• 한국작물학회지
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• 제42권3호
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• pp.333-337
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• 1997

땅콩의 육종년한을 단축하기 위한 세대촉진 방법을 정립하고자 포장후건에서 신대광땅콩 및 남대땅콩을 재배하고, 자방병이 지하로 침투한 후부터 80일까지 10일 간격으로 꼬투리와 종실을 재취하여 그 크기와 무게, 수분함량의 변화를 추적하는 동시에 종실의 발아율과 발아한 유묘의 활력을 조사한 결과, 자방병이 지하로 침투한 후 30∼40일에 꼬투리와 종실의 크기가 완성되었고, 이 때에 수확한 종실은 100％ 발아력을 갖추며 발아 후 활력있는 유묘를 생산할 수 있는 것으로 나타났다.

• 한국자원식물학회지
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• 제19권4호
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• pp.480-487
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• 2006

본 연구는 발아율이 낮고 발아가 불균일할 뿐만 아니라 발아소요기간이 긴 고로쇠나무 종자의 발아율 및 발아속도를 향상시키고자 PEG를 이용한 priming 처리를 실시하였던 바 그 결과를 요약하면 다음과 같다. 수분퍼텐셜을 0, -0.25, -0.5, -1.0, -2.0MPa로 각각 조절한 PEG 용액에 3일 동안 침지시켜 priming 처리한 고로쇠나무 종자는 무처리구에 비해 priming 처리구에서 발아율, 평균발아일수, 발아속도 및 발아균일지수가 향상되었다. 특히, -2.0MPa 처리구에서는 가장 높은 효과를 나타내었다. 또한 priming 처리는 무처리구에 비해 휴면율이 낮게 나타나 휴면타파에 효과가 있음을 알 수 있었다. Priming 처리가 종자발아 뿐만 아니라 유묘활력에도 영향을 미치는지 알아보고자 priming 처리 후 발아된 종자로부터 생장한 유묘를 대상으로 상대생장율 및 T/R율을 조사한 결과 -2.0MPa 처리구의 유묘에서 상대생장율은 가장 높고 T/R율은 가장 낮은 것으로 관찰되었다. 이러한 결과로부터 -2.0MPa의 수분퍼텐셜에서 3일 동안 priming 처리하는 것이 고로쇠나무 종자에 있어 가장 합리적인 종자처리 방법이라 할 수 있었다.

• 원예과학기술지
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• 제33권6호
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• pp.932-940
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• 2015

One of the main factors restricting production of triploid seedless watermelon is poor germination due to weak embryos, thick seed coats, and larger air spaces. This study was carried out to investigate the priming effects of different concentrations of chemicals, including hydrogen peroxide ($H_2O_2$), fusicoccin, and gibberellic acid (GA) on germination and seedling uniformity of triploid watermelon (Citrullus lanatus). Three commercial triploid cultivars, Seedless Plus, Sinus, and Sizero, were pretreated with water and different levels of $H_2O_2$ (2 and 4%), fusicoccin (FC: 1, 5, and $10{\mu}M$), and GA (1, 5, and $10{\mu}M$). The present findings helped to find optimal priming conditions for improving germination of triploid watermelon. Treatment with $5{\mu}M$ GA and hydropriming helped to break seed dormancy, enhancing the final germination percentages in all triploid cultivars and increasing the germination index in Sizero. These seed-priming treatments could be used on large scale for industrial applications. Moreover, hydropriming provides a simple, effective, and costless method to improve seed germination and seedling vigor of Sinus and Sizero varieties.

• 한국작물학회지
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• 제49권5호
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• pp.381-388
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• 2004

In Korea, production of super sweet corn has been economically feasible and is substituting for traditional sweet corn due to better flavor in recent years. Major limiting factors for super sweet corn production are low field emergence and low seedling vigor. The optimum water potential (WP) for the priming of normal and aged seeds of dent, sweet (su) and super sweet (sh2) corns was studied to improve low seed quality. Seeds were primed at 0, -0.3, -0.6, -0.9, and -1.2 MPa of polyethylene glycol (PEG) 8000 solution at $15^{\circ}C$ for 2 days. Priming effects differed depending on the type of corn, seed quality, and WP of PEG solution. Although WP of priming solution did not influence the emergence rate of extremely high quality normal dent corn seeds, it reduced time to $50\%$ emergence (T50) and increased plumule weight. In contrast, the emergence rate of aged field corn was improved by seed priming at 0 MPa and plumule weight and $\alpha-amylase$ activity was enhanced. The optimum WP for both normal and aged sweet and super sweet corn seeds was between -0.3 and -0.6 Mpa. At the optimum WP emergence rate, $\alpha-amylase$ activity, and content of DNA and soluble protein increased, while T50 and leakage of total sugars and electrolytes reduced.

• Weed & Turfgrass Science
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• 제2권3호
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• pp.274-282
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• 2013

본 실험은 ISTA 변온 및 자연실온 환경에서 퍼레니얼 라이그래스 신품종의 종자 발아력, 초기발아속도 및 발아피크기간 등의 특성을 연구 비교해서 실무적으로 한지형 잔디의 설계, 시공 및 관리에 활용하고자 시작하였다. 실험 I은 ISTA 변온(8시간: 광조건, $25^{\circ}C$; 16시간: 암조건, $15^{\circ}C$), 실험 II는 $5-25^{\circ}C$로 유지되고 있는 실내 실온환경에서 수행하였다. 퍼레니얼 라이그래스 종자의 발아력, 초기발아속도 및 발아피크기간은 발아환경 및 품종에 따라 유의한 차이가 나타났다. 종자 발아력은 ISTA 변온환경에서 66.00-93.75%, 자연실온 환경에서 66.25-93.50%로 나타났다. 초기발아속도는 ISTA 변온에서 'Pennant II', 'Brightstar II', 'Sonata', 'Accent II' 품종, 자연실온에서는 'Pennant II' 및 'Brightstar II' 품종이 우수하였다. 품종별 발아피크 기간은 ISTA 변온 환경에서 1.48-5.65일, 자연실온에서는 2.17-10.63일로 나타났다. 발아환경에 관계없이 'Sonata', 'Pennant II' 및 'Brightstar II' 품종의 발아피크 기간이 짧았고, 발아피크 기간이 가장 긴 품종은 'Revenge GLX' 이었다. 본 실험 결과 종자 발아력, 초기발아속도 및 발아피크기간을 종합적으로 고려 시 ISTA 변온에서는 'Pennant II', 'Brightstar II', 'Sonata' 및 'Accent II' 품종이 우수하였다. 그리고 자연실온 환경에서는 'Pennant II' 및 'Brightstar II' 품종이 우수한 것으로 판단되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.

• 한국육종학회지
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• 제42권4호
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• pp.374-380
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• 2010

딸기의 우수한 교배친을 선발하기 위해 11품종의 자방친을 자식시켜 후대실생 개체들의 성능 검정한 결과 초세, 내병성, 수량성 등이 우수한 '아키히메', '매향', '설향' 등 3품종을 자방친으로 선발하였다. 선발된 3품종에 대하여 조합능력 검정을 한 결과 초세는 '아키히메'가 자방친인 조합에서 가장 우수하였고, 성숙기는 '설향'이 자방친인 조합에서 빠른 경향이었다. 그리고 '설향' ${\times}$ '도치오토메', '설향' ${\times}$ '금향', '설향' ${\times}$ '원교3111호', '아키히메' ${\times}$ '원교3111호'등의 조합은 18 g 이상의 대과종의 개체 비율이 높았다, 당도는 조합별로 큰 차이가 없었지만, 경도는 '매향'조합에서 높은 경향이었는데, 특히 '매향' ${\times}$ '도치오토메' 조합에서 높았다. 수량은 '아키히메' ${\times}$ '원교 3111호' 조합에서 가장 많았고, 그 다음이 '설향' ${\times}$ '금향', '매향' ${\times}$ '금향', '아키히메' ${\times}$ '도치오토메' 순으로 높았다.

• 한국작물학회지
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• 제49권5호
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• pp.373-380
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• 2004

The production of sweet (su) and super sweet corns (sh2) has been economically feasible in Korea in recent years. Major factors limiting super sweet corn production are low germination and low seedling vigor. Since seed quality is closely related to seed maturity, the optimum harvest time for the seed production of sweet and super sweet corns was studied and the quality of seeds with varying maturities was investigated in 2001 and 2002 cropping seasons. The parents of the sweet corn seeds were Hybrid Early Sunglow and 'Golden Cross Bantam 70' and those of super sweet corn were Xtrasweet 82 and 'For­tune'. Seeds were harvested at 21, 28, 35, 42, 49, and 56 days after silking (DAS). As the seeds developed, seed weight of sweet corn increased and the seed moisture content decreased faster than that of super sweet corn. Germination rates of sweet corn seeds harvested 21 and 28 DAS at $25^{\circ}C$ and emergence rates in the cold soil test were significantly lower than those of seeds harvested after 42 DAS in both years. Although the germination rates of super sweet corn seeds with varying maturities showed similar patterns as sweet corn seeds at $25^{\circ}C$, the emergence rate of super sweet corn seeds in cold soil test continuously increased with seed maturity. This suggests that seed quality of super sweet corn should be tested in a cold soil test to estimate field emergence. As the seeds developed, leakage of total sugars and electrolytes from the both sweet and super sweet corn seeds decreased up to 42 or 49 DAS. The $\alpha-amylase$ activities of both sweet and super sweet corn seeds increased with seed maturity from 21 to 35 or 49 DAS depending on genotype and year. The optimum harvest time for the seed production of sweet corn was 42 DAS and 49 DAS for super sweet corn considering emergence rate and plumule dry weight in the cold soil test, leakage of sugars and electrolytes from the seeds, and $\alpha-amylase$ activity.

• 한국산림과학회지
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• 제99권2호
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• pp.244-250
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• 2010

The aim of this study was to investigate relationship among seed viability and enzymes activities involved in scavenging reactive oxygen species (ROS), especially, superoxide dismutase (SOD), glutathione reductase (GR), and catalase (CAT). In other respects, osmopriming has been demonstrated to reinvigorate aged seeds. Various viabilities of seeds that were ranged from 80 to 100% of germination rate could be produced using osmopriming and accelerated aging treatments. Priming treatment of Pinus thunbergii seeds for 3 days at $15^{\circ}C$ with a polyethylene glycol solution at -1.2 MPa improved their subsequent germination at $25^{\circ}C$. Accelerated aging (3, 6, 9, and 12 days at $41^{\circ}C$ and 100% relative humidity) decreased seed germination percentage depending on aging treatment duration. Electrolyte conductivities of seeds were measured as assay of membrane integrity. The conductivity from electrolyte leakage of P. thunbergii seed was also correlated with seed germinability. Conductivity for control seeds that had 95% of germination percentage was 3.48 ${\mu}S\;g^{-1}$, but jumped as doubled (7.98 ${\mu}S\;g^{-1}$) in 12-day-aged seed that had 80% of germination percentage. Our results demonstrate that aging of P. thunbergii seeds is associated with changes in the electrolyte leakage, lipid peroxidation, and antioxidant defense system. Priming of aged seeds progressively restored the initial germinative ability and resulted in a marked decrease in the levels of MDA and conductivity of seed leachate. These effects of priming were also well recovered of GR and CAT activities in aged seed. The improved seed quality by priming treatment appears at least partly attributable to reduced lipid peroxidation, resulting from enhanced antioxidative enzyme activities that are suggesting the antioxidant defense systems play a key role in seed vigor.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.186-186
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• 2017

Relay intercropping system of direct seeded rice and winter cereal is labor saving cultivation and has high land use efficiency in Japan. In this system, rice seeds are direct seeded into inter-row space of winter cereals (wheat or barley) in March or April. If the direct seeding of rice is conducted before stem elongation phase or using suitable seeder, these are little effect to yield of winter cereals. Though the seeds are generally thiuram treated, it's a matter that seedling establishment rate (SER) of direct seeded rice is low and unstable. The cause of low SER has not been revealed. In present study, with the aim to reveal causes of low SER, we conducted experiments and investigated the SER, and analyzed some factors that might affect SER. Experiment1: In 2015, 2016, we buried rice seeds underground, and investigated the transition of the seed survival rates (SSR). Seeds were thiuram-treated or non-treated. In 2 periods, SSR of thiuram-treated seeds were significantly higher than non-treated seeds. In 2016, thiuram-treated seeds were high in SSR (almost 75%) at April 30th, but low SER (10~27%) after harvest of winter cereals. Therefore, almost all of seed death might have been happen after germination. Analysis 1: We investigated the SER and cultivation conditions in Ibaraki pref. for several years. Meteorological factors were referred from the nearest point of AMeDAS. From mean temperature (MT) among 5days after and before the day, we divided the period of seeding ~June 20 to phase1~4. We defined each phase as below; Phase1: $MT{\leq}10^{\circ}C$, Phase2: $10^{\circ}C<MT{\leq}15^{\circ}C$, Phase3:$15^{\circ}C<MT{\leq}20^{\circ}C$, Phase4: $MT>20^{\circ}C$. We analyzed the correlation of SER and meteorological factors by each phase. Total number of days in phase 1~4 was significantly negative correlated with SER. In phase1, total rain fall and number of soil wetting days were significantly negative correlated with SER. In phase2~4, only MT was significantly positive correlated with SER. This result suggested that rainfalls in phase1 declined seed vigor to emergence from soil surface, by repeated water absorption and re-dry. From these present studies, it was suggested that one of factors of low SER of direct seeded rice in relay intercropping system is changing of water condition by rainfalls in phase1 ($MT{\leq}10^{\circ}C$). To improve SER, it's necessary to consider something seed treatments such as prevent water absorption during phase1. However, 58~60% of seeds seemed to die during May. It's suggested that, if seeds are thiuram treated, almost all of seeds can germinate underground, but the seed vigor to emergence from soil surface are insufficient. Further studies are needed to reveal the rest causes that is happening during emergence from soil surface.