• 생명과학회지
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• 제30권3호
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• pp.239-249
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• 2020

감 꽃은 과실을 맺는 암꽃과 꽃가루를 내는 수꽃, 암술과 수술을 모두 가진 양성화가 있다. 관행 교배육종법으로 부본용 수꽃 신품종을 육성한다면 최종 선발까지 15년 정도의 장기간과 넓은 시험포장이 요구된다. 유묘기에 수꽃 착생 개체를 조기에 선발하여 육종효율을 증진하고자 꽃의 성표현을 알고 있는 주요 품종 88개체를 대상으로 Akagi 등(2014)이 보고한 수꽃 선발 마커들의 적용 효과를 검증하였다. OGI locus marker와 DISx-AF4S marker 적용 결과 각각 83품종, 72품종에서 꽃의 성표현과 분석 결과가 일치하였다. 육성품종인 '미감조생'을 모본으로 한 교배조합 등으로부터 나온 실생 2,509개체에 OGI locus marker 수꽃 선발 마커를 적용하여 890개체를 선발하였고, 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 1,186개체를 대상으로 꽃의 성표현과 마커 분석 결과를 비교하였을 때 401개체가 불일치한 것을 확인하였다(33.8%). 다양한 교배조합으로부터 나온 실생 889개체에 DISx-AF4S marker를 적용하여 분석한 결과 636개체를 선발하였다. 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 379개체를 대상으로 꽃의 성 표현과 마커 분석 결과를 비교하였고 247개체가 불일치한 것을 확인하였다(65.2%). 이상의 결과들을 종합하여 볼 때, 검토한 DISx-AF4S, OGI locus marker는 육종현장에서 활용하기에 유용하지 않을 것으로 판단된다.

• Reproductive and Developmental Biology
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• 제38권2호
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• pp.71-77
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• 2014

The specific genetic modification in porcine somatic cells by gene targeting has been very difficult because of low efficiency of homologous recombination. To improve gene targeting, we designed three kinds of knock-out vectors with ${\alpha}1,3$-galactosyltransferase gene (${\alpha}1,3$-GT gene), DT-A/pGT5'/neo/pGT3', DT-A/NLS/pGT5'/neo/pGT3' and pGT5'/neo/ pGT3'/NLS. The knock-out vectors consisted of a 4.8-kb fragment as the 5' recombination arm (pGT5') and a 1.9-kb fragment as the 3' recombination arm (pGT3'). We used the neomycin resistance gene (neo) as a positive selectable marker and the diphtheria toxin A (DT-A) gene as a negative selectable marker. These vectors have a neo gene insertion in exon 9 for inactivation of ${\alpha}1,3$-GT locus. DT-A/pGT5'/neo/pGT3' vector contain only positive-negative selection marker with conventional targeting vector. DT-A/NLS/pGT5'/neo/pGT3' vector contain positive-negative selection marker and NLS sequences in upstream of 5' recombination arm which enhances nuclear transport of foreign DNA into bovine somatic cells. pGT5'/neo/pGT3'/NLS vector contain only positive selection marker and NLS sequence in downstream of 3' recombination arm, not contain negative selectable marker. For transfection, linearzed vectors were introduced into porcine ear fibroblasts by electroporation. After 48 hours, the transfected cells were selected with $300{\mu}g/ml$ G418 during 12 day. The G418-resistant colonies were picked, of which 5 colonies were positive for ${\alpha}1,3$-GT gene disruption in 3' PCR and southern blot screening. Three knock-out somatic cells were obtained from DT-A/NLS/ pGT5'/neo/pGT3' knock-out vector. Thus, these data indicate that gene targeting vector using nuclear localization signal and negative selection marker improve targeting efficiency in porcine somatic cells.

• 산업경영시스템학회지
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• 제42권4호
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• pp.8-15
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• 2019

Korea's R&D investment has significantly increased in recent years and the quantitative outputs such as number of papers and patents have also increased with the investment. However, the quality of R&D outputs has not been fully addressed. In particular, quality of technical performance, such as the quality of patents, has attracted little attention. In this paper, a Data Envelopment Analysis (DEA) method was used to construct models for efficiency analysis of R&D investment, focused on quality of technical performance. Indices were proposed to analyze the quantitative and qualitative efficiencies of R&D investment. In order to effectively analyze R&D efficiencies, the measurement units of the input and output variables were standardized. Based on cases of livestock quarantine R&D projects of Korea, the quantitative and qualitative efficiencies of national R&D projects were analyzed and factors that would influence R&D efficiencies were identified. This paper suggests that both quantitative and qualitative efficiencies should be considered when measuring R&D efficiency. Also, it is recommended to carefully consider the characteristics of R&D projects during project selection stage.

• 한국물환경학회지
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• 제24권3호
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• pp.328-332
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• 2008

This research was about T-N removal efficiency of oxic reactor in which fixed media submerged from Membrane BNR(An+Ax+Ox) process. This experiment was implemented by using fixed media and changing DO concentration in the oxic reactor. Nitrification efficiencies of all modes were more than 98%. When no media was in the oxic reactor, T-N removal efficiency was only 47.4%, while it were shown from 60.3% to 67.4% with packed media, which indicated improved efficiency of 27~42%. The removal efficiencies of TCOD and BOD were more than 89%, 98% respectively, which could satisfy the guideline of advanced sewage reclamation by Department of Environment. When DO concentration in the packed oxic-media tank was 0.5~1.0 mg/L, T-N removal efficiency was low, which resulted from insufficient nitrification in the oxic reactor. Therefore, DO concentration of bulk solution needs to be kept more than 1.0 mg/L to induce higher nitrification efficiency in the reactor in which media was submerged. Also, the selection of DO concentration is important to prevent media from being clogged.

• Korean Chemical Engineering Research
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• 제55권4호
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• pp.530-534
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• 2017

Chitosan is a promising flocculant for microalgae harvesting, but its scale-up application is not economically supported yet. Low solubility of chitosan in microalgae suspension demands high dosage (as a flocculant) to destabilize the cells, and thus, increases the cost of microalgae harvesting. This study identifies efficient solvents for the chitosan, and optimizes the concentration of solvents and chitosan dose to improve the harvesting efficiency. Chitosan was dissolved in different acids, and subsequently used as a flocculant. The flocculant efficacy was measured in terms of harvesting efficiency and reduction in chemical oxygen demand (COD) of the microalgae suspension. It was found that chitosan dissolved in 0.05 M HCl showed the highest harvesting efficiency ($89{\pm}0.87%$) at only 30 mg/L of dosage. In comparison, 270 mg/L of $FeCl_3{\cdot}6H_2O$ was required to attain $86{\pm}0.083%$ of the harvesting efficiency. $H_2SO_4$ dissolved chitosan required high flocculant dose (150 mg/L) and resulted in relatively low harvesting efficiency ($77{\pm}0.11%$). It was concluded that the efficacy of chitosan is solvent dependent, and the selection of proper solvent can decrease the dosage requirement for microalgae harvesting.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권2호
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• pp.628-649
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• 2017

Security and resource-saving are both demands of the fifth generation (5G) wireless networks. In this paper, we study the secrecy spectrum efficiency (SSE) and secrecy energy efficiency (SEE) of a K-tier massive multiple-input multiple-output (MIMO) enabled heterogeneous cellular network (HetNet), in which artificial noise (AN) are employed for secrecy enhancement. Assuming (i) independent Poisson point process model for the locations of base stations (BSs) of each tier as well as that of eavesdroppers, (ii) zero-forcing precoding at the macrocell BSs (MBSs), and (iii) maximum average received power-based cell selection, the tractable lower bound expressions for SSE and SEE of massive MIMO enabled HetNets are derived. Then, the influences on secrecy oriented spectrum and energy efficiency performance caused by the power allocation for AN, transmit antenna number, number of users served by each MBS, and eavesdropper density are analyzed respectively. Moreover, the analysis accuracy is verified by Monte Carlo simulations.

• 한국지능시스템학회논문지
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• 제17권6호
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• pp.725-730
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• 2007

Otsu의 임계값 결정법, Huang와 Wang의 임계값 결정법 등을 포함한 그레이 레벨 히스토그램에 기반한 임계값 결정법은 영상처리 분야에서 널리 사용되어져 왔다. 이들 기법들은 그 효용성이 뛰어남에도 불구하고 하나의 임계값이 아닌 다중 임계값을 추출하는 경우 많은 연산 시간이 소요되는 단점을 가지고 있다. 즉, 임계값의 개수가 늘어남에 따라 연산 복잡도 역시 기하급수적으로 증가하게 된다 본 논문에서는 가우시안 함수를 이용하여 그레이 레벨간의 상관관계를 측정하고, 가우시안 분포함수와 그레이 레벨의 히스토그램을 결합한 가우시안형 유한 혼합 분포를 이용하여 연산 복잡도가 단순하며 효용성 있는 임계값 결정법을 제안한다. 다수의 영상에 제안한 기법을 적용한 모의실험을 통하여 효용성을 확인하고, Otsu의 임계값 결정법과 제안한 기법의 연산 복잡도 비교를 통해서 제안한 임계값 결정법의 효율성을 보인다.

• 정보처리학회논문지D
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• 제9D권5호
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• pp.897-902
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• 2002

소프트웨어 기술의 급속한 발전으로 컴포넌트 개발방법론은 많이 연구되었으나 컴포넌트의 품질을 구매자 관점에서 종합적이고 체계적으로 비교 및 평가하는 선정기법에 대한 연구가 미약한 상황이다. 지금까지 대부분의 컴포넌트 품질평가 방법은 객체지향의 척도를 기반으로 한 개발자 관점의 평가 방법이었으나 본 논문에서는 구매자 관점에서 최적의 상용컴포넌트를 선정하기 위한 기법으로 MCDM(Multiple Criteria Decision Making)기법을 활용한 4단계 선정 절차와 평가기준을 제시하였다. 상용컴포넌트의 품질은 국제표준(IS0/IEC 9126)에서 규정하고 있는 6가지 중요한 품질 특성 중에서 기능성, 효율성, 사용성을 고려하였으며 현재 인터넷상에서 판매하고 있는 상용 EJB 컴포넌트들에 대한 실증적 분석을 통하여 본 논문에서 제시한 상용컴포넌트 선정기법이 최적의 상용컴포넌트를 선택하는데 적용 가능한 것임을 보여준다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권11호
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• pp.5276-5298
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• 2019

Internet of Things (IoT) based sensor networks have gained unprecedented popularity in recent years. With the exponential explosion of the objects (sensors and mobiles), the bandwidth and the speed of data transmission are dwarfed by the anticipated emergence of IoT. In this paper, we propose a novel heterogeneous IoT model integrated the power line communication (PLC) and WiFi network to increase the network capacity and cope with the rapid growth of the objects. We firstly propose the mean transmission delay calculation algorithm based the 3D Markov chain according to the multi-priority of the objects. Then, the attractor selection algorithm, which is based on the adaptive behavior of the biological system, is exploited. The combined the 3D Markov chain and the attractor selection model, named MASM, can select the optimal path adaptively in the heterogeneous IoT according to the environment. Furthermore, we verify that the MASM improves the transmission efficiency and reduce the transmission delay effectively. The simulation results show that the MASM is stable to changes in the environment and more applicable for the heterogeneous IoT, compared with the other algorithms.

• 한국정보통신학회논문지
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• 제21권3호
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• pp.543-548
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• 2017

Epidemic 라우팅 프로토콜 기반의 메시지 확산 방식은 메시지를 수신한 기기들이 모두 릴레이를 수행하기 때문에 기기들의 전송 커버리지 중복으로 인한 메시지 중복 수신 문제가 빈번하게 발생한다. 따라서 본 논문에서는 이러한 문제점을 완화하기 위해 전송 범위 내에 존재하는 기기들 중 최외곽에 위치한 k개의 기기들만이 메시지 릴레이를 수행하는 최외곽 k개의 릴레이 선택 알고리즘을 제안하고 기존방식과 성능 비교분석을 수행한다. 포함 확률과 총 수신 메시지의 수 그리고 전송 효율에 대한 시뮬레이션 결과를 통해 제안 방안의 우수성을 증명한다.