• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.1
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• pp.55-62
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• 1994

The germplasms of naked barley evaluated for starch content were 137 crossing blacks and progeny lines obtained from the breeding program of the Wheat and Barley Research Institute, 540 Tabdong Kwonsungu Suwon, in the 1988/1989 season. Starch content was distributed between 49.15 and 62.50% with a mean of 57.20%. It was high in the bred lines and the Uz type lines than the imported, uz type or lax type ones. The range observed for seed coat weight was 1.12-3.02mg and the mean seed coat weight was 1.88mg. The seed coat weight ratio to kernel weight was measured between 4.0 and 8.7% with a mean of 6.5%. The varieties differ greatly in the characteristics depending on the plant type and origin of germplasms. The variation observed with protein content ranged from 9.60 to. 15.90% while the average was 12.86%. Starch content was negative relationships with kernel weight, seed coat weight, seed coat weight ratio., kernel width, kernel thickness and protein content, and starch content was higher in smaller kernels within a variety. The weight and weight ratio. of seed coat were positively correlated with the width, thick-ness of kernels, whilst they were negatively correlated with starch content. The heritability of starch content was 30.0%.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.118-123
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• 2020

The black seed coat of soybeans contain anthocyanins which promote health. However, mature soybean seeds contain anti-nutritional factors like lipoxygenase, lectin and Kunitz Trypsin Inhibitor (KTI) proteins. Furthermore, these seeds can be used only after the genetic elimination of these proteins. Therefore, the objective of this study was to develop novel soybean genotypes with black seed coat and triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele) for lipoxygenase, lectin, and KTI proteins. From a cross of parent1 (lx1lx2lx3/lx1lx2lx3, ti/ti, Le/Le) and parent2 (lx1lx2lx3/lx1lx2lx3, Ti/Ti, le/le), 132 F₂ seeds were obtained. A 3:1 segregation ratio was observed during F₂ seed generation for the inheritance of lectin and KTI proteins. Between a cross of the Le and Ti genes, the observed independent inheritance ratio in the F₂ seed generation was 9: 3 : 3 : 1 (69 Le_Ti_: 32 leleTi_: 22 Le_titi: 9 leletiti) (χ²=2.87, P=0.5 - 0.1). From nine F₂ seeds with triple recessive alleles (lx1lx1lx2lx2lx3lx3, titilele genotype), one novel strain posessing black seed coat, and free of lipoxygenase, lectin and KTI proteins, was selected. The seed coat color of the new strain was black and the cotyledon color of the mature seed was green. The weight of 100 seeds belonging to the new strain was 35.4 g. This black soybean strain with lx1lx1lx2lx2lx3lx3, titilele genotype is a novel strain free of lipoxygenase, lectin, and KTI proteins.

• Journal of Ginseng Research
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• v.32 no.3
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• pp.257-263
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• 2008

This study was carried out to obtained the basic information for seed coat dehiscence and seedling performance according to seed size of eight ginseng cultivars. Seed size was separated into four grade(upper 5.0, 5.0-4.7, 4.7-4.0, and under 4.0mm in diameter). Seed coat dehiscence and survival ratio of seedling were not affected by seed size. Aerial parts growth of seedling such as stem length, stem diameter, and leaf area of seedling were larger seed(upper 5.0 mm) better than those of small seeds(4.7-4.0, and under 4.0 mm in diameter). Also root part growths such as root diameter, and fresh weight were larger seed(upper 5.0 mm) higher than those of small seeds(4.7-4.0, and under 4.0 mm in diameter). However, root length was not affected by seed size. Growth status of aerial and root parts of 'unpoong' 'umpoong' and 'opoong' were better than those of other cultivars in each grade of seed sizes. The growth of characteristics of 'hunpoong' and 'hungsun' were less than those of other cultivars.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.269-272
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• 1998

Characteristics of seed and plant growth of the nine local collections of the medicinal plant, Agastache rugosa were evaluated. Seed size of the collection from Garyungsan was larger than the others. Seeds of Damyang collection were the smallest in size. Seeds of the collections were mostly oval shaped. There were no differences between brown and black seed coat color groups in seed width, ratio of seed length to seed width and 1,000 seed weight. Stem lengths of Garyungsan and Bongpyung collections were 99.9 and 95.6 cm, respectively, and were longer than Damyang and Jindo collections. Garyungsan collection had long leaf shape. Leaf size was the smallest for Damyang collection, but was the largest for Bongpyung collection. Inflorescence length of Mokpo collection (15.6 cm) was longer than the average over all collections (12.3 cm). Damyang collection was the shortest (10.1 cm) in inflorescence. Top dry weight of each collection from Soonchun, Bongpyung, Mokpo, Jindo, and Gurye was greater than the average over all collections (20.38 g/plant). Jinju and Damyang collections had less top dry matter than others. There was no difference in the growth traits examined between brown and black seed coat groups. The brown seed group was greater in leaf weight than the black seed group. However, these two groups were not different in stem and inflorescence weight. Collections of A. rugosa from Bongpyung, Soonchun, and Mokpo were promising for commercial cultivation because of their great top dry weight, especially in leaf and inflorescence.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.382-388
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• 1994

For the comparision of the differences in dry matter weight and the chemical composition of soybean seeds in relation to utilization styles and storage durations, the seed coats and cotyledons including embryo axis separated from the seeds of 3 recommended cultivars stored for 3 years and of 5 recommended cultivars and 2 local varieties with black seed coat and green cotyledons were observed in dry weight, germination percentage, protein, oil, soluble sugar, oilgosaccharide, and starch. In the seeds stored for 3 years, dry weight and germination rate were decreased distinctively. This decrease of seed dry matter resulted from the decrease of the cotyledon weight, and the ratio of seed coat to whole seed was increased. For the change of chemical compositions by the decrement of seed dry weight during the storage, protein, oil, and carbohydrate contents of the seed coat were decreased, and protein content of the cotyledon was markedly decreased in the seeds stored for 3 years. The ratio of seed coat to whole seed was higher in small seed than large seed cultivars. In the local varieties, protein and oil contents were 43.59% and 18.25% in the cotyledons, respectively, which were lower than those of recommended cultivars. But starch and sugar contents were 5.70% and 11.58%, respectively, which were higher than those of recommended cultivars. Sucrose, raffinose and stachyose contents were also higher in the cotyledon of the local varieties.

• Journal of Environmental Science International
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• v.24 no.12
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• pp.1681-1689
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• 2015

This study was conducted to investigate days after anthesis (DAA) and fruit after-ripening period (FAP) for seed-harvesting of high quality watermelon seeds. Fruit weight and number of seed per fruit increased according to DAA, while those did not significant about FAP. Ratio of cotyledon at whole seed was higher about 2 to 4% compared to seed coat irrespective of DAA and FAP. Germinability of watermelon was not a significant effect by DAA, however, it had differences by FAP. Percent of germination (PB) was below 50%, when 30 days maturated fruits after anthesis was omitted ripening, while PB was increased to 92% by ripening. In addition, seeds at DAA 40 and FAP 20 were higher general seedling vigors (hypocotyl length, diameter etc.) in BP test. Results indicated that considering seed productivity, it had maximized seed viability at DAA 40 and FAP 20.

• Journal of Ginseng Research
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• v.5 no.2
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• pp.85-91
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• 1981

Ginseng seeds were gathered from 3,4,5 and 6 years of age and were classified into four qroups (below 4mm, 4∼5mm, 5∼6mm and above 6mm in across sieve). They were sown in seedling bed and some characters were investigated in each qroup of seed size. 1. The distribution of seed size of below 4mm, 4-5mm, 5-6mm and 6mm were 23.7%, 60.8%, 12.4% and 4.5%, respectively. 2. The ratio of seed coat dehiscence was not affected by seed size but emergence ratio and emerging vigor were superior in large seed. 3. The large seed showed superiority in stem length, stem diameter, leat and also in root length, root diameter and root weight. but diseased root was not affected by seed size. The effect of age(seed harvest) was not significant on all those characters.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.267-271
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• 2010

A new black seed coat soybean cultivar, 'Daeheug', was developed at the National Institute of Crop Science (NICS) in 2007. The breeding goal for black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Daeheug' was selected from the cross between 'Daehwang' which had yellow seed coat, large seed size, and late maturity, and 'Milyang 79' which was lodging tolerant, and SMV resistant with medium seed size, black seed coat, and yellow cotyledon. The preliminary, advanced and regional yield trials for evaluation and selection of this cultivar were carried out from 2003 to 2007. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, spherical flattened seed shape, oval leaf shape and large seed size (34.3 g/100 seeds). In maturity, 'Daeheug' was 2 days later than the check cultivar 'Ilpumgeomjeongkong'. In the seed quality such as isoflavone contents ($635{\mu}g/g$), and anthocyanin contents ($11.2m{\ell}/g$ of seed coat), 'Daeheug' was better than the check cultivar. It has good adaptability for cooking with rice in ratio of water absorption and seed hardness. Specially, it has good processability for soybean tea with 2.13 absorbance at 530 nm, scavenge activity of DPPH radical was high, and contents of total phenolics was $1,263{\mu}g/m{\ell}$. The average yield of 'Daeheug' was 2.26 ton per hectare in the regional yield trials (RYT) carried out in six locations of Korea from 2005 to 2007.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.316-316
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• 2022

Azuki bean is the important bean crop in Korea, and is traditionally used as a filling material for red bean porridge, rice cakes, and bread, as well as for sediment. So far, the National Insititute of Crop Science has developed azuki bean varieties for various uses, such as for sediment, tea, and azuki bean sprouts. Among azuki bean elite lines, 25 strains were used to analyze the correlation between red bean quality characteristics and sediment yield. The crude protein of red beans was 25.0-28.9%, ash content was 3.8-5.3%, and fat was 0.4-1.0%. As for the appearance quality characteristics of azuki bean, one hundred seed weight was 11.1-19.5 g, the length of seed was 6.45-8.49 mm, the width seed was 4.84-6.45 mm, and the withd/lenght ratio was 0.72-0.89. When the azuki bean were boiled, the thickness of the seed coat was 0.14-0.27 mm and the length ratio of seed was 0.65-0.76 mm, showing that the length of seed was slightly larger than the width. The yield of azuki bean sediment was 239-284% for whole red bean paste, and 144-203% for fine azuki bean paste. As a result of analyzing the correlation between the quality characteristics of azuki beans, such as protein, husk and seed weight, and the yield of azuki bean sediment, the yield of whole azuki bean sediment showed a negative correlation with the seed coat thickness and the length ratio of the original grain at -0.62 and -0.45, respectively. The yield of fine sediment showed a negative correlation with the length ratio of whole azuki beans at -0.49, and a positive correlation with that of white beans at 0.41. However, protein and ash content did not show any correlation with sediment yield. In view of the above results, it is thought that it would be better to have a high seed weight or a low width/length ratio of seed to develope azuki bean cultivar for sediment.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.668-673
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• 2010

A new black seed coat soybean cultivar, 'Heugseong' was developed at the National Institute of Crop Science (NICS) in 2008. The goal to breed the black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Heugseong' was selected from the cross between 'Gnome85', showing yellow seed coat, lodging tolerance, and high yield, and 'Cheongja', showing green cotyledon, and black seed coat. The preliminary, advanced and regional yield trials for this cultivar were carried out from 2004 to 2008. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, elongated seed shape, oval leaf shape and large seed size (29.2 grams per 100 seeds). It was 4 days later in maturity than the check cultivar 'Ilpumgeomjeongkong'. 'Heugseong' was better than the check cultivar in the seed quality of isoflavone contents(1,913 ${\mu}g/g$). It has good adaptability for cooking with rice in ratio of water absorption and seed hardness, for physics of black tofu, and for fermented black soybean paste. The average yield of 'Heugseong' was 2.37 ton per hectare in the regional yield trials carried out in six locations of Korea from 2006 to 2008.