• 화훼연구
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• 제19권3호
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• pp.181-186
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• 2011

농촌진흥청 원예특작과학원에서는 2003년에 황색 폼폰 화형인 'Restone'에 자주색 폼폰 화형인 'Lollipop'을 교배하여 획득한 실생 집단으로부터 황색 적심 폼폰 화형의 겹꽃 '03B1-82' 계통을 선발하였다. '03B1-82' 계통은 2005년 겨울부터 2007년 3년에 걸쳐 1, 2차 특성검정을 통해 안정성, 균일성 및 흰녹병저항성 검정 및 절화수명에 대해 조사되었고, 2008년에는 우수선발계통으로 '원교B1-142호' 계통번호를 부여받아 3차 특성검정을 실시하여 안정성, 균일성에 대한 연차별 재현성 그리고 주년생산성(촉성, 억제재배), 생산자 및 소비자 기호성을 평가 받았다. '원교B1-142호'는 2008년에 직무육성품종심의회를 거쳐 '옐로우캔디'로 명명되어졌다. 국화 '옐로우캔디'는 10월 하순에서 11월 상순에 자연 개화하는 절화용 스프레이 추국이다. 황색의 적색화심을 지닌 폼폰화형의 겹꽃으로 초세 및 줄기가 강건하다. 특히 여름철 고온기에도 통상화가 많이 발생하지 않으며, 촉성 및 억제재배 등 주년생산이 가능하다. 꽃의 직경이 4.3 cm 내외, 꽃잎수는 소화당 180매 이상, 착화수는 본당 8화 내외의 중형화로 절화수명은 18일 정도이다. 재배상 유의점으로는 둥근모양의 폼폰형이기 때문에 수확시 꽃이 서로 엉키면 꽃이 떨어지는 경우가 있으며, 여름 장마철 또는 겨울철 환기 부족시 흰녹병 발생을 주의하고 주기적인 방제가 필요하다.

• 한국환경복원기술학회지
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• 제3권2호
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• pp.24-34
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• 2000

The project for rehabilitation and revegetation of the abandoned coal-mine lands is a very important national environmental restoration project in the view point of rehabilitation and revegetation of denuded forest-lands caused by coal mining as well as restoration of disturbed natural environment and control of the variable pollutions. In Korea, because a large number of coal mines had been developed in order to fill up abundantly consumption of coal as a major energy source in the developing period, a lot of denuded forest-lands caused by coal mining had distributed in the whole country. And, due to the absence of effective rehabilitation and revegetation works on the denuded forestlands caused by coal-mining, most of them had been remained with being damaged. In 1990, area of the abandoned coal-mine lands, requiring the rehabilitation and revegetation works, was about 1,437.1 ha. For the past ten years ('90~'99), about 1,081.8 ha out of them had been rehabilitated and revegetated, and the rehabilitation planning area was about 33.0 ha in 2000. So, remaining area out of abandoned coal-mine lands will be about 322.3 ha after 2000. In principle, after abandoning coal-mine, mine owners must carry out the rehabilitation and revegetation works on the abandoned mine lands by themselves. But, most of mine owners were in financial difficulty after abandoning coal-mine, so that principle couldn't have obtained the desired effects. To solve this problem, from 1995, Coal Industry Promotion Board (CIPB) have carried out the rehabilitation and revegetation works on the abandoned coal-mine lands at government budgets, and they have obtained good results in the construction area. However, due to application of the "conventional erosion control measures and techniques" to the rehabilitation and revegetation measures on the abandoned coal-mine lands, the results and effects of the works excuted have not been successful. Therefore, unique measures and techniques for rehabilitation and revegetation of the abandoned coal-mine lands will have to be developed, especially including development of new techniques on the soil-dressing and soil-covering, seed spray and hydro-seeding measures with seed-fertilizer-soil materials as the mechanized measures, and using of new materials for the tree planting and seedling measures.

• 한국잡초학회지
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• 제13권1호
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• pp.7-13
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• 1993

농산물(農産物) 수입개방화(輪入開放化)에 대처(對處)하여 농가(農家) 고소득(高所得) 작물중(作物中) 하나인 참깨의 수확(收穫)시 기계화(機械化) 작업(作業)에 의한 노동(勞動)의 효율(效率)과 대량생산(大量生産)에 따른 생산비(生産費) 절감(節減)을 위한 방법(方法)으로 비선택성(非選擇性) 제초제(除草劑)인 paraquat와 diquat를 예취(刈取) 3일전(日前)과 3시간전(時間前)에 각각(各各) 처리(處理)하여 참깨 수확(收穫)을 위한 건조제(乾燥劑)로서의 이용가능성(利用可能性)을 연구(硏究)할 목적(目的)으로 수행(遂行)한 실험결과(實驗結果)는 다음과 같다. 1. Paraquat와 diquat를 예취(刈取) 3일전(日前)과 3시간전(時間前)에 각각(各各) 처리(處理)한 결과(結果) 참깨 꼬투리의 수분함량(水分含量)은 무처리구(無處理區)에 비(比)해 빠르게 감소(減少)되어 건조효과(乾燦效果)가 뚜렷하였다. 2. 공시약제(供試藥劑)를 처리(處理)한 후(後) 수확(收穫)한 참깨의 수량(收量)과 참깨 종자(種子)의 발아율(發芽率)은 무처리구(無處理區)에 비(比)하여 유의차(有意差)가 없었다. 3. 참깨 종자중(種子中)의 약제(藥劑) 잔유량(殘留量)은 두 공시약제(供試藥劑) 모두 예취(刈取) 3일전(日前) 처리농도(處理濃度) 250ppm에서 검출(檢出)되지 않았다. 4. 예취후(刈取後) 일수(日數)에 따른 참깨꼬투리의 탈립율(脫粒率)은 건조제(乾燥劑) 처리(處理)로 무처리(無處理) 실행(慣行)에 비(比)하여 매우 높았다. 5. 탈곡회수(脫穀回數)에 따른 소요노동력(所要勞動力)도 크게 감소(減少)되었다.

• The Plant Pathology Journal
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• 제24권4호
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• pp.453-460
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• 2008

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, $6.5{\times}10^6$ and $2.5{\times}10^7$ spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duck-weed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of $12.5{\mu}g$ per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broad-spectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity ($a_w$) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

• 한국약용작물학회지
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• 제20권5호
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• pp.315-319
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• 2012

This study was conducted to select plant growth regulators effective at ginseng berry set inhibition to help root growth in Korean ginseng (Panax ginseng C. A. Meyer). PGRs (ethephon, gibberellic acid, maleic hydrazide, coumarin) were applied to field grown 5-year-old Korean ginseng between one and two times, before and during bloom in 2009, 2010. The number of treatment was more effective in ginseng berry set inhibition when used two times compared with one time in GA 1,000 ppm, MH (5,000, 10,000 ppm), coumarin (5,000, 10,000 ppm) treatment. According to treatment period of plant growth regulator, ginseng berry set inhibition rate from 20days before flowering date to 5days after blooming was the highest in MH 5000 ppm showing 99.9% and the lowest in GA 100 ppm showing 32.8%. The spray treatments of Ethephon (50, 150 ppm) and MH (5,000, 10,000 ppm) from 20 days before the flowering bloom up to 5 days before, and coumarin (5,000, 10,000 ppm) from 20 days to 6 days and before blooming that induced the inhibitory effect more than 90% after 12 weeks. Considering ginseng berry set inhibition characteristics and treatment period ethephon and coumarin was important about applied period but, MH treatment appeared to effective ginseng berry set inhibition regardless of treatment period.

• 한국유기농업학회지
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• 제21권3호
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• pp.363-372
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• 2013

가지 담배가루이 친환경 방제기술을 개발하고자, 난황유 등 8종의 천연물질을 가지 정식 후 7일 간격으로 살포하면서 생육을 조사한 결과 님제처리에서 가장 양호한 경향이었다. 담배가루이 성충과 약충의 발생은 디노테퓨란약제처리에서 가장 적었고, 친환경자재 중에서는 님제 처리에서 가장 적었다. 수량은 디노테퓨란 약제처리에서 가장 많았고, 친환경 자재에서는 님제, 고추씨유 등이 많았으며, 고삼제 은행과육 등에서 적었다. 님제 처리의 경우 무처리에 비하여 69% 많았고, 디노테퓨란약제에 비해 52% 낮은 수량을 나타내었다.

• 한국환경농학회지
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• 제30권3호
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• pp.323-329
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• 2011

BACKGROUND: The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s): High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.

• 한국방재학회 논문집
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• 제9권1호
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• pp.99-105
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• 2009

본 연구에서는 2006년 집중호우로 인해 발생한 토석류 관련 수해 및 그에 대한 복구현황에 대한 분석을 실시하였다. 이를 위해 문헌조사 및 현장답사를 통해서 2006년에 발생한 토석류의 특성, 이로 인한 수해 및 복구현황 등을 분석하였다. 2006년 강우기간 동안 최대시우량 113.5 mm, 1일 최대 강우량 355 mm의 호우가 발생하였는데 이는 80-500년의 강우빈도에 해당된다. 분석 결과에 의하면 수해의 대부분은 사면유실로부터 기인한 토석류에 의해서 발생하였다. 수해기간 동안의 강수량 자료에 대한 분석결과 강우강도와 수해 발생시점은 직접적인 관계가 있는 것으로 나타났다. 현재 수해와 관련되어 계곡부에 사방댐 및 자연 사면에 대해 사면보호공이 실시되어 있다. 본 연구에서 분석된 결과를 통하여 현행 사면수해 복구 및 관리와 관련된 문제점에 대해 대안을 제시하고자 한다.

• The Plant Pathology Journal
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• 제25권3호
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• pp.280-285
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• 2009

Powdery and downy mildews caused by Sphaerotheca fusca and Pseudoperonospora cubensis are the most common and serious diseases of cucumber worldwide. In spite of the introduction of highly effective systemic fungicides, control of these diseases remains elusive. Hence, this study aimed to develop an alternative method to chemicals in controlling the diseases by using different types of cooking oil. Egg yolk, which contains a natural emulsifier, lecithin, was selected as a surfactant to emulsify the oils. Among the different cooking oils used, soybean, canola (rape seed), safflower, sunflower, olive, and corn oils showed over 95% control values against powdery mildew of cucumber in a greenhouse test. In particular, 0.3% canola oil emulsified with 0.08% yolk (1 yolk and 60 ml canola in 20 l spray) was found to be the most effective. The treatment resulted in 98.9% and 96.3% control efficacies on powdery and downy mildews, respectively, of cucumber in the field. Canola oil exhibited direct and systemic effect, wherein powdery mildew of cucumber was suppressed only on treated leaves but not on non-treated leaves in a plant, while mycelia and conidia of the pathogen were severely distorted or destroyed by the treatment. The prospect of using the canola oil and yolk mixture as a natural fungicide is highly promising because of its effectiveness, availability, low cost, simple preparation, and safety to humans and the environment. The use of the canola oil and yolk mixture is expected to be an effective fungicide for use in organic farming and home gardening.

• Applied Biological Chemistry
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• 제45권4호
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• pp.185-189
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• 2002

우리나라의 유전자변형농산물 의무 표시제가 시행됨에 따라 수입 유전자변형농산물 중 유전자변형 콩의 혼입유무를 판별할 수 있는 검정기술 개발이 요구되고 있다. 근사미(glyphosate)제초제에 저항성을 나타내는 토양미생물인 Agrobacterium CP4 유래의 5-enolpyruvyl shikimate-3-phosphate synthase(EPSPS) 유전자의 도입여부를 PCR로 진단할 수 있는 특이프라이머를 제작하여 제초제저항성 콩(Roundup Ready Soybean, RRS)을 검정할 수 있는 PCR조건을 확립하였으며 콩의 내재유전자인 lectin유전자와 RRS특이 프라이머를 이용하여 duplex PCR에 의한 제초제저항성 콩의 검정법을 확립하였다. 또한 수입 콩 및 콩나물에 대하여 근사미 제초제 처리로 저항성 개체를 판별하는 생물검정법도 확립하여 저항성 개체의 잎에서 분리한 genomic DNA에 대하여 EPSPS특이 프라이머를 이용하여 분석한 결과 RRS특이적인 PCR밴드를 확인하였다. 또한 수입 콩의 백립중과 종실의 제색을 고려할 때 단일품종이 아닌 여러 품종이 혼합되어 있음을 확인하였다.