• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.164-166
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• 2001

To examine the characteristics of the recombinant thin aggregative fimbriae of Salmonella, the AgfA subunit gene was amplified from Salmonella enteritidis using a PCR. The maltose binding protein (MBP)-AgfA fusion protein was overproduced in E. coli and purified. The secondary structure of AgfA was then elucidated from the difference CD spectra. An estimation of the secondary structure of AgfA using the self-consistent method revealed a mostly ${\beta}-sheet$ structure.

• Earthquakes and Structures
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• v.16 no.4
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• pp.401-413
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• 2019

The concept of base isolation for equipment is well known. Its application in buildings and structures is rather challenging. Introduction of horizontal flexibility at the base helps in proper energy dissipation at the base level thus reducing the seismic demand of the super structure to be considered during design. The present study shows the results of a series of numerical simulation studies on seismic responses of secondary system (SS) housed in non-isolated and base-isolated primary structures (PS) including equipment-structure interactions. For this study the primary structure consists of two similar single bay three-store reinforced cement concrete (RCC) Frame building, one non-isolated with conventional foundation and another base isolated with Lead plug bearings (LPB) constructed at IIT Guwahati, while the secondary system is modeled as a steel frame. Time period of the base isolated building is higher than the fixed building. Due to the presence of isolator, Acceleration response is significantly reduced in both (X and Y) direction of Building. It have been found that when compared to fixed base building, the base isolated building gives better performance in high seismic prone areas.

• Genomics & Informatics
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• v.11 no.3
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• pp.155-160
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• 2013

Structural information has been a major concern for biological and pharmaceutical studies for its intimate relationship to the function of a protein. Three-dimensional representation of the positions of protein atoms is utilized among many structural information repositories that have been published. The reliability of the torsional system, which represents the native processes of structural change in the structural analysis, was partially proven with previous structural alignment studies. Here, a web server providing structural information and analysis based on the backbone torsional representation of a protein structure is newly introduced. The web server offers functions of secondary structure database search, secondary structure calculation, and pair-wise protein structure comparison, based on a backbone torsion angle representation system. Application of the implementation in pair-wise structural alignment showed highly accurate results. The information derived from this web server might be further utilized in the field of ab initio protein structure modeling or protein homology-related analyses.

• Journal of the Korean Institute of Rural Architecture
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• v.12 no.1
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• pp.49-56
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• 2010

This paper is aimed to clarify field technique of non-educated constructors in timber structure of korean-style secondary station(Gong-So) on the annex of catholic church. The main object of this study is Sin-Sung and Su-Bun secondary stations which post-lintel structure was 2high columns(Go-Ju) 5beams(Ryang) in Jeon-Buk area of Korea. We reached the following conclusion. Firstly, these secondary stations are required a lots of space for number of persons with the introduction of basilica plane. These plans have different intervals in the layout. Secondly, they constructed the holy space by using high columns(Nae-Jin-Go-Ju). The former problems of plan layout are sloved by reinforcement and replacement eaves and rafter of logitudinal. Thirdly, the elements showed the natural feature such as irregular wooden floor, arch-type head pentrating tie and a ceiling. In the end, we knew that non-educated constructor had found a way in the problem for accepting unreasonable work.

• Journal of the Earthquake Engineering Society of Korea
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• v.24 no.2
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• pp.87-93
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• 2020

Seismic responses due to the dynamic coupling between a primary structure and secondary system connected to a structure are analyzed in this study. The seismic responses are compared based on dynamic coupling criteria and according to the error level in the natural frequency, with the recent criteria being reliant on the error level in the spectral displacement response. The acceleration responses and relative displacement responses of a primary structure and a secondary system for a coupled model and two different decoupled models of two degrees-of-freedom system are calculated by means of the time integration method. Errors in seismic responses of the uncoupled models are reduced with the recent criteria. As the natural frequency of the secondary system increases, error in the natural frequency decreases, but seismic responses of uncoupled models can be underestimated compared to that of coupled model. Results in this paper can help determine dynamic coupling and predict uncoupled models' response conservatism.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.5
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• pp.728-733
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• 2018

Deep learning has been actively studied for predicting protein secondary structure based only on the sequence information of the amino acids constituting the protein. In this paper, we compared the performances of the convolutional neural networks of various structures to predict the protein secondary structure. To investigate the optimal depth of the layer of neural network for the prediction of protein secondary structure, the performance according to the number of layers was investigated. We also applied the structure of GoogLeNet and ResNet which constitute building blocks of many image classification methods. These methods extract various features from input data, and smooth the gradient transmission in the learning process even using the deep layer. These architectures of convolutional neural networks were modified to suit the characteristics of protein data to improve performance.

• Proceedings of the Korea Information Processing Society Conference
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• 2005.11a
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• pp.15-18
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• 2005

A ribonucleic acid (RNA) is one of the two types of nucleic acids found in living organisms. An RNA molecule represents a long chain of monomers called nucleotides. The sequence of nucleotides of an RNA molecule constitutes its primary structure, and the pattern of pairing between nucleotides determines the secondary structure of an RNA. Non-coding RNA genes produce transcripts that exert their function without ever producing proteins. Predicting the secondary structure of non-coding RNAs is very important for understanding their functions. We focus on Nussinov's algorithm as useful techniques for predicting RNA secondary structures. We introduce a new traceback matrix and scoring table to improve above algorithm. And the improved prediction algorithm provides better levels of performance than the originals.

• Journal of Microbiology
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• v.45 no.1
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• pp.79-82
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• 2007

The 58 rRNA gene from Sphingobium chungbukense DJ77 was identified. The secondary structure of the 199-base-long RNA was proposed. The two-base-long D loop was the shortest among all of the known 5S rRNAs. The U19-U64 non-canonical pair in the helix II region was uniquely found in strain DJ77 among all of the sphingomonads.

• International Journal of Contents
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• v.3 no.1
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• pp.19-23
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• 2007

Recently, several main-memory index structures have been proposed to reduce the impact of secondary cache misses. In mainmemory storage systems, secondary cache misses have a substantial effect on the performance of index structures. However, recent studies still stiffer from secondary cache misses when visiting each level of index tree. In this paper, we propose a new index structure that minimizes the total amount of cache miss latency. The proposed index structure prefetched grandchildren of a current node. The basic structure of the proposed index structure is based on that of the CSB+-Tree, which uses the concept of a node group to increase fan-out. However, the insert algorithm of the proposed index structure significantly reduces the cost of a split. The superiority of our algorithm is shown through performance evaluation.

• Journal of the Korean Magnetic Resonance Society
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• v.12 no.2
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• pp.65-73
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• 2008

HP1298 (Swiss-Prot ID ; P65108) is an 72-residue protein from Helicobacter pylori strain 26695. The function of HP1298 was identified as Translation initiation factor IF-l based on sequence homology, and HP1298 is included in IF-l family. Here, we report the sequence-specific backbone resonance assignments of HP1298. About 97% of all the $^{1}HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$, and $^{13}CO$ resonances could be assigned unambiguously. We could predict the secondary structure of HP1298, by analyzing the deviation of the $^{13}C{\alpha}$ and $^{13}C{\beta}$ shemical shifts from their respective random coil values. Secondary structure prediction shows that HP1298 consists of six $\beta$-strands. This study is a prerequisite for determining the solution structure of HP1298 and investigating the structure-function relationship of HP1298. Assigned chemical shift can be used for the study on interaction between HP1298 and other Helicobacter pylori proteins.