• Title/Summary/Keyword: Seed Number

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RAV1 Negatively Regulates Seed Development by Directly Repressing MINI3 and IKU2 in Arabidopsis

  • Shin, Hyun-young;Nam, Kyoung Hee
    • Molecules and Cells
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    • v.41 no.12
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    • pp.1072-1080
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    • 2018
  • A plant-specific B3 domain and AP2 domain-containing transcription factor, RAV1 acts as a negative regulator of growth in many plant species and its transcription was down-regulated by BR and ABA. In this study, we found that RAV1-overexpressing transgenic plants showed abnormally developed ovules, resulting in reduced seed size, weight, and number in a silique. Interestingly, the endogenous expression of RAV1 fluctuated during seed development; it remained low during the early stage of seed development and sharply increased in the seed maturation stage. In plants, seed development is a complex process that requires coordinated growth of the embryo, endosperm, and maternal integuments. Among many genes that are associated with endosperm proliferation and embryo development, three genes consisting of SHB1, MINI3, and IKU2 form a small unit positively regulating this process, and their expression was regulated by BR and ABA. Using the floral stage-specific RNAs, we found that the expression of MINI3 and IKU2, the two downstream genes of the SHB1-MINI3-IKU2 cascade in the seed development pathway, were particularly reduced in the RAV1-overexpressing transgenic plants. We further determined that RAV1 directly binds to the promoter of MINI3 and IKU2, resulting in their repression. Direct treatment with brassinolide (BL) improved seed development of RAV1-overexpressing plants, but treatment with ABA severely worsened it. Overall, these results suggest that RAV1 is an additional negative player in the early stages of seed development, during which ABA and BR signaling are coordinated.

Genetic Variation of Flower Production in Breeding Seedling Seed Orchards of Quercus acuta and Q. glauca

  • Jeon, Koeun;Ro, Hee Seung;Kim, Ye-Ji;Gu, Da-Eun;Park, Ji-Min;Ryu, Sungryul;Kang, Kyu-Suk
    • Journal of Forest and Environmental Science
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    • v.38 no.2
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    • pp.102-109
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    • 2022
  • This study was conducted to test the significant difference of fertility variation among families and to select superior families for acorn production in the breeding seedling seed orchards (BSSOs) of Quercus acuta and Quercus glauca. The seed orchards were located in Jeju island and established by seedlings raised from selected parents for genetic testing in 2006. In the spring of 2021, the numbers of female and male flower were counted from 5 to 10 individuals per family in the BSSOs. To test statistical significance of which parameter is not satisfied through the normality test, we used a nonparametric analysis. Correlation analysis was performed to quantify the association between female and male flower production. As the results, the significant difference of flower production among families was found in both seed orchards. The averages of female flower production were 65.3 and 181.9 in Q. acuta and Q. glauca. The positive Spearman's rank correlation was existed between male and female flower production. Broad-sense heritability on female and male flower production were 0.191 and 0.147 in Q. acuta, and 0.285 and 0.068 in Q. glauca, respectively. Sexual asymmetry (e.g., maleness index) between female and male, and contribution variation among families (e.g., parental balance) were analyzed to find reasonable alternatives in the management of seed orchards. Effective population size of seed crops was predicted as a concept of status number. Loss of gene diversity (accumulation of group coancestry) would not be alarming in the BSSOs. Our results would be helpful to select breeding materials for establishing new seed orchards and to supply genetically improved seeds of evergreen oaks, which is one of the backbones of the strategy of carbon sink in the 2050 Carbon Neutrality of Korea Forest Service.

Studies on the Ecological Characteristics for the Plant Types in the Peanut (Arachis hypogaea L.) N. Effect of Vinyl-and Non-mulching on Growth among Peanut Plant Types (땅콩의 초형별 생태적 특성에 관한 연구 IV. 비닐피복재배가 초형별 생육에 미치는 영향)

  • Lee, Jung-Il;Park, Yong-Hwan;Park, Yeon-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.361-365
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    • 1986
  • This study was conducted to investigate the growth characteristics for each of botanical types of peanut (Arachis hypogaea L.) under vinyl-and non-mulching in cultural limiting region. Ten peanut varieties pertaining to virginia-large seed, virginia-small seed, spanish, valencia, shinpung type were used in this study. Main stem length was in order of valencia, spanish, virginia-small seed, shinpung, virginia-large seed type under vinyl-and non-mulching. By vinyl-mulching, the maximum growth stage of main stem was shortened by 20 days than that of non-mulching. Number of branches per plant was distinguished among botanical types. Numbers of those were virginia-small seed 28, virginia-large seed 27, shinpung 15, spanish 13, valencia 7 under vinyl-mulching and were virginia-small seed 22, virginia-large seed 21, spanish 12, spinpung 12, valencia 5 under non-mulching. The longeset branch length was in order of valencia, spanish, spinpung, virginia-large seed, virginia-small seed type under vinyl-and non-mulching and velencia type was the longest of the botanical types.

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Studies on the soybean varieties in Korea. -IV relationships between the seed yields and some characteristics on the several sowing dates- (대두의 품종에 관한 연구 -제4보 파종기별 수량과 제특성과의 관계-)

  • Kwon-Yawl Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.2
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    • pp.30-37
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    • 1964
  • Experiments were carried out to clarify the relationships between seed yields and some ecological characteristics, seed yields and some morphological characteristics, and the relationships among the varietal yields on the several sowing plots when grown under different climatic conditions. Twenty-four soybean varieties were used as the material, selected at random from 138 varieties which were cultivated the year before. These varieties were grown in the Experimental Farm, Chinju Agricultural College, Kyung Sang Namdo, Korea, and seed sowing was conducted at 5 times, from April to July, in 1962 and 1963. The results obtained are summarized as follows: 1. Correlations between seed yields and some ecological characteristics on the sowing date bases are shown in Table 1. Among some ecological characteristics, it was observed that there wert close relationships between seed yields and days to flowering, and between seed yields and the total growing length, except on the late sowing, i.e. July sowing plots, However, there was no clear correlation between seed yields and durations of flowering, and between seed yields and days from flowering to maturity. 2. Correlations between seed yields and some morphological characteristics on the sowing date bases are shown in Table 2. From this table, it was also observed that there were some close relationships between seed yields and branch number for plant, stem diameter, plant weight, pod number per plant, and grain number per plant, but there was no clear relationship between seed yields and stem length, and between seed yields and 100 grain weight, on every sowing plot. 3. Relations between varietal yields and the sowing dates are shown in Table 3. Our leading varieties such as Ulsan, Sangdoo, Changdan Backmok, Iksan, Haman, Barchongdeo, and Anion Daerip, which belong to the maturity groups of IV, V, and VI, have been significantly high in yield at each sowing date plot, but the extreme early varieties, that frequently are damaged by insects, moisture and diseases, and the extreme late varieties, that frequently are caught by frost suffering reduced yield, are showed a lower yield than the others. However, extreme early varieties, for instance, Black Hawk, an American variety, and Chung Buk Baek, a Korean early variety, showed high productivity on the late sowing, June and July sowing plots, and Do san No. 6, which is late Japanese variety, showed a high yield on the extreme early sowing, i.e. April sowing plots. 4. Relations between seed yields and the sowing dates on the varietal bases are shown in Table 4. It was observed that, the early varieties did not differ much in yields at the various sowing dates while the yields of late varieties decreased progressively with subsequent dates. However, the extreme late varieties, such as Tamanishiki, that frequently are caught by frost suffering reduced yield, may be difficult to harvest at this location. The results in these experiments showed that in most cases maximum yields were obtained when leading varieties, which are medium or late varieties, were shown in Mid-May, and progressively lower yields were produced from June and July sowings. On the other hand, the late sowings of the early varieties, such as Black Hawk and Chung Buk Baek, in these experiments showed favorable yields compared with the others. From these results, we can predict that our local medium or late varieties produce higher yields at this location than was produced by early varieties in most of the sowing dates, especially the May sowing, and early varieties such as Black Hawk and Chung Buk Baek should be used in the late sowing, June sowing time.

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Comparison of Triglyceride Composition between Red Pepper Seed Oils Harvested from the Chungsong and Youngyang Areas (청송.영양산 고추씨 기름의 Triglyceride 조성의 비교)

  • 김동수;전선미;정동윤;김현대;박영호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.3
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    • pp.225-232
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    • 1991
  • A Study was carried out to elucidate the triglyceride compositions of the red pepper seed oils harvested from two different areas. The oil was extracted from the red pepper seed with nhexane. Each triglyceride of the oil was separated by thin layer chromatography (TLC) and fractonated by reverse phase high performance liquid chromatography (HPLC) on the basis of acyl carbon numbers, and partition number group(PN) and fatty acid composition of triglyceride were analyzed by gas liquid chromatography (GLC). From the results, it was found that the red pepper seed oils of the Chungsong and Youngyang areas consisted of 14 and 18 kinds of triglycerides, respectively. The red pepper seed oil of the Chungsong area consisted of (C18:2, C18:2, C18:2=41.0%), (C16:0, C18:2, C18:2=37.1%), and that of the Youngyang area consisted of (C18:2, C18:2, C18:2=41.0%), (C16:0, C18:2, C18:2=36.3%) and (C16:0, C16:2, C18:2=8.4%), as the major triglycerides.

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Seed Germination Study to Determine the Feasibility of Local Resources as Materials for Ecological Restoration (생태복원용 소재로서 현지자원의 이용가능성 파악을 위한 발아실험 연구)

  • Kim, Kyung-Hoon;Kim, Young;Joo, Paik;Hur, Young-Jin
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.18 no.1
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    • pp.153-163
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    • 2015
  • The study was conducted to develop ecological restoration method of damaged area in DMZ vicinities. As the material for the ecological restoration, forest soil and trees waste have been used. Forest soils were collected for experiments in Yanggu, Gangwon Province. Effect of ecological restoration was analyzed through germination experiment. Germination experiment was performed using 12 kinds of woody and herbaceous seeds. Woody and herbaceous seed germination in test pot was relatively evenly. Mixed material consisting of forest soil and trees waste seemed a possibility as the material for the restoration. The effects on seed germination were higher in the case of mixing more than 70% by volume ratio of local resources. Total number germinated individuals were different depending on the mixing ratio of soil sampling depth. Individual plants showed different trends depending on the experimental combination. Results of the woody seed germination were affected only in the soil sampling depth. Seed germination of herbaceous received a combined effect on soil sampling depth and mixing ratio.

Studies on the Combining Ability of Major Agronomic Characters in Pea (완두의 주요 형질의 조합능력에 관한 연구)

  • Chung, Hae-Jin;Chang, Kwon-Yawl
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.3
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    • pp.250-256
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    • 1992
  • This study was carried out to obtain basic genetic informations in pea(Pisum sativum L.). A series of field experiments were carried out with seven pea cultivars and F$_1$ hybrids for three years. Based on the information collected from a set of diallel crosses, the behaviours of inheritance of seed yield and ten major agronomical characters were observed by the estimation of heterosis and combining ability. Heterosis for seed yield per plant was 79% and those for yield components such as number of seeds per plant, number of pods per plant, stem length, stem diameter, number of branches per plant, also showed relatively high values. General combining ability(GCA) and specific combining ability(SCA) were significantly different among major agronomic characters, and the variances of GCA were higher than those of SCA latter. Among the tested cultivars, Alderman was found to be the most promising parent based upon the GCA effects on the seed yield and yield components. The crosses of Sugar Ann$\times$Alderman, Wando$\times$Alderman and JI 1198$\times$Chinju #6, showed the highest SCA effects on seed yield and yield components.

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Relationship between Seed Weight and Agronomic Traits in Korean Native Soybeans (재래종 대두의 종실중과 저형질과의 관계)

  • Song, Hi-Sup;Lee, Young-Il;Kwon, Shin-Han;Song, In-Man;Kwon, Kyeong-Hak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.28-33
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    • 1990
  • The present study was conducted to evaluate agronomic characters of the soybean germplasm which was collected from the sites covering southern parts of Korean peninsula. The total collections of 2748 native soybean varieties were grouped by eight levels of seed weight and the correlation between the seed weight and the other agronomic traits were determined. Seed weight was ranged from 7. 3g to 48. 4g with a great variation and averaged 23. 5g. Seed size of the many local lines were relatively large than that of foreign varieties. Frequency distribution of the germplasm for seed weight were 2.2% in Group 1 (below 10.0g), 14.7% in Group 2 (10.1-15.0g), 11.7% in Group 3 (15.1-20.0g), 26.2% in Group 4 (20.1-25.0g), 30.2% in Group 5 (25.1-30.0g). 10.6% in Group 6 (30.1-35.0g), 3.3% in Group 7 (35.1-40.0g) and 1.1% in Group 8 (over 40.0g). There was positive correlationship between seed weight and maturity or fruiting period, while there was negative correlationship between the seed weight and the other traits such as days to flowering, plant height, and number of nodes and pods. While 100 seed weight of 464 lines (16.9% of total lines) were below 15g, and those are adequate for the bean sprouts. The fruiting period of large seeded lines was longer, however the number of pods per plant of those were less lines than that of small seeded lines.

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Optimum Sowing Date for Seed Production in Hemp (대마의 채종재배를 위한 파종적기)

  • 박규철;박태동;박인진;권병선;김상철;정병준;김명석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.424-428
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    • 1997
  • This study was carried out to investigate the effects of sowing dates, Apr. 25, May 15, June 5 and June 25, on sex ratio, growth and seed yield of "Chonnam species", local variety of hemp plant. The results were following as. : In sex ratio according to the different seeding dates, the revealating rate of female and male plants were highest on June 25, and Apr. 25, respectively. The length of stem, the number of branches, nodes, and seed yield were increased on May 15, but these charateristics were rapidly reduced on June 25 because of short growing period. Yield of seeds was increased by 24% on May 15 comparing with 1.30ton /ha on Apr. 25.

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Ultrastructural Changes and Formation of Storage Materials in Endosperm Cells during the Seed Formation of Panax ginseng C.A. Meyer (인삼(Panax ginseng C.A. Meyer)의 종자형성에 있어서 배유세포의 미세구조의 변화 및 저장물질의 형성)

  • 유성철
    • Journal of Plant Biology
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    • v.34 no.3
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    • pp.201-213
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    • 1991
  • This study has been carried out to investigate the ultrastructural changes, formation of storage materials in endosperm cells with electron microscope during the seed formation of Panax ginseng C.A. Meyer. In the early stage of seed formation with green seed coat, the endosperm was cellular type. Cell plate was largely composed of dictyosome vesicles in early stage of wall formation after mitosis. Central vacuole was gradually subdivided into several small-sized vacuoles. During the differentiation of plastids, some proplastid was replaced by amyloplast with starch grains and lamellar structure. A number of mitochondria with well developed cristae were distributed in cytoplasm. Rough endoplasmc reticulum, dictyosome, microbody, free ribosomes and polysomes were evenly distributed in cytoplasm. Spherical spherosomes were formed from dictyosome containing the lipid materials of even electron density. Protein bodies were formed by interfusing between vacuoles and vesicles derived from rough endoplasmic reticulum which contained the amorphous protein of high electron density.

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