• Journal of Plant Biotechnology
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• 제47권3호
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• pp.209-217
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• 2020

The presence of high amounts of seed storage proteins (SSPs) improves the overall quality of soybean seeds. However, these SSPs pose a major limitation due to their high abundance in soybean seeds. Although various technical advancements including mass-spectrometry and bioinformatics resources were reported, only limited information has been derived to date on soybean seeds at proteome level. Here, we applied a tandem mass tags (TMT)-based quantitative proteomic analysis to identify the significantly modulated proteins in the seeds of two soybean cultivars showing varying protein contents. This approach led to the identification of 5,678 proteins of which 13 and 1,133 proteins showed significant changes in Daewon (low-protein content cultivar) and Saedanbaek (high-protein content cultivar) respectively. Functional annotation revealed that proteins with increased abundance in Saedanbaek were mainly associated with the amino acid and protein metabolism involved in protein synthesis, folding, targeting, and degradation. Taken together, the results presented here provide a pipeline for soybean seed proteome analysis and contribute a better understanding of proteomic changes that may lead to alteration in the protein contents in soybean seeds.

• 농촌지도와개발
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• 제25권1호
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• pp.31-44
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• 2018

This is a case study on "the Project for Production Facility and Technical Support of Processed Seed-Potato (2008-2010)" based on the Value Chain Analysis (VCA) used to create and plan International development cooperation projects. The project is the earliest model of Creating Shared Value (CSV) in the agricultural sector. For the case analysis, a framework was established to assess the effectiveness and impact of the CSV project based on the main factors of the VCA. As a result of the assessment, project participation by OSI was able to increase the overall utility by developing the Vietnamese potato processing industry and meeting consumer demand. Furthermore, it formed a business model to promote win-win cooperation and upgraded the value chain of the potato industry. In addition, it contributed to the improvement of incomes and the quality of life of farmers and communities by providing technical guidance and purchase of contracts, as well as labor division and cooperation with other activity supporters.

• 농업과학연구
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• 제39권1호
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• pp.111-116
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• 2012

Nondestructive evaluation of seed viability is one of the highly demanding technologies for seed production industry. Conventional seed sorting technologies, such as tetrazolium and standard germination test are destructive, time consuming, and labor intensive methods. Near infrared spectroscopy technique has shown good potential for nondestructive quality measurements for food and agricultural products. In this study, FT-NIR spectroscopy was used to classify normal and artificially aged lettuce seeds. The spectra with the range of 1100~2500 nm were scanned for lettuce seeds and analyzed using the principal component analysis(PCA) method. To classify viable seeds from nonviable seeds, a calibration modeling set was developed with a partial least square(PLS) method. The calibration model developed from PLS resulted in 98% classification accuracy with the Savitzky-Golay $1^{st}$ derivative preprocessing method. The prediction accuracy for the test data set was 93% with the MSC(Multiplicative Scatter Correction) preprocessing method. The results show that FT-NIR has good potential for discriminating non-viable lettuce seeds from viable ones.

• Journal of Ginseng Research
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• 제30권2호
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• pp.78-81
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• 2006

Freshly harvested, immature (green) seeds of North American ginseng (Panax quinquefolius L.) were stratified for up to 3 years in plastic pails in controlled environment rooms at $5{\pm}1^{\circ}C$ for 9 months and then $21{\pm}2^{\circ}C$ for 3 months (Trt. 1, regular stratification), or continuously at $-2{\pm}0.2^{\circ}C$ (Trt. 2), or continuously at $3{\pm}0.2^{\circ}C$ (Trt. 3). During stratification at -2 and $3^{\circ}C$ embryos did not grow. On seeding in the field embryos grew rapidly and resultant seedlings were comparable to those from regularly stratified seed. Seedling emergence rate was acceptable at the industry expected rate of 68% after one year of storage, but not after two years storage when it declined to 17.5%. Seed rot was so severe in year 3 that no planting was carried out. Seedling and second year growth were similar at the three stratification temperatures; most importantly, root dry weight (economic yield) was similar. Low-temperature storage of freshly-harvested North American ginseng seed is an acceptable method for short-term retention of propagating material.

• 펄프종이기술
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• 제36권4호
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• pp.41-48
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• 2004

To make a basepaper for multipurposed seed paper, old news print (ONP) and mixed office waste paper (MOW), modified and unmodified with a commercial cellulase, were investigated. Each handsheet was applied with different chemicals such as insecticides, germicides and herbicides. The interactions behaviors of chemicals used with base papers were evaluated by means of the contents of chemicals impregnation and dissolving behaviors in water and soil. The ONP and MOW treated with the cellulase had higher impregnation and dissolving capacities in both water and soil than untreated ONP and MOW. However, the modified ONP showed lower impregnation and dissolving capacities compared to the modified MOW. The content of impregnation of chemicals would be affected with the degree of microfibrils produced by the modification treatment. Otherwise, dissolving capacities of chemicals depend on the affinity of the residual lignin in the paper.

• 한국식품저장유통학회지
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• 제15권6호
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• pp.852-858
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• 2008

연자육 분말의 첨가량에 따른 식빵 반죽의 물리적 특성을 알아보기 위하여 연자육 분말을 첨가한 반죽의 팽창력, ferinogram, amylogram, extensogram, 반죽의 pH와 식빵의 외관 등을 조사하였다. 연자육 분말의 일반성분 함량은 수분 7.74%, 조단백질 20.15%, 조지방 2.11%, 조회분 4.34%, 조섬유소 2.78%이였다. 반죽의 팽창력 결과 대조구에 비해 연자육 분말 첨가구가 팽창력이 좋았으며, 반죽의 farinogram 특성은 연자육 분말 첨가량이 증가할 수 록 흡수율이 다소 증가하였고, 반죽도달시간은 대조구에 비해 다소 짧아졌다. 연자육 분말 첨가량의 증가에 따라 반죽형성 시간과 안정도는 짧아졌고 연화도는 커지는 경향을 보였다. Amylogram의 호화특성에서 최고점도온도의 경우 연자육 분말 첨가구와 대조구가 같았으며, 연자육 분말 첨가량이 증가함에 따라 최고점도는 증가하였다. Extensogram 특성은 연자육 분말 첨가량이 증가할 수 록 신장도는 감소하였고, 저항도는 증가하였으며 이에 따라 R/E 값이 증가하였다. 반죽 후 대조구의 pH는 6.06이었고, 연자육 분말 첨가구의 pH는 $6.19{\sim}6.21$였다. 발효시간 경과에 따른 대조구의 pH는 서서히 낮아지는 경향을 보였으며, 연자육 분말 첨가구에서는 발효시간 90분까지는 서서히 낮아졌으나 90분 이후부터 pH가 급격히 낮아졌다. 이로써 연자육 분말의 첨가량이 식빵 제조용 반죽의 리올로지에 크게 영향을 미치는 것으로 확인 되었다.

• 대한토목학회논문집
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• 제41권3호
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• pp.289-296
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• 2021

국내 건설산업은 에너지와 자원의 사용 및 오염물질 배출과 같은 환경 부담을 줄이고 쾌적한 환경을 조성하기 위한 목적으로 건축물의 환경성을 평가하는 녹색건축 인증제도를 시행하고 있다. 하지만 인증획득 절차의 시간이 오래 걸리고 과정이 복잡하며 인증 항목별로 자재의 경제성을 고려할 수 없는 점으로 인해 실무에서는 인증에 어려움을 겪는 문제점이 있다. 따라서 본 연구에서는 녹색건축인증의 문제점을 해결하기 위해 녹색건축 인증제도 인증 항목 중 실내공기 오염물질 저방출 제품의 적용을 그 대상으로 하고 생애주기비용 개념을 고려하여 자재 데이터베이스를 구축하였으며 경제성 평가의 최적화 기법으로는 유전 알고리즘을 활용하여 최적의 자재를 선정할 수 있는 경제성 평가모델을 개발하였다. 이를 실제 비주거용 건축물 사례에 적용하여 기존 비용과 비교한 결과 평균 74.5 %의 비용 절감률을 나타내어 본 모델을 녹색건축인증에서 경제적 효율성이 있는 도구로써 활용 가능할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권1호
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• pp.86-94
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• 2015

This experiment was conducted to evaluate the chemical composition and amino acid (AA) digestibility of sunflower seed meal (SFSM) and to use this data to develop prediction equations for estimating AA digestibility for growing pigs. Ten SFSM were collected from five provinces in China. Twelve barrows ($38.8{\pm}4.6kg$), fitted with ileal T-cannula were allotted into two $6{\times}6$ Latin square designs. Each of six experimental periods comprised a 5-d adaption period followed by a 2-d collection of ileal digesta. The ten test diets contained 50% SFSM as the sole source of AA. Another nitrogen-free diet was used to measure the basal endogenous losses of crude protein (CP) and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. There was considerable variation (CV>10%) among the ten SFSM in chemical composition (dry matter [DM]). The concentration of CP and ether extract (EE) ranged from 29.33% to 39.09% and 0.88% to 11.33%, respectively. Crude fibre (CF), neutral detergent fibre and acid detergent fibre ranged from 21.46% to 36.42%, 38.15% to 55.40%, and 24.59% to 37.34%, respectively. There was variation among the ten SFSM in apparent ileal digestibility (AID) and standardized ileal digestibility (SID) for lysine and threonine, which ranged from 63.16 to 79.21 and 55.19% to 72.04% for AID and 67.03% to 82.07% and 61.97% to 77.01% for SID, respectively. The variation in CP and methionine ranged from 60.13% to 74.72% and 74.79% to 88.60% for AID and 66.70% to 79.31% and 77.16% to 90.27% for SID, respectively. Methionine was a good indicator to predict AA digestibility. These results indicate that conventional chemical composition of SFSM was variable (CV>10%) among the ten SFSM (DM). The results of AID, SID and prediction equations could be used to evaluate the digestibility of SFSM in growing pigs.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.162.1-162.1
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• 2015

The use of plasma has increased in bio-application field in recent years. Particularly, water treated by arc discharge or atmospheric pressure plasma has been actively utilized in bio-industry. In this study, we have developed a plasma activated water generating system. For this system, two kinds of plasma sources; dielectric barrier discharge (DBD) plasma and arc discharge plasma have been used. The discharge energy was calculated using the breakdown voltage and current, and the emission spectrum was measured to investigate the generated reactive species. We also analyzed the amount of reactive oxygen and nitrogen species in water using the chemical methods and nitric oxide sensor. Finally, the influence of plasma generated reactive species on the germination and growth of spinach (Spinacia oleracea) was investigated. Spinach is a green leafy vegetable that contains a large amount of various physiologically active organic compounds. However, it is characterized with a low seed germination rate.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.102-105
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• 2002

Seventy-two crossbred pigs weighing an average of 41.5 kg were assigned on the basis of sex, weight and litter to one of four dietary treatments in a factorial (4 treatments${\times}$2 sexes) arrangement. The control diet was based on barley and soybean meal while the experimental treatments consisted of diets in which a portion of the dietary protein was supplied by 20% of a 50:50 blend of extruded ($130^{\circ}C$ for 20 to 25 sec) peas and full-fat canola seed, 20% of a 50:50 blend of unextruded peas and full-fat canola seed or a diet containing 10% peas, 6% canola meal and 4% canola oil (to equal the level of canola oil provided by 10% whole canola seed). Digestibility coefficients for dry matter, crude protein and gross energy were significantly higher (p<0.05) for the control diet than for the other three diets. Extrusion produced no beneficial effects (p>0.05) on nutrient digestibility and there were no differences in digestibility between the diet based on intact canola seed compared with the diet containing canola meal and oil. Choice of protein supplement had no significant effects on gain, feed intake or feed conversion during the grower or finisher phases and over the entire experimental period. Extrusion of the pea-canola blend produced no beneficial effects on pig performance as the performance of pigs fed either the extruded or unextruded blend of peas and canola seed was similar. In addition, the performance of pigs fed diets containing intact canola seed was similar to that of pigs fed canola meal and oil. Castrates gained faster and consumed more feed than gilts (p<0.05). However, their feed conversion was poorer than that of the gilts during the finisher period. There were no significant differences in carcass traits between pigs fed the control and any of the experimental treatments. Extrusion had no effect on carcass traits and the carcasses of pigs fed canola meal and oil did not differ from those of pigs fed whole canola seed. Castrates had a significantly lower dressing percentage, lower estimated lean yield but greater loin fat depth than gilts (p<0.05). The results of this experiment indicate that peas in combination with canola seed or canola meal are an acceptable alternative to soybean meal as a protein supplement for use in growing-finishing swine diets. Extrusion did not appear to have any beneficial effects on the nutritional value of the canola seed-pea blend as nutrient digestibility, growth performance and carcass traits were similar for pigs fed the unextruded blend of peas and canola seed compared with the extruded product. Since the process adds to the cost of the raw products, its use is unlikely to be economical.