• 한국정보기술학회 영문논문지
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• 제10권1호
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• pp.25-36
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• 2020

Motivation: Fibers as the extracellular filamentous structures determine the shape of the cytoskeletal structures. Their characterization and reconstruction from a 3D cellular image represent very useful quantitative information at the cellular level. In this paper, we presented a novel automatic method to extract fiber diameter distribution through a pipeline to reconstruct fibers from 3D fluorescence confocal images. The pipeline is composed of four steps: segmentation, skeletonization, template fitting and fiber tracking. Segmentation of fiber is achieved by defining an energy based on tensor voting framework. After skeletonizing segmented fibers, we fit a template for each seed point. Then, the fiber tracking step reconstructs fibers by finding the best match of the next fiber segment from the previous template. Thus, we define a fiber as a set of templates, based on which we calculate a diameter distribution of fibers.

• Journal of Forest and Environmental Science
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• 제33권1호
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• pp.22-32
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• 2017

An experiment was set to assess the effect of mixed plantings on initial growth and biomass yield of two common plantation tree species of Bangladesh namely Acacia auriculiformis (A) and Gmelina arborea (G). Study was carried out in the nursery bed of the Seed Research Laboratory and Nursery of Institute of Forestry and Environmental Sciences, Chittagong University (IFESCU), Bangladesh during February to November, 2015. The treatments consisted of two pure planting plots (100% A and 100% G) and five mixed planting plots (50%A: 50%G, 35%A: 65%G, 25%A: 75%G, 65%A: 35%G and 75%A: 25%G) of these two species. In nursery, seedlings were raised in a randomized blocks with four replicates of seven treatment plots. Periodic increments on height (cm), collar diameter (cm) and leaf/phyllode number of the seedlings was taken in every month and continued up to 10 months. The growth and biomass yield of seedlings were measured 10 months after the first seed was emerged. The effects of mixed plantation on growth and biomass were compared to that of seedlings grown in pure plantation. At the age of 10 months it was found that G. arborea seedlings were significantly tallest (240.13 cm) when planted with A. auriculiformis in a proportion of 25%A: 75%G, whereas A. auriculiformis were tallest in the pure 100% A plot, with an average mean height of 135.36 cm. Maximum collar diameter (1.38 cm) was recorded for G. arborea in the mixed plots 75%A: 25%G. Fresh and dry weight of shoots and roots of the seedlings were found significantly (p<0.05) highest in 50%A: 50%G plot for G. arborea. G. arborea also showed highest quality index when mixed with A. auriculiformis in a proportion of 50:50, with an average value of 8.96. The results revealed a positive correlation between seedling growth and various planting patterns.

• Spatial Information Research
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• 제18권3호
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• pp.73-83
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• 2010

본 연구는 정지장면의 연속영상간 각 픽셀위치에서 일어나는 통계적 특성을 활용하여 영상을 분할하는 기법을 제안한다. 공간정보의 획득과 분석에서 디지털 영상 처리 기법의 활용은 아주 중요한 의미를 가진다. 특히 디지털 영상의 영역 구분을 위해 다양한 영상 분할(image segmentation) 기법들이 활용되고 있다. 본 연구에서는 선행 연구한 연속프레임 영상의 분광학적 특성 분석의 결과를 바탕으로 연속 프레임 간 Randomness를 활용한 이미지 분할 방법을 제안하였다. 우선 연속 프레임 간 각 화소에 통계학적인 분석 방법을 적용하여 각 화소의 평균과 표준편차 값을 구하고, 이를 통하여 대상 영상에서 가장 신뢰할 만한 화소들을 찾아 씨앗 점(seed point)을 결정하였다. 그리고 이 씨앗 점들을 시작으로 이웃 화소 간 T-test를 실시하였으며, 이를 기반으로 영역 성장(region growing)의 개념을 적용하여 영상을 분할 할 수 있는 기법을 연구하였다. 제안방식의 성능을 검증하기 위하여 실험을 통하여 기존의 방식과 비교분석을 수행하였다. 이러한 실험의 결과 영상분할에서 영상의 단일 프레임을 활용한 것보다 연속 프레임을 활용한 경우가 유리함을 확인 할 수 있었다.

• Journal of Ginseng Research
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• 제36권3호
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• pp.298-307
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• 2012

Panax ginseng has been cultivated for centuries, and nine commercial cultivars have been registered in Korea. However, these nine elite cultivars are grown in less than 10% of ginseng fields, and there is no clear authentication system for each cultivar even though their values are higher than those of local landraces. Here, we have developed 19 microsatellite markers using expressed gene sequences and established an authentication system for all nine cultivars. Five cultivars, 'Chunpoong', 'Sunpoong', 'Gumpoong', 'Sunun', and 'Sunone', can each be identified by one cultivar-unique allele, gm47n-a, gm47n-c, gm104-a, gm184-a (or gm129-a), and gm175-c, respectively. 'Yunpoong' can be identified by the co-appearance of gm47n-b and gm129-c. 'Sunhyang' can be distinguished from the other eight cultivars by the co-appearance of gm47n-b, gm129-b, and gm175-a. The two other cultivars, 'Gopoong' and 'Cheongsun', can be identified by their specific combinations of five marker alleles. This marker set was successfully utilized to identify the cultivars among 70 ginseng individuals and to select true F1 hybrid plants between two cultivars. We further analyzed the homogeneity of each cultivar and phylogenetic relationships among cultivars using these markers. This marker system will be useful to the seed industry and for breeding of ginseng.

• Journal of Ginseng Research
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• 제35권4호
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• pp.504-513
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• 2011

In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.

• 대한본초학회지
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• 제31권4호
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• pp.61-69
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• 2016

Objectives : This study is to propose discriminative keys with stereoscope for minute seed herbs easily mixed up, but hard to discriminate by naked eyes: Alli Tuberosi Semen(ATS)✳Alli Fistulosi Semen(AFS), Malvae Semen(MS)✳ Abutili Semen(AS), Plantaginis Semen(PS)✳Schizonepetae Semen(SS).Objectives : We reviewed the description of original plants and medicinal herbs from literature. The specimen of original plant were collected, determinated and compared to samples in the market. Primary classification was performed with naked eyes. and we found out other discrimination keys for non-distinctive herbs with stereoscope. Discrimination keys were set as the morphological criteria of authentic herbs, percentage of adulteration, and standards for discriminating genuine herbs from adulteration.Results : 1) ATS is bigger, has reticulate pattern on protuberant side and unique garlic chives taste. AFS which is usually mixed up, is smaller, has 1~2 ridges and unique welsh onion taste. 2) MS is smaller, has no villus, but its length of bumps are similar. AS easily mixed up, is bigger and has white villus. Its upper side bump is longer and more sharp. 3) PS has dent at middle of the back side. It becomes very sticky when it is put or boiled in water. SS which can be mixed up has no dent and low viscosity compared to PS.Conclusions : With this result, we propose discriminative keys which can identify the original plants and processed herbal state of six herbs. Because minute medicinal herbs are hardly distinguishable by sensory test, It is essential to differentiate by using stereoscope.

• 식물병연구
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• 제13권2호
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• pp.82-87
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• 2007

박과 작물에 발생하는 종자전염성 막대형 바이러스인 CGMMV, KGMMV, ZGMMV 3종의 단독 및 동시 VC/RT-PCR 유전자 진단법을 개발하였다. CGMMV 등 3종의 바이러스에 대한 동시진단용 조합선발을 위하여 12조합 중에서 동시 진단용 프라이머 조합 CGMMV-C724, KGMMV-K513, ZGMMV-Z407A를 선발하였다. CGMMV등 3종에 대한 triplex VC/RT-PCR 유전자 진단법은 박, 수박, 오이, 멜론, 쥬키니 호박, 애호박, 담배(N. benthamiana) 작물의 식물체 즙액과 프라이머 간에 간섭현상 없이 특이적으로 진단되었다.

• Journal of Biosystems Engineering
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• 제26권4호
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• pp.379-384
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• 2001

To enhance the smooth graft-taking of grafted seedlings, the excessive evapotranspiration from grafted seedlings just after grafting should be avoided. A measurement system of the evapotranspiration rate of grafted seedling under artificial lighting was set up to investigate the effect of physical environment on the evapotranspiration and graft-taking characteristics of grafted seedlings quantitatively. The evapotranspiration rate of grafted seedlings affected by relative humidity and light intensity were analyzed using the measurement system. The hypocotyl of watermelons (Citrullus vulgaris cv. Sweetdew, Hungnong Seed Co.) was slantly cut and then inserted into a hole on the stem of rootstock (Lagenaria siceraria cv. FR-King, Hungnong Seed Co.). Grafted seedlings were healed and joined for 5 days under cool-white fluorescent lamps (FL20SEX-d/18, Keumho Electric Co.) with photoperiod of 12h$.$d$\^$-1/ except dark period for one day after grafting in a closed graft-taking enhancement system developed by Kim(2000). The evapotranspiration rate and graft-taking of grafted seedling at air temperature of 23$\^{C}$ and air current speed of 0.1m$.$s$\^$-1/ was highly affected by relative humidity. But light intensity showed higher effect on the stem length of scion than relative humidity. In conclusion, it was suggested that relative humidity should be controlled at higher than 90% with photosynthetic photon flux of 50$\mu$mol$.$m$^2$$.$s$\^$-1/ to increase the survival of grafted seedlings and to produce healthy seedlings.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1470-1475
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• 2009

The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-$\beta$-glucoside, cyanidin-3-O-$\beta$-glucoside, and petunidin-3-O-$\beta$-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil-$5C_{18}$-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at $35^{\circ}C$. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-$\beta$-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.

• 한국전자통신학회논문지
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• 제13권4호
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• pp.787-794
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• 2018

지구 온난화로 인해 홍수나 집중 호우와 같은 자연 재해들이 증가하고 있다. 이러한 자연 재해들이 미리 그리고 효과적으로 인지될 수 있다면 재해로 인한 많은 피해들을 미리 막을 수 있을 것이다. 최근 비주얼 센서기술의 발전을 바탕으로 재해를 예방하기 위해 하천을 포함한 다양한 자연환경을 감시하는데 비주얼 센서 기술을 적용하는 연구들이 많이 진행되고 있다. 이 논문에서는 비주얼 센서 네트워크 기술을 이용한 하천 감시시스템에 적용 가능한 하천 영상에서 강물 영역을 분할하는 방법을 제안한다. 제안하는 방법에서는 먼저 최소 신장트리 알고리즘을 이용하여 영상을 세밀하게 분할한다. 그리고 강물 영역과 배경 영역에 대한 사전 정보를 이용하여 초기 영역을 설정하고 유사한 영역을 병합하여 각 영역을 확장함으로써 강물 영역을 분리한다. 실험결과는 제안하는 방법이 간단하면서도 정확하게 강물 영상에서 강물 영역을 분리하는 것을 보여준다.