• Korean Journal of Environmental Agriculture
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• v.23 no.1
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• pp.1-6
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• 2004

The seeding sources and concentration of ammonia on anaerobic digestion were investigated by batch culture bioreactors. The sources of seeding on anaerobic digestion were from swine wastewater collection pit of a hog raising farm and from anaerobic digestion sludge of a municipal sewage treatment plant. The inhibition of ammonia on anaerobic microorganisms was initiated at ammonia concentration of $1,500\;mgNH_4-N/L$ and it's effect was increased by increased by increasing ammonia concentration up to $3,500\;mgNH_4-N/L$ regardless the sources of seeding as evidenced by decreases in COD removal efficiencies and biogas yields. The inhibition occurred to not only methanogens but also acidogens since the concentration of volatile fatty acids was maintained at 50 mg/L The COD removal efficiency and biogas yield were Maintained constantly while increasing ammonia concentration up to $3,500\;mgNH_4-N/L$ when swine wastewater collection pit was used as a seeding; however, those were decreased while increasing ammonia concentration when anaerobic digestion sludge was used as a seeding. The results indicate that the seeding acclimated to high concentrations of ammonia for long time was easy in adaptation to high ammonia concentration and less subjective to ammonia inhibitory effects.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.381-387
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• 2010

This study was performed to investigate differences of antioxidant activity, the content of free amino acids including GABA(gama-amino butyric acid) among mungbean cultivars grown in Highland area (Jinbu) and to compare those between seeds and sprouts. In DPPH (diphenyl picrydrazyl) radical scavenging activity, 'Jangan' mungbean activity was more higher than that of other cultivars. The content of total phenolic compounds of mungbean ranged from 1,186 to $1,493\;{\mu}g/g$ in seed and 2,321 to $2,783\;{\mu}g/g$ in sprout. Among amino acids of seeds, the content of glutamic acid was highest in 'Soseon' mungbean, and contents of almost amino acids increased during sprouting of seeds. The content of GABA that are known as a material having high functional effects on human body was also analyzed. When the relatively high content of GABA was observed in seeds of almost mungbean grown in highland, and their contents rapidly increased after sprouting from seeds. As a result of this experiment, it is expected that mungbean will be developed to a good alternative crop having high value as functional food materials in highland area of Korea.

• Horticultural Science & Technology
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• v.18 no.4
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• pp.508-512
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• 2000

Growth of red pepper (Capsicum annuum) 'Kumtap', tomato (Lycoperisocon esculentum) 'Seokwang', petunia (Petunia hybrida) 'Madness Rose', and pansy (Viola tricolor) 'Magestic Giant' in mixtures of chestnut woodchips and granular rockwool at 25:75, 50:50, or 75:25 (v/v) was examined. Chestnut woodchips were soaked in water for 48 hours or aged in open field for 6 months in order to remove substances impeding plant growth. A commercial plug medium was used as the control. All treatments showed in a similar result in red pepper, petunia, and pansy. Plant height, fresh weight, dry weight, and chlorophyll content in media containing chestnut woodchips, especially in higher proportions, were poorer as compared to those in the control. On the contrary, height, fresh weight, and dry weight of tomato seedlings in media containing woodchips were significantly higher than those in the control. For petunia, pansy, and red pepper, six month ageing in open field of woodchips was more favorable for growth than 48 hour water soaking. Emergance of petunia seed was inferior, especially when woodchip content was higher, to the other crops with a resultant growth suppression. From the results, chestnut woodchips proved to be a practical material as a medium component only in tomato plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.189-196
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• 1999

This study was conducted to evaluate the dry matter yield, quality and utilization of nutrients of wild Korean lespedeza as a forage legume sources. Dry matter(DM) yield of stages, chemical composition and DM digestibility were observed, also DM intake, digestibility and utilization of nitrogen and energy by Korean native goats were determined. Experimental diets include three treatrnents ; mixture grass hay(GS), GS 80% + lespedeza(LA) 20% and GS 60% + LA 40%, respectively. The DM yield of Korean lespedeza(LA) with advancing the growing stage tended to significantly increase(P<0.05). The average DM yield was observed 7,336kg/ha. CP content of LA tended to decrease, but fiber content tended to increase at growing stage advanced(P<0.05). The tannin content of LA was highest in bud stage (54.5mg/g), but tended to decrease at growing stage advanced, lowest in seed ripe stage(26.8mg/g) (P<0.05). With increasing the level of LA supplementation, DM intake by Korean native goats slightly increased, but no differences were observed in all diets. The digestibility of DM, cellular constituents and NDF were slightly lower for GS 100% diets than LA 20% and 40% supplemented diets(P<0.05). Apparently digested N% was not detected significant differences in all diets, but retained N and biological value were higher for LA supplemented diets than for GS 100% diets (P<0.05). Utilization of DE and ME was not detected significant differences in all diets. Above the results, it could be suggested that LA have a potential to provide as forage legume sources considering the DM yield, DM digestibility and nitrogen utilization.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.499-505
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• 1987

The functional properties of lupin seed protein concentrate (LPC) were examined and compared to those of soybean protein isolate (SPI) and Na-caseinate. LPC-50, of which protein level was 50%, was prepared by a two phase solvent (hexane: alcohol: water= 10:7:3) extraction method. LPC-70 was made from LPC-50 by removing the fractions solubilized by carbohydrate decomposing enzymes. The solubilities of LPC-50 and LPC-70 were similar to that of of SPI but slightly higher at pH 4-5, and less susceptible to the added salt. The apparent viscosity of LPC increased exponentially as the concentration increased over 6% level, and the change was similar to that of Na-caseinate. LPC showed strong pseudoplastic non-Newtonean flow behavior, which was similar to that of SPI The emulsifying capasity of LPC-70 was similar to that of SPI when salt was added. The foaming capacity of LPC was comparable to that of SPI. LPC showed high oil and water absorption capacities, which increased as the protein level was elevated. LPC-70 showed the highest oil absorption capacity of all the samples tested.

• Food Science and Preservation
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• v.20 no.3
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• pp.342-347
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• 2013

In recent years, polyphenol-rich herbs, fruits and processed foods, which are made of plant origin, have attracted much attention due to their potential health benefits. Pomegranate (Punica granatum L.) is an important source of bioactive compounds and has been used to treat diseases because of its medicinal properties. This research was focused on characterizing Korea's national cultivar and a similar product from California, USA. To evaluate their bioactive compounds and pharmacological activities, their anti-oxidation and cancer inhibition properties, as well as their organic acid and free sugar contents, were investigated. The national cultivar had low total sugar and high organic acid contents, contrary to the imported product. The results showed that the peel of national cultivar had high polyphenol and ellagic acid contents compared to imported product. The free radical scavenging capacity was evaluated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and its positive correlation with the total polyphenol contents was found. The anti-cancer activity of methanol extracts revealed growth inhibition against the prostate cancer cell. These results signify that while pomegranate, national cultivar, is more sour than the imported product, its health benefits could be excellent. Also, the polyphenol compound content of the non-edible part (such as the peel and the seed) was higher than that of the juice. Thus, it is suggested that the byproduct of the juice extraction could be potentially used in other fields such as medicine or dietary agents.

• Applied Biological Chemistry
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• v.18 no.2
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• pp.65-70
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• 1975

The cultivation of ginseng plant (Panax ginseng C.A. Meyer) in Korea as an eminent medicinal herb may be traced far back in history. However, the practices in cultivation have not much improved in terms of efficiency and scientific farming. In the present study some experiments were undertaken for the search of the soil and nutrition conditions, because of the nutritional requirement of ginseng plant shaws quite unique compared with other crops. In both the seed bed and the field 'Yakto' has been traditionally employed or the prime source of nutrition of the crop. Yakto is a complex matter prepared from raw foliage of the broad-leaved trees as the main portion with the admixture of a variety of organic nitrogen source through fermentative processes. The composition of Yakto may be classified coarsely into the decomposed and undecomposed substances, the former being further fractionated according their solubilities, comprising also various colloidal matters whose composition and structure are yet to be known. The Yakto-fractions were subjected to analyze for search of its nature and coarse composition in terms of the distribution of nitrogen, contents of organic functional groups such as -COOH, phenolic-OH, alcholic-OH and methoxyl and hydrolysable sugars. Furthermore, absorption-spectra of each fraction were determined in visible and infrared region and compared the results each other.

• The Korean Journal of Pesticide Science
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• v.3 no.2
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• pp.19-28
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• 1999

To investigate the physiological and biochemical differences between susceptible and tolerant species of Echinochloa species to the foliar applied cyhalofop-butyl and pyribenzoxim herbicides, herbicidal response, the rate of absorption, translocation and metabolism were studied. Among 148 individuals of Echinochloa species collected from 41 sampling sites in Korea which were classified into 7 geographical regions, based on morphological characteristics of plant and seed type, 46 lines were used for further studies. From them, E. crus-galli var. praticola collected from Hwanggan and E. crus-galli var. crus-galli collected from Namyangju were selected as the most susceptible species to cyhalofop-butyl and pyribenzoxim, respectively. Meanwhile, E. oryzicola(from Cheju) and E. crus-galli var. crus-galli(from Asan) were selected as tolerant species to cyhalofop-butyl and pyribenzoxim, respectively. Application of radio-labelled herbicides on the 1st leaf to both susceptible and tolerant Echinochloa species exhibited that the applied $^{14}C$-Cyhalofop-butyl and $^{14}C$-pyribenzoxim were more easily absorbed and translocated into stem tissues than root. Absorption of $^{14}C$-Cyhalofop-butyl increased rapidly at 1 h after application and reached the maximum at 12 h after application. However, the absorption rate of $^{14}C$-pyribenzoxim was not changed. Two metabolites from cyhalofop-butyl-treated plants and one metabolite from pyribenzoxim-treated plants were separated by TLC. The amount of metabolite 1 in cyhalofop-butyl-treated tolerant species was significantly higher than that in the susceptible one, suggesting differences in detoxification ability between susceptible and tolerant species.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.05a
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• pp.35-38
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• 2004

1950-1960 년대의 녹색혁명은 1970년 Nobel 평화상수 상자 Norman Borlaug가 주도했었다. 제1 녹색혁명은 지구상의 가장 큰 문제점중의 하나인 기아를 해결하는지 크게 일조하였다. 많은 사람들을 굶주림으로부터 해방시켰지만 굶주리는 사람보다 더 많은 사람들이 매일 태어나고 있고, 지구의 인구증가는 지금까지 계속되고 있다. 언제까지 인구증가가 계속될 것인지, 인구증가에 따른 식량자원의 증가도 비례할 것인지는 오래전부터 인류의 관심의 대상이 되어 왔고, 자연스럽게 지구의 기아를 해결하는 제2의 녹색혁명은 과학자들의 연구를 집중시키는 결과를 낳게 되었다. 1980 년대에 미국 Monsanto 회사에서 유전공학적으로 개발한 Roundup-Ready 제초제저항성 및 Bt-살충 농작물을 선보이면서 제2의 녹색혁명이 시작되었다고 볼 수 있겠다. 이렇게 제2의 녹색혁명은 유전공학에 의한 CMO 식물$\cdot$작물에 의해 시작되었다. Monsanto 회사는 살충제 RoundUp 제초제 저항성 옥수수, 콩, 목화, Canola (유채꽃, rape seed) 등을 개발하여 이미 상업화하였다. 1960년대 쌀의 녹색혁명도 유전적으로 벼집이 짧은 품종에 의해 이루어졌다. 최근에 개발된 GMO "Golden Rice"는 비타민 A와 철분의 함량을 대폭 증대 시켜 세계 영양결핍 아동들의 건강과 시각을 향상시킬 것으로 기대되는데, "Golden Rice"는 제1회 금호국제과학상 수상자인 Potrykus (스위스공대) 교수가 개발하였다. 그러나 현재까지는 그 보급에 많은 장애물이 있다. 특히 GMO의 환경과 건강에 대한 안전성을 의심하는 사람들이 많다. 제2의 녹색혁명의 또 다른 분야는 식물의약 분야이다. GMO 개발에 적용되는 기술을 이용하면 taxol 같은 항암제, carotene 같은 항산화 영양제 등의 대량 생산이 가능해진다. 식물은 화학적 합성이 아주 까다로운 약제물질 등을 천연상태에서 합성하고 있기 때문이다. 또 식물은 lipoxygenase 효소계가 있어서 마치 천연물 석유제조공장과 같은 제조공정 capacity를 가지고 있다. 그러면 식물/식품 GMO는 안전한 것인가? 아니, KBS의 한 사회자가 말했듯이, 그리고 많은 소비자들이 믿는 것처럼 GMO는 위험한가? GMO에 대한 일반 사람들의 공포감은 Green Peace 당원들뿐만 아니라 일부 과학자들에 의해서도 조장되고 있다. 이러한 분위기 속에서 GMO에 의한 제2 녹색혁명은 Africa 대륙에서의 제1 녹색혁명이 지금도 지연되는 것과 같다고도 볼 수 있다. GMO의 환경에 대한 악영향은 과대 선전되어있는 것이 아닌가? 마치 GMO가 화학비료, 농약제보다 더 위험하다고 믿는 사람들도 많다. 나는 이러한 GMO 공포증이 과학적으로 그리고 "Risk Assessment"의 견지에서 볼 때 그 근거가 희박하다고 보여주는 몇 몇 실험 및 경험 사실들을 인용하려 한다. 그리고 올바른 Risk Assessment야 말로 한국의 21세기 BT 산업을 경쟁력 있게 하고 국민 년 소득 2만불 달성에 중요한 기여를 하게 될 것이라고 생각한다. 한국은 농토가 적고 천연자원이 빈약하다. GMO는 21세기의 생존 경쟁 산업이다. 제2의 녹색혁명은 얼마든지 가능하며, 한국은 부족한 농토와 빈약한 자원에도 불구하고 능력 있는 인적자원이 풍부하여 GMO 개발 연구에 국제적 경쟁력을 키울 수 있다. 그러나 GMO에 대한 논쟁만 하고 있으면 이미 때가 늦는다. 미국은 이미 GMO-BT 시장을 거의 완전 독점했으며, 타국에서의 논쟁과 불합리적으로 엄격한 GMO 관련 규정을 조장하고 환영한다.

• KSBB Journal
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• v.16 no.4
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• pp.344-350
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• 2001

The effect of salinity stress of Brassica olearacea and Capsicum annuum were studied at various levels of salinity conditions(Na-gluconate, K-gluconate, NaCl, KCl). The effects of salinity stress were measured by seedling growth rates and secondary metabolites contents of the stressed plants. Each seedling studied on the response of different salinity stress. Seedling growth of Capsicum annuum was inhibited up to 200 mM salt tolerance and Brassica olearacea was inhibited up to 400 mM salt tolerance. The produced anthocyanin was separated to high value from 200 mM NaCl in case of Brassica olearana and 50 mM K-gluconate in case of Capsicum annuum. The BADH activity was very high in Brassica olearacea seedlings treated with 200 mM NaCl and in Capsicum annuum seedlings treated with 100 mM K-gluconate. The BADH activities were increased during the early culture days, it induced betaine synthesis. The salinity stress promoted BADH activiy, subsequently endogenous betaine contents were increased, and it seemed to be secure seedling from salinity stress. The salinity concentration of 200 mM was effective on the inhibition of seed germination and on the increase of proline accumulation in tissue. The inhibition of seedling growth and accumulation of secondary metabolites in seedling were caused osmotic hypersensitivity against salinity stress.