• 제목/요약/키워드: Selective media

검색결과 332건 처리시간 0.027초

식품 유래 대장균군의 발색 배지 분리 효율 비교 (Comparative Evaluation of Selective Chromogenic Media for Coliforms Bacteria Isolated from Food)

  • 이다연;김희언;이진성;김근성;조용선
    • 한국식품위생안전성학회지
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    • 제31권3호
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    • pp.222-225
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    • 2016
  • 식품에서 대장균군을 효율적으로 분리하기 위해 대장균군 선택배지 5종: Chromocult Chromocult coliform agar (Merck), Hicrome coliform agar (Sigma), CHROMagar ECC, Brilliance E.coli/coliform medium (OXOID), endo agar (Merck)을 선별하여 식품 분리 대장균군 83종 및 표준균주 21종에 대해서 민감도와 특이도를 분석한 결과 Chromocult coliform agar와 HICrome coliform agar에서 94%도 민감도가 가장 높았으며, Brilliance E.coli/coliform medium는 93%, CHROMagar ECC는 92%, ENDO agar는 74%의 민감도를 나타냈다. Chromocult coliform agar와 HICrome coliform agar는 대장균군의 회수율도 높았다. 그러므로 Chromocult coliform agar와 HICrome coliform agar는 대장균군을 분리하는데 가장 효율적인 배지로 생각된다.

김치에서 젖산균의 선발 및 계수를 위한 선택배지 (Selective Media for Isolation and Enumeration of Lactic Acid Bacteria from Kimchi)

  • 이명기;박완수;강국희
    • 한국식품영양과학회지
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    • 제25권5호
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    • pp.754-760
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    • 1996
  • 일반적으로 사용되는 젖산균 선택 배지와 변화시킨 배지 중에 김치 젖산균 분리를 위한 선택배지는 Enterococcus속과 Pediococcus속 선별에 KF Streptococcus 배지가, Lactococcus속 선별에 pH 지시약을 첨가한 M-17배지가, Lnctobacillus속 선별에 m-LBS 배지가, Leuconostoc선별에 PES-3 배지가 우수하였다.

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염농도가 다른 새우젓에 존재하는 유해가능 세균의 초고압 살균 (High Hydrostatic Pressure Sterilization of Putrefactive Bacteria in Salted and Fermented Shrimp with Different Salt Content)

  • 목철균;송기태
    • 한국식품과학회지
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    • 제32권3호
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    • pp.598-603
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    • 2000
  • 열처리에 의한 식품의 성분변화 손실을 줄이기 위한 비열처리 기술중 하나로 새롭게 주목받고 있는 초고압 처리를 적용하여 저염발효 새우젓의 저장성을 증진시키고자 하였다. 염농도 8%로 $20^{circ}C$에서 6주간 발효한 새우젓의 Vibrio 선택배지 검출균은 $1.6{\times}10^3\;CFU/g$, Staphylococcus 선택배지 검출균은 $9.3{\times}10^3\;CFU/g$이었고 18%의 새우젓에서의 Vibrio 선택배지 검출균은 $1.4{\times}10^4\;CFU/g$, Staphylococcus 선택배지 검출균은 $1.7{\times}10^\;CFU/g$이었다. 새우젓을 압력 $3,500{sim}6,500$기압, 시간 $5{\sim}40$분의 범위로 초고압 살균하였을 때 압력과 처리시간이 증가할수록 살균효과가 증가되었으며 사멸패턴은 1차반응으로 확인되었다. 압력을 6,500기압으로 고정하고 10분 동안 처리하였을 때 유해가능 세균은 본 연구방법의 검출한계인 $10^2\;CFU/g$수준에서는 경출되지 않았다. Vibrio선택배지 검출균의 $D_p$값은 염농도 8%보다 18%에서 더 높았으며 Staphylococcus 선택배지 검출균은 18%보다 8%에서 더 높은 값을 보였다. Vibrio 선택배지 검출균과 Staphylococcus 선택배지 검출균 모두 염농도 8%에서의 $Z_p$값이 염농도 18% 경우보다 높은 값을 보였다. 초고압 공정은 저염발효 새우젓의 살균에 응용될 수 있었으며 최적 고압살균조건은 6,500기압에서 10분간 처리하는 것이었다.

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주요 식중독균 분리용 선택배지의 타당성 연구 (Evaluation of Selective Media for Isolation of Foodborne Bacteria)

  • 조서희;하지형;김근성;심영환;권기성;한정아;황인균;하상도;오덕환
    • 한국식품위생안전성학회지
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    • 제22권4호
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    • pp.388-394
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    • 2007
  • 본 연구는 현행 식품공전상 제시되어 있는 식중독균 분리배지의 평가를 위해 Salmonella spp., Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus의 5가지 식중독세균용 29가지 분리 선택배지를 Broth와 Food에서 시험하였다. Food는 닭고기, 쌀, 돼지고기, 고등어를 대상으로 5가지 식중독균을 접종후 균주의 recovery를 확인하였고. Broth는 5가지 식중독세균용 29가지 분리 선택배지를 사용하였다. Broth에서는 각 세정균에 대한 분리배지들 간에 유의적인 차이가 없었으나(P<0.05), Food에서는 그 차가 크지는 않았으나, 분리선택배지간의 분리능에 통계적 유의차가 존재하였다. 식품공전에 등재된 전통적인 분리 선택배지에서 분리된 균수가 Chromogenic 배지 등 새로운 선택배지에서 분리된 균수보다 많았다. 국제적으로 등재된 배지간의 선택성의 차이가 존재하였으며, 추후 다양한 배지가 식품공전에 등재되기 위해서는 추가 연구가 필요하다.

식품으로부터 쉬겔라 검출을 위한 분리배지 비교 (Comparison of Selective Media for Isolation and Detection of Shigella spp. from Foods)

  • 인예원;하수정;오세욱
    • 한국식품영양과학회지
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    • 제40권7호
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    • pp.1025-1031
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    • 2011
  • 본 연구는 식품으로부터 Shigella spp.를 검출하기 위해서 현재 사용되고 있는 분리배지를 대상으로 분리 성능을 비교하였다. MAC, SSA, DCA, XLD, HEA와 CHROMagar 총 6개의 분리배지를 대상으로 비교하였다. 일반 Shigella spp. 에 대한 성장은 MAC에서 TSA와 비슷한 수준 균수를 보였고 DCA에서 낮은 균수로 측정되었다. 소고기, 돼지고기, 새우를 대상으로 한 회수실험은 S. sonnei가 CHROMagar에서 성장이 유의적으로 높았으며(p<0.05) 양상추와 양배추에서는 S. sonnei가 SSA에서의 성장이 유의적으로 높게 나타났다(p<0.05). 식품 상재균은 소고기, 새우, 양배추에서 전체적으로 낮은 수준으로 측정되었으나 각 배지에서 유의적 차이는 없었다(p>0.05). 열 스트레스를 받은 S. sonnei는 MAC, SSA에서만 성장하였다. Enterobacteriaceae를 대상으로 한 선택성 측정 결과 HEA가 가장 높은 선택성을 나타내었다. Morganella spp.는 각 분리배지에서 Shigella spp.와 구분되지 못하였다. 실험에 사용된 배지는 각각의 조건에 따라 분리되는 특성이 상이하므로 식품에서 분리배지를 이용하여 Shigella spp.를 분리하고자 할 경우 분리 균의 상태에 따라 분리배지를 선택하여 사용하는 것이 바람직할 것으로 생각되었다.

Evaluation of Selective Media Containing Iron Source and Alpha-Glucosidase Substrates for Enterobacter sakazakii (Cronobacter spp.) Detection

  • Chon, Jung-Whan;Seo, Kun-Ho;Yim, Jin-Hyeok;Bae, Dongryeoul;Kim, Binn;Kim, Tae-Jin;Jeong, Dongkwan;Song, Kwang-Young
    • Journal of Dairy Science and Biotechnology
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    • 제39권1호
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    • pp.9-19
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    • 2021
  • Enterobacter sakazakii (Cronobacter spp.) causes meningitis, necrotizing enterocolitis, sepsis, and bacteremia in neonates and children and has a high mortality rate. For rapid E. sakazakii detection, various differential and selective media containing α-glucosidase substrates, such as 5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside (BCIG) or 4-methylumbelliferyl-α-D-glucoside (α-MUG), have been developed as only E. sakazakii exhibits α-glucosidase activity in the genus Enterobacter. However, Escherichia vulneris (family: Enterobacteriaceae) can also utilize α-glucosidase substrates, thereby resulting in false positives. Various iron sources are known to promote the growth of gram-negative bacteria. This study aimed to develop a selective medium containing α-glucosidase substrates for E. sakazakii detection that would eliminate false positives, such as those of E. vulneris, and to determine the role of iron source in the medium. Three previously developed (TPD) media, i.e., Oxoid, OK, and VRBG, and the medium developed in this study, i.e., NGTE, were evaluated using 58 E. sakazakii and 5 non-E. sakazakii strains. Fifty-four E. sakazakii strains appeared as fluorescent or chromogenic colonies on all four media that were assessed. Two strains showed colonies on NGTE medium and not on TPD media. In contrast, the remaining two strains showed colonies on TPD media and not on NGTE medium. None of the non-E. sakazakii strains showed fluorescent or chromogenic colonies on any of the evaluated media except E. vulneris, which showed colonies on TPD media and not on NGTE medium. This study demonstrated that the newly developed NGTE medium was not only equally efficient in promoting the growth of bacterial colonies when compared with the currently available media but also eliminated false positives, such as E. vulneris.

Study on Wave Absorption of 1D-/2D-Periodic EBG Structures and/or Metamaterial Layered Media as Frequency Selective Surfaces

  • Kahng, Sung-Tek
    • Journal of electromagnetic engineering and science
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    • 제9권1호
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    • pp.46-52
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    • 2009
  • This paper conducts a study on the frequency-dependent filtering and blocking effects of a variety of periodic structures, dubbed frequency selective surface(FSS). The periodic structures of interest are 1D and 2D repeated patterns of metal patches or slots sitting on the interface between the two different regions in the layered media which will show the capacitive or inductive behaviors and incorporated with the electromagnetic bandgap(EBG) geometry as another stratified media. Besides the normal substances so called double positive(DPS)-type in the layered media, metamaterials of double negative(DNG) are considered as layering components on the purpose of investigating the unusual electromagnetic phenomena. Frequency responses of transmission(absorption in terms of scattering) and reflection will be calculated by a numerical analysis which can be validated by the comparison with the open literature and demonstrated for the periodic structures embedding metamaterials or not. Most importantly, numerous examples of FSS will present the useful guidelines to have absorption or reflection properties in the frequency domain.

3D Filmmaking for User-Selective UHD Stereoscopic Media System: A Case Study on the Film The Old, the New and the Other

  • Cha, Minchol;Hamacher, Alaric;Simon, Sebastien
    • Journal of Multimedia Information System
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    • 제8권4호
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    • pp.277-284
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    • 2021
  • Despite skepticism about commercial potential, the stereoscopic 3D cinema is still a form that any filmmaker can choose to employ for its aesthetics and its immersive potential. Based on a haptic illusion, the stereoscopic media content requires a new perspective different from the principle of 2D media content in terms of creation and acceptance. This paper examines the technical and aesthetic issues of stereoscopic 3D film production from the perspective of today's emerging realistic and immersive media through a case study. One of the key factors for successful content creation and research and development in stereoscopic 3D cinema is the combination of artistic principles together with technical mastering of the new image technology. The purpose of this paper is to outline the principal challenges and research topics in stereoscopic 3D cinema through a case study of stereoscopic 3D pilot film production for the 'User-Selective UHD Stereoscopic Media Service Platform' of the ETRI (Electronics and Telecommunications Research Institute). This paper intends to examine stereoscopic 3D filmmaking workflow and production methodologies, as well as technical elements and aesthetic issues.

Effect of Reservoirs on Microbiological Water Qualities in a Drinking Water Distribution System

  • Lee Dong-Geun;Kim Sang-Jong;Park Seong-Joo
    • Journal of Microbiology and Biotechnology
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    • 제16권7호
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    • pp.1060-1067
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    • 2006
  • This study was undertaken to determine the effect of reservoirs on water quality and the distribution of pathogenic and indicator bacteria in a drinking water distribution system (total length 14km). Raw water, disinfected water, and water samples from the distribution system were subjected to physicochemical and microbiological analyses. Most factors encountered at each season included residual chloride, nitrate, turbidity, and phosphorus for heterotrophic bacterial distribution, and hardness, heterotrophic bacteria, sampling site, and DOC (dissolved organic carbon) for bacteria on selective media. No Salmonella or Shigella spp. were detected, but many colonies of opportunistic pathogens were found. Comparing tap water samples taken at similar distances from the water treatment plant, samples that had passed through a reservoir had a higher concentration of heterotrophic bacteria, and a higher rate of colony formation with 10 times as many bacteria on selective media. Based on the results with m-Endo agar, the water in reservoirs appeared safe; however, coliforms and opportunistic pathogenic bacteria such as Pseudomonas aeruginosa were identified on other selective media. This study illustrates that storage reservoirs in the drinking water distribution system have low microbiological water quality by opportunistic pathogens, and therefore, water quality must be controlled.

Microwave를 이용한 배지 융해와 공기 제거 (Usefulness of microwave to melt rehydrated media and to remove oxygen from anaerobic tube media)

  • 정윤섭;이귀녕;이삼열
    • 미생물학회지
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    • 제18권3호
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    • pp.148-152
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    • 1980
  • The microwave of 2450 MHz, generated by a household cooking oven, was evaluated for its applicability to melt various rehydrated media and to remove dissolved oxygen from tubed media for anaerobic culture. The effect on the sterilization of E. coli in selective media was also evaluated. The following results were obtained. 10 The microwave oven was useful in saving time for melting media and in eliminating heat and combustion gas from the laboratory, which were inevitable by-products in the conventional flame method. 2) Dissolved oxygen could be removed without boiling over by exposing the tubes of anaerobic culture medium after putting them in a wire basket in a beaker with water. 30 The count of E. coli during the melting of MacConkey and EMB agar were similar to those treated with open flame. The microwave treatment was not considered a possible mean to replace autoclaving even in these selective media.

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