• Bulletin of the Korean Chemical Society
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• 제16권9호
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• pp.870-873
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• 1995

A new ternary transition-metal selenide, Ta2Ni3Se8 has been synthesized from a eutectic halide flux. The structure of this phase has been characterized by single crystal X-ray diffraction techniques. The compound crystallizes in the orthorhombic system (D2h9-Pbam, a= 14.788(4) Å, b= 10.467(3) Å, c=3.4563(8) Å) with two formula units in the unit cell. This compound adopts the Nb2Pd3Se8 structure type. Hence, there are two chains of edge-sharing selenium trigonal prisms centered by tantalum atoms and these chains are interconnected through two kinds of nickel atoms. Nickel occupies both square planar and square pyramidal sites as does palladium in Nb2Pd3Se8. Electrical conductivity measurements indicate that this material is semiconducting.

• Toxicological Research
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• 제30권4호
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• pp.267-276
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• 2014

Exposures to lead (Pb) are associated with neurological problems including psychiatric disorders and impaired learning and memory. Pb can be absorbed by iron transporters, which are up-regulated in hereditary hemochromatosis, an iron overload disorder in which increased iron deposition in various parenchymal organs promote metal-induced oxidative damage. While dysfunction in HFE (High Fe) gene is the major cause of hemochromatosis, the transport and toxicity of Pb in Hfe-related hemochromatosis are largely unknown. To elucidate the relationship between HFE gene dysfunction and Pb absorption, H67D knock-in Hfe-mutant and wild-type mice were given drinking water containing Pb 1.6 mg/ml ad libitum for 6 weeks and examined for behavioral phenotypes using the nestlet-shredding and marble-burying tests. Latency to nestlet-shredding in Pb-treated wild-type mice was prolonged compared with non-exposed wild-types (p < 0.001), whereas Pb exposure did not alter shredding latency in Hfe-mutant mice. In the marble-burying test, Hfe-mutant mice showed an increased number of marbles buried compared with wild-type mice (p = 0.002), indicating more repetitive behavior upon Hfe mutation. Importantly, Pb-exposed wild-type mice buried more marbles than non-exposed wild-types, whereas the number of marbles buried by Hfe-mutant mice did not change whether or not exposed to Pb. These results suggest that Hfe mutation could normalize Pb-induced behavioral alteration. To explore the mechanism of repetitive behavior caused by Pb, western blot analysis was conducted for proteins involved in brain dopamine metabolism. The levels of tyrosine hydroxylase and dopamine transporter increased upon Pb exposure in both genotypes, whereas Hfe-mutant mice displayed down-regulation of the dopamine transporter and dopamine D1 receptor with D2 receptor elevated. Taken together, our data support the idea that both Pb exposure and Hfe mutation increase repetitive behavior in mice and further suggest that these behavioral changes could be associated with altered dopaminergic neurotransmission, providing a therapeutic basis for psychiatric disorders caused by Pb toxicity.

• 생명과학회지
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• 제18권1호
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• pp.1-8
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• 2008

이 연구는 지구성 운동과 셀레니움 투여가 Coto-Kakizaki 쥐의 젖산수송 능력에 독립적으로 혹은 상호작용하여 영향을 미치는가를 구명하는데 그 목적이 있다. 실험동물들의 집단은 비교집단(n=10, SED), 지구성 운동집단(n=10, EXER), 셀레니움 투여집단(n=10, SELE)과 지구성 운동과 셀레니움 투여 병행 집단(n=10, COMBI)으로 분류하여 6주간 실험을 실시하였다. 6주간 실험 처치 후 체중은 비교집단에 비해 지구성 운동집단, 셀레니움 투여집단과 지구성 운동과 셀레니움 투여 병행 집단이 현저하게 감소하였으며 당 부하검사를 실시한 결과에서도 비교집단에 비해 지구성 운동집단, 셀레니움 투여집단과 지구성 운동과 셀레니움 투여 병행 집단이 90분과 120분 사이에서 혈당 수준이 유의한 낮은 것으로 나타났다. 간 글리코겐 수준은 비교집단과 셀레니움 투여집단에 비해 지구성 운동집단과 지구성 운동과 셀레니움 투여 병행 집단이 유의하게 높은 것으로 나타났다. 가자미근과 족저근의 글리코겐 수준도 비교집단과 셀레니움 투여집단에 비해 지구성 운동집단과 지구성 운동과 셀레니움 투여 병행 집단이 현저하게 높은 것으로 나타났다. 혈액 생화학 성분의 경우, 지구성 운동집단, 셀레니움 투여집단과 지구성 운동과 셀레니움 투여 병행 집단이 비교집단에 비해 고밀도 지단백 수준 증가와 함께 혈장 젖산, 혈청 중성지방, 인슐린, 총 콜레스테롤과 HOMA-IR 수준이 현저하게 감소한 것으로 나타났다. 특히 혈청 중성지방 수준은 지구성 운동집단과 지구성 운동과 셀레니움 투여 병행 집단이 셀레니움 투여집단에 비해 유의하게 낮은 것으로 나타났다. 이 연구에 가장 중요한 결과는 혈당과 젖산 수송과 관련된 단백질 발현 수준이 6주간의 실험처치 후에 지구성 운동집단, 셀레니움 투여집단과 지구성 운동과 셀레니움 투여 병행 집단이 비교집단에 비해 가자미근의 MCT1과 미토콘드리아 MCT1 단백질 발현수준이 현저하게 증가하였다는 결과와 함께 가자미근과 족저근의 MCT4 단백질 발현 수준도 지구성 운동집단, 셀레니움 투여집단과 지구성 운동과 셀레니움 투여 병행 집단이 비교집단에 비해 현저하게 증가하였다는 결과이다. 이러한 결과를 근거로 볼 때 지구성 운동과 셀레니움 투여는 독립적으로 혹은 상호작용하여 혈당과 젖산수송 능력을 개선시키는데 도움이 된다는 것을 알 수 있으며 특히 인슐린 저항 특성과 함께 고젖산혈증을 나타내는 제 II형 당뇨 환자들의 당뇨 처치를 위한 방법으로 활용할 만한 가치가 있는 것으로 생각된다.

• Nutrition Research and Practice
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• 제4권3호
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• pp.215-221
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• 2010

This study was performed to analyze the relationship among sebum hydration content of the skin and nutritional intake, serum antioxidant minerals and antioxidant enzymes, and lipid peroxide concentration in 50 female subjects in their 20s. The skin type was divided into Dry Skin, Mixed Skin, and Oily Skin, and the dry skin group was 14%, the mixed skin group was 56%, and the oily skin group was 30% of all subjects. The average age of the subjects was $20.54{\pm}1.43$ years and BMI was 20.66. The average sebum content in each group was in the order of T-zone>forehead>chin>cheek. In case of the T-zone, a significant difference between the dry skin group and the oily skin group was observed, suggesting that the area is most sensitive to sebum content by skin type. Significant differences were not observed in energy and nutrient intakes by skin type. Serum concentrations of antioxidant minerals such as copper, manganese, zinc and selenium were not significantly different among the groups, but the dry skin group tended to be higher than the oily skin group. Serum catalase was significantly higher in the oily skin group (P < 0.05), and MDA was significantly higher in the mixed skin group (P < 0.05). The hydration of the cheek and serum zinc showed a negative correlation, and the sebum content of the cheek and GPx showed a significant negative correlation. The hydration of the forehead and serum copper showed a significant negative correlation, and the hydration of the forehead and GPx showed a significant positive correlation. The hydration of the chin and serum SOD showed a significant positive correlation. With these results, it is considered that the basic condition of nutritional status can affect the skin health.

• 대한전기학회논문지
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• 제43권2호
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• pp.296-302
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• 1994

Polycrystalline CuInSeS12T thin were prepared by depositing Cu/In layer, which was sequentially sputtered varying the Cu/(Cu+In) mole ratio, on glass substrate and selenizing with selenium metal vapor in a nitrogen atmosphere. Compositional and structural, characterization was carried out by X-ray diffraction (XRD), wavelength-dispersive spectroscopy(WDS), and scanning electron microscope(SEM). Electrical characterization was carried out by the measurements of Hall effect, electrical resistivity. Large indium loss occurs in early stage of the selenization process. The selenized films which had mole ratios larger than 0.28 have chalcopyrite CuInSeS12T phase and these that had less mole ratios have sphalerite phase. The selenized films containing CuS1xTSe phase have Cu-rich CuInSeS12T phase and these that did not contain CuS1xTSe have In-rich CuInSeS12T phase. By optimizing the sputtering conditions,it is possible to fabricate CuInSeS12T thin films which have little secondary phases and an appropriate hole concentration (10S015T ~ 10S016TcmS0-3T) for solar cells.

• Journal of Electrochemical Science and Technology
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• 제4권4호
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• pp.140-145
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• 2013

This study describes the electrodeposition of $Cu_2Se$ thin films with a two-step approach that is based on the initial modification of polycrystalline Au electrode with a selenium overlayer followed by a cathodic stripping of the layer as $Se^{2-}$ in a 1 M lactic acid electrolyte containing $Cu^{2+}$ ions. For this two-step approach to be effective, the $Cu^{2+}$ reduction potential should be shifted to more negative potentials passed potentials for the reduction of Se to $Se^{2-}$. This was accomplished by the complexation of $Cu^{2+}$ ions with lactic acid. The resultant $Cu_2Se$ films were characterized by linear sweep voltammetry combined with electrochemical quartz crystal microgravimetry, UV-vis absorption spectrometry and Raman spectroscopy. Photoelectrochemical experiments revealed that $Cu_2Se$ synthesized thus, behaved as a p-type semiconductor.

• 한국수산과학회지
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• 제50권6호
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• pp.707-713
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• 2017

Saccharina japonica is a type of seaweed rich in dietary fiber, iodine, calcium, and selenium. We investigated the quality characteristics and antioxidant activities of gruel made with S. japonica powder, and identified the best ratio of powder to gruel to maximize its health effects. The gruel was prepared with rice, glutinous rice, GABA (${\gamma}-aminobutyric$ acid)-enriched sea tangle fermented in lactic acid (0.3%), and various amounts (0-10% gruel volume) of S. japonica powder. The pH, color, viscosity, spreadability, antioxidant activities, and sensory evaluation of the gruel were investigated. Spreadability and pH decreased, whereas viscosity and antioxidant activities increased, with increasing powder content. In addition, color lightness decreased significantly, whereas redness and yellowness increased. In sensory tests, color, aroma, and texture were rated highest for gruel with 5% powder. On the other hand, flavor and overall acceptability were considered optimal in gruel with 7% powder.

• Korean Chemical Engineering Research
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• 제57권6호
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• pp.781-789
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• 2019

연구실 실험, 파일럿 플랜트 및 반응기 운전 중 화학물질에 의한 안전사고가 발생하고 있다. 합성 실험을 시작하기전 사고예방을 위해 관련 정보들을 찾아볼 필요가 있으며, 공정설계 단계에서도 반응 폭주 예방을 위한 반응정보의 확보는 필수적이다. 합성반응 관련 정보는 인터넷을 포함해 다양한 source가 존재하지만, 검색에 오랜 시간이 걸리고, 합성법마다 사용되는 물질도 달라 적정경로 선택의 어려움이 있다. 연구자들의 합성경로 검색시간단축과 합성 시 존재할 수 있는 위험성 및 중간생성물질들의 확인에 도움을 주고자 본 연구는 스마트 합성경로 탐색시스템을 제안하였다. 제안한 탐색시스템은 Python 패키지인 Selenium을 사용한 Web scraping 및 Web crawling을 통해 인터넷에 존재하는 정보를 수집하여 DB를 자동으로 갱신한다. 경로 탐색 알고리즘은 depth-first search에 기반하여 목표 물질을 기준으로 탐색을 진행하고, 유해화학물질 등급, 수율 등을 구분하여, 제한된 경로 단계 수치내에 있는 모든 합성 경로를 제안한다. 또한 각자의 연구 목적에 맞게 연구원들이 가진 비공개 데이터를 형식을 맞춰 DB에 등록하여 확장할 수 있다. 시스템은 차후에 무료 사용이 가능하도록 open source로 공개할 예정이다. 개발 시스템은 연구자들이 제안된 경로를 참고하여 더 안전한 반응 방법을 찾고, 사고의 예방에도 도움을 줄 것으로 기대된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.319-324
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• 2006

Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology industry. For this reason, many research groups, including our laboratory, have been trying to develop serum-free media (SFM) or serum-supplemented media (SSM) for special or multi-purpose cell lines. The Chinese hamster ovary (CHO) cell, for example, is frequently used to produce proteins and is especially valuable in the large-scale production of pharmaceutically important proteins, yet information about its genome is lacking. Also, SFMs have only been evaluated by comparing growth patterns for cells grown in SFMs with those grown in SSM or by measuring the titer of the target protein obtained from cells grown in each type of medium. These are not reliable methods of obtaining the type of information needed to determine whether an SFM should be replaced with an SSM. We carried out a cDNA microarray analysis to evaluate MED-3, an SFM developed in our laboratory, as a CHO culture medium When CHO cells were cultured in MED-3 instead of an SSM, several genes associated with cell growth were down-regulated, although this change diminished over time. We found that the insulin-like growth factor (IGF) gene was representative of the proteins that were down-regulated in cells cultured in MED-3. When several key supplements - including insulin, transferrin, ethanolamine, and selenium - were removed from MED-3, the IGF expression was consistently down- regulated and cell growth decreased proportionately. Based on these results, we concluded that when an SFM is used as a culture medium, it is important to supplement it with substances that can help the cells maintain a high level of IGF expression. The data presented in this study, therefore, might provide useful information for the design and development of SFM or SSM, as well as for the design of genome-based studies of CHO cells to determine how they can be used optimally for protein production in pharmaceutical and biomedical research.

• 한국토양비료학회지
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• 제41권6호
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• pp.369-373
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• 2008

Mg-Al LDH를 이용하여 수용액중 비소와의 반응특성을 규명하고 비소 제거제로서 활용가능성을 규명하기 위하여 비소의 흡착특성, 제거효율 및 제거기작에 대한 조사를 하였다. Mg-Al LDH는 소성(calcination)에 의한 탈수로 Mg oxide 형태를 나타내었고 비소를 흡착시킨 결과 반응 22시간 이후에 흡착평형에 도달하였으며 흡착량은 약 530 mmol/kg정도였다. 반응농도별 LDH의 등온흡착은 L-type의 흡착반응을 나타내었다. 소성된 Mg-Al LDH는 용액중에서 재수화(rehydration) 될 때 비소가 LDH의 구조의 복구과정(reconstruction)에서 이온교환 반응에 의해 층간삽입이 일어나는 것으로 나타났다. LDH에 대한 arsenate와 phosphate, arsenate와 sulfate의 경쟁흡착 결과 arsenate와 phosphate의 선택성은 비슷한 편이었고, arsenate는 sulfate에 비해 선택성이 우수하였다. 따라서 calcined Mg-Al LDH는 비교적 높은 비소 제거효율을 나타내므로 비소 제거제로서 사용 가능성이 매우 높은 것으로 판단된다.