• ALGAE
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• v.37 no.2
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.753-760
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• 2021

This study was carried out to discover suitable packaging methods during the cold or frozen storage of sawtooth oak and Japanese larch seedlings, which are major afforestation tree species in Korea. The weight loss rate of sawtooth oak was lower under frozen storage at -2℃ (maximum, 33.7%) than under cold storage at 2℃ (maximum, 61.0%). A film liner treatment inside the packaging box effectively suppressed the increase in the weight loss rate. For the sawtooth oak containerized seedlings, when stored at -2℃, the rate of weight loss was 17.9 to 25.4% in the untreated group and less than 1% in the film liner treatment group. Similar results were observed with Japanese larch. After two months of freezing at -2℃, both species maintained a low index of 1.0 - 1.1 with regard to the degree of shoot desiccation of containerized seedlings, and there was no significant difference between the treatments. On the other hand, the chlorine dioxide treatment could more effectively reduce the occurrence of mold and shoot desiccation than a PE single treatment in bare-root seedlings. According to these results, it was concluded that frozen storage is generally suitable as a means of long-term storage compared to cold storage of sawtooth oak and Japanese larch. A chlorine dioxide treatment at 20 ppm was effective for maintaining seedling quality levels during storage, demonstrating the good effect of suppressing the occurrence of shoot desiccation and the occurrence of mold.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.150-150
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.158-158
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• 2003

• Journal of Bio-Environment Control
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• v.30 no.1
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• pp.10-18
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• 2021

Acclimatization after grafting and graft-take that in order to produce plug seedlings of high-quality are important plug seedling stage which not reduce the plug seedlings quality before shipment. Appropriate acclimatization environment can not only increase seedling quality before secondary growth period, but also effective in promoting the growth of plug seedlings. This study was conducted to determine the optimal the shading period and shading level for acclimatization of tomato and pepper grafted plug seedlings after graft-take. The tomato and pepper seedlings were used in this experiment, and after graft-take, a tunnel was installed on a bed in glasshouse of venlo type to started shading treatment. The shading levels were 35, 55, 75 and 95%, and the shading periods were 1 and 2 weeks, and non-treatment was set as the control. In the case of tomato grafted plug seedling, plant height, stem diameter, dry weight of root, leaf area were significantly higher at the shading period of 1 week and the shading level was 55%. In the case of pepper grafted plug seedling, plant height, stem diameter, and leaf area were the highest in the shading period of 2 weeks and the shading level was 35%. However, dry weight of root, compactness, and T/R ratio, which seedling quality indicators, were lower than in the shading period of 1 week and the shading level 55%. Therefore, considering the quality of seedlings, it is suggested that shading period of 1 week with shading level of 55% treatment can be suitable to produce high quality grafted plug seedlings of tomato and pepper.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.55-56
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• 1999

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.52-57
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• 2006

This research was carried out to determine the effect of controlling nitrogen application on tiller development, yield, and quality of rice under scanty or excess seedling stands in direct-seeded rice on flooded soil surface. Seedling stand was set to 3 levels: scanty $(60/m^2)$, optimum $(120/m^2)$, and excess $(200/m^2)$. In the scanty plot, additional 4 kg/10a nitrogen was applied at the 3rd leaf stage to promote tiller development. On the other hand, applying 3.3 kg/10a nitrogen at 5th leaf stage in the excess plot was omitted to suppress tillering. Maximum numbers of tillers per plant were 15.2 and 8.6 in scanty and excess plots, respectively, as compared with 9.8 in optimum plot. Productive tiller rate decreased with the increase in number of seedling stands. Regardless of seedling density, the first tiller developed on the 3rd node of rice stem from the bottom. The primary tillers developed at 3, 4, 5, 6, 7 nodes in scanty plot, 3, 4, 5 nodes in optimum plot, and 3, 4 nodes in excess plot. The secondary tillers developed only in some portion of plants in scanty and optimum plots. The order of tiller emergence was negatively correlated to stem length, panicle length, non-productive tiller number, grain number per panicle, and fertility in scanty plot, and to perfect grain ratio in excess plot. In the optimum plot, however, the order of tiller emergence was not correlated to any of the mentioned characteristics. The perfect grain ratio of scanty plot was the highest because green-kerneled rice was a very small portion in the primary tillers as compared with those of optimum and excess plots. Yield indexes of scanty and excess plots were 99%, and 97%, respectively, of the yield (494 kg/10a) in optimum plot. In conclusion, when seedling stands are not at optimum level, rice yield and quality similar to optimum planting density level can be obtained by means of controlling nitrogen application.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.3
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• pp.226-231
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• 1994

This experiment was conducted to evaluate the effect of coated urea complex fertilizer(CUC) on the growth and quality of rice in high-ridged dry seeding and infant -seedling machine transplanting on clay loam and sandy loam in Milyang, Korea, 1993. The CUC level applicated was 100 %, 80 %, 60% and 40% to standard application amount of fertilizer. Results obtained were as follows : 1. The nitrogen releasing rate in dry seeding was 83% for sandy loam, 81% in clay loam for 3.5 months after initial releasing, and in infant-seedling was 89% in both soil types for 4 months. 2. The degree of rice leaf color was the highest at the heading stage, and was high with increasing CUC application level regardless of cultural methods, especially was higher at harvesting stage in the 100% level of CUC to standard application amount of fertilizer than conventional fertilizer. 3. The perfect rice grain ratio was higher in infant-seedling than in dry seeding, but lowered with increasing CUC application level regardless of cultural methods. The green kerneled rice ratio among imperfect rice grain was high in dry seeding, and the notched belly rice kernel ratio was high in infant-than infant-seedling of the both soil types, and increased with increasing CUC application level. 4. Hon-value in rice grain was higher at the treatment of CUC application than conventional fertilizer in dry seeding. However, it was contrary result in infant seedling, and was low tendency with decreasing CUC application. On the other hand, the protein in rice grain was also same tendency of Hon-value. Cel-consistency related to eating quality was longer with increasing CUC application level regardless of cultural methods. However, there was no clear tendency at clay loam. 5. No significant difference between rice yield and CUC application in the range of 60% to 100% at both soil types in dry seeding was observed. These results appeared in clay loam under infant seedling except sandy loam. Accordingly, it was thought that 60% level of CUC to standard application amount of fertilizer could be applicated for growth and quality of rice.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.662-668
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• 2016

No-tillage dry-seeding of rice can offer potential benefits by reducing time and labor cost compared with conventional tillage practices. This study was conducted to investigate the effects of no-tillage dry-seeding on rice growth and soil hardness in comparison with other rice cultivation methods, machine transplanting and wet-hill-seeding on puddled paddy. The seedling stand fell within optimum range for both no-till dry-seeding and wet-hill-seeding on puddled paddy. Plant height, number of tillers and SPAD values in no-tillage dry-seeding cultivation were higher than those observed in other methods. There were no significant differences in grain yield of rice among three cultivation methods. The quality characteristics of milled rice grown in no-tillage dry-seeding were similar to those grown in other cultivation methods. Soil hardness in top 10 cm depth was significantly higher in no-tillage dry-seeding than other cultivation methods, while soil hardness below 10 cm depth was highest in machine transplanting cultivation. Results indicate that no-tillage dry-seeding practice is comparable to conventional tillage system in terms of seedling establishment, growth, yield and grain quality.

• Journal of Forest and Environmental Science
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• v.33 no.3
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• pp.226-232
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• 2017

Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.