• Journal of Forest and Environmental Science
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• v.34 no.3
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• pp.199-208
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• 2018

Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.576-581
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• 2008

The germination responses of Fraxinus rhynchophylla seeds collected from four provenances to constant temperature were investigated over the range $5{\sim}35^{\circ}C$. Difference among seeds in percentage and rate of germination and cardinal temperatures was observed. The seeds from Inje had high germination percentage at low temperature ($5{\sim}15^{\circ}C$) whereas those from Gangneung had high germination percentage at high temperature ($30{\sim}35^{\circ}C$). Three cardinal temperatures viz., the base ($T_b$), the maximum ($T_m$) and the optimum ($T_o$) for germination percentage and germination rate varied among four provenances. $T_b$, $T_m$ and $T_o$ for F. rhynchophylla seed germination as estimated by the quadratic models were the lowest in Inje while those were the highest in Gangneung. The cardinal temperatures ($T_b$, $T_m$ and $T_o$) were estimated by linear sub- and supra-optimal models for germination rate as a function of temperature response. $T_b$ was the lowest in Hoengseong while that was the highest in Gangneung. $T_m$ and $T_o$ were the lowest in Inje while those were also the highest in Gangneung. That is, the seeds from the provenance where the annual mean temperature was high had the higher cardinal temperatures ($T_b$, $T_m$ and $T_o$) as compared to seeds from the provenance where the annual mean temperature was low.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.253-258
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• 2015

In South Korea, Pinus pumila (Pall.) Regel (dwarf stone pine) has been designated as a critically endangered species by the Korea Forest Service. We have difficulties in obtaining the seeds of P. pumila because P. pumila grows only in the Daecheongbong area (1550–1700 m above sea level) of Mt. Seorak and almost all of its cones are damaged by birds and rodents. For establishing an ex situ conservation stand of P. pumila, this study was conducted to figure out the effects of wet cold (cold stratification, prechilling) and GA₃ treatment on the germination of P. pumila seeds. After cold stratification (1, 2, 3, 4, 5 months), prechilling (1, 2, 3, 4, 5 months) and GA₃ treatment (0, 100, 500, 1,000, 2,000, 3,000 ㎎/L), seeds were placed on petri-dishes at 25℃ under light condition. The percentage of germination, mean germination time and the germination rate were investigated. The results showed that both of the cold stratification and prechilling were effective in improving germination performances. However, there were no significant differences in performances between the two cold treatments. Within each treatment, the germination performances improved with the period of treatment. However, after three months of treatment, the performances showed no significant improvement. The gibberellin treatment was also effective in improving seed germination of P. pumila. The percentage of germination reached 79.0% in the seeds treated with 100 ㎎/L of GA₃. However, the germination performances decreased at high concentration of GA₃ treatments (over 2000 ㎎/L). In conclusion, cold stratification (over 3 months) or 100 ㎎/L of GA₃ treatment was considered to be the appropriate method for seedling production of P. pumila.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.1
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• pp.1-10
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• 2022

This study was conducted to investigate the germination characteristics through the temperature change treatment compared to the general room temperature immersion treatment of the native species, the Larix kaempferi, and the Berula platyphyllavar. japonica seeds, and to obtain basic data for the production of healthy seedlings with high productivity and efficiency. As a result, the germination rate of the larch seeds showed a significant difference according to room temperature and temperature immersion treatment, and the average germination days of the birch seeds showed a significant difference according to room temperature immersion and temperature immersion treatment. On the other hand, there was no significant difference in germinal uniformity. The results of the study are as follows. First, the results of the analysis of the fallen leaves were the highest germination rate in the treatment of 1.5 days of hot water and 1.5 days of cold water, the fastest germination rate, the lowest average germination day, and the highest germination uniformity. This is the best result in most items, and the change temperature immersion treatment of hot and cold baths was superior to the conventional room temperature immersion treatment. Second, the results of birch tree showed that the germination rate was the highest in 1.5 days of hot water and 1.5 days of cold water. The average germination day is 1.5 days of cold water 1.5 days of hot water 1.5 days, but the difference between 1.5 days of hot water and 1.5 days of cold water is 0.01 days. The highest germinated uniformity was found in 1.5 days of cold water and 1.5 days of hot water. As a result, in the case of larch and birch, the seed temperature immersion (1.5 days of hot water, 1.5 days of cold water) treatment is superior to the existing three-day immersion treatment in various analysis methods, and it is expected that productivity and efficiency can be improved at a low cost in a short period of time at the seedling production site through seeds.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.316-322
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• 2006

This study was conducted to examine the changes of seed characteristics and germination properties and to determine the most suitable collection time in Ulmus davidiana var. japonica. Seeds were collected six times with the interval of seven days after April 21, 2005 at Jeong-seon. There were differences in seed characteristics and germination properties among seed collection times. The sizes of seed and fruit increased with the collection time, but seed moisture contents decreased with the collection time after increasing until May 5. The 1,000 seeds weight decreased with the collection time after increasing until May 12. Collection time showed high positive correlations (r=0.790, p<0.01) with seed size and negative correlations (r=-0.919, p<0.01) with moisture contents. Percent germination (PG) presented the highest value in seeds collected at May 5, and decreased with the collection time. Mean germination time(MGT), germination speed(GS), and germination performance index(GPI) also showed the highest values in seeds collected at May 5. PG, MGT, and GS had correlations with other seed characteristics except the 1,000 seed weight. The relative growth rate of height and root collar diameter of seedlings presented the highest values in seedling germinated from seed collected at May 5, and seedlings germinated from seeds collected at May 5 and 12 showed the highest values in chlorophyll contents. Consequently it will be possible to product a number of seedlings when the seeds of U. davidiana var. japonica are collected just before drying morphologically.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.7-15
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• 2018

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

• Korean Journal of Food Science and Technology
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• v.17 no.6
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• pp.516-521
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• 1985

Changes in phosphorus compounds and phytic acid of mung bean, soybean, sesame and perilla were investigated during soaking in water, germination, incubation at $60^{\circ}C$ and autoclaving at $120^{\circ}C$. The results showed that total P in all the seeds was decreased slightly by soaking and incubation, but increased in mung bean, soybean during germination. Inorganic P was increased for all the seeds after soaking for 24 hrs, while its content in mung bean, soybean and sesame was significantly increased during germination. Phytic acid content in mung bean, soybean and sesame was significantly seduced by 92 %, 76 % and 78 %, respectively, after 5-6 days of germination. Soaking the seeds for 24 hrs resulted in the reduction of phytic acid by the range of 8-25 %. It was also found that incubation at $60^{\circ}C$ for 10 hrs or autoclaving at $120^{\circ}C$ for 4 hrs affected significantly for removal of phytic acid from the legume seeds.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.341-345
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• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.

• Korean Journal of Medicinal Crop Science
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• v.25 no.4
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• pp.209-216
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• 2017

Background: Dehisced ginseng seeds need to be stored at cold temperatures for around 3 months to break their physiological dormancy, and thus, to aid in gemination. In the presence of high moisture in such an environment, seed spoilage and pre-germination may lower seed quality and productivity. To improve seed quality during cold-stratification, the effects of seed dehydration and temperature were tested. Methods and Results: In early December, dehisced ginseng seeds were dehydrated at 4 different levels and stored at $2^{\circ}C$ $-2^{\circ}C$, and $-20^{\circ}C$ for 3 months. Germination was carried out on the filter papers moistened with distilled water; emergence of root, shoot, and seed spoilage were assessed. Seed viability was examined by the tetrazolium test. More than 90% of the seeds stored at $2^{\circ}C$ and $-2^{\circ}C$ without drying or endocarp dehydration germinated, but seeds that were dehydrated to have a moisture content (MC) below 31% showed poor germination and lost their viability. In addition, the seeds stored at $-20^{\circ}C$ failed to show effective germination. Conclusions: Seed storage after endocarp dehydration might help to improve seed quality and increase seedling's ability to stand during the spring-sowing of ginseng.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.322-325
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• 2003

Controlled hydration treatment of tobacco seeds enhanced seed performance greatly without additional materials associated with conventional osmotic or solid matrix priming technique. The seeds were hydrated by adding water to a level from 10 to 60% by 5% increments and incubated for 8 days at $25^{\circ}C$. After the treatment, the seeds were dried to the original seed moisture content under $20^{\circ}C$ for 72 hours. The moisture content of tobacco seeds equivalent to 35% by the hydration treatment gave the greatest improvement in germination rate and speed compared to untreated or polyethylene glycol (PEG) primed seeds, especially at low temperature of $15^{\circ}C$.