fashionisto, katelovesme, and stylentonic. The study results almost same types of women's ego-images as following. The imaginary ego-image is classified as narcissism, regression, identification, and virtuality, the social ego-image as symbolism of roles and others'desire, the real ego as primary instinct, practical reality, object a, jouissance and sexual perversion. The personal style of men shown on the fashion blogs appears as a significant factor to analyze male customers in the growing men's beauty and fashion market.

• Korean Journal of Community Nutrition
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• v.21 no.6
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• pp.509-519
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• 2016

Objectives: Adulterated food education in adolescence period is very important because dietary management related to food safety is not made in a short period. This study aimed to identify dietary lifestyle factors which drive adulterated food management among middle and high school students. Methods: Data was collected from 270 middle and high school students in Daegu using a self-administered questionnaire in March and April of 2015. Data was analyzed using frequency analysis, one-way analysis of variance, ${\chi}^2$-test, factor analysis, reliability analysis, regression analysis, and cluster analysis. Results: The results of factor analysis indicated that adulterated food management awareness was classified into necessity, difficulty, and food purchasing anxiety. The adulterated food management capability was sub-grouped into environmental grasp, food identification, cooking hygiene, and situation management. The adulterated food management efficacy composed of management confidence, action intention, and knowledge. Dietary lifestyle comprised of gustation, family, and health factors after factor analysis, and it consisted of all seeking group, gustation seeking group, family seeking group, health seeking group, and family and health seeking group after cluster analysis. The gustation, family and health factors were significantly affected the factors of awareness, capability and efficacy of adulterated food management (p < 0.05). The frequency of health conditions, helping with meal preparation, and the times of eating out were significantly different according to seeking groups of dietary lifestyle (p < 0.01). The scores of awareness, capability and efficacy of adulterated food management of family and health seeking group were significantly higher than the other seeking groups (p < 0.05). Conclusions: This study suggests that adulterated food management education programs should account for gustation, family and health factors of dietary lifestyle to be effective for adolescents.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.49-57
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• 2016

We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock 'Marubakaido' (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus ${\times}$ domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus ${\times}$ domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

• Journal of the Korea Safety Management & Science
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• v.22 no.1
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• pp.33-44
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• 2020

This study analyzed how the installation of a pressure gauge in the indoor fire hydrant of an apartment building affected identifying pressurized water in the pipe, making it easier to conduct internal inspection on the fire suppression system, and ensuring reliability of fire suppression. The following are the study's results: First, identifying pressurized water in the indoor firefighting pipe had a positive effect on the installation of a pressure gauge in the indoor fire hydrant. This implies that a higher level of identification of pressurized water in the indoor firefighting pipe had a positive impact on improving the installation and use of a pressure gauge in the indoor fire hydrant. Second, making it easier for the fire safety officer to inspect the fire suppression system had a positive effect on the installation of a pressure gauge in the indoor fire hydrant. This suggests that if it becomes easier for the apartment building's stakeholder to conduct internal inspection or the firefighting facility manager to carry out inspection on the fire suppression system, it would have a positive effect on the installation of a pressure gauge in the indoor fire hydrant. Finally, ensuring reliability in fire suppression had a positive effect on the installation of a pressure gauge in the indoor fire hydrant. This implies that if it becomes easier to identify pressurized water in the indoor firefighting pipe, for the fire safety officer to conduct internal inspection, or for the firefighting facility manager to carry out inspection in accordance with the fire suppression system's internal inspection requirements, it would increase reliability in fire suppression, making it more necessary to install a pressure gauge in the indoor fire hydrant.

• The Journal of the Korea Contents Association
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• v.12 no.2
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• pp.116-126
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• 2012

As a film maker, Lee Chang Dong stands out from most Korean film makers who work within the trappings of genre films. To be sure, Lee has also used the trappings of genre films, such as noire and melodrama, but primarily as a tool to communicate with film audience. In his most recent film "Poetry", Lee seems to have even stripped even the minimal trappings of genre film. Lee commands the audience to self-reflect and work towards their own conclusions by denying them the illusory identification on screen. In this way, Lee's works are counter cinema. Lee achieves a distancing effect using such filmic apparatuses as hand-held camera, fantasy, mise en abyme, and returned gaze. Through these filmic apparatuses, Lee exposes the re-presentation of text and compels his audience to see the historical and political contexts of the text. In this study, I make the case that Lee Chang Dong's film making is an act of repetition compulsion that cultivates ethical reflection, through symbolization of the invisible realities.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.307-309
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• 2013

Diligence/Indolence records are the basic data at a human resource management and the essential data for measure salaries of employees. However, since the D/I systems using RFIDs or biometric sensors require hish costs to build and operate the D/I systems, it is difficult for to small business or self-employed business to use them. In this paper, we propose the Diligence/Indolence system to use the location identification of a smart phon. To locate the position of an employee, the WiFi signal of a smart phone and the wireless access point are exploited. Because the pre-owned hardware resources are used and initial building costs are low, it is easy for small business to use the proposed D/I system with low cost.

• Korean Journal of Acupuncture
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• v.32 no.2
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• pp.66-74
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• 2015

Background : Saam acupuncture were composed by applying the elemental concepts from the Five Phase theory - the relationships between the cycles such as Saeng(Sheng, 'nourishing' or 'creating') and Geuk(Ke, 'suppressing' or 'controlling') - onto the Five Phase points and 12 channels to compensate for the imbalance in each of the 12 main energy traits. Objective : The present study is aimed to find out the characteristics of Five Phase points pattern in Saam acupuncture. Methods : We analysed the characteristics of five elements of the Five Phase points in Korean medical texts such as Saamdoinchimguyogyeol, Dongeuibogam and Chimgugyeongheombang in mid Chosun Dynasty. Using non-negative factorization(NNMF) methods, we extracted the feature matrix of five elements of Five Phase points in each classic medical text. Results : In Saam acupuncture, two characteristics were most prominent: (1) "Self" component of Five elements, (2) "Mother" and "Grandmother" component of Five elements. Conclusions : Saam acupuncture used the combination of Five-Shu acupoint based on ZangFu pattern identification. Our findings suggest that grasping the characteristics of Five Phase points combinations can improve the understanding the selection of the relevant acupoints based on the ZangFu pattern identifications.

• Journal of the Korean Home Economics Association
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• v.40 no.4
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• pp.201-210
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• 2002

The purpose of this study was to extend the social teaming and imitation theory in an attempt to examine the effect of star-entertainer imitation behavior on clothing behaviors in relation to adolescents' age and gender variables. The research was a survey and the subjects were 895 adolescents in Taejon, Korea. The measuring instruments was serif-administrated questionnaire consisted of star-entertainer imitation behaviors and clothing behaviors. The collected data were analyzed using factor analysis, ANOVA, Duncan's multiple range test. Results were as fellows: 1) The results of analysing the response of star-entertainer imitation behaviors showed that adolescents had very positive attitudes and high psychological need to identify self with star-entertainers, however, their actual identification behaviors were not very noticeable because of parents' restraint and school regulations on students' appearances and clothing behaviors. 2) The results of analysing the response of clothing behaviors showed four factors such as 'fashion$.$clothing interest', 'psychological dependency on clothing' , 'clothing exhibition', and 'clothing conformity'. 3) Star-entertainer imitation behaviors had significant effects on clothing behaviors; the group with more star-entertainer imitation behaviors showed more fashion$.$clothing interest, higher psychological dependency on clothing and clothing exhibition, and lower clothing conformity than the group with less star-entertainer imitation behaviors. 4) Clothing behaviors showed the differences according as adolescents' age and gender; the female adolescents showed more fashion$.$clothing interest and clothing exhibition than male, the high school and cortege age groups showed higher psychological dependency on clothing than other age groups. It is concluded that the results of this study support social teaming and imitation theory since TV media give strong influence on the TV viewers through presenting various modeling stimuli to adolescents' lives.

• Korean Journal of Health Education and Promotion
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• v.30 no.5
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• pp.113-124
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• 2013

Objectives: The objective of this study was developing a community-based HIV prevention program to enhance the safe sexual behavior among rural Ethiopian female youth. Methods: A community-based HIV prevention program was developed using the Cox's Interaction Model of Client Health Behavior. The program development was carried out in four phases using a mixed research method: need-assessment which consisted of three steps (secondary-data analysis, key-informant interview, and focused-group interview); identification of preliminary program contents; expert's review of the program contents for the validity and cultural acceptability of the program; and refinement of the proposed program contents. Results: The HIV prevention program developed in this study consisted of three modules; the first module was for enhancing the youth's self-esteem, the second was for providing the youth with information regarding HIV/AIDS and safe sexual behavior, and the third was for improving the youth's communication skills and refusing skills. Conclusions: The need assessment and expert's review was very effective way to reflect sociocultural factors of rural Ethiopia for developing HIV prevention program for female youth. Further research is desirable for verifying the effectiveness of the developedprogram.