• 한방안이비인후피부과학회지
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• 제18권2호
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• pp.36-50
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• 2005

Background and Objectives: Allergic rhinitis is an allergic reaction characterized by sneezingm itchy nose, mouth and throat, congestion and/or nasal discharge. The offending allergenes are usually pollens, molds, dust mites and animal allergen. Recently, the incidence of infectious nasal diseases tend to decrease. However, allergic rhinitis has increased and treatment in most cases has only deat with the symptom. Tongkyu-tang was composed of sixteen crude drugs. The Oriental Medical References mention therapeutic effects of Tongkyu-tang on nasal obstruction, watery nasal discharg. And Tongkyu-tang has clinically been used for the treatment of common cold, headache, sternutation, rhinitis etc. Speacially Tongkyu-tang is one of the most frequently used medical treatment for the allergic rhinitis. Experimental studies were conducted to investigate for the effect of Tongkyu-tang on the changes of neutrophil segment, lymphocyte, total IgE and nassal tissue in allergic rhinitis of ovalbumin-inhalation rat. Meterial and Methods: Fifteen Sprague-Dawley rats were divided into three group: normal group, control group, experimental group. To induce the allergic rhinitis in control group and experimental group, rats were sentitized intraperitoneally with 0.1 % ovalbumin solution 3 times at intervals of I week. Then intranasal sensitization was performed by diffusing 0.1 % ovalbumin solution 3 times at intervals of 2 days. After that time, rat in the experimental group were oral administration treated by Tongkyu-tang for 28 days. We observed changes in nasal tissue; changes in the number of white blood cell, red blood cell and total Ig E; also changes in the segment of neutrophil and lymphocyte in blood. And we observed the changes of AST, ALT of three group. We used anova test statistically. Result: The number of leucocyte remained unchanged between three group. The number of erythrocyte was increased in experimental group and control group when compared with the normal group. The segment of neutrophil, in blood was decreased in experimental group when compared with the control group but, that was not significant statistically(p<0.05). The promotion of lymphocyte in blood was significantly decreased in experimental group when compared with the Control group(p<0.05). Total IgE was decreased in experimental group when compared with the control group but, that was not significant statistically(p<0.05). The cilium be well preserved in experimental group: the nasal tissue in experimental group was similar to in the normal group. Congestion and expantion of grandular cell in nasal submucosa, hypertropy of epithelium in nasal mucosa, acid mucus in epithelium and neutral mucus in subepithelium were decreased in experimental group when compared with the control group. Effect of Tongkyu-tang on the liver function were also studies in rats. Treatment of Tongkyu-lang did not affected on AST and ALT. Conclusion: Considering the above experimental results, it is suggested that oral administration treatment using Tongkyu-lang, without worry about liver function injury, decreased response on an Animal model with Allergic Rhinitis.

• 한국양식학회지
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• 제6권3호
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• pp.221-233
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• 1993

범가자미, Verasper variegatus에 대한 유전학적 동정을 위하여 세포 크기, DNA함량, 염색체수 및 핵형분석 등의 세포유전학적 조사와 PCR 기법을 이용한 mtDNA 125 ribosomal RNA gene의 분석을 실시하였다. 본 종의 적혈구와 핵의 평균 부피는 각각 $211.10{\mu}m^3$와 $23.03{\mu}m^3$였으며, haploid DNA content는 0.79 pg/cell로서 잉어의 $46.5\%$, 포유류의 $22.6\%$로 나타났다. 염색체 수는 46개로 모두 acrocentric 염색체로 구성되어 있었으며, heteromorphic한 성 염색체는 관찰되지 않았다. PCR 기법을 이용하여 증폭된 범가자미 mtDNA의 12S rRNA gene segment는 대략 390bp로 나타났고, 12S rRNA gene의 PCR product를 제한 효소로 처리 결과, Ava I, Mae II, Sma I, Xba I는 1개의 restriction site가, Mae I는 2개의 restriction site가 관찰되었다. 범가자미 mtDNA의 12S rRNA gene segment의 염기 서열을 인간과 차넬메기와 비교한 결과, identity가 차넬메기 와는 $81.8\%$, 인간과는 $67.7\%$였다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2012년도 춘계학술대회
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• pp.909-912
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• 2012

광학 현미경을 통해 일정한 시간 간격으로 얻은 세포 이미지들로부터 세포의 변화 과정을 관찰하여 어떻게 변화되어 가는지 자동적으로 추적하고 분석하는 것을 자동화된 세포 트래킹이라고 한다. 본 연구에서는 수 천 개 혹은 수 만개의 세포를 하나의 이미지에 포함함으로 크기가 매우 작아진 세포 클러스터를 분리하기 위한 타원 근사 기반의 알고리즘을 제안하고 개발하였다. 제안된 방법은 클러스터의 경계선을 추출하여 라인 세그먼트들로 분리한 다음 휴리스틱을 이용하여 라인 세그먼트들을 결합해 가며 근사 타원을 생성한다. 실험 결과 제안된 알고리즘은 두 개의 세포가 겹쳐진 클러스터의 경우 평균적으로 91%의 정확도로, 세개의 세포가 겹쳐진 클러스터의 경우 평균적으로 84%의 정확도를 가지도 클러스터를 분리해 주었다.

• Journal of Chest Surgery
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• 제47권1호
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• pp.55-58
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• 2014

Mixed squamous cell and glandular papilloma of the lung is an extremely rare benign epithelial tumor showing a mixture of squamous and glandular epithelium. Here, we report a case of mixed squamous cell and glandular papilloma that presented as a solitary nodule in the left lower lobe of a 64-year-old woman. Chest computed tomography demonstrated a lobulated mass in the basal segment of the left lower lobe. The patient underwent a lobectomy under the suspicion of lung malignancy. The histopathological diagnosis was mixed squamous cell and glandular papilloma.

• Applied Microscopy
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• 제25권2호
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• pp.39-52
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• 1995

Pinealocytes in the lower vertebrate are known to have photoreceptive function. These photoreceptor cells have been characterized morphologically in various species of lower vertebrates. No such ultrastructural studies, however, were reported in fresh water turtle. The purpose of this study is to characterize the pinealocytes and the phylogenetic evoluton of these cells is discussed in terms of functional analogy. I. Light microscopy: The pineal body was divided into incomplete lobules by connective tissue septa containing blood vessels, and parenchymal cells were arranged as irregular cords or follicular pattern. In the lobules, glandular lumina were present and contained often densely stained materials. II. Electron microscopy: The pineal parenchyma had three categories of cells: photoreceptor cells, supportive cells and nerve cells. The photoreceptor cells had darker cytoplasm compared to the supportive cells, and the enlarged apical cytoplasm(inner segment) containing abundant mitochondria and dense cored vescles protruded into the glandular lumen in which lamellar membrane stacks(outer segment), dense membranous materials, and cilia were present. Some of these lamellated membrane stacks appeared to be dege-nerating while others were apparently newly formed. Constricted neck portion of the photoreceptor cells contained longitudinally arranged abundant microtubules. centrioles and cross-striated rootlets. Cell body had well developed Golgi apparatus, abundant mitochondria, dense granules($0.5{\sim}1{\mu}m$), dense cored vesicles($70{\sim}100nm$), and rough endoplasmic reticulum occasionally with dense material within its cisterna. Basal portion of the photoreceptor cells had basal processes often with synaptic ribbons, which terminate in the complicated zone of cellular and neuronal processes. Synatpic ribbons often made contact with the nerve processes and the cell processes of neighboring cells. In some instances, these ribbons were noted free within the basal process and were also present at the basal cell mem-brane facing the basal lamina. Obvious nerve endings with clear and dense cored vesicles were observed among the parenchymal cells. Photoreceptor cells of the snapping turtle pineal body were generally similar in fine structure to those of other lower verterbrates reported previously, and suggested to have both photoreceptive and secretory functions which were modulated by pinealofugal and pinealopedal nerves. The supportive cells were characterized by having large dense granules($0.3{\sim}1{\mu}m$), abundant ribosomes, well developed Golgi apparatus and rough endoplasmic reticulum. These cells were furnished with microvilli on the luminal cell surfaces, and often had centrioles, striated rootlets, abundant filaments especially around the nucleus, and scattered microtubules. Some supportive cells had cell body close to the lumen and extended a long process reaching to basal lamina, which appeared to be a glial cell. Nerve cells within the parenchyma were difficult to identify, but some large cells located basally were suspected to be nerve cells, since they had synaptic ribbon contact with photoreceptor cells.

• Journal of Chest Surgery
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• 제27권6호
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• pp.486-488
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• 1994

The mucoepidermoid cancer is a tumor arising in the bronchial submucosal glands that shows an intimate admixture of glandular element and sheets of cell with or no definite squamous differentiation. This rare tumor is usually located in lobe and bronchi and occasionally in the trachea. This tumor presents with symptoms of bronchial irritation or obstruction, often of several years duration. The treatment is complete resection with use of bronchoplastic techniques.Low grade tumor have a good prognosis with adequate resection. We experienced a case of mucoepidermoid cancer arising from superior segment of left lower lobe, which was treated with Lt.lower lobectomy.

• 대한기계학회논문집B
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• 제37권3호
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• pp.259-266
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• 2013

광학 현미경을 통해 일정한 시간 간격으로 얻은 세포 이미지로부터 세포 변화를 자동적으로 추적 및 분석하는 것이 세포 트래킹이라고 한다. 세포 변화 과정에서 이웃에 있는 세포들이 겹쳐져 있는 상태를 클러스터라고 하며 세포트래킹에서 클러스터를 다시 세포로 분리하는 작업은 매우 중요하다. 본 논문에서는 타원 근사법을 기반으로 클러스터를 분리하기 위한 알고리즘을 제안한다. 클러스터의 외곽선을 추출한 후 외곽선의 오목정점을 이용하여 클러스터를 라인 세그먼트들로 분리한 다음 휴리스틱을 이용하여 라인 세그먼트들을 결합해 가며 근사 타원을 생성한다. 실험 결과 두 개의 세포가 겹쳐진 클러스터의 경우 평균적으로 91%, 세 개의 세포가 겹쳐진 경우 평균적으로 84% 그리고 겹쳐진 세포의 개수가 네 개 이상인 경우 약 73%의 정확도로 클러스터를 분리해 주었다.

• 한국정보기술학회 영문논문지
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• 제10권2호
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• pp.199-214
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• 2020

An important technique of the present invention is primarily to parallel light detection, self-pulse therapy after diagnosis. Herpes zoster is a disease caused by varicella zoster virus, and the virus that has been latent in the dorsal root ganglion that controls the skin segment loses its immune system and physically damages it. It is an acute skin disease in which acute pain and bullous rash occur along the sensory ganglia, which are rehab by inducers such as malignant tumors. Dorsal root ganglion after complete recovery of varicella, relapsed after incubation in brain ganglion, latent virus sometimes suppressed activity by cell mediated immunity, and in cell ganglion with reduced cellular immunity. It proliferates and destroys neurons, causing pain while forming a rash and blisters. This can reduce cell necrosis and increase the phagocytosis and enzymatic activity through the movement of ions through the cell membrane, depolarization and membrane potential change, growth factor secretion, calcium ion transfer, chondrocyte synthesis, etc., And may offer treatment options for lesions of herpes zoster and post-herpetic neuralgia (PHN).Therefore, according to the present research, the diagnosis and treatment device of treating paing for herpes zoster and post-herpetic pain can be implemented in the early stage of herpes zoster, and conventional analgesic regulation, anti-inflammatory effect, post-herpetic neuralgia.

• Applied Microscopy
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• 제32권1호
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• pp.17-30
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• 2002

살오징어 (Todarodes pacif icus)와 서해낙지 (Octopus minor)의 망막은 외절부, 간상기저부, 내절부 그리고 망상층 등 4개의 층으로 구분되었고, 망막의 두께는 오징어 ($385{\sim}400{\mu}m$)에서보다 낙지 ($400{\sim}420{\mu}m$)에서 $20{\mu}m$ 정도 더 두터웠다. 망막은 시각세포와 지지세포로 구성되어 있는데, 서해낙지의 지지세포 상단에는 미세융모 (길이 $0.6{\sim}0.7{\mu}m$)가 밀생된데 비해 살오징어에서는 확인되지 않았다. 망막을 구성하는 시각세포와 지지세포들은 광선을 차단하는 색소과립들을 소지하고 있었는데, 살오징어인 경우 시각세포의 색소과립(크기, $2.0{\times}0.5{\mu}m$ 정도)이 지지세포의 색소과립 (크기, $1.0{\times}0.3{\mu}m$ 정도)에 비해 컸으나, 서해낙지에서는 두 세포간 색소과립의 크기 ($0.8{\times}0.6{\mu}m$ 정도)가 비슷하였다. 시각세포의 세포질 상단부에서 빗살모양의 미세융모들이 정육각형 구조 (직경, 60 nm)인 rhabdome을 형성하였는데 한 개의 rhabdome은 4개의 rhabdomere로 구성되어 있으며, 전체 rhabdome의 총 단면적은 서해낙지가 살오징어에 비해 두 배정도 컸다. 망상층을 구성하는 연접체는 살오징어인 경우 전자밀도가 높은 핵을 포함하는 소포 (electron dense-core vesicle)와 전자밀도가 낮은 소포 (electron lucent vesicle)들로만 형성된 연접체 등 두 종류를 소지한 반면, 서해낙지에서는 전자밀도가 높은 소포(electron dense vesicle)와 전자밀도가 낮은 소포들이 혼합된 형과 전자밀도가 낮은 소포들로만 이루어진 연접체 등 두 종류를 소지하고 있어 두 종간 차이가 확인되었다.

• 폴리머
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• 제34권5호
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• pp.442-449
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• 2010

본 연구에서는 hard segment 함량(%)별로 친수성의 폴리우레탄 프리폴리머를 합성한 후 다양한 발포 혼합 조성액을 이용하여 폴리우레탄 폼을 제조하였다. 그 결과 사슬연장제를 도입한 폴리우레탄 프리폴리머가 도입하지 않은 것에 비해 기계적 물성이 우수하였다. 또한 폴리우레탄 구조에 hard segment 함량(%)을 높임으로써 폴리우레탄의 기계적 물성을 향상시킬 수 있었다. 한편 발포 혼합조성액에서도 F-68, 글리세린, CMC 등의 원료 배합비를 조정함으로써 최종적으로 제조된 폴리우레탄 폼의 기계적 물성과 흡수도, 흡수속도, 모폴로지 등을 조절할 수 있었다. 제조된 폴리우레탄 폼은 세포배양 결과 세포적합성이 우수하였고, 동물실험 결과 대조군인 거즈에 비하여 월등한 창상치유 효과를 보였으므로 창상치료용 소재로서의 적용 가능성이 높음을 알 수 있었다.