• 한국산림과학회지
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• 제109권1호
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• pp.41-49
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• 2020

본 연구는 벌개미취 종자의 대면적 파종 효율성을 증진시키기 위하여, 적절한 종자 전처리 조건과 펠렛코팅 소재를 선정하고자 실시하였다. 벌개미취 종자는 충실종자를 사용하기 위해 1일간 침지처리 후 침강 종자만을 사용하였다. 종자 전처리는 GA₃(200, 500 ppm), 24-epibrassinolide(10^-6, 10^-7, 10^-8 M) 호르몬 처리와 염류삼투프라이밍(100 mM KNO₃) 처리로 구분하였다. 펠렛코팅은 무처리와 프라이밍 처리 종자에 피복물질(DTCS와 DTK)과 접착제(PVA와 CMC)를 적용하여 제작하였다. 종자들은 필터페이퍼, 상토, 사양토 환경에서 파종하여 파종상에 따른 종자 처리 효과를 비교하였다. 필터페이퍼에 파종한 종자의 발아율은 무처리 종자가 41%, 프라이밍 종자가 65%로 가장 높았으며, 호르몬 처리 간 차이는 없었다. 프라이밍과 DTCS/PVA 펠렛 혼용 종자의 발아율은 71%로 가장 높았으며, 프라이밍과 DTK/CMC 펠렛 혼용 종자의 발아율은 42%로 가장 낮았다. 프라이밍 처리 후 펠렛코팅한 종자는 무처리 펠렛코팅 종자보다 발아율, 평균발아일수, 발아속도가 향상되었다. 온실의 상토에 파종한 프라이밍 종자의 발아율은 58%로 가장 높았으며, 평균발아일수는 9.4일, 발아속도는 7.0%·day로 27% 발아율의 무처리 종자보다 높았다. 또한 DTK/PVA 코팅 종자의 발아율(40%)은 무처리 종자의 발아율(27%) 보다 높았으며, 무처리 펠렛코팅한 종자의 평균발아일수는 15.0-27.0일로 프라이밍 처리 후 펠렛코팅한 종자보다 길었다. 온실의 사양토에 파종한 무처리 펠렛코팅 종자와 프라이밍 처리 후 펠렛코팅한 종자의 발아율은 모두 1-39%의 범위로 무처리 종자보다 낮았다. 펠렛 소재인 DTK는 DTCS보다 높은 발아율을 보였으며, 무처리 펠렛코팅 종자의 평균발아일수는 15.0-19.8일로 프라이밍 후 펠렛코팅한 종자보다 느렸다. 본 연구 결과를 기준으로, 벌개미취 종자의 프라이밍 전 처리는 유묘 출현율에 매우 효과적이며, 프라이밍과 펠렛코팅을 병행할 경우, 펠렛 소재로는 DTK가 더 적합하였고, 접착제는 PVA와 CMC 간 차이가 크지 않기 때문에 모두 사용이 가능한 것으로 판단되었다.

• 한국연초학회지
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• 제4권2호
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• pp.37-39
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• 1982

This experiment was conducted to obtained basical source for gathering seeds in flue- cured (NC 2326), and to investigate some characters on the relation to gathering number of capsules per a plant; amount of gathering seeds, weight per 1000 seeds and germination rate, respectively. As a results; correlation coefficient of number of capsules and amount of gathering seeds was pros(live correlation (r =0.9771**) , Weight per 1000 seeds (0.16g) was good from 39$\pm$2 capsules and amount of gathering seeds was 4.8g. So it is considered that method of gathering seeds wise proved to be a desirable 39: 2 capsules and amount of gathering seeds was 4- 5 gram.

• 한국작물학회지
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• 제62권1호
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• pp.9-15
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• 2017

본 연구는 벼 무논 및 담수직파 시 입모향상을 위해 철분코팅종자와 일반종자에 대한 발아특성 및 담수깊이에 따른 초기 입모영향을 구명한 결과를 요약하면 다음과 같다. 철분코팅종자는 일반종자보다 발아초기에는 발아율이 높았으나 후기에는 일반종자의 발아율과 비슷하였다. 그 결과 철분코팅종자가 일반종자에 비해 발아속도가 빠르고 평균 발아일수가 짧았다. 철분코팅종자를 상온종자창고에 보관시 1년이 지난 후 발아율은 보관당시보다 15.3~21.6% 감소되어 가능한 철분코팅 종자는 1년 이내에 사용하는 것이 바람직하였다. 또한 담수직파조건에서 철분코팅 종자는 일반종자에 비해 깊은 수심에도 출아율이 높고, 뜬묘비율도 낮을 뿐만 아니라 모 충실도가 높아 담수직파 시 철분코팅종자를 사용하는 것이 초기입모를 향상시킬 수 있는 방법이 될 수 있다.

• 한국환경과학회지
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• 제30권1호
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• pp.77-85
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• 2021

This study was carried out to establish optimal conditions for breaking dormancy of Agastache rugosa O. Kuntze seeds. A series of experiments according to seed maturity and treatment with plant growth regulators were performed to improve germination percentage and synchronize germination of the seeds. In addition, it was conducted to test whether the useful effect of seed treatment before sowing leads to healthy seedling and early vigorous growth. The average seed size was 1.85 mm (length) × 0.82 mm(width). The seed size was much smaller than other vegetable seeds. Seeds colorappeared dark brown, the shape of the seeds was oval and the weight of 1,000seeds was 352.8 mg. The optimum germination temperature was 22℃. Light exposure during germination did not affect germination promotion, suggesting that A. rugosa seeds are a kind of dark germinating seeds. Seed dormancy lasted for 40 days after harvesting, and GA3 treatment of dormant seeds could break dormancy. There were significant differences in germination percentage and rate according to the maturity of seeds. The germination percentage of mature seeds was 10 - 18% higher than that of immature seeds, and germination rate was 2 days faster. GA3 treatment during growth regulator treatment improved germinability, but BAP or ethephone treatment did not. The optimal growth regulator concentration of for germination was the combination treatment of 100 mM GA3 ＋ 100 mM BAP.

• 현장농수산연구지
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• 제18권1호
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• pp.93-100
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• 2016

벼 담수 및 무논직파재배에서 잡초성 벼(weedy rice) 및 잡초(weeds)방제를 위하여 철분 코팅볍씨를 이용하여 발아 전 제초제(pre-emerged herbicides)를 처리하여 안전한 제초제 선발 시험결과를 요약하면 다음과 같다. ① 출아일수는 제초제별로는 벤조비사이클론 >옥사디아존 > 티오벤카브 > 뷰타클로르 순으로, 사용종자별로는 철분코팅 밀봉종자(최아)> 최아종자>침종종자 철분코팅 순으로 출아가 늦었다. ② 사용종자 중 무처리와 벤조비사이클론 처리에서 침종종자 철분코팅의 입모율은 각각 63.7%, 75.7%로 최아종자 및 철분코팅 밀봉종자(최아)에 비하여 23.6~45.2%, 잡초성 벼보다는 46~62.5%나 높았다. ③ 뷰타클로르 제초제의 고사율은 최아볍씨와 철분코팅 밀봉종자(최아)에서는 100%의 고사율을 보였지만 벤조비사이클론은 2%의 고사율을 보여 비교적 안전한 것으로 나타났다. ④ 벤조비사이클론 3.5% 유제 처리의 파종후 25일 초장은 9.2~12.9cm로 무처리 11.9~16.3cm에 비하여 약간 짧았지만 정상적인 생육을 하였다.

• 한국작물학회지
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• 제45권2호
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• pp.108-111
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• 2000

Osmoconditioning and hardening effects for the seed germination of normal and naturally aged rice seeds were studied through analyzing the total sugars and $\alpha$-amylase activity. The normal seeds which used to have high germination rate accelerated germination with the osmoconditioning at the suboptimal temperature of 17$^{\circ}C$. On the other hand, the aged seeds did not affect germination rate at $25^{\circ}C$, while increased germination rate and accelerated germination a little at 17$^{\circ}C$, Hardening of aged seeds increased germination rate by 10-15% compared with control seeds at both 17 and $25^{\circ}C$ and accelerated germination. Total sugar content and $\alpha$-amylase activity of normal seeds were higher than did aged seeds. The aged seeds with treatment of osmoconditioning and hardening increased total sugar content and $\alpha$--amylase activity, but hardening was more effective than osmoconditioning. The $\alpha$--amylase activity was positively correlated with the total sugar content and germination rate.

• 한국작물학회지
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• 제54권4호
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• pp.346-350
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• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• 한국작물학회지
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• 제40권4호
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• pp.407-412
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• 1995

비파괴적인 방법으로 활력이 높은 종자를 선별하는 방법을 개발하기 위하여, 참깨, 파, 상추 종자를 상대습도 90%, 온도 45$^{\circ}C$에서 인위적으로 퇴화시켜 활력이 다른 종자를 물에 침지하였을 때 종자가 분비하는 당, 아미노산, 단백질의 양과 속도 등을 조사한 결과를 요약하면 다음과 같다. 1. 참깨 종자는 활력과 침지시간에 관계없이 당, 아미노산, 단백질을 거의 분비하지 않았다. 2. 파의 죽은 종자는 건전종자나 활력이 중인 종자보다 아미노산을 더 많이 분비하였으나 전당과 단백질 분비량은 크지 않았다. 3. 상추의 죽은 종자는 당, 아미노산, 단백질을 모두 많이 분비하였다. 건전종자와 활력이 중인 종자는 당은 거의 분비하지 않았으나 아미노산과 단백질은 다소 많이 분비하였다.

• Journal of Plant Biotechnology
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• 제40권1호
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• pp.43-48
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• 2013

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

• Journal of Forest and Environmental Science
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• 제37권3호
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• pp.235-242
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• 2021

Seed morphology, physiology and environmental conditions have influence on germination of any tropical seeds and their appropriate handling, processing and handling enhances seeds emergent. This study therefore investigated effects of storage durations under different temperatures on germination of Bombax costatum seeds from different provenances. Fresh 25 seeds of B. costatum in four replicates were collected from four different provenances (Aponmu, Oluwa, Ibadan and Oyo). They were surface sterilized, thoroughly washed in distilled water and stored at four different temperature regimes: 28℃, 21℃, 5℃ and -17℃. Samples were taken every 2 months for germination test for 18 months. Germination assessment was carried out daily and recorded. The experimental design was 4×4×10 factorial in 4 replicates. Data were subjected to percentages and analysis of variance (ANOVA). Results showed that there were significant differences among storage temperatures, storage durations and provenances on germination percentage. Freshly collected seeds from Aponmu and Oyo had the highest mean germination of 100% followed by seeds from Ibadan (89.3%). Seeds from Oyo provenance stored 5℃ had germination of 94.0%, 88.70% and 78.7% at 2nd, 4th and 6th month respectively. Seeds from Ibadan and Oyo stored at 28℃, 21℃ recorded 0.0% starting from 8 months of storage. Germination of B. costatum seeds from the four provenances decreased with increase in storage duration at different temperatures.