• KSBB Journal
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• v.31 no.1
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• pp.33-39
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• 2016

Developing the method for the selection of animal cell line producing therapeutic monoclonal antibody (mAb) is invaluable as its market is rapidly growing. Although the quality of produced mAb is as important as quantity, however there is no method developed for the selective screening of cell lines on the basis of both quantity and quality. From recent reports, the ratio of light and heavy chain mRNAs of mAb in the cell is a key parameter for the indication of product quality. Therefore, it is obvious that developing the novel method that can detect both light and heavy chain mRNAs in single live cell will provide unprecedented opportunities in bio-industry. Here, we have constructed oligonucleotide probes, molecular beacons for the detection of light or heavy chain mRNAs, respectively, in the live cells producing mAbs. Both beacons showed increased fluorescent intensity after transient transfection of plasmid expressing mAbs analyzed by fluorometer. Flow cytometric analysis clearly demonstrated that both molecular beacons can simultaneously detect the expression of light and heavy chain mRNAs of mAb in the same cell. The technique described in the thesis provides the new direction and concept for developing the method for the smart selection of cell lines producing recombinant proteins including therapeutic mAbs.

• Journal of the Korean Society of Safety
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• v.12 no.2
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• pp.17-21
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• 1997

The fatigue crack propagation rate has so far been measured by the effective stress intensity factor range Δ K/sub eff/. But no research has been done to study how much the crack opening ratio influences the expectation of the fatigue crack propagation. Therefore, this paper evaluates of the crack opening ratio on the fatigue crack propagation life by using the method of parameter sensitivity study. Summarizing the result ; ( 1 ) When the crack opening ratio U' is high, the effect of U' gets larger than any other except for the material factor m. But when U' is low, the effect is also diminished. Therefore, the selection of the lowest value possible is desirable in the evaluation of life. (2) When the value of the material factor m is high, the effect of crack opening ratio U' is increased, at the same time the effect of the other parameters also increased wholly. The effect of material factor m itself on life is high, but in case the material factor m is high, that the effect of each parameter on life get higher is unique. In designing, better attention to the material selection should be drawn. (3) In case the stress ratio R gets smaller, the effect of crack opening ratio U' is unchangeable. But the effect of R itself remarkably decreases, and the effect of material factor m somewhat decreases.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.1
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• pp.43-52
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• 2021

According to several seismic design standards, a ground motion time history should be selected similar to the design response spectrum, or a ground motion time history should be modified by matching procedure to the design response spectrum through the time-domain method. For the response spectrum matching procedure, appropriate seed ground motions need to be selected to maintain recorded earthquake accelerogram characteristics. However, there are no specific criteria for selecting the seed ground motions for applying this methodology. In this study, the characteristics of ground motion time histories between seed motions and spectral matched motions were compared. Intensity measures used in the design were compared, and their change by spectral matching procedure was quantified. In addition, the seed ground motion sets were determined according to the response spectrum shape, and these sets analyzed the response of nonlinear and equivalent linear single degrees of freedom systems to present the seed motion selection conditions for spectral matching. As a result, several considerations for applying the time domain spectral matching method were presented.

• Journal of Korean Society of Forest Science
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• v.107 no.2
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• pp.193-204
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• 2018

This study was carried out to select the optimal method of competition index computation according to the competitor selection methods and distant-dependent competition index models, and to analyze the characteristics of competition indices in terms of thinning intensity and tree density targeting Pinus densiflora, Pinus koraiensis, and Larix kaempferi, which are the major coniferous species in Korea. Data was the re-investigated tree information from 240 permanent plots of 80 sites in the stands of P. densiflora, P. koraiensis, and L. kaempferi, which were located in the national forest of Gangwon and North Gyeongsang provinces. The number of subject trees with competition index calculated were 1126 trees for P. densiflora, 4093 trees for P. koraiensis, and 3399 trees for L. kaempferi. For the best competition index computation method, three kinds of competitor selection methods were considered: basal area factor, angle of height, angle of height to crown base. Also, six kinds of competition index models were compared: Lorimer, Martin-EK, Braathe, Heygi, Daniels, and Modified Daniels, which was developed in this study. Correlation coefficient was the best when the competitor selection method of basal area factor $4m^2/ha$ and the competition index model of Modified Daniels were used, and thus, it was selected as the best method for computing competition index. According to the best method by stand characteristics, competition index decreased in all species as thinning intensity was high and tree density was low.

• Journal of the Korea Society of Computer and Information
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• v.16 no.7
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• pp.149-156
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• 2011

This paper proposes the effective edge selection algorithm for the rapid processing time and low memory usage of efficient graph-based image segmentation on mobile device. The graph-based image segmentation algorithm is to extract objects from a single image. The objects are consisting of graph edges, which are created by information of each image's pixel. The edge of graph is created by the difference of color intensity between the pixel and neighborhood pixels. The object regions are found by connecting the edges, based on color intensity and threshold value. Therefore, the number of edges decides on the processing time and amount of memory usage of graph-based image segmentation. Comparing to personal computer, the mobile device has many limitations such as processor speed and amount of memory. Additionally, the response time of application is an issue of mobile device programming. The image processing on mobile device should offer the reasonable response time, so that, the image segmentation processing on mobile should provide with the rapid processing time and low memory usage. In this paper, we demonstrate the performance of the effective edge selection algorithm, which effectively controls the edges of graph for the rapid processing time and low memory usage of graph-based image segmentation on mobile device.

• Journal of Korea Water Resources Association
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• v.55 no.10
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• pp.775-784
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• 2022

In this study, to determine the optimal order of the full-logged I-D-F polynomial equation, which is mainly used to calculate the probable rainfall over a temporal rainfall duration, the probable rainfall was calculated and the regression coefficients of the full-logged I-D-F polynomial equation was estimated. The optimal variable of the polynomial equation for each station was selected using a stepwise selection method, and statistical significance tests were performed through ANOVA. Using these results, the statistically appropriately calculated rainfall intensity equation for each station was presented. As a result of analyzing the variable selection outputs of the full-logged I-D-F polynomial equation at 9 stations in Gyeongbuk, the 1^st to 3^rd order equations at 6 stations and the incomplete 3^rd order at 1 station were determined as the optimal equations. Since the 1^st order equation is similar to the Sherman type equation and the 2^nd order one is similar to the general type equation, it was presented as a unified form of rainfall intensity equation for convenience of use by increasing the number of independent variables. Therefore, it is judged that there is no statistical problem in considering only the 3^rd order polynomial regression equation for the full-logged I-D-F.

• Korean Journal of Agricultural Science
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• v.39 no.3
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• pp.407-412
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• 2012

Soil texture has an important influence on agriculture such as crop selection, movement of nutrient and water, soil electrical conductivity, and crop growth. Conventionally, soil texture has been determined in the laboratory using pipette and hydrometer methods requiring significant amount of time, labor, and cost. Recently, in-situ soil texture classification systems using optical diffuse reflectometry or mechanical resistance have been reported, especially for precision agriculture that needs more data than conventional agriculture. This paper is a part of overall research to develop an in-situ soil texture classification system using image processing. Issues investigated in this study were effects of sensor travel speed and light source and intensity on image quality. When travel speed of image sensor increased from 0 to 10 mm/s, travel distance and number of pixel were increased to 3.30 mm and 9.4, respectively. This travel distances were not negligible even at a speed of 2 mm/s (i.e., 0.66 mm and 1.4), and image degradation was significant. Tests for effects of illumination intensity showed that 7 to 11 Lux seemed a good condition minimizing shade and reflection. When soil water content increased, illumination intensity should be greater to compensate decrease in brightness. Results of the paper would be useful for construction, test, and application of the sensor.

• Journal of Wetlands Research
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• v.22 no.1
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• pp.1-7
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• 2020

Non-point pollution adversely affects the water system and its influence is increasing. In order to manage such nonpoint source pollution, the government has conducted studies on LID (Low Impact Development) facilities and various efficiency evaluations. In this study, the actual installed permeable block facility among the various LID facilities was analyzed the effluent reduction rate, the residual rainfall analysis, the runoff duration time and the reduction rate of the maximum inflow and outflow for the rainfall runoff control and the results were compared the other facilities. The analysis results show that the reduction efficiency is high in order of impermeable block, filter type permeable block, and clearance type permeable block, and the graph showing the relationship between the rainfall intensity and the runoff duration time is presented. This graph can be helpful in the design of facilities such as the facility capacity selection according to the reproduction period of the permeable block facility similar to this.

• Journal of the Korean Society for Marine Environment & Energy
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• v.16 no.4
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• pp.263-267
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• 2013

In this research, a novel optical measuring methodology for the measurement of oil thickness in seawater is suggested by evaluating the light absorption which is occurred in the process of penetrating through oil layer on seawater. Laser having monochromatic wave is used as a light source and photodiode which can convert the intensity of the light into an electrical signal is applied to measure the intensity of the penetrating light through the oil-water mixtures. In the experiment, bunker C and lubricating oil are used, and three different lasers having different wavelengths are applied and compared for the selection of an optimal light source. As a result, it is observed that in the case of blue laser, the intensity of the light on the optical sensor decreases with an increase in the oil thickness. Through this relation, both the presence of oil and the thickness of oil can be determined.

• The Korean Journal of Community Living Science
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• v.27 no.3
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• pp.351-369
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• 2016

Individual lifestyle and eating habits have changed rapidly due to the evolution of society. Especially, climate change caused by industrialization has influenced society, with the result that today's consumers perceive sustainability to be an important value. The purposes of this study were to segment consumers on the basis of their food-related lifestyle and to explore climate-friendly food consumption behavior by considering factors such as moral intensity, propensity for disgust with meat as well as consumer characteristics. The results of this study were as follows: first, consumers were segmented into three groups(high involvement, low involvement, convenience oriented). Consumers in the high involvement group presented a higher level of moral intensity and climate-friendly food consumption behavior than the other groups. Furthermore, factors influencing the selection of climate-friendly food were found to differ according to the type of consumer. Consumers in the high involvement group were found to be significantly influenced by age, concentration of effect, the morality of eating meat and meat texture, while consumers in the low involvement were found to be significantly influenced by social consensus and the morality of eating meat. Finally, consumers in the convenience oriented group for food life were found to be significantly influenced by age, harm perception, and the morality of eating meat.