• 대한기계학회논문집
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• 제17권7호
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• pp.1719-1730
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• 1993

This paper presents the theoretical natural frequencies of out-of-plane deformable ring based on the variables such as out-of-plane deflection, torsional rotation and shear rotation. Based on the same variables, a finite element eigen analysis is carried out by using the $C^0$-continuous, isoparametric element which has three nodes per element and three degrees-of-freedom at each node. Numerical experiments are peformed to find the integration scheme which produces accurate natural frequencies, natural modes and correct rigid body motion. The uniformly reduced integration and the selective reduced integration give more accurate numerical frequencies than the uniformly full integration, but the uniformly reduced integration produces incorrect rigid body motion while selective reduced integration does correct one. Therefore, the ring element based on the three variables which employes selective reduced integration is recommended to avoid spurious modes, to alleviate the error due to shear locking and to produce correct rigid body motion, simultaneously.

• 생명과학회지
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• 제28권11호
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• pp.1277-1284
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• 2018

본 연구에서는 효모염색체내에 다양한 유전자 발현 cassette를 도입하기 위해 Cre/loxP system을 가진 repeated yeast integrative plasmid (R-YIp)를 구축하였다. R-YIp는 반복적으로 형질전환체를 선별할 수 있는 selective marker (CgTRP1)와 loxP 서열, 그리고 integration을 위한 목적서열을 함유하고 있어 같은 염색체의 동일한 위치에 여러 개의 유전자 발현 cassette를 도입하는 것이 가능하다. 따라서 xylan/xylose 대사에 관련된 endoxylanase (XYLP), ${\beta}$-xylosidase (XYLB), xylose reductase (GRE3) 그리고xylitol dehydrogenase (XYL2)의 효모염색체내에 도입을 시도하였다. 먼저 XYLP, XYLB, GRE3그리고 XYL2 유전자의 효율적인 발현을 위한 promoter를 선별하기 위해 pGMF-GENE과 pAMF-GENE plasmid를 구축하였고, 각 유전자들의 발현에 GAL10 promoter가 적합함을 확인하였다. 다음으로 GAL10p-GENE-GAL7t cassette를 가진 pRS-GENE plasmid (R-YIp)를 구축하여, 반복적 integration 과정과 selective marker의 제거를 통해 각각의 R-YIps를 효모 7번염색체에 순차적으로 도입하였다. R-YIp system을 통해 효모염색체내에 도입된 유전자들은 모두 안정적으로 발현되었고, 활성형의 재조합효소를 생산함을 확인할 수 있었다. 따라서 다수의 외래유전자를 효모염색체내 도입함에 있어 selective marker와 숙주세포 선택의 한계를 R-YIp system을 통해 어느 정도 극복할 수 있을 것이라 기대한다.

• 한국미생물·생명공학회지
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• 제23권5호
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• pp.536-543
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• 1995

Multicopy integration vector is a very useful vector system in that they can be integrated into chromosomal DNA in several copies and stably maintained under non-selective conditions. To develop a multicopy integration vector system in the yeast Yarrowia lipolytica, P-type ribosomal DNA was cloned from Y lipolytica. A HindIII-BglII fragment of the cloned rDNA and a promoterless URA3 gene were inserted into pGEM1, generating multicopy integration vectors, pMIYL-1 and pMIYL-2. The rDNA fragment is for targeted homologous recombination between the vector and the chromosomal DNA of Y. lipolytica, and the promoterless URA3 gene is a defective selection marker for inducing multicopy integration. pMIYL-1 and pMIYL-2 have an unique restriction enzyme site, KpnI, and two unique restriction enzyme sites, KpnI and EcoRI, repectively, which can be used for targeting of the vectors into the rDNA of Y. lipolytica chromosomal DNA. After transformation of the vectors into Y. lipolytica, copy number and stability were analyzed by Southern hybridization. The vectors were found to be present in less than 5 copies per cell and were stably maintained during growth in non-selective media.

• 한국ITS학회 논문지
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• 제5권1호
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• pp.45-54
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• 2006

주파수 선택적 채널에서의 신호왜곡은 수신기에서의 채널추정을 어렵게 하여 펄스 타입의 UWB 시스템의 경우 동기 회로의 구현이 어렵다는 단점이 있다. 본 논문에서는 구현상 난점인 채널추정과 동기회로 설계를 간단히 기준 펄스를 함께 전송함으로써 해결하는 TR(Transmitted Reference), DTR(Differential TR) UWB을 분석하고 FSK 변조방법을 이용한 새로운 형태의 PRM(Pulse Repetition Modulation) UWB 방식을 제안한다. 기준 펄스를 이용한 통신의 수신기 구조인 Auto-correlation(AcR) 수신기가 잡음이 섞인 기준펄스를 이용하는 경우 성능저하 정도를 확률적으로 분석한다. 또한 SNR에 영향을 미치는 AcR 적분기의 적분구간에 대한 분석을 시뮬레이션을 통하여 분석한다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권4호
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• pp.1193-1215
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• 2021

The measurement of the key product quality index plays an important role in improving the production efficiency and ensuring the safety of the enterprise. Since the actual working conditions and parameters will inevitably change to some extent with time, such as drift of working point, wear of equipment and temperature change, etc., these will lead to the degradation of the quality variable prediction model. To deal with this problem, the selective integrated moving windows based principal component regression (SIMV-PCR) is proposed in this study. In the algorithm of traditional moving window, only the latest local process information is used, and the global process information will not be enough. In order to make full use of the process information contained in the past windows, a set of local models with differences are selected through hypothesis testing theory. The significance levels of both T - test and χ² - test are used to judge whether there is identity between two local models. Then the models are integrated by Bayesian quality estimation to improve the accuracy of quality variable prediction. The effectiveness of the proposed adaptive soft measurement method is verified by a numerical example and a practical industrial process.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1996년도 춘계학술대회 논문집
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• pp.181-185
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• 1996

Rapid prototyping is becoming an increasingly importnat techniuqe involved in the design cycles of modern industry. The majority of the rapid prototyping systems currently available use photo-reactive resins and waxes as the raw materials. The models produced by these systems often have relatively poor mechanical and physical properties and as such have a limited application to the production of advance prototypes but are excellently suited to the manufacture of engineering prototyes. This work identifies the need to produed near production grade advance prototypes from a variety of metals and a novel prototyping process based on the techniques of selective laser sintering and conventional machining is proposed. The integration of a carbon dioxide laser and a conventional machine tool to create the opto-mechanical by multi-layer sintering and some of the problems involved are also discussed.

• 한국표면공학회지
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• 제54권3호
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• pp.158-163
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• 2021

Some studies on electrochemical additive manufacturing of metals were summarized in this technical report, and development status of selective electrochemical 3D printing technology was introduced. In order to apply it to the PCB mass production process, essential considerations how to overcome the fundamental problems, such as the sizing, process sequence and PCB process design have been described.

• Journal of Plant Biotechnology
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• 제40권3호
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• pp.135-140
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• 2013

마커프리 형질전환 식물체 개발 방법의 하나인 무선발 형질전환은 선발마커 유전자를 이용한 도입유전자의 선발과정 없이, 연쇄중합반응(PCR)과 같은 직접 분석방법을 사용해 도입유전자가 도입된 형질전환 식물체를 개발하는 방법이다. 현재까지 감자, 담배, 알팔파, 밀, 땅콩 등 여러 작물에서 무선발 형질전환을 이용한 마커프리 형질전환체가 보고되었다. 그러나 대부분 무선발 형질전환체의 분석이 $T_0/T_1$세대에서의 PCR을 이용한 도입유전자 확인과 후대검정으로 수행되어, 유전자의 안정적 도입 확인을 위한 분자생물학적 특성평가 결과는 보고된 바 없다. 본 연구에서는 4계통의 무선발 형질전환 Bt벼에 대한 도입유전자 특성을 분자생물학적 방법으로 검정하여 유전자도입 위치, 도입유전자의 구조, 벡터 삽입여부 등의 분석 결과를 제시하였다.

• 한국콘텐츠학회논문지
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• 제5권6호
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• pp.87-96
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• 2005

본 논문은 동영상에서 ROI(region of interest)나 FOA(focus of attention)를 탐지하기 위한 방법으로써 여러 시각자극(stimuli)중 모션(motion) 정보를 사용한 선택적 주의 시스템을 소개한다. 2개 이상의 연속적인 프레임으로 구성되는 모션은 시간정보가 포함된 현실세계의 시각장면에서 시간적으로 현저함(temporal saliency) 정도를 나타내는 특징이라 할 수 있으며, 본 연구에서는 이러한 모션을 인간 뇌의 대뇌 시각피질에 존재하는 MT(middle temporal cortex) 영역에 존재하는 "이중 대립 수용야(double opponent receptive fields)"의 특성과 "노이즈 여과(noise filtration)" 기능에 대한 기존의 심리학적 연구 결과를 바탕으로 분석하고, 이렇게 분석된 결과를 MT에서의 "모션통합" 이론을 기반하여 통합한다. 이러한 과정을 통해 동영상에서 다른 주변의 영역과 현저히 다른 돌출 영역으로 정의되는 ROI또는FOA를 탐지할 수 있다. 제안하는 시스템의 성능분석을 위해 인간을 대상으로 한 심리학적인 실험을 추가적으로 수행하여 제안된 시스템을 통해 얻어진 결과와 비교함으로써, 본 시스템이 얼마나 타당성이 있는지 검증하였다.

• 아동학회지
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• 제33권1호
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• pp.41-62
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• 2012

This study explored the case of sandplay therapy for a 4th grade girl suffering from selective mutism. Her selective mutism apparently began following an extremely embarrassing experience in kindergarten. Her symptoms were a combination of symbiotic, reactive and passive- aggressive type behaviors. The goal of the therapy undertaken with this child was to enable her to express her repression and suppression, within a free and protective space during sandplay therapy. There were a total of 60 sessions of sandplay therapy. The client described the situations she had experienced in the first sandtray, by placing babies absent caring adults and food on the sandbox. She also placed baby fish away from their mother. In the mid-point of the sessions, she repeated her regressive behaviors by babbling like a baby and fought with snakes and monsters. In the final sessions, she showed integration and adaptation by engaging in snow play, expressing the union of opposites, placing blue and red mig and making a cross on the sand. This study showed the effectiveness of sandplay therapy since her selective mutism was lessened.