• Journal of Animal Science and Technology
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• 제48권6호
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• pp.897-906
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• 2006

본 연구는 셀레늄강화 한우고기 생산을 위하여 셀레늄강화 버섯폐배지(Se-SMC)가 함유된 셀레늄사료(사료내 셀레늄함량: 0.9ppm)를 활용하여 급여사양기간(2개월, 3개월, 4개월)을 달리 하였을 때, 비육후기 거세한우의 조직 내 셀레늄 함량과 혈중 글루타치온 과산화효소(GSH-Px) 활성에 미치는 영향을 조사하여 최대 포화축적기간을 제시하고자 실시하였다. 본 연구에 사용된 비육후기 거세한우는 30두로 각 급여기간(2개월: 평균 체중 677kg, 3개월: 평균 체중 610kg 및 4개월: 평균 체중 524kg)에 대한 대조구 및 Se-SMC 급여구로 나누어 처리구당 5두씩 배치하여 총 6처리구로 구분하여 실시하였다. 대조구와 Se-SMC 급여구에 사용된 실험사료는 0.1과 0.9ppm의 셀레늄을 각각 함유하였다. 각 급여기간이 종료되면 도축하여 후지와 간을 채취하였으며 이를 셀레늄 함량분석에 이용하였다. 그리고 혈중 셀레늄 농도 및 글루타치온 과산화효소활성을 분석하기 위하여 도축 시에 채혈을 실시하였다. 건물섭취량은 Se-SMC 급여 및 급여기간에 의하여 유의한 차이가 나타나지 않았다. 또한 일당증체량은 대조구 및 Se-SMC 급여구간 그리고 급여기간 간에 유의한 차이가 나타나지 않았을 뿐만 아니라 총증체량 또한 각 급여기간내 대조구와 Se-SMC 급여구 간에 유의한 차이가 나타나지 않았다. Se-SMC 급여는 혈중 셀레늄농도를 유의하게 증가시켰으나(P<0.001), 급여기간에 따른 혈중셀레늄 농도에는 유의한 영향을 미치지 않았다. 아울러 혈중 GSH-Px 활성은 혈중 셀레늄농도에서 나타난 양상과 유사한 경향을 나타내었고, 급여기간에 의한 효과는 나타나지 않았다. 후지내 셀레늄 함량은 급여기간(P<0.05)이 증가함에 따라, 그리고 Se-SMC 급여(P<0.001)에 의하여 유의하게 증가하였다. 하지만, Se-SMC의 3개월 급여군과 4개월 급여군 간에는 유의한 차이가 나타나지 않았다. 간내 셀레늄 함량은 Se-SMC의 급여기간이 증가함에 따라 다소 증가하는 경향을 나타내었으나 유의한 효과는 나타나지 않았다. 하지만, 동일한 급여기간 내 Se-SMC는 대조구에 비하여 유의하게 높았다(P<0.01).이상의 결과로부터 근육내 셀레늄 축적을 위한 적정 Se-SMC 급여기간은 후지와 간내 셀레늄 함량을 고려할 때 약 2 내지 3개월의 급여가 충분할 것으로 사료된다.

• Journal of Nutrition and Health
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• 제27권9호
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• pp.940-948
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• 1994

The present study was designed to determine if prenatal and postnatal Se nutriture affects Se concentration, glutathione peroxidase(GSHPx) activity and phospholipid distribution of the neonatal rat lung. Female SD rats were bred and fed a semipurified Se-deficient(0.04ppm, Se-) or a Se-adequate(0.5ppm, Se+) diet through pregnancy and lactation. On d 2 of lactation, maternal dietary Se had no significant effect on pulmonary Se concentration of pups. On d 16 of lactation, mean milk Se concentration in Se- dams was significantly lower than that in Se+ dams. Milk Se concentration was reflected on lung Se concentration and GSHPx activity of d 16 pups, which were dramatically decreased in Se- pups. In addition, pulmonary disaturated phosphatidyl choline/total phosphatidyl choline ratio was also significantly decreased in Se- pups, implying impaired function of pulmonary surfactant. These data indicate that adequate Se nutrition is important in the maturation of neonatal rat lungs.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.83-89
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• 1994

The effects of inorganic selenium (Se), selenate and selenite on Se balance levels in the different ruminal fluid fractions were studied using Japanese Corriedale wethers with an average body weight of 47 kg. A $3{\times}3$ Latin square design was used with three animal, three periods and three treatments. In each period, there was 7 d dietary adjustment followed by 5 d total collection of urine and feces. Ruminal fluid samples were obtained at 0, 1, 3, 5 and 7 h postprandially on the final day of the collection period. The three dietary treatments were: (1) without Se supplementation (control); (2) with Se supplement as sodium selenate; and (3) sodium selenite at a rate of 0.2 mg Se/kg dietary DM. The basal diet was timothy hay (Phleum pratense L.) fed 2% of body weight/d. Results indicated that Se balance were higher (p < 0.05) for those animals under supplementation than those animals under control. Overall data gathered showed a similar digestion balance of selenate and selenite in sheep. Inorganic Se, both selenate and selenite produced positive Se contents of the ruminal feed particles and protozoa. Bacterial Se increased (p < 0.05) on the first three hours post-prandially in Se supplemented diets. Gross ruminal fluid fraction, although there was improvement on their Se content under the supplemented diets, the changes were insignificant over the control. free inorganic Se and Se in soluble protein of the ruminal fluid were not significantly different for selenate and selenite. Most of the Se in the ruminal fluids of the animals under supplementation were insoluble, indicating the influence of rumen environments on Se bioavaliability.

• 한국식품영양과학회지
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• 제33권7호
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• pp.1206-1211
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• 2004

본 연구는 selenium binding yeast peptide의 식이내 첨가가 돼지의 생산성, 조직내 Se, 혈청내 GSH-Px의 활성 및 돈육의 품질에 미치는 영향을 확인하고자 실시하였다. 3원 교잡종(Duroc ${\times}$ Yorkshire ${\times}$ Landrace) 비육돈 80두를 공시하였으며 시험개시 시의 체중은82.88$\pm$1.23kg이었다. 시험설계는 옥수수-대두박 위주의 식이로서 처리한 대조구(CON：기초식이), 대조구 식이 내 selenium binding yeast peptide 물질을 0.05%(SY1. 대조구＋0.05% selenium binding yeast feptide), 0.1%(SY2： 대조구＋0.1% selenium binding yeast 울ptide) 및 0.2% 첨가한 구(SY3： 대조구＋0.2% selenium binding yeast peptide)로 4개 처리를 하여 처리 당 5반복, 반복 당4두씩 임의 배치하였다. 사양기간 동안의 성장률에 누는 selenium binding yeast peptide를 급여한 처리구가 대조구에 비하여 다소 높은 경향을 보였지만, 유의적인 차이는 보이지 않았다(p＞0.05). 명도를 나타내는 L＊-값은 SY2가 대조구와 SY1에 비하여 유의적인 차이를 보였다(p＜0.05). 적색도를 나타내는 a＊-값은 대조구가 다른 처리구와 비교하여 낮은 수치를 나타내었다(p＜0.05). 혈청 내 Se의 함량은 대조구가 516 mg/mL로 selenium binding yeast peptide를 급여한 처리구에 비하여 낮게 나타났지만 유의 적인 차이는 보이지 않았다(p＞0,05). 등심의 Se 함량은 대조구(0.008 $\mu$g/g)에 비해 SY2(0.021 $\mu$g/g) 및 SY3(0.031 $\mu$g/g)에서 유의적인 차이를 나타냈다(p＜0.05). 신장의 Se함량에서는 SY2와 SY3가 대조구 및 SY1과 비교하여 유의적으로 높게 나타났다(p＜0.05). 간에서는SY1이 대조구에 비하여 높게 나타났다(p＜0.05). 위의 결과를 종합하여 볼 때 비육돈 식이내 selenium binding yeast peptide의 첨가는 등심, 신장 및 간에서 많이 축적되는 것으로 보이며, 육색에도 영향을 미치는 것으로 보인다.

• 현장농수산연구지
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• 제5권1호
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• pp.36-56
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• 2003

Selenium(Se) is an essential trace element in the human body. Main function of this element is a catalytic part of antioxidant enzymes that protect cells against the attacks of free radicals that are produced during normal metabolism of the body. Se is also essential for normal function of the immune system and thyroid gland. It also appears to be a key nutrient in counteracting the development of virulence and inhibiting HIV(human immunodeficiency virus) progression to AIDS. It is also required for sperm motility and reduces the depression. Therefore, it is very meaningful that livestock producers generate Se-fortified animal products, such as Se-egg, Se-milk, Se-pork, Se-chicken and Se-beef from the point of producers as well as human heath. However, regulation on Se usage and Se-fortified food/feed is far from being clear in Korea even though Se should be carefully monitored because of its toxicity. Thus, one has to be aware of Se properties when designing Se-fortified animal products.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.984-991
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• 2006

Effects of spent composts of selenium-enriched mushroom (Se-SMC) on plasma glutathione peroxidase (GSH-Px) activity and selenium (Se) deposition in finishing Hanwoo (Bos taurus coreanae) steers were investigated. Twenty-five Hanwoo steers (average body weight = 613 kg, average age = 22 months) were allotted to treatments in five groups of five steers per pen for 12 weeks preceding slaughter. Treatments were SMC alone (CON; 0.1 ppm Se), 0.3 ppm (0.3 Se-SMC), 0.6 ppm (0.6 Se-SMC), 0.9 ppm (0.9 Se-SMC), and 0.9 ppm (sodium selenite; SENI) Se. During the experimental period, blood samples were taken to analyze Se concentrations and GSH-Px activities. Muscle and liver samples were collected for analyses of Se contents after slaughter. Dry matter intake and body weight gain were not affected by Se-SMC or sodium selenite supplementation. Selenium concentration in the whole blood and GSH-Px activity in plasma were linearly increased (p<0.01) with increasing levels of Se-SMC. The whole blood Se concentration of SENI treatment was significantly higher (p<0.05) than that of CON treatment from 4 weeks, whereas there was no significant difference in GSH-Px activities between both treatments at 8 and 12 weeks. Selenium content in the hind leg and liver increased linearly (p<0.05) with increasing levels of Se-SMC, but those of SENI treatments were not significantly different from CON treatments. These results suggested that Se in the Se-SMC was highly bioavailable to blood and tissues of ruminants, especially compared with Se in the sodium selenite. Therefore, Se-SMC might be used not only as an inexpensive way of providing Se for ruminants but also as another way of producing Se-fortified beef.

• 한국식품과학회지
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• 제4권2호
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• pp.61-71
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• 1972

Se은 비타민 E의 기능과 비슷한 기능을 가지고 있으므로 필수원소로 생각되나, 허용량을 조금만 넘으면 중독증을 일으키는 유해원소이다. 이러한 Se분석은 종래방법은 시료분해중 Se의 증발로 인한 손실과 시약의 오염으로 오는 오차로 분석이 힘들었다. 그러나 Se분석을 $^{77m}Se$(반감기 18초)의 핵종을 이용하여 비파괴 분석으로 간편하고 고감도로 미량을 신속하게 방사화 분석을 이용하여 분석할 수가 있다. 본 실험은 한국산 곡류, 야채, 과일, 건초, 육류, 해산물 등을 분석한 결과를 외국산 것과 비교한 결과를 발표한다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권5호
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• pp.730-746
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• 2015

Selenium plays an important role in boar nutrition via participating in selenoprotein synthesis. It seems likely that selenoproteins are central for antioxidant system regulation in the body. Se-dependent enzyme glutathione peroxidase (GSH-Px) is the most studied selenoprotein in swine production. However, roles of other selenoproteins in boar semen production and maintenance of semen quality also need to be studied. Boar semen is characterised by a high proportion of easily oxidized long chain polyunsaturated fatty acids and requires an effective antioxidant defense. The requirement of swine for selenium varies depending on many environmental and other conditions and, in general, is considered to be 0.15 to 0.30 mg/kg feed. It seems likely that reproducing sows and boars are especially sensitive to Se deficiency, and meeting their requirements is an important challenge for pig nutritionists. In fact, in many countries there are legal limits as to how much Se may be included into the diet and this restricts flexibility in terms of addressing the Se needs of the developing and reproducing swine. The analysis of data of various boar trials with different Se sources indicates that in some cases when background Se levels were low, there were advantages of Se dietary supplementation. It is necessary to take into account that only an optimal Se status of animals is associated with the best antioxidant protection and could have positive effects on boar semen production and its quality. However, in many cases, background Se levels were not determined and therefore, it is difficult to judge if the basic diets were deficient in Se. It can also be suggested that, because of higher efficacy of assimilation from the diet, and possibilities of building Se reserves in the body, organic selenium in the form of selenomethionine (SeMet) provided by a range of products, including Se-Yeast and SeMet preparations is an important source of Se to better meet the needs of modern pig genotypes in commercial conditions of intensive pig production.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2001년도 추계학술대회 논문집
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• pp.492-496
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• 2001

Due to their better photosensitivity in X-ray, the amorphous selenium based photoreceptor is widely used on the X-ray conversion materials. It was possible to control the charge carrier transport of amorphous selenium by suitably alloying a-Se with other elements(e,g. As, Cl). In this paper, We investigated dopants(As, Cl) composition rate to improve dark resistivity and transport properties of charge carrier in amorphous selenium using by direct X-ray conversion material. Alloying a-Se with As inhibits the recrystallization of a-Se but introduces undesirable deep hole traps. then doping with Cl(in the ppm range) compensates for the deep hole traps. We investigated their composition rate in various doping conditions and then obtained optimum dopant composition rate. The result was Se-As 0.3%-Cl 30 ppm and X-ray Sensitivity was 0.57 pc/pixel$.$mR at 137 $\mu\textrm{m}$ x 137 $\mu\textrm{m}$ Pixel area.

• Asian-Australasian Journal of Animal Sciences
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• 제11권3호
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• pp.265-267
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• 1998

Cattle in Alaska seemed to be tolerant to low blood selenium (Se) although arctic winter energy demands might exacerbate oxidative damage to tissues lacking protection by Se. The thermogenic properities of brown adipose tissue (BAT) and the long cold adaptation period for Alaskan cattle indicates that they might develop BAT. Eighteen mature beef cows with whole blood Se of< 19 ppb were used to examine possible relation-ships among Se deficiency, tissue lesions, and observable BAT. All cows were wintered on a diet low in Se and nine cows were given supplemental Se provided by intraruminal bolus. Blood Se was elevated (p < .05) from November through February by supplemental Se, but body weight and back fat thickness were unaffected (p < .10). Tissues were taken from two Se deficient cows, two cows supplemented with Se, and two cows given large doses of Se (300 mg by injection) four weeks before slaughter. Histopathological examination of 187 samples of fat (7 to 55 from each cow) showed no observable amount of BAT. Examination of other tissues showed no lesions attributable to Se deficiency.