• KSBB Journal
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• 제24권5호
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• pp.463-468
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• 2009

셀레늄 함유 펩타이드 (셀레늄 펩타이드)는 무기 셀레늄이 포함된 배지에서 효모를 배양하여 효모의 자가분해에 의해 만들었다. 효모 배양에 의해 만들어진 셀레늄 펩타이드는 GPx 유사활성을 보였으며, UVB 조사가 된 인간 섬유아세포에 대하여 세포 보호효과를 나타냈다. 셀레늄 나이트레이트는 $10^{-9}$ 몰 농도에서 낮은 세포독성을 보인반면 셀레늄 펩타이드는 최소의 독성만을 보였다. 또한 셀레늄 펩타이드는 인간 섬유아세포의 성장과 procollagen type I을 증가시킨 반면 MMP-1의 감소를 가져왔다. 연구결과 셀레늄 펩타이드가 무독성의 항산화제로서의 가능성을 보여주었다.

• Journal of Animal Science and Technology
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• 제59권4호
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• pp.7.1-7.13
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• 2017

Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

• Journal of Gastric Cancer
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• 제12권4호
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• pp.217-222
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• 2012

Purpose: We conducted this study to study the clinical correlation between the characteristics of gastric cancer and serum selenium and zinc levels. Materials and Methods: The following data were measured in the baseline serum selenium and zinc levels of 74 patients with curative gastrectomy subsequent to confirmed gastric cancer, from March 2005 to August 2012. Results: Among the 74 gastric cancer patients, 53 patients were male. Mean serum selenium and zinc levels were $118.7{\pm}33.1$ ug/L and $72.2{\pm}24.3$ ug/dl, respectively. Seven patients (9.5%) showed lower selenium level, and 33 patients (44.6%) showed lower zinc level. Serum Selenium level was $99.1{\pm}31.8$ ug/L in cardia cancer group (10 cases) and $121.8{\pm}32.4$ ug/L in non-cardia cancer group (64 cases)(P=0.044). According to tumor gross type, zinc level was $78.7{\pm}29.6$ ug/dl in early gastric cancer (33) and $66.9{\pm}17.8$ ug/dl in advanced gastric cancer (41) (P=0.064). Conclusions: The serum selenium level was highly correlated with the location of gastric cancer. The serum zinc level was lower in advanced gastric cancer.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 하계학술대회 논문집 Vol.3 No.2
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• pp.849-852
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• 2002

Recently, amorphous selenium is used as x-ray conversion material for flat-panel x-ray detector. In this paper, we investigated the effect of breakdown under high voltage and leakage current in PN-type multi-layer structure based on p-type a-Se and n-type conductive thin film. Experimental results show that the multi-layer based detector reduced leakage current because n-type CeO2 conductive layer prevent from hole injection into a-Se layer from collection electrode, Also, the breakdown voltage was improved by dielectric layer between a-Se and top electrode.

• Clinical and Experimental Reproductive Medicine
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• 제45권1호
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• pp.10-16
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• 2018

Objective: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. Methods: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed. The expression of antioxidant enzymes was assessed by reverse-transcription polymerase chain reaction. In addition, the effects of selenium treatment on mitochondrial activity were evaluated in terms of adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species levels. Results: Selenium showed positive effects on the viability and migration activity of trophoblast cells when exposed to hypoxia. Interestingly, the increased heme oxygenase 1 expression under hypoxic conditions was decreased by selenium treatment, whereas superoxide dismutase expression was increased in trophoblast cells by selenium treatment for 72 hours, regardless of hypoxia. Selenium-treated trophoblast cells showed increased mitochondrial membrane potential and decreased reactive oxygen species levels under hypoxic conditions for 72 hours. Conclusion: These results will be used as basic data for understanding the mechanism of how trophoblast cells respond to oxidative stress and how selenium promotes the upregulation of related genes and improves the survival rate and invasive ability of trophoblasts through regulating mitochondrial activity. These results suggest that selenium may be used in reproductive medicine for purposes including infertility treatment.

• Transactions on Electrical and Electronic Materials
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• 제5권4호
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• pp.133-137
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• 2004

The development of digital x-ray detector has been extensively progressed for the application of various medical modalities. In this study, we introduce a new hybrid-type x-ray detector to improve problems of a conventional direct or indirect digital x-ray image technology, which composed of multi-layer structure using a CaWO$_4$ phosphor and amorphous selenium (a-Se) photoconductor. The leakage current of our detector was found to be ∼180 pA/cm$^2$ at 10 V/m, which was significantly reduced than that of a single a-Se detector. The x-ray sensitivity was measured as the value of 4230 pC/cm$^2$/mR at 10 V/m. We found that the parylene thin film between a CaWO$_4$ phosphor and an a-Se layer acts as an insulator to prevent charge injection from indium thin oxide (ITO) electrode into an a-Se layer under applied bias.

• Journal of Nutrition and Health
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• 제51권2호
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• pp.121-131
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• 2018

본 연구는 여자 대학선수들의 혈청 셀레늄과 아연 수준을 일반 여대생과 비교 평가하고, 열량영양소 및 항산화영양소 섭취와의 관계를 평가하고자 하였다. 본 연구는 체육대학의 여자 에어로빅 및 유도선수와 운동을 규칙적으로 하지 않는 일반 여대생을 대상으로 식사섭취 조사와 혈청의 두 미량 무기질의 농도를 측정하였다. 에너지 섭취량은 에어로빅과 유도선수가 각각 2,151.0 kcal와 2,347.7 kcal를 나타내어 일반 여대생에 비해 유의적으로 높았다 그러나 체중 1 kg당 하루 에너지 섭취량은 선수와 일반 여대생간에 유의적 차이가 없었지만 유도선수의 경우 35.8 kcal에 불과하였다. 지질 섭취량은 일반 여대생에 비해 두 선수군에서 유의적으로 높은 반면에, 단백질과 탄수화물의 섭취량은 선수와 일반 여대생 사이에 유의적 차이는 없었다. 지질 중 포화지방산 섭취는 유도선수가 일반 여대생에 비해 유의적으로 높은 섭취를 보였다. 비타민 A, E와 비타민 C의 섭취량은 에어로빅 선수가 일반 여대생에 비해 유의적으로 높으며 INQ도 1이상을 보였다. 그러나 유도선수의 경우 비타민 A와 C의 INQ가 1이하를 나타내어 비타민 A와 C의 영양적 질이 부적절함을 보였다. 철의 섭취량은 에어로빅 선수가 일반 여대생에 비해 유의적으로 높았으나, 셀레늄과 아연 섭취는 유의적 차이가 없었다. 그러나 철과 아연의 INQ는 유도선수에서 유의적으로 낮았다. 에어로빅과 유도선수의 셀레늄의 평균 섭취량은 각각 $106.2{\mu}g$과 $101.9{\mu}g$를 나타내었고, 아연 섭취량은 각각 10.7 mg과 9.3 mg으로 일반 여대생과 비교하여 유의적 차이는 보이지 않았다. 혈청 셀레늄의 평균 농도는 유도선수가 $10.7{\mu}g/dl$을 보여 에어로빅 선수에 비해 유의적으로 낮았지만 이 들 중 셀레늄 결핍자는 없었다. 에어로빅과 유도선수의 평균 혈청 아연농도는 각각 $96.1{\mu}g/dl$과 $90.2{\mu}g/dl$으로서 일반 여대생과 유사하였지만, 에어로빅에서는 아연 결핍이 없으나 유도선수에서 아연 결핍율은 각각 14.3%를 보였다. 혈청 셀레늄농도의 변이는 나이 포화지방산, 비타민 E와 셀레늄 섭취에 의해 35.5%를 설명할 수 있으며, 나이를 제외한 식사요인에 의해서 33.3%를 설명할 수 있었다. 혈청 아연농도의 변이는 포화지방산 섭취에 의해 14.7%를 설명함으로써, 특히 포화지방산 섭취가 두 미량 무기질의 혈청 수준에 부정적인 영향을 줌을 보였다. 결론적으로 여자 대학선수들의 인체 셀레늄 상태는 양호하나 일부 선수에서 아연 영양부족이 우려되며 두 미량 무기질 수준을 유지하기 위해 포화지방산의 섭취를 줄이는 영양개선이 필요함으로 보였다. 나아가 셀레늄의 식사섭취와 인체 수준과의 관계를 명확하게 평가하기 위해서는 식사를 통한 셀레늄 섭취의 정확히 평가가 필요하며, 이에는 식품의 셀레늄 데이터베이스의 개선이 필요한 것으로 사료되며, 아연 영양상태 관련인자를 평가하기 위해서는 아연의 생체 이용률을 고려하는 자료가 필요할 것이다.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.36-39
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• 2003

Many defined cell culture media were formulated over 3() years ago and may be deficient in certain micronutrients whose essentiality has only subsequently been recognised. The objective of this study was to evaluate whether alpha-minimal essential medium (MEM) supplemented with 10% foetal bovine serum contained sufficient selenium for optimal activity of the selenium containing enzymes cytosolic glutathione peroxidase (cGPx) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) in cultured Chinese hamster ovary (CHO) cells. Additionally, the effect of zinc deficiency and metallothionein (MT) overexpression on cGPx and PHGPx activity was studied. The addition of 100 nM of selenous acid to the culture medium increased cGPx expression by 10-fold and PHGPx by about 2-fold in both wild-type CHO-K1 cells and CHO-K1 cells overexpressing mouse MT-1. Zinc deficiency had no significant effect on enzyme activity, but cells overexpressing mouse MT-1 had higher levels of cGPx activity. Zinc deficiency decreased cell survival but overexpression of MT-1 was partially protective, probably because its presence in quantity favoured the uptake, sequestration and cellular retention of any remaining zinc. This study demonstrates that selenium in complete alpha-MEM is insufficient for optimal cGPx and PHGPx activity and may compromise the cellular response to oxidative stress.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.305-316
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• 2005

This study was conducted to determine the total amount and type of seleniwn (Se) in the Se-enriched mushroom and its spent mushroom composts (SMC), and to investigate the metabolism in relation to Se accwnulation in the mushroom. Mushrooms, Flammulina velutipes, used in this study were grown for 60 days by adding 2 rng of inorganic Se (Na2Se03) per kg of mushroom composts (MC) on as-fed basis and were compared with normal mushrooms grown on the non Se-supplemented Me. Total Se contents for Se-treated mushrooms were significantly increased (P < 0.0001) by 20-fold (4.51 $\mu$/ g of dry) compared to Se-untreated (0.23 $\mu$/ g of dry). On the contrary, organic Se ratio was significantly lower (P < 0.0001) in the Se-treated mushroom (72.3 %) than the Se-untreated one (100 %, not analytically detected of inorganic Se). Se distribution upon a length in the Se-treated mushrooms was the highest in the bottom part (6.86 $\mu$/ g of dry) near to MC, and top and middle parts were significantly lower (3.71 and 3.01 $\mu$/ g of dry, respectively; P < 0.001) than the bottom. In the SMC from Se-treated mushrooms, the significant amount of Se (5.04l1g/g of dry) was remained, but that from the Se-untreated mushrooms was significantly low (P$\mu$ / g of dry. Se-treated SMC showed a high ratio of organic Se (65.67 %), suggesting that the significant amount of inorganic Se in the SMC was converted to organic Se by mushroom mycelia. Prior to mycelia inoculation in the mushroom culture, the sterilization of MC brought approximately 18% of Se loss in the MC. Apparent and net accumulation rates (%) for Se into mushrooms were 14.81 and 10.14 %, respectively, resulting from the Se volatilization into the air via metabolic process of mushroom itself. The result of this study shows that inorganic Se addition to MC for mushroom improved the organic Se contents in the mushroom and SMC. This study showed the possibility that Se in Se-enriched mushroom and SMC could be utilized as Se sources of food for human as well as feed for livestock.

• 한국초지조사료학회지
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• 제33권4호
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• pp.281-289
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• 2013

본 연구는 다양한 수준의 셀레늄 강화 청보리 사일리지와 무기셀레늄을 급여 시 비육기 거세한우의 생산성, 혈액 성상, 도체특성 및 조직 내 셀레늄 축적에 미치는 영향을 조사하였다. 시험은 거세비육우 20두를 공시하였고, 처리구는 셀레늄 수준에 따라 0.1 ppm Se(대조구), 0.3 ppm Se (T1), 0.9 ppm Se (T2) 및 무기셀레늄 0.9 ppm Se (T3) 급여구로 4처리를 나누어 처리구 구당 5두씩 완전임의 배치하여 90일간 사양시험을 실시하였다. 각 시험사료는 셀레늄 강화 및 셀레늄 무처리 관행 청보리 사일리지를 조합하여 건물기준 30% 함유하도록 배합하였다. 실험사료 내에 셀레늄 강화수준은 사료섭취량과 증체에 영향을 미치지 않았고, 혈중 총 지질과 중성지방농도는 대조구보다 T2구가 유의하게 낮았다(p<0.05). 혈중 면역글로불린은 셀레늄함유량이 많을수록 높게 나타나 T2와 T3구가 대조구보다 유의하게 높았다(p<0.05). 혈중 셀레늄농도는 유기태 셀레늄을 0.9 ppm 급여한 T2구가 가장 높았다(p<0.05). 도체율, 배최장근, 등지방두께, 근내지방도, 육색 및 지방색은 셀레늄급여로 인한 효과가 나타나지 않았다. 또한, 사료 내 셀레늄 수준 및 형태는 육질 및 육량등급 간에 관련성이 형성되지 않았다. 신장, 간장 및 후지 내 셀레늄 함량은 사료 내 셀레늄 강화수준이 증가함에 따라 유의하게 직선적으로 증가하였고(p<0.05), 무기셀레늄급여는 대조구와 유의한 차이가 나타나지 않아, 조직 내 셀레늄함량 증가에 도움이 되지 않았다(p>0.05). 이상의 결과에서 셀레늄 강화 청보리 사일리지 내 존재하는 셀레늄은 조직 내 셀레늄함량을 증가시키기에 유효한 셀레늄급원인 것으로 나타났고, 셀레늄 강화 청보리 최고수준(0.9 ppm Se)으로 급여하여 생산된 한우 후지육 100 g에 함유되어 있는 셀레늄은 성인기준 1일 최소 셀레늄 권장량($40{\mu}g$)의 약 69%를 충족시키는 것으로 조사되었다.