• 한국작물학회지
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• 제46권4호
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• pp.321-324
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• 2001

Tobacco seeds (Nicotiana tabacum L. cv KF109) were primed in the polyethylene glycol 6000(PEG) solution and then stored at 5 and $25^{\circ}C$ under 40, 60 and 80% relative humidity (RH) conditions for six months. The effect of storage temperature and humidity on mean germination time ($T_{50}$), longevity and germination of the primed tobacco seeds were compared. Untreated seeds (control) stored at $5^{\circ}C$ showed high germinability throughout the entire storage period and humidity, and a decline in germinability showed after 6 months at 60% RH and after 3 months at 80% RH when stored at $25^{\circ}C$, Primed seeds retained high germinability until 6 months at 60% RH and 3 months at 80% RH when stored at $5^{\circ}C$ but showed a significant decline in germinability after 3 months at 40% RH, and 1 months at 60% and 80% RH, respectively when stored at $25^{\circ}C$, Primed seeds were completely lost viability when stored at $25^{\circ}C$ under 60% RH for 6 months and under 80% RH for 3 months.

• 한국자원식물학회지
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• 제14권3호
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• pp.251-258
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• 2001

한국 자생 매발톱꽃속 종자 식물유전자원의 장기저장을 위해 건조 전처리에 의해 종자 수분함수율을 저하시킨 후 액체질소(-196$^{\circ}C$) 중에서 생존 가능성 및 보존에 관해 시험하였다. 종자 수분함수율은 10분~2시간에 걸친 다양한 건조조건에 의해 9.7~3.2%로 비례적으로 감소하였다. 건조 전처리한 매발톱꽃속 종자를 액체질소 내에 6개월간 저장하여 38$\pm$5$^{\circ}C$ 온수에 해동시켜 발아율 및 발아세, 평균발아기간을 조사하였다. 건조 무처리 한 수분 함수율10.9% 조건의 매발톱꽃 종자는 초저온 보존 후, 발아율이 52.5%로 나타났으나 60분간 건조 전처리 하여 수분 함수율이 6.0%로 조정된 종자의 경우는 84.7%가 발아하였다. 또한 발아세에 있어서도 수분 함수율이 5% 내외로 적절히 건조 전처리 한 매발톱 꽃속 종자는 초저온 보존 이후에 60%이상의 높은 발아세와 11~13일의 고른 평균 발아기간이 나타났다. 본 시험의 결과, 적절한 처리시간에 의해 건조 전처리한 자생 매발톱꽃속 종자를 초저온 조건의 액체질소에 장기 저장하여도 대조구에 비해 발아율은 약 0.5%가 저하하였으나 발아세, 평균발아율에는 큰차이가 나타나지 않았다. 또한 발아묘의 기형적인 형태적 변화도 관찰되지 않았다. 그러므로 단명성인자생 매발톱꽃속 종자의 초저온 보존은 식물 유전자원을 유전적으로 안전하고 경제적으로 저장할 수 있는 방법으로 사료된다.

• 한국작물학회지
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• 제33권1호
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• pp.59-63
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• 1988

인삼종자의 배생장에 미치는 온도 및 내과피의 영향을 구명하는 한편 미숙배의 생장적온과 후숙배의 저온처리 및 발아적온을 밝히기 위하여 본 실험을 수행하였던 바 그 결과를 요약하면 다음과 같다. 1) 1$0^{\circ}C$ 이하에서는 종자 내과피의 유무에 관계없이 배의 생장은 정지되고 휴면상태로 되어 있다. 2) 15$^{\circ}C$ 및 2$0^{\circ}C$에서는 내과피제거＋종자소독 종자의 배생장이 현저히 촉진되었으며 다음이 무처리대조구종자였고 내과피를 소독한 종자의 배생장은 무처리대조구종자에 비해 지연된 결과를 나타내었다. 3) 각 처리종자 모두 2$0^{\circ}C$보다는 15$^{\circ}C$에서 배의 생장속도가 가장 빨랐던 바 고려인삼종자 배의 생장 최적온도는 15$^{\circ}C$ 근처로 인정된다. 4) 배 후숙종자의 저온처리는 5$^{\circ}C$에서 100일간의 처리가 종자발아에 가장 효과적이었다. 5) 저온처리를 필한 배 후숙종자의 발아 적온은 15$^{\circ}C$ 근처였다.

• 한국환경과학회지
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• 제12권12호
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• pp.1303-1308
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• 2003

This experiment was carried out to investigate the effects of heavy metals (cadmium, chromium, copper and lead) on the seed germination of Arabidopsis thaliana, and examinated the removal effects of biostone and green tea on the heavy metal toxicity. Cadmium and chromium among the four heavy metals had no effect on the seed germination even in the concentration fifty times higher than in the official standard concentration of pollutant exhaust notified by the Ministry of Environment. However, seeds were not germinated in the concentration of copper ten times higher and in the concentration of lead fifty times higher than the official standard concentration. When seeds were sown in the solutions of lead (15, 20, 25 and 30 mg/L) and copper(15 and 20 mg/L), the seed germination rates were 0％ and less than 10％, respectively. However, when biostone(3 g/30 $m\ell$) was added, the seed germination rate was 100％ in all the concentrations. The germination rate was 100％ in distilled water and copper solution (5 mg/L). However, green tea (0.2 g/30 $m\ell$) was added, the seed germination rate was 0％ in both. The results show that cadmiun and chromium had no effect on the seed germination, but lead and copper decreased the rate of seed germination of Arabidopsis thaliana, Biostone removed heavy metal toxicity, but green tea did not removed heavy metal toxicity during germination.

• 원예과학기술지
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• 제34권3호
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• pp.363-371
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• 2016

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at $30^{\circ}C$ under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as $GA_3$ and kinetin, as well as minerals such as $KNO_3$ and KCl. An analysis of germination characteristics at $30^{\circ}C$ under light conditions revealed that treatment with 200 and $500mg{\cdot}L^{-1}GA_3$ led to an excellent germination rate of 97.0%. Treatment with $100mg{\cdot}L^{-1}GA_3$ led to a better germination rate than the control as well. However, treatment with kinetin, $KNO_3$, and KCl had no effect on germination, regardless of concentration.

• 원예과학기술지
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• 제32권6호
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• pp.901-905
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• 2014

The efficacy of ozone micro-bubble water (OMBW) in reducing microbial populations on alfalfa seeds was investigated in this study. We observed the surface of alfalfa seeds using microscopy and found that many cracks and crevices existing on the surface could harbor pathogens. Alfalfa seeds were treated with tap water (TW), micro-bubble water (MBW), ozone water (OW), ozone micro-bubble water (OMBW), and chlorine water (CL) for 5 min, and total microbial population, E. coli and Salmonella spp. colonies were determined. Also, the sterilized seeds were germinated and cultivated for 5 d after sowing to investigate the percentage of germination and the growth of alfalfa sprouts. The treatments with OMBW and CL were most effective in reducing total microbial populations and E. coli was eliminated by OW, OMBW, and CL treatments. CL treatment reduced the percentage of germination and fresh weight of alfalfa sprouts, but OMBW did not cause any negative effects on the germination and growth of alfalfa sprouts. These results indicate that OMBW can be used as an effective sanitizer for eliminating seed-borne pathogens without detrimental effects on seed viability.

• 한국자원식물학회지
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• 제36권6호
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• pp.580-587
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• 2023

Androsace septentrionalis is a grass species restricted to North Korea; however, it is at the brink of extinction due to habitat loss and environmental changes caused by natural disasters and anthropogenic activities. This study was conducted to characterize the dormancy conditions of A. septentrionalis in an effort to conserve this North Korean plant resource. For this purpose, the morphological characteristics and vigor of A. septentrionalis seeds were examined, and its germination characteristics under different temperature conditions (15/6℃ and 25/15℃ ), low-temperature stratification, and gibberellin (GA₃) treatment were determined. The results revealed that A. septentrionalis exhibits non-deep type morpho-physiological dormancy, and low-temperature stratification treatment was not effective in breaking the dormancy of A. septentrionalis seeds. Meanwhile, GA₃ treatment significantly increased the mean germination time, rate, and speed of the seeds. The optimal conditions for the germination of A. septentrionalis seeds were 25/15℃ fluctuating temperature and 500 mg·L¹ GA₃ treatment. The results of this study are useful for the mass propagation of A. septentrionalis.

• 한국자원식물학회지
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• 제1권1호
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• pp.88-92
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• 1988

The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.

• 한국약용작물학회지
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• 제11권5호
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• pp.321-328
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• 2003

Various presowing seed treatments have been used to enhance the rates of germination and afterward seedling emergence. Seeds treated by them occasionally have not shown the same rate in indoor and field. The presowing treatments considering germination mechanism and factors affecting germination should be totally included in indoor experiments so that the results drawn could be reproduced in the field. Seed drying after the treatments and field conditions after sowing especially able to show Phytochromemediated responses related to light treatment must be determined prior to force the treatments to seeds. After set up these prerequisites to promote the rates of germination and emergence, many seed treatments proposed so far must be fixed how to be done; alone, sequential and simultaneous.

• Journal of Forest and Environmental Science
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• 제34권3호
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• pp.199-208
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• 2018

Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.