• Journal of Plant Biotechnology
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• 제43권3호
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• pp.332-340
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• 2016

Seed size traits are controlled by multiple genes in crops and determine grain yield, quality and appearance. However, the molecular mechanisms controlling the size of plant seeds remain unclear. We performed functional analysis of BrPATL4 encoding Sec14-like protein to determine the genetic architecture of seed size, shape and their association analyses. We used 60 $T_3$ transgenic rice lines to evaluate seed length, seed width and seed height as seed size traits, and the ratios of these values as seed shape traits. Pleiotropic effects on general architecture included small seed size, erect panicles, decreased grain weight, reduced plant height and increased sterility, which are common to other mutants deficient in gibberellic acid (GA) biosynthesis. To test whether BrPATL4 overexpression is deleterious for GA signal transduction, we compared the relative expression of GA related gene and the growth rate of second leaf sheath supplied with exogenous $GA_3$. Overexpression of BrPATL4 did not affect GA biosynthesis or signaling pathway, with the same response shown under GA treatment compared to the wild type. However, the causal genes for the small seed phenotype (D1, SRS1, and SRS5) and the erection of panicles showed significantly decreased levels in mRNA accumulation compared to the wild type. These results suggest that the overexpression of BrPATL4 can control seed size through the suppression of those genes related to seed size regulation. Although the molecular function of BrPATL4 is not clear for small seed and erect panicles of BrPALT4 overexpression line, this study provides some clues about the genetic engineering of rice seed architecture.

• 생명과학회지
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• 제30권10호
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• pp.844-850
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• 2020

본 연구는 지구온난화와 태풍에 의해 수확기의 손실을 막기 위해 벼의 출수기를 당기는 유전자를 찾는 것을 목표로 한다. 청청/낙동 배가반수체(CNDH)와 모본인 청청, 부본인 낙동을 재료로 사용하여 QTL을 이용해 출수기 관련 유전자의 위치를 조사하고 gene을 cloning하여 염기서열을 분석하였다. 분석결과 염색체 8번에 13개의 contig가 있었고 그 중 출수기와 관련된 1개의 ORF가 존재했다. 단백질 서열을 분석한 결과 벼의 Os08g0341700, 그리고 AtSFH13, AtSFH7 단백질과 유사한 것으로 보인다. 신호전달과 관계가 있는 Os08g0341700은 phosphatidylinositol transfer-like protein II와 유사하며 아직 완전한 information은 밝혀지지 않았다. 하지만 Sec14P와 연관이 있으며 세포 생장 등에 관여하는 phosphatidylinositol 특이적 신호전달 경로의 역할을 할 것으로 추정 중이다.