• Title/Summary/Keyword: Selection Intensity

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Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development

  • Park, Jun-Kyung;Lee, Seung-Hwan;Lee, Jang-Hoon;Han, Songhee;Kang, Hunseung;Kim, Jin-Cheol;Kim, Young Cheol;McSpadden Gardener, Brian
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.144-153
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    • 2013
  • Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

Effects of preselection of genotyped animals on reliability and bias of genomic prediction in dairy cattle

  • Togashi, Kenji;Adachi, Kazunori;Kurogi, Kazuhito;Yasumori, Takanori;Tokunaka, Kouichi;Ogino, Atsushi;Miyazaki, Yoshiyuki;Watanabe, Toshio;Takahashi, Tsutomu;Moribe, Kimihiro
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.2
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    • pp.159-169
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    • 2019
  • Objective: Models for genomic selection assume that the reference population is an unselected population. However, in practice, genotyped individuals, such as progeny-tested bulls, are highly selected, and the reference population is created after preselection. In dairy cattle, the intensity of selection is higher in males than in females, suggesting that cows can be added to the reference population with less bias and loss of accuracy. The objective is to develop formulas applied to any genomic prediction studies or practice with preselected animals as reference population. Methods: We developed formulas for calculating the reliability and bias of genomically enhanced breeding values (GEBV) in the reference population where individuals are preselected on estimated breeding values. Based on the formulas presented, deterministic simulation was conducted by varying heritability, preselection percentage, and the reference population size. Results: The number of bulls equal to a cow regarding the reliability of GEBV was expressed through a simple formula for the reference population consisting of preselected animals. The bull population was vastly superior to the cow population regarding the reliability of GEBV for low-heritability traits. However, the superiority of reliability from the bull reference population over the cow population decreased as heritability increased. Bias was greater for bulls than cows. Bias and reduction in reliability of GEBV due to preselection was alleviated by expanding reference population. Conclusion: Cows are easier in expanding reference population size compared with bulls and alleviate bias and reduction in reliability of GEBV of bulls which are highly preselected than cows by expanding the cow reference population.

Buffer Intensity of Ammonia and MPA in Water-Steam Cycle of PWRs (가압경수로 원전 물-증기 순환영역에서 암모니아와 MPA의 완충세기)

  • Rhee, In-H.;Ahn, Hyun-Kyoung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.7
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    • pp.2708-2712
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    • 2010
  • Amines, ammonia or 3-methoxypropylamine (MPA), are used to maintain the optimized pH for the prevention of corrosion in the secondary side of Pressurized Water Reactors (PWRs). They are differently dissociated as a function of temperature which is not same in each location of the water-steam cycle. pH at the operation temperature depends on temperature of fluid and equilibrium constants of water and amines. Thus, every amine provides the different pH in the entire secondary side so that pH is not only the sufficient parameter in corrosion control. The secondary parameter, i.e., buffer intensity, is the ability to maintain a stable pH when $H^+$ are added or removed due to the ingress of impurities or the reaction of corrosion. The buffer intensity is necessary to provide the selection criteria for the best pH control agent for secondary side and the basic understanding of the reason why the flow-accelerated corrosion(FAC) rate may demonstrate the bell-shape curve over temperature. The buffer intensities of ammonia and MPA were reviewed over the entire operation temperature of PWRs. The sufficient buffer intensity is provided for the inhibition of corrosion by ammonia in low temperature $(25{\sim}100^{\circ}C)$ and by DMA in high temperature $(150{\sim}250^{\circ}C)$. In terms of buffer intensity, i) the best pH control agent is an amine with $pK_a(T)$ range of pH(T)- $1{\leq}pK_a(T){\leq}pH(T)$ + 0.5 and ii) the amine solution should have sufficient buffer intensity, ${\beta}$ to inhibit corrosion, and iii) FAC rate may be maximum at the temperature, where ${\beta}_B/{\beta}$ ratio is lowest.

SHADE ANALYSIS OF ANTERIOR TEETH USING DIGITAL SHADE ANALYSIS SYSTEM (Digital Shade Analysis System을 이용한 전치부의 색조 특성에 관한 연구)

  • Kim Hee-Eun;Cho In-Ho;Lim Ju-Hwan;Lim Hun-Song
    • The Journal of Korean Academy of Prosthodontics
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    • v.41 no.5
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    • pp.565-581
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    • 2003
  • Statement of problem : A scientific examination and understanding of overall aspects of the natural dentition is the first step involved in making a satisfactory selection in the shade of an aesthetic prosthesis. Proper natural shade selection of the prosthetic restoration that is in harmony with the remaining dentition is as important aesthetically, as harmony of form and function in the anterior dentition. Clinically, the most commonly applied method of shade selection has been visual, but because of the subjective nature inherent to this method, shade selection results are variable and can be influenced by such factors as the technician, the type of shade guide used, and the type and intensity of the lighting. Purpose : The purpose of this study was to develop a more objective and scientific approach to examining and understanding the shade of teeth, which has in turn lead to the development of a number of shade analysis devices that present a more objective method of shade analysis. Material and Method : In this study, the shades of healthy anterior teeth were examined and analyzed using the recently developed digital shade analysis of the $ShadeScan^{TM}$ System. The study examined 80 individuals in their twenties, 40 males and 40 females, presenting 6 healthy, unrestored maxillary anterior teeth. Tooth brushing and oral prophylaxis were performed prior to evaluation. The ShadeScan handpiece was used to acquire images of the 6 maxillary anterior teeth. These images were analyzed using the Vita/Classical mode of the $ShadeScan^{TM}$ Software, and shade maps of each tooth were acquired and divided into cervical, middle, and incisal thirds. The shade distribution of each third, left and right symmetry, and gender differences were investigated and analyzed. Results : The results of the study are as follows : 1. An overwhelming majority of the examined teeth were found to possess shades belong to Group A, with the greatest variations occurring at the middle and cervical thirds of the maxillary central and lateral incisors, in both male and female subjects. 2. Canines of both male and female subjects showed left and right symmetry with uniform shade distribution of A4 and C4, while the lateral and central incisors showed left-right symmetry of the incisal 1/3 with a uniform shade distribution of A2 and A3 shades 3. No significant differences in shade distribution were seen between genders in maxillary canines, whereas maxillary central and lateral incisors showed differences at the middle and cervical thirds between male and female subjects The results of this study show that with the exception of maxillary canines, maxillary anterior teeth display a diverse shade distribution as well as gender differences. Conclusion : Clinically, when making a shade selection using the existing shade guide, one must consider the fact that even a single tooth consists of a variety of shades. The results of this study show that when selecting a shade from a number of groups is difficult, shades from A group are the most consistent with the natural shade or maxillary anterior teeth.

High-Order Surface Gradient Coil Design Using Target Field Approach

  • Lee, J.K.;Yang, Y.J.;Jeong, S.T.;Choi, H.J.;Cho, Z.H.;Oh, C.H.
    • Journal of Biomedical Engineering Research
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    • v.17 no.1
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    • pp.19-24
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    • 1996
  • The purpose of this paper is to design high-order (or radial) surface gradient coil (SGC), which can provide multi-dimensional spatial selection. Although the spatial Selection with High-Order gradienT (SHOT) can provide a 2-D selection with only one selective RF pulse, the high-order gradient pro- duced by conventional cylindrical-shape coils has not been clinically useful due to the large selection size caused by the limited radial gradient intensity. However, by using the proposed high-order SGCs located near the imaging region, the size of volume selection can be reduced to a clinically useflll size of 1-2 cm in diameter by applying stronger radial gradient field with much less gradient driving power. So far radial SGCs have been designed by using the field component method and may cause distortion in the selection shapes. In this paper, by using the target field approach for the coil design, selected volumes became almost circular. A 40 cm-by-40 cm $z^2$_surface gradient coil has been designed and implemented by using the target field approach. Phantom and volunteer studies have been performed Experimental results using spatially localized MRI show good agreement to the theoretically predicted behavior.

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The Characteristics of Swirl Spray Combustion in Gas Turbine Combustor (가스터빈 연소기내의 선회분무연소 특성)

  • Hong, Jeong-Gu;Kim, Hyeok-Ju
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.8
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    • pp.2721-2730
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    • 1996
  • The present study conducted experimental study of spray combustion to investigate the effect of the inlet conditions of fuel and air on the flame structure, the flame stability and the characteristics of emission in the can-type model of a gas turbine combustor. In the experiment, the diameter of fuel droplet was measured using Malvern particle size analyser and temperatures in the combustion chamber were measured with R-type shielded thermocouple. In addition, flame structure was taken picture with camera and analysed. Gas analyser was also used to analyse the concentration of each components of exhausting gas. The experimental results showed that the flame condition was optimal with swirl number, 0.63 and equivalence ratio, 0.5 for controlling the flame stability, the combustion temperature and the NOx concentration. The present study concluded that both the flame structure and the emission formation were strongly affected by the swirl intensity, which selection was found as an important parameter for either stabilizing flame or lowering the quantity of NOx.

Gross tumor volume dependency on phase sorting methods of four-dimensional computed tomography images for lung cancer

  • Lee, Soo Yong;Lim, Sangwook;Ma, Sun Young;Yu, Jesang
    • Radiation Oncology Journal
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    • v.35 no.3
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    • pp.274-280
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    • 2017
  • Purpose: To see the gross tumor volume (GTV) dependency according to the phase selection and reconstruction methods, we measured and analyzed the changes of tumor volume and motion at each phase in 20 cases with lung cancer patients who underwent image-guided radiotherapy. Materials and Methods: We retrospectively analyzed four-dimensional computed tomography (4D-CT) images in 20 cases of 19 patients who underwent image-guided radiotherapy. The 4D-CT images were reconstructed by the maximum intensity projection (MIP) and the minimum intensity projection (Min-IP) method after sorting phase as 40%-60%, 30%-70%, and 0%-90%. We analyzed the relationship between the range of motion and the change of GTV according to the reconstruction method. Results: The motion ranges of GTVs are statistically significant only for the tumor motion in craniocaudal direction. The discrepancies of GTV volume and motion between MIP and Min-IP increased rapidly as the wider ranges of duty cycles are selected. Conclusion: As narrow as possible duty cycle such as 40%-60% and MIP reconstruction was suitable for lung cancer if the respiration was stable. Selecting the reconstruction methods and duty cycle is important for small size and for large motion range tumors.

Hierarchical Organ Segmentation using Location Information based on Multi-atlas in Abdominal CT Images (복부 컴퓨터단층촬영 영상에서 다중 아틀라스 기반 위치적 정보를 사용한 계층적 장기 분할)

  • Kim, Hyeonjin;Kim, Hyeun A;Lee, Han Sang;Hong, Helen
    • Journal of Korea Multimedia Society
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    • v.19 no.12
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    • pp.1960-1969
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    • 2016
  • In this paper, we propose an automatic hierarchical organ segmentation method on abdominal CT images. First, similar atlases are selected using bone-based similarity registration and similarity of liver, kidney, and pancreas area. Second, each abdominal organ is roughly segmented using image-based similarity registration and intensity-based locally weighted voting. Finally, the segmented abdominal organ is refined using mask-based affine registration and intensity-based locally weighted voting. Especially, gallbladder and pancreas are hierarchically refined using location information of neighbor organs such as liver, left kidney and spleen. Our method was tested on a dataset of 12 portal-venous phase CT data. The average DSC of total organs was $90.47{\pm}1.70%$. Our method can be used for patient-specific abdominal organ segmentation for rehearsal of laparoscopic surgery.

The Investigation of Magnetic Material Characteristic for Solenoid Valve Development (솔레노이드 밸브 개발을 위한 자성소재 특성 조사)

  • Kim, Byung-Hun;Yi, Moo-Keun;Kwon, Oh-Sung;Han, Sang-Yeop
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.817-820
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    • 2011
  • The solenoid valve is an electro-mechanical device that converts electrical energy into mechanical motion. The magnetic field of solenoid is very closely related to the number of coil winding, the intensity of current and the characteristic of magnetic material. There are disadvantages that the weight and size of valve increase, as increasing the number of coil winding, the intensity of current to augment the magnetic force. Therefore, the selection of magnetic material is very important to reduce the weight and size of solenoid valve.

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A Study on Application of the Photo Detector for Electromagnetic Fuel Injection System of DI Diesel Engine (직분식 디젤기관 전자분사계의 광검출기 적용에 관한 연구)

  • Ra, Jin-Hong;Ahn, Soo-Kil
    • Journal of Ocean Engineering and Technology
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    • v.13 no.3B
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    • pp.38-46
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    • 1999
  • Increasing stringent emissions legislation and requirement of more effective energy used for diesel engine demand the fine control of the fuel injection system. Recently, the electromagnetic fuel injection control system for diesel engine is tried to realize the optimum diesel combustion by the feel back sensing as optical signal of combustion flame. The photo detectors were made for the feed back signal of electromagnetic fuel injection control for small DI diesel engine. Their abilities to detect defining combustion events were examined. By evaluating test results, it was shown that the wider acceptable optical range design of optical probe window face, and selection of installation position and installation method of detector were important point for improving sensing ability. The detector was shown to detect start and end of diffused combustion and maximum point of flame intensity impossible for pressure sensor, and also shown that the maximum point of flame intensity was 75% of accumulated heat release point within the experimental conditions.

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