• Bulletin of the Korean Chemical Society
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• v.34 no.3
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• pp.815-819
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• 2013

Micropatterns of streptavidin and human epidermal carcinoma A431 cells were successfully imaged, as received and without any labeling, using cluster $Au_3{^+}$ ion beam-based time-of-flight secondary ion mass spectrometry (TOF-SIMS) together with a principal component analysis (PCA). Three different analysis ion beams ($Ga^+$, $Au^+$ and $Au_3{^+}$) were compared to obtain label-free TOF-SIMS chemical images of micropatterns of streptavidin, which were subsequently used for generating cell patterns. The image of the total positive ions obtained by the $Au_3{^+}$ primary ion beam corresponded to the actual image of micropatterns of streptavidin, whereas the total positive-ion images by $Ga^+$ or $Au^+$ primary ion beams did not. A PCA of the TOF-SIMS spectra was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from the raw data and used in the second PCA run, which resulted in a contrasted - and corrected - image of the micropatterns of streptavidin by the $Ga^+$ and $Au^+$ ion beams. The findings herein suggest that using cluster-ion analysis beams and multivariate data analysis for TOF-SIMS chemical imaging would be an effectual method for producing label-free chemical images of micropatterns of biomolecules, including proteins and cells.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1231-1240
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• 2021

Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

• Journal of the Korean Society of School Health
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• v.7 no.1
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• pp.9-28
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• 1994

This study is to investigate the status and problems in the anual physical examination in elementary & secondary schools. The study helps the introduction of health assessment. Two hundreds and forty nurse teachers of elementary & seconcary school in Seoul received in-service traomong fpr 20 daus frp, September 7 to September 27, 1992. Questionnaires were given to the 240 teachers and the number of collected questionnaire is 112. The result of the questionnaire analysis is like the following. First, the answered teachers aged 35~39, 36.6%; experience 6~10 years 37.5%; the number of class 31~40, 28.6%. Among the answered of the school doctors is practicioners. The doctors major subjects are 26.8% of internal medicine and 35.7% of surgery. Second, in elementary & secondary school nurse teachers received in-service training for the primary health assessment. And then 37.5% of schools practiced the health assessment. The term of the health assessment is largely a-year-length, occurately its rate of schools have practiced the assessment reaches 81.0%. The number of health assessment are consist of eyes, nose, ear, throat, skin, spine, heart and other abnormal regions and diseases all over the body. And 83.3% of the rate of the health assessment includes all these contents. The period of the health assessment is 7 to 28 days. Third, the physical examination conducted by school doctors is 91.0%. The method is various; one is 56.6% of the students who checked first by he nurse teacher. The number of 15.1% of the schools is directly checked by the school doctor. The invited medical doctors are divided into two categories. The number of general physician is 61.9%. Contrary to that school doctors are 38.1%. The contents of the medical examination is all the items printed on the medical examination sheet. To follow the medical examination sheet the number of school is 59.6%. Eyes, throat, skin and heart etc. partly medical examination is 40.4%. The rate of schools that used only stethoscope, tongue pressor as the medical instruments reaches 53.4% and 87.1% of elementary & secondary school give the invited doctors the allowance a nurse teachers conference decided. The number of 8.9% schools pay the doctors independent allowance. The medical examomatopm allowance is 200 to 250 won per capita. The rate is 56.1%. Forth, after the medical examination 72.1% of school sends letter to the parents to notice the result of the examination. The number of response from parent is 12.5%. The observation record in secondary school is 70.6% for junior, and 80.0% for senior respectively, and 65.5% is for the elementary school children. To conclude the regular physical examination in elementary & secondary school is very important. Because the students are in the stage of rapid growth and development. Early finding of the students diseases can help to cure with ease. In the light of public health the management of health program in the elementary & secondary school is of consequence.

• Korean Journal of Comparative Education
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• v.28 no.1
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• pp.1-24
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• 2018

The objective of the article is to find the research trends and the main traits presented in the keywords on abstracts of research articles of "International Journal of Education Development" from 2002 to 2017. To do this, Text Network Analysis(TNA) was applied targeting 966 papers on the journal and the major research outcomes are as follows. First, the frequency analysis on the keywords showed that the keywords like Administration of education program, Schools and instruction, Regional public administration, Educational support service, Elementary education, and Elementary and secondary school were analyzed more than 100 times and also high in centrality degree. Second, the analysis results of the keywords presented in those research articles by development goal periods showed that several new keywords like Elementary education, Elementary and secondary school, Education quality, Secondary education, Educational planning have emerged frequently after SDGs and these keywords showed high in their centrality analysis. Third, the analysis on education level showed that the keywords like Elementary education, Administration of education program, School children were high in frequency and centrality degree in Elementary level. In secondary level, Schools and instruction, Administration of education program, Academic achievement were high, and in high level, college and university was high, respectively.

• Journal of the Korean Magnetic Resonance Society
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• v.3 no.2
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• pp.90-99
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• 1999

Doping of boron atoms in graphite has been well known method to increase the discharge capacity as the negative electrode material for lithium secondary battery. Herein, the boron-doped graphites are prepared by mixing 1, 2.5, 5, and 7 wt. % of boron carbide in carbon during the graphitizing process. The structural states of boron in boron-doped graphites are investigated by solid-state 11B NMR spectroscopy. The resonance lines for substitutional boron atoms are identified as the second order quadrupolar powder pattern with the quardrupole coupling constant, QCC = 3.36(2) MHz. The quantitative analysis of 11B NMR spectra with boron-doped graphite has also been performed via simulation.

• 유체기계공업학회:학술대회논문집
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• 2000.12a
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• pp.312-317
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• 2000

High efficient steam turbine stage has been developed with the help of the 3-dimensional design tool. In this stage design, the compound leaned stacking method has been adopted to reduce the secondary flow loss of a turbine passage and to increase the performance efficiency for the turbine nozzles. And the turbine buckets have been designed with the quasi-3-dimensional turbomachinery blade design method. To verify the stage design, therefore, the 3-dimensional numerical simulation of a steam turbine stage was conducted. In this design, CFX-TASCflow was employed to predict the turbulent flow of a steam turbine stage. The analysis was performed in parallel calculation using the HP N4000 8 CPUs machine. The result showed CFX-TASCflow could be used as the 3-dimensional flow analysis tool of steam turbine design.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.12 no.7
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• pp.674-679
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• 2000

The Sirocco fan performance and its three-dimensional flow characteristics were numerically predicted by STAR-CD software. Turbulent flow computations were performed using approximately 500,000 mesh points, and the performance results of two computational methods, transient analysis and quasi-static analysis were compared with experimental data. At present, our attention was focused on localizing the three-dimensional flow characteristics of the Sirocco fin, especially the structure of the secondary flow in the scroll and the through-flow characteristics of the Sirocco fan blades. Also, for an optimization, the scroll passage was tilted with 10 degrees to change the flow characteristics and improve the performance of the Sirocco fan.

• Proceedings of the Korean Nuclear Society Conference
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• 1998.05b
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• pp.837-843
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• 1998

This paper presents the thermomechanical and fracture mechanics evaluation procedure of thermal striping damage on the secondary piping of LMFR using Green's function method and standard FEM. The thermohydraulic loading conditions used in the present analysis are simplified sinusoidal thermal loads and the random type data thermal load. The thermomechainical fatigue damage was evaluated according to ASME code subsectionNH. The analysis results of fatigue for the sinusoidal and random load cases show that fatigue failure would occur at a geometrically discontinuous location during 90,000 hours of operation The fracture mechanics analysis showed that the crack would be initiated at an early stage of the operation. The fatigue crack was evaluated to propagate up to 5 ㎜ along the thickness direction during the first 944 and 1083 hours of operation for the sinusoidal and the random loading cases, respectively.

• Proceedings of the KSME Conference
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• 2003.11a
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• pp.396-401
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• 2003

In the present study, preliminary investigation were carried out by analysis of energy system(heat and electricity) based on phosphoric acid fuel-cell of 50 kW for eco-apartment houses. Analysis model were consisted of fuel cell energy system, secondary energy unit and residential building of 5 stories with 20 and 40 households. And the investigation results reviewed under load pattern of heat and electric power of the apartment houses. The results showed mismatch between the needed heat load pattern and output of fuel cell energy system. The mismatch rate were assessed about 10-180% of heat load for apartment houses with season. We found that secondary energy unit are needed in order to supply insufficient heat.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.26 no.4
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• pp.469-474
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• 2016

Abnormal sound was heard from a heat exchanger of condensate water system in a nuclear power plant, which was identified as impact sound of a loose part later. Nuclear power plants are normally equipped with loose part monitoring system for primary water system, but not for secondary water system. The abnormal sound was analyzed by using the impact signal-processing methodology based on the Hertz theory. The predicted results for impact location and size of the loose part showed good agreement with those of the actual loose part found during the overhaul period in the plant. So, this analysis methodology for the impact signal will be widely utilized for the primary and secondary side of the nuclear power plant.