• Biomolecules & Therapeutics
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• 제22권4호
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• pp.363-369
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• 2014

Synthetic cannabinoids (CBs) such as the JWH series have caused social problems concerning their abuse liability. Because the JWH series produces euphoric and hallucinogenic effects, they have been distributed illegally under street names such as "Spice" and "Smoke". Many countries including Korea have started to schedule some of the JWH series compounds as controlled substances, but there are a number of JWH series chemicals that remain uncontrolled by law. In this study, three synthetic CBs with different binding affinities to the $CB_1$ receptor (JWH-073, 081, and 210) and ${\Delta}^9$-tetrahydrocannabinol (${\Delta}^9$-THC) were evaluated for their potential for psychological dependence. The conditioned place preference test (unbiased method) and self-administration test (fixed ratio of 1) using rodents were conducted. $K_i$ values of the three synthetic cannabinoids were calculated as supplementary data using a receptor binding assay and overexpressed $CB_1$ protein membranes to compare dependence potential with $CB_1$ receptor binding affinity. All mice administered JWH-073, 081, or 210 showed significantly increased time spent at unpreferred space in a dose-dependence manner in the conditioned place preference test. In contrast, all tested substances except ${\Delta}^9$-THC showed aversion phenomenon at high doses in the conditioned place preference test. The order of affinity to the $CB_1$ receptor in the receptor binding assay was JWH-210 > JWH-081 >> JWH-073, which was in agreement with the results from the conditioned place preference test. However, no change in self-administration was observed. These findings suggest the possibility to predict dependence potential of synthetic CBs through a receptor binding assay at the screening level.

• 대중서사연구
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• 제26권3호
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• pp.87-120
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• 2020

이 연구는 익숙한 장르 문법이나 코드 활용이 자유로운 대중문화콘텐츠의 특성이 반영된 웹소설, 특히 로맨스판타지 장르의 '책빙의물'의 특성에 주목하였다. 주인공이 자신이 현실세계에서 읽은 작품 속으로 들어가는 상상력은 웹소설의 기존 장르문법을 재해석한 것으로, 이 모티프를 연구하는 것은 장르 내 상호텍스트성 검토에 유의미하다. 로맨스 판타지 장르에서 책빙의물은 일반 판타지 장르(퓨전판타지, 현대판타지 등)와 차이점을 보이는데, 장르의 젠더적 특성을 드러내는 데도 이 연구의 목적을 들 수 있다. 이 연구는 책빙의물이 기존 서사에 대한 상호텍스트적 해석에서 탄생되었으며, 따라서 차원이동, 회귀, 대체역사물과 친연성을 갖는다고 보았으며, 팬픽션의 글쓰기 규범을 참고했다고 보았다. 이를 통해 앞으로 기존 서사의 재조합과 장르 간 간섭이 지속될 것을 예측할 수 있었다. 다음으로 로맨스판타지 장르에서의 책빙의물은 주변인이 주인공이 되어 서사를 전복함에도 상당히 보수적이고 자기계발적인 논리를 드러낸다고 보았다. 등장인물들은 현실세계의 미래지식을 활용해 파멸이 예언된 생존세계에서 투쟁해야 한다. 윤리적이고 자조(self-help)적인 주체의 등장은 흥미롭지만, 한편으로 로맨스가 인물들의 생존과 성취의 한 방식이라는 점에서 보수성을 살펴볼 수 있었다. 이 연구는 로맨스판타지 장르에서 상당히 빠르게 시작된 책빙의물의 특성을 검토하고 그 등장배경과 특성을 검토했다는 점에서 연구의의를 갖는다. 물리적인 작품 수합에 한계가 있고, 플랫폼이 한정되었다는 한계가 있었다. 위의 한계는 추후 등장하는 작품들을 좀 더 검토하며 보완하고자 한다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.27-27
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• 2011

For the design of real applicable molecular devices, current-voltage properties through molecular nanostructures such as metal-molecule-metal junctions (molecular junctions) have been studied extensively. In thiolate monolayers on the gold electrode, the chemical bonding of sulfur to gold and the van der Waals interactions between the alkyl chains of neighboring molecules are important factors in the formation of well-defined monolayers and in the control of the electron transport rate. Charge transport through the molecular junctions depends significantly on the energy levels of molecules relative to the Fermi levels of the contacts and the electronic structure of the molecule. It is important to understand the interfacial electron transport in accordance with the increased film thickness of alkyl chains that are known as an insulating layer, but are required for molecular device fabrication. Thiol-tethered RuII terpyridine complexes were synthesized for a voltage-driven molecular switch and used to understand the switch-on mechanism of the molecular switches of single metal complexes in the solid-state molecular junction in a vacuum. Electrochemical voltammetry and current-voltage (I-V) characteristics are measured to elucidate electron transport processes in the bistable conducting states of single molecular junctions of a molecular switch, Ru(II) terpyridine complexes. (1) On the basis of the Ru-centered electrochemical reaction data, the electron transport rate increases in the mixed self-assembled monolayer (SAM) of Ru(II) terpyridine complexes, indicating strong electronic coupling between the redox center and the substrate, along the molecules. (2) In a low-conducting state before switch-on, I-V characteristics are fitted to a direct tunneling model, and the estimated tunneling decay constant across the Ru(II) terpyridine complex is found to be smaller than that of alkanethiol. (3) The threshold voltages for the switch-on from low- to high-conducting states are identical, corresponding to the electron affinity of the molecules. (4) A high-conducting state after switch-on remains in the reverse voltage sweep, and a linear relationship of the current to the voltage is obtained. These results reveal electron transport paths via the redox centers of the Ru(II) terpyridine complexes, a molecular switch.

• World Technopolis Review
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• 제8권1호
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• pp.59-70
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• 2019

Where is a better place to live? In the coming era, this should be more than simply a livable place. It should be an adaptable place that has a flexible system adaptable to any new situation in terms of diversity. Customization and real-time operation are needed in order to realize this technologically. We expect a smart city to have a flexible system that applies technologies of self-monitoring and self-response, thereby being a promising city model towards being a better place to live. Energy demand and supply is a crucial issue concerning our expectations for the flexible system of a smart city because it is indispensable to comfortable living, especially city living. Although it may seem that energy diversification, such as the energy mix of a country, is a matter of overriding concern, the central point is the scale of place to build grids for realizing sustainable urban energy systems. A traditional hard energy path supported by huge centralized energy systems based on fossil and nuclear fuels on a national scale has already faced difficult problems, particularly in terms of energy flexibility/resilience. On the other hand, an alternative soft energy path consisting of small diversified energy systems based on renewable energy sources on a local scale has limitations regarding stability, variability, and supply potential despite the relatively light economic/technological burden that must be assumed to realize it. As another alternative, we can adopt a holonic path incorporating an alternative soft energy path with a traditional hard energy path complimentarily based on load management. This has a high affinity with the flexible system of a smart city. At a system level, the purpose of all of the paths mentioned above is not energy itself but the service it provides. If the expected energy service is fixed, the conclusive factor in choosing a more appropriate system is accessibility to the energy service. Accessibility refers to reliability and affordability; the former encompasses the level of energy self-sufficiency, and the latter encompasses the extent of energy saving. From this point of view, it seems that the small diversified energy systems of a soft energy path have a clear advantage over the huge centralized energy systems of a hard energy path. However, some insuperable limitations still remain, so it is reasonable to consider both energy systems continuing to coexist in a multiplexing energy system employing a holonic path to create and maintain reliable and affordable access to energy services that cover households'/enterprises' basic energy needs. If this is embodied in a smart city concept, this is nothing else but smart energy inclusion. In Japan, following the Fukushima nuclear accident in 2011, a trend towards small diversified energy systems of a soft energy path intensified in order to realize a nuclear-free society. As a result, the Government of Japan proclaimed in its Fifth Strategic Energy Plan that renewable energy must be the main source of power in Japan by 2050. Accordingly, Sony vowed that all the energy it uses would come from renewable sources by 2040. In this situation, it is expected that smart energy inclusion will be achieved by the Japanese version of a smart grid based on the concept of a minimum cost scheme and demand response.

• International Journal of Oral Biology
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• 제37권4호
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• pp.181-188
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• 2012

As the demand for large-scale analysis of gene expression using DNA arrays increases, the importance of the surface characterization of DNA arrays has emerged. We compared the efficiency of molecular biological applications on solid-phases with different surface polarities to identify the most optimal conditions. We employed thiol-gold reactions for DNA immobilization on solid surfaces. The surface polarity was controlled by creating a self-assembled monolayer (SAM) of mercaptohexanol or hepthanethiol, which create hydrophilic or hydrophobic surface properties, respectively. A hydrophilic environment was found to be much more favorable to solid-phase molecular biological manipulations. A SAM of mercaptoethanol had the highest affinity to DNA molecules in our experimetns and it showed greater efficiency in terms of DNA hybridization and polymerization. The optimal DNA concentration for immobilization was found to be 0.5 ${\mu}M$. The optimal reaction time for both thiolated DNA and matrix molecules was 10 min and for the polymerase reaction time was 150 min. Under these optimized conditions, molecular biology techniques including DNA hybridization, ligation, polymerization, PCR and multiplex PCR were shown to be feasible in solid-state conditions. We demonstrated from our present analysis the importance of surface polarity in solid-phase molecular biological applications. A hydrophilic SAM generated a far more favorable environment than hydrophobic SAM for solid-state molecular techniques. Our findings suggest that the conditions and methods identified here could be used for DNA-DNA hybridization applications such as DNA chips and for the further development of solid-phase genetic engineering applications that involve DNA-enzyme interactions.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.417-421
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• 2004

A normal prion protein (PrPc) is converted to a protease resistant isoform (PrPsc) by an apparent self-propagating activity in bovine spongiform encephalopathies (BSE), which is a neurodegenerative disease. The cDNA encoding bovine PrP open reading frame (ORP) in Korean cattle was cloned by polymerase chain reaction (PCR). The cloned cDNA had a length of 795 base pairs which coded for a protein of 264 amino acid residues with a calculated molecular mass of 28.6 kDa. Identities of 90, 90, 79 and 78% on nucleotide and 94, 94, 84, and 84% on amino acid sequence were shown to PrP genes from sheep, goat, human, and mouse, respectively. The cloned DNA was ligated into the pQE30 expression vector and transformed into E. coli M15. The PrP was expressed by induction with isopropyl-$\beta$-D-thiogalactoside (IPTG) and purified on the Ni-NTA affinity column. High specific activities of the recombinant PrP were observed in the fraction of pH 5.8 eluate and showed a molecular mass of-29 kDa on SDS-PAGE and Western blot analysis.

• 한국주거학회논문집
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• 제19권6호
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• pp.145-155
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• 2008

This study examined the actual use and users‘ satisfaction with the university dormitories in Gwangju and Chonnam and analyse the related variables that affect on them. Self-administered questionnaires were provided to 200 university students from Gwangju and the Chonnam area. The collected data was used to conduct comparative and descriptive statistical analysis: T-test Below are the study findings. Female students express more satisfaction with community affinity than male students in the satisfaction about socio-psychological environment. Students residing in the rooms for 2 persons express more satisfaction with the division of private space with common space, study appropriateness etc. than those residing in the rooms for 4 persons. Students residing in the cluster-type rooms express more satisfaction with the division of private space with common spaces study appropriateness and relationship with other students in other rooms etc. In relation to the room environment, female students express less dissatisfaction with the noise than male students. Students residing in the rooms for 2 persons express more satisfaction with the room size, interior temperature pleasantness, brightness etc. than those residing the in rooms for 4 persons. Students residing in the cluster-type rooms express more satisfaction with the bed size, the condition of interior finishing, interior temperature pleasantness, brightness and interior air pleasantness etc. than those residing in unit-rooms. In relation to common using environment, male students express more satisfaction with the library than female students. And students residing in the rooms for 4 persons and those residing in the cluster-type rooms express more satisfaction with lounge than those residing in the rooms for 2 persons and those residing in the unit-rooms. Organizing the university domitory planning according to the students' demands, based on this study, the change to general function of domitory in sleeping, studying, leisure activity and culture acitivity is needed. Interior environment technical factor of interior air pleasantness and noise should be improved.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2013년도 춘계학술대회
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• pp.270-272
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• 2013

해안시설물을 주로 파랑과 흐름의 작용으로부터 보호하기 위하여 세굴 방호시설물의 구성요소인 자체연결의 바이오 블록을 디자인하였다. 이 블록은 해저경사면, 해빈, 해안저면에 적응하여 파랑의 충격 및 침식작용에 대응하도록 하였다. 수면상 및 해저에 설치시 일련의 수리실험을 통해 파의 반사를 조사할 필요가 있었으며 입사파에 대한 반사성능 및 파력의 비교도 필요하다. 본 연구에서는 새로 설계한 바이오 블록의 설치로 반사계수를 줄임으로써 해안경사 및 해저면을 세굴로부터 보호하고, 설치한 블록의 안정성을 확인하기 위하여 수리모델실험을 수행하였다. 또한, 블록의 각 요소를 개량한 디자인에 대해 현장여건을 고려하여 시험하였다. 실험의 결과로부터 개발한 블록의 현장적용성과 거치방안이 후속으로 논의될 것이다.

• 센서학회지
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• 제19권4호
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• pp.306-312
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• 2010

We have detected deoxynivalenol(DON) using a metal-oxide-semiconductor field-effect-transistor(MOSFET)-based biosensor. The MOSFET-based biosensor is fabricated by a standard complementary metal-oxide-semiconductor(CMOS) process, and the biosensor's electrical characteristics were investigated. The output of the sensor was stabilized by employing a reference electrode that applies a fixed bias to the gate. Au which has a chemical affinity for thiol was used as the gate metal to immobilize a self-assembled monolayer(SAM) made of 16-mercaptohexadecanoic acid(MHDA). The SAM was used to immobilize anti-deoxynivalenol antibody. The carboxyl group of the SAM was bound to the anti- deoxynivalenol antibody. Anti-deoxynivalenol antibody and deoxynivalenol were bound by an antigen-antibody reaction. In this study, it is confirmed that the MOSFET-based biosensor can detect deoxynivalenol at concentrations as low as 0.1 ${\mu}g$/ml. The measurements were performed in phosphate buffered saline(PBS; pH 7.4) solution. To verify the interaction among the SAM, antibody, and antigen, surface plasmon resonance(SPR) measurements were performed.

• 시큐리티연구
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• 제33호
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• pp.51-80
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• 2012

이 연구의 목적은 특정직 경호공무원의 전직 역량에 대한 특정직 경호공무원들의 보유수준 조사를 통한 전직지원방안을 제시하는데 있으며, 이를 위해 특정직 경호공무원 중 전직관련 대상자로서 40대 이상 5급 사무관 이상 전직 유경험자인 전직 성공자와 전직 실패자, 전직 희망자인 전직 예정자 600명을 대상으로 지식역량군의 전문지식, 전직관련 지식, 자기이해, 조직이해, 기술역량군의 직무기술역량, 직무수행기술, 문제해결기술, 혁신기술, 커뮤니케이션기술, 조직관리기술, 위기관리기술, 경력개발기술, 인적네트워크 활용기술, 태도 및 감정역량군의 긍정적 태도, 적극적 태도, 책임감, 직업정신, 헌신적 태도, 친화력, 자기조절능력, 가치 및 윤리역량군의 윤리의식, 도덕성 등 4개 역량군 22개 하위역량의 보유수준에 대한 설문조사를 실시하였다. 최종적으로 유효한 설문 응답자 153명의 4개 역량 군 22개 하위역량 항목에 대한 설문조사 분석결과, 현재 보유수준 값이 4.0이상인 전문지식, 긍정적 태도, 책임감, 윤리의식, 도덕성 역량 등에 있어서는 어느 정도 갖추었다고 생각하고 있으나, 그 외 역량에 대해서는 보통이하 점수로 다소 낮게 나타나, 이들 역량들에 대해서는 아직은 부족하다고 인식하고 있다. 따라서 이러한 역량 강화를 위한 특정직 경호공무원의 성공적인 전직지원방안으로서는 전직에 대한 인식과 개념을 재정립하고 현실을 직시할 수 있도록 눈높이를 낮추어야 하며, 전직에 필요한 역량으로서 다소 부족하다고 느끼고 있는 역량들에 대해서는 평소에 관심을 가지고 적극적인 개발 및 강화 노력이 요구된다. 제도적 차원에서 이러한 전직 역량을 갖출 수 있도록 퇴직 전 중 후 전직 교육훈련 강화 및 현 교육훈련체계 반영, 수요자 중심의 온-오프 상 교육훈련시스템 운영, 학습조직화 등 전직교육훈련 인프라를 구축할 필요성이 있다.