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Application of AFLPs to Phylogenetic Analysis of Aegilops (AFLPs에 의한 Aegilops의 계통발생학적 재평가)

  • Park, Yong-Jin;Shim, Jae-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.790-799
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    • 1997
  • Aegilops genus is known to include the donor species of the Band D genome of the bread wheat(ABD). An effort to establish a better strategy for phylogenetic relationships about Aegilops polyploids by AFLPs(Amplified Fragment Length Polymorphisms) was conducted using the 19 Aegilops sPP. and T. aestivum. The 207 polymorphic bands from the amplified products on the 6% acrylamide denaturing sequencing gels were obtained with the 7 AFLP primer combinations, and used to account for the genetic similarities and cluster analysis using NTSYS program. According to the genome analysis, the $M^h$-genome of Ae. heldreichii was estimated as an intermediate genome between the M-genome of Ae. comosa and N-genome of Ae. uniaristata and supposed to be incorporated in the establishing process of UM-genome as a possible diploid donor. And Ae. ventricosa(DN) was more close to Ae. umbellulata(U) than Ae. squarrosa(D). The close relationship between Ae. squarrosa and T. aestivum was perceived as a diploid donor of D-genome. As for the polyploid species, hexaploid Ae. triaristata was more closely related to Ae. columnaris rather than tetraploid Ae. triaristata. The clustered groups were, basically same to the previous Gihara's sections based on phenotypes and pairing analysis of interspecific hybrids. AFLP was evaluated as an efficient and powerful method in the genome evaluation of closely related species.

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Seismic damage evaluation of steel reinforced recycled concrete filled circular steel tube composite columns

  • Hui, Ma;Xiyang, Liu;Yunchong, Chen;Yanli, Zhao
    • Earthquakes and Structures
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    • v.23 no.5
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    • pp.445-462
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    • 2022
  • To investigate and evaluate the seismic damage behaviors of steel reinforced recycled concrete (SRRC) filled circular steel tube composite columns, in this study, the cyclic loading tests of 11 composite columns was carried out by using the load-displacement joint control method. The seismic damage process, hysteretic curves and performance indexes of composite columns were observed and obtained. The effects of replacement rates of recycled coarse aggregate (RCA), diameter thickness ratio, axial compression ratio, profile steel ratio and section form of profile steel on the seismic damage behaviors of composite columns were also analyzed in detail. The results show that the failure model of columns is a typical bending failure under the combined action of horizontal loads and vertical loads, and the columns have good energy dissipation capacity and ductility. In addition, the replacement rates of RCA have a certain adverse effect on the seismic bearing capacity, energy consumption and ductility of columns. The seismic damage characteristics of composite columns are revealed according to the failure modes and hysteretic curves. A modified Park-Ang seismic damage model based on the maximum displacement and cumulative energy consumption was proposed, which can consider the adverse effect of RAC on the seismic damage of columns. On this basis, the performance levels of composite columns are divided into five categories, The interlayer displacement angle and damage index are used as the damage quantitative indicators of composite columns, and the displacement angle limits of composite columns at different performance levels under 80% assurance rate are calculated as 1/105, 1/85, 1/65, 1/28, and 1/25 respectively. On this basis, the damage index limits corresponding to each performance level are calculated as 0.045, 0.1, 0.48, 0.8, and 1.0 respectively. Finally, the corresponding relations among the performance levels, damage degrees, interlayer displacement angles and damage indexes of composite columns are established. The conclusions can provide reference for the seismic design of SRRC filled circular steel tube composite columns, it fills the vacancy in the research on seismic damage of steel reinforced recycled concrete (SRRC) filled circular steel tube composite columns.

Design and Structural Safety Evaluation of 1MW Class Tidal Current Turbine Blade applied Composite Materials (복합재료를 적용한 1MW급 조류 발전 터빈 블레이드의 설계와 구조 안전성 평가)

  • Haechang Jeong;Min-seon Choi;Changjo Yang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.7
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    • pp.1222-1230
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    • 2022
  • The rotor blade is an important component of a tidal stream turbine and is affected by a large thrust force and load due to the high density of seawater. Therefore, the performance must be secured through the geometrical and structural design of the blade and the blade structural safety to which the composite material is applied. In this study, a 1 MW class large turbine blade was designed using the blade element momentum (BEM) theory. GFRP is a fiber-reinforced plastic used for turbine blade materials. A sandwich structure was applied with CFRP to lay-up the blade cross-section. In addition, to evaluate structural safety according to flow variations, static load analysis within the linear elasticity range was performed using the fluid-structure interactive (FSI) method. Structural safety was evaluated by analyzing tip deflection, strain, and failure index of the blade due to bending moment. As a result, Model-B was able to reduce blade tip deflection and weight. In addition, safety could be secured by indicating that the failure index, inverse reserve factor (IRF), was 1 or less in all load ranges excluding 3.0*Vr of Model-A. In the future, structural safety will be evaluated by applying various failure theories and redesigning the laminated pattern as well as the change of blade material.

Melatonin Attenuates Mitochondrial Damage in Aristolochic Acid-Induced Acute Kidney Injury

  • Jian Sun;Jinjin Pan;Qinlong Liu;Jizhong Cheng;Qing Tang;Yuke Ji;Ke Cheng;Rui wang;Liang Liu;Dingyou Wang;Na Wu;Xu Zheng;Junxia Li;Xueyan Zhang;Zhilong Zhu;Yanchun Ding;Feng Zheng;Jia Li;Ying Zhang;Yuhui Yuan
    • Biomolecules & Therapeutics
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    • v.31 no.1
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    • pp.97-107
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    • 2023
  • Aristolochic acid (AA), extracted from Aristolochiaceae plants, plays an essential role in traditional herbal medicines and is used for different diseases. However, AA has been found to be nephrotoxic and is known to cause aristolochic acid nephropathy (AAN). AA-induced acute kidney injury (AKI) is a syndrome in AAN with a high morbidity that manifests mitochondrial damage as a key part of its pathological progression. Melatonin primarily serves as a mitochondria-targeted antioxidant. However, its mitochondrial protective role in AA-induced AKI is barely reported. In this study, mice were administrated 2.5 mg/kg AA to induce AKI. Melatonin reduced the increase in Upro and Scr and attenuated the necrosis and atrophy of renal proximal tubules in mice exposed to AA. Melatonin suppressed ROS generation, MDA levels and iNOS expression and increased SOD activities in vivo and in vitro. Intriguingly, the in vivo study revealed that melatonin decreased mitochondrial fragmentation in renal proximal tubular cells and increased ATP levels in kidney tissues in response to AA. In vitro, melatonin restored the mitochondrial membrane potential (MMP) in NRK-52E and HK-2 cells and led to an elevation in ATP levels. Confocal immunofluorescence data showed that puncta containing Mito-tracker and GFP-LC3A/B were reduced, thereby impeding the mitophagy of tubular epithelial cells. Furthermore, melatonin decreased LC3A/B-II expression and increased p62 expression. The apoptosis of tubular epithelial cells induced by AA was decreased. Therefore, our findings revealed that melatonin could prevent AA-induced AKI by attenuating mitochondrial damage, which may provide a potential therapeutic method for renal AA toxicity.

A Case Study on the Cause Analysis of Land creep Using Geophysical Exploration (물리탐사를 활용한 땅밀림 원인분석의 사례적 연구)

  • Jae Hyeon Park;Gyeong Mi Tak;Kook Mook Leem
    • Journal of Korean Society of Forest Science
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    • v.112 no.3
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    • pp.382-392
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    • 2023
  • Recent reports have indicated a rapid increase in the frequency of sediment disasters due to climate change and other changes in the geological environment. Given this alarming situation and the recent increase in the frequency of land creep in Korea, systematic and efficient recovery and management of land creep areas is essential. The purpose of this study is to identify disaster vulnerability by conducting a physical exploration of land creep in San 4-1, Jayeon-ri, Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, and examine stability by identifying the overall geological structure of the affected ground. In addition, drilling surveys are conducted to verify the reliability of the measured data. The results of the study reveal that low specific resistance abnormalities are distributed in the upper part of the soil layer and weathering zone and that this section is a 50-120 m exploration line. It is also confirmed to be a low-hardness ground area where tensile cracks are observed. Therefore, there is a need for research focused on developing measures to reduce economic and social damage within the domestic context by continuously monitoring indicators of land creep and identifying land creep risks.

Instrumentation Management of Differential Settlement of the Deep Soft Ground with Dredged Clay Reclaimed in the Upper (대심도 준설 매립지반에서의 층별침하 계측관리에 관한 사례 연구)

  • Tae-Hyung Kim;Seung-Chan Kang;Ji-Gun Chang;Soung-Hun Heo
    • Journal of the Korean Geosynthetics Society
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    • v.22 no.1
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    • pp.87-96
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    • 2023
  • There are a lot of difference between the surface settlement and the differential settlement measured at the Busan New Port, where the dredged and reclaimed clay layer exists and below the clay is originally thickly distributed. To find the cause and solution of this, the actual conditions of each differential settlement used for the soft ground improvement, characteristics, installation method, measurement frequency, measurement data management, and data analysis of each type were considered. In the deep soft ground improvement work where large deformation occurs, the bending deformation of the screw-type differential settlement gauge is less than that of other types of measuring instruments, so there is less risk of loss, and the reliability of data is relatively high as the instruments are installed by drilling for each stratum. Since the greater the amount of high-precision settlement measurement data, the higher the settlement analysis precision. It is necessary to manage with higher criteria than the measurement frequency suggested in the standard specification. For the data management of the differential settlement gauge, it is desirable to create graphs of the settlement and embankment height of the relevant section over time, such as surface, differential, and settlement of pore water pressure gauge for each point. In the case of multi-layered ground with different compression characteristics, it is more appropriate to perform settlement analysis by calculating the consolidation characteristics of each stratum using a differential settlement data.

Evaluation of Traffic Vibration Effect for Utilization of Abandoned Mine Openings (휴·폐광산 채굴 공동 활용을 위한 교통 진동 영향 평가)

  • Hyeon-Woo Lee;Seung-Joong Lee;Sung-Oong Choi
    • Tunnel and Underground Space
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    • v.33 no.2
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    • pp.95-107
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    • 2023
  • In this study, the effect of repeated traffic vibration on the long-term stability of mine openings is analyzed for re-utilization of abandoned mine galleries. The research mine in this study is an underground limestone mine which is developed by room-and-pillar mining method, and a dynamic numerical analysis is performed assuming that the research mine will be utilized as a logistics warehouse. The actual traffic vibration generated by the mining vehicles is measured directly, and its waveform is used as input data for dynamic numerical analysis, As a results of dynamic numerical analysis, after 20,000 repetitions of traffic vibration, the mine openings is analyzed to be stable, but an increase in the maximum principal stress and an additional area of plastic zone are observed in the analysis section. As shown in the changes of displacement, volumetric strain, and maximum principal stress which are measured at the mine opening walls. It is confirmed that if the repeated traffic vibration is continuously applied, the instability of the mine openings can be increased. Authors expect that the results of this study can be used as a reference for basic study on utilization of abandoned mine.

Evaluation of Consolidation Characteristics Considering the Mixed Gradation Ratio of Soft Ground (연약지반의 입도 혼합비를 고려한 압밀특성평가)

  • Park, Yeong-Mog;Yun, Sang-Jong;Chea, Jong-Gil
    • Journal of the Korean Geotechnical Society
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    • v.25 no.10
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    • pp.99-110
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    • 2009
  • In order to provide the design criteria, the characteristics of consolidation for soft ground improvement have been investigated using the field banking test performed by the vertical drain method at the northern container section in Busan New Port. Field test results indicated that the estimated degree of consolidation in design stage decreased by about 7% compared with the measured one. This difference is attributed to the fact that the conservative geological properties were applied with relatively high amount of maximum clay mixture ratio during the design stage. Based on this findings, another laboratory oedometer test was implemented to consider various combination of mixture ratio. It was found that the consolidation degree increased in accordance with the increase of sand/silt mixture ratio. Also, the proportion of 10%, 50%, and 40% for sand, silt, and clay, respectively, was observed as the best combination of mixture ratio to the actual measurement, which is very similar to the average grain size distribution in the banking test area. Therefore, it is suggested that the overall geological characteristics as well as the grain size distribution should be considered in design stage to improve the soft ground that contains mixture of sand, silt, and clay.

Calculation of Crack Width of the Top Flange of PSC Box Girder Bridge Considering Restraint Drying Shrinkage (구속 건조수축을 고려한 PSC BOX 거더교 상부플랜지 균열폭 산정)

  • Young-Ho Ku;Sang-Mook Han
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.27 no.3
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    • pp.30-37
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    • 2023
  • The PSCB girder bridge is a closed cross-section in which the top and bottom flanges and the web are integrated, and the structural characteristics are generally different from the bridges in which the girder and the floor plate are separated, so a maintenance plan that reflects the characteristics of the PSCB girder bridge is required. As a result of analyzing damage types by collecting detailed safety diagnosis reports of highway PSCB girder bridges, most of the deterioration and damage occurring during use is concentrated on the top flange. In particular, cracks in the bridge direction on the underside of the top flange occurred in about 70 % of the PSCB girder bridges to be analyzed, and these cracks were judged to be caused by indirect loads such as heat of hydration and drying shrinkage rather than structural cracks caused by external loads. In order to improve durability and reduce maintenance costs of PSCB girder bridges in use, it is necessary to control restraint drying shrinkage cracks from the design stage. Therefore, in this paper, the cracks caused by drying shrinkage under restraint, which is the main cause of cracks under the flanges of the top part of the PSCB girder bridge, were directly calculated using the Gilbert Model, and the influencing factors such as the amount of reinforcing bars, diameter and spacing of reinforcing bars were analyzed. As a result of the analysis, it was found that the crack width caused by restraint drying shrinkage exceeded the allowable crack width of 0.2 mm for reinforcing bars with a reinforcing bar ratio of 0.01 or less based on the H16 reinforcing bar and a reinforcing bar with a diameter greater than H19 based on the reinforcing bar ratio of 0.01. Finally, based on the results of the crack width review, a method for controlling the crack width of the top flange of the PSCB girder bridge was proposed.

The Political Recognition Surrounding Candlelight Rally and Taegeukgi Rally: A Big Data Analytics on Online News Comments (촛불 집회와 태극기 집회를 둘러싼 정국 인식: 온라인 뉴스 댓글에 대한 빅데이터 분석)

  • Kim, ChanWoo;Jung, Byungkee
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.8 no.6
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    • pp.875-885
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    • 2018
  • This study analyzed the major issues of the Candlelight Rally and Taegukgi Rally registered in news comments of the politics section of the portal site from October 24, 2016 to March 19, 2017. We examined the political recognition of the two rallies with the Named Entity Recognition. The main analytical items are the responsibility for impeachment, the subject and method of settlement, and other major issues. As a result of the analysis, the comments of the Candlelight Rally focused on the impeachment support and the legal penalties of the regime ministers, and insisted on resolving the political situation through the next election after impeachment. The comments of the Taegukgi Rally focused on the rejection of the impeachment to maintain the regime and insisted on rejecting the impeachment of the Constitutional Court. The conflicts between the group that supported Candlelight Rallis and the group that supported Taegukgi rallies are predicted to last at least for the time being (Park Geun-hye's trial period) after the presidential election. After the impeachment of the President and replacement of the regime this conflict will develop into the confrontation between the pursuit of liquidation and new politics and the attempt to influence the trial of Park Geun-hye. Therefore, the efforts to integrate society in the aftermath are necessary.