• Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.129-136
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• 2014

Using the criteria of previous study, we analyzed the morphological characters of 66 specimens of the Sebastes pachycephalus complex collected from Korea between 2008 and 2013. As a result, the 37 specimens were identified as Sebastes nudus, and the remaining specimens as Sebastes pachycephalus. Sebastes nudus is characterized by the absence of scales below the base of the dorsal fin spines; the absence of dark spots on the dorsal, anal, and caudal fins; and the presence of distinct yellow or brownish-red markings on the dorsum (when fresh). The most similar species S. pachycephalus differs from S. nudus in having minute scales below the base of the dorsal fin spines. We propose the new Korean name "Hwang-jeom-gae-bol-rak" for S. nudus, and redescribe S. pachycephalus.

• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.1-9
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• 1996

Egg and larval stages of the rockfish, Sebastes thompsoni and S. pachycephalus pachycephalus, are described and illustrared. The size of these eggs when released were 1.27~1.33mm in S. thompsoni and 1.39~1.52mm in S. pachycephalus pachycephalus. The size of these hatching larvae when released were 3.02~3.12mm TL (total length) in S. thompsoni and 4.83~5.17mm in S. pachycephalus pachycephalus. Notochord flexion occured 7.56~8.00mm TL (17~20 days after hatching) in S. thompsorzi and 5.43~5.56mm in S. pachycephalus pachycephalus. Preflexion and flexion larvae (averaged 3.97~7.78mm TL) of S. thompsoni were distinguished from related species by pigmentation of the dorsal and ventral midlines of the tail. Larvae of S. pachycephalus pachycephalus were distinguished from related species by pigmentation of the dorsal and ventral midlines of the trunk. Transformation occured 14.22~15.86mm TL in S. pachycephalus pachycephalus.

• Korean Journal of Ichthyology
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• v.32 no.3
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• pp.117-129
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• 2020

The body color pattern in fish is a distinctive feature for species identification. The blass bloched rockfish Sebastes pachycephalus is a commercially important marine fish species, distributed in the central and southern parts of Korea and south Hokkaido of Japan. It has a morphological feature divided into four subspecies according to with or lacking distinct spots on the body surface, and to the location of markings on the body surface. However, the genetic basis of body color pattern of S. pachycephalus is still unknown. Thus we analyzed the transcriptome of S. pachycephalus skin samples using RNA-seq analysis to investigate functional genes related to body color patterns. The experimental skin samples were prepared by classified into 'Wild type' (lacking distinct spots and markings) and 'Color type' (with distinct spots and marking). Two skin sample transcriptomes were compared pairwise and the results revealed that were 164 differentially expressed unigenes in the skin samples of 'Wild type' and 'Color type'. Gene Ontology analysis of 164 differentially expressed unigenes showed that these genes were included in the functional group of molecular function (2 genes), biological process (46 genes), and cellular component (6 genes). There were several genes that body color type skin specific expression and the genes were CTL (Galactose-specific lectin nattectin), CUL1 (Cullin-1), CMAS (N-acylneuraminate cytidylyltransferase), NMRK2 (Nicotinamide riboside kinase 2), ALOXE3 (Hydroperoxide isomerase ALOXE3), SLC4A7 (sodium bicarbonate cotransporter 3). Our study is the first attempt to search for functional genes involved in the formation of body color patterns in S. pachycephalus. The differentially expressed unigenes obtained in this study can be used as candidate genes for functional gene study related to body coloration of fish.

• Journal of Aquaculture
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• v.21 no.1
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• pp.7-12
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• 2008

Toxicity of ten commercial disinfectants(hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds(QAC), didecyl dimethyl ammonium chloride(DDAC), ortho-dichlorobenzene, and copper sulfate) was measured by chinook salmon embryo-214 cell line and three fish species: flounder Paralichthys olivaceus, black rockfish Sebastes pachycephalus, and black sea bream Acanthopagrus schlegelii. The $LC_{50}$ levels of 24 hours acute toxicity with the ten disinfectants were tested in three species of fish. Effectiveness of ten chemical disinfectants were varied by each chemical as well as by species. Hydrogen peroxide showed the higest activity at 201, 269, and 139 ppm in the flounder, the black rockfish, and black sea bream, respectively. DDAC showed the lowest activity at 2.1, 1.0, and 1.5 ppm in the flounder, the black rockfish, and black sea bream, respectively. The highest variation was observed in copper sulfate by both the chemicals and the species.

• Korean Journal of Ichthyology
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• v.33 no.4
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• pp.226-239
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• 2021

Sebastes minor, Sebastes trivittatus, Sebastes owstoni, and Sebastes steindachneri are indigenous fish species inhabiting the central part of the East Sea, Korea. In order to understand the molecular evolution of these four rockfishes, we sequenced the complete mitochondrial genomes (mitogenomes) of S. minor and S. trivittatus. To further analyze the phylogeny of Sebastes species, the mitogenomes of 16 rockfishes were comparatively investigated. The complete mitochondrial DNA (mtDNA) nucleotide sequences of S. minor and S. trivittatus were 16,408 bp and 16,409 bp in length, respectively. A total of 37 genes were found in mtDNA of S. minor and S. trivittatus, including 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which exhibited similar characters with other Sebastes species in the East Sea, Korea. In addition, we detected a conserved motif "ATGTA" in the control region of the four Sebastes species, but no tandem repeat units. Comparative analyses of the congeneric mitochondrial genomes were performed, which showed that control regions were more variable than the concatenated protein-coding genes. As a result of analysing phylogenetic relationships of four Sebastes species by using concatenated nucleotide sequences of 13 protein-coding genes, S. minor, S. trivittatus, S. owstoni and S. steindachneri were clustered into three clades. The phylogenetic tree exhibited that S. minor and S. steindachneri shared a closer relationship, whereas S. trivittatus and S. vulpes formed another distinct clade. Our results contribute to a better understanding of evolutionary patterns of Sebastes species inhabiting the middle East Sea, Korea.

• Korean Journal of Ichthyology
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• v.3 no.1
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• pp.11-23
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• 1991

To study the distribution of inhthyoplankton in the adjacent water of Wolsong, samples were collected using Bongo net from the surface water in November of 1989, and February, May and July of 1990. Fish eggs were divided into Engrualis japonicus, Callionymidae, Maurolicus muelleri, Konosirus punctatus, and others. The dominant species of fish eggs were Maurolicus muelleri and Callionymidae accounting for 60.1% and 13.7% of the total egg abundance, respectively. Total of 21 taxa of fish larvae occurred. The predominant species was Engraulis japonicas accounting for 61.2% of the total lavae, and bollowed by Callionymidae(11.9%). Other major species were Maurolicus muelleri, Gobiidae sp., Ammodytes personatus, Sebastes inermis, S. Pachycephalus, Scartella cristata, and Sebastiscus marmoratus. These 9 taxa constituted 96.5% of the total larvae collected. In this area, where upwelling occasionally occurs the distribution of fish eggs and larvae appeared to be affected by the transport of the surface water during the upwelling period.

• Food Science and Biotechnology
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• v.17 no.5
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• pp.971-975
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• 2008

This study was to examine the potential disinfection efficiencies of 10 compounds by determining their antimicrobial capacity and ichthyotoxicity. Antimicrobial effects against Vibrio sp., Edwadsiella tarda, Streptococcus sp., and Staphylococcus sp. were tested using 10 different disinfectants; hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds (QACs), didecyl dimethyl ammonium chloride (DDAC), ortho-dichlorobenzen, and copper sulfate. Chlorine dioxide ($ClO_2$) containing 5% $ClO_2$ and copper sulfate had no effects on bactericidal activity, while the other disinfectants resulted in 99.99% bactericidal activity against 4 strains of fish pathogenic bacteria. The ichthyotoxicity of the 10 disinfectants was investigated using 3 kinds of fish species; flounder (Paralichthys olivaceus), rockfish (Sebastes pachycephalus), and black sea bream (Acanthopagrus schlegelii). Median lethal concentration ($LC_{50}$) values of the 10 disinfectants were estimated to determine toxicity ranges of the doses within 24 hr. Among test disinfectant solutions, hydrogen peroxide showed the highest $LC_50$ in flounder (201.3), rockfish (269.7), and black sea bream (139.3 ppm). DDAC revealed the lowest $LC_{50}$ in flounder (2.1), rockfish (1.0), and black sea bream (1.5 ppm). These results suggest that DDAC, quaternary ammonium compounds, glutaraldehyde, and sodium hypochlorite are effective disinfectants for fish and bacterial species examined in this study.

• Journal of Marine Life Science
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• v.3 no.1
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• pp.22-30
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• 2018

In this study, pilot study on the introduction of stationary fishery was performed to solve the problem of fisheries resource reduction. The Fyke net, which is a test fishing net was selected considering the environment, operation and management costs of Ulleungdo, conditions that can be operated by small fishing vessels and personnel. As a result of 11 times survey using Fyke net from April to May 2017, 2,735 individuals and 983.4 kg caught and the dominant species were red seabream, yellowtail, olive flounder, mitra squid, horse mackerel, filefish, etc. In conclusion, if the production of squid, which is one of the major fisheries resources of Ulleungdo, is continuously decreased, it is considered that introduction of small-scale stationary fishery such as Fyke net would be useful as a means replace income of fishermen.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.550-563
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• 2000

This study was carried out in order to elucidate the fundamental data on the taste compounds between wild and cultured fishes produced on Chungmu and Wando at the southern coast areas of Korea. For this purpose, the food components of cultured fishes such as red sea bream Pagnus major, Sebastes pachycephalus and flounder, Paralichthys olivaceus being spot lighted for the main sea fish, the staple food and high economic fish were investigated and compared with those of the wild ones. There was a little appreciable difference in the proximate compositions of all the species from localities between wild and cultured fishes. But according to the growing conditions, wild fishes were higher in moisture contents and lower in crude lipid content than those of cultured fishes and little difference was seen in protein and ash contents between the two. With regard to the nucleotides and their related compounds, i.e. ADP, IMP and inosine were detected but ATP and hypoxanathine were not from them. On the other hand, there were little difference in the total taste compounds of all the species from localities and the growing conditions between wild and cultured fishes. But all the species were higher in IMP content. The total of seventeen amino acids were detected in samples. The highly contents of glutamic acid, lysine, aspartic acid, proline, leucine, alanine, valine and alginine were showed and less low contents of cystein, histidine, methionine, tyrosine and phenylalanine were detected. The total amino acids of the others were much alike in that composition. Little difference was seen from localities and the growing conditions between wild and cultured ones. The free amino acids were much alike in that composition of all the species. There was little difference in the free amino acid compositions all the species from localities and the growing conditions between wild and cultured fishes. But taurine was dominant, showing from 39% to 65% of the free amino acid content and it is followed by hydroxyproline, lysine, alanine and glycine in other. There were differences in the organic acid compositions of all the species from localities and the growing conditions between wild and cultured fishes. In addition, cultured fishes were more abundant in the total organic acid compositions than those of wild ones.