• 미생물학회지
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• 제49권3호
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• pp.237-244
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• 2013

16S rRNA 유전자를 대상으로 454 파이로서열 분석법을 이용하여 해삼(Apostichopus japonicus)과 새우(Litopenaeus vannamei)의 장내 세균의 다양성을 분석하였다. 해삼의 경우, 대부분의 서열은 두 개의 속과 연관성이 있는 것으로 나타났다. 하나는 Fusobacteria 문의 Propionigenium 속이며, 다른 하나는 Bacteroidetes 문의 Flavobacteriaceae 과에 속하는 미분류 속이었다. 새우는 해삼에 비해 다양한 속들을 포함하고 있었으며 Lactococcus, Leuconostoc, Prochlorococcus, Vibrio 속들과 Flavobacteriaceae, Rhodobacteraceae, Desulfobulbaceae, Helicobacteraceae 과와 Mycoplasmatales 목에 속하는 미분류 속들을 포함하고 있었다. 해삼과 새우의 속들의 절반 이상이 환경에서 비배양적으로 얻어진 서열만 존재하는 미분류된 속인 것으로 확인되었다. 대용량 454 파이로서열 분석법을 통하여 해삼과 새우의 장내 세균의 다양성을 밝힐 수 있었으며, 그 결과 해삼과 새우는 아직까지 밝혀지지 않는 새로운 미생물을 많이 포함하고 있는 것을 알 수 있었다.

• 한국식품영양과학회지
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• 제12권4호
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• pp.342-349
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• 1983

수산명산품(水産名産品)에 속(屬)하는 해삼내장(內臟)젓갈이 식품영양학적(食品營養學的)인 평가(評價) 및 그 가공(加工)에 관(關)한 기초자료(基礎資料)를 얻고자 본 연구를 착수하였으며, 해삼내장(內臟) 조직중(組織中)에 분포(分布)하는 단백질분해(蛋白質分解) 조효소(粗酵素)를 추출(抽出)하여 활성조건(活性條件)과 젓갈 숙성중(熟成中)의 유리(遊離)아미노산(酸) 및 단백질(蛋白質) 구성(構成) 아미노산(酸)의 조성변화(組成變化)에 관(關)하여 분석(分析) 검토(檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 해삼내장(內臟) 조직중(組織中)에는 pH 3.1 $50^{\circ}C$, pH 5.7 $50^{\circ}C$ 그리고 pH 7.7 $45^{\circ}C$에 각각(各各) 최적활성조건(最適活性條件)을 갖는 세 종류(種類) 이상(以上)의 단백질분해효소(蛋白質分解酵素)의 존재(存在)가 확인(確認)되었다. 2. 이들 효소중(酵素中) pH 3.1 $50^{\circ}C$, pH 7.7 $45^{\circ}C$에 최적활성(最適活性)을 갖는 효소(酵素)들은 식염농도(食鹽濃度) 1%이상(以上)의 농도(濃度)에서 점차(漸次) 높은 조해(阻害)를 받았으며, pH 5.7 $50^{\circ}C$에 최적활성조건(最適活性條件)을 갖는 효소(酵素)는 식염농도(食鹽濃度) 1%에서 부활(賦活) 되었으며, 5%이상(以上)에서 조해(阻害)를 받았다. 3. 몇가지 염류(鹽類)이온에 의한 영향(影響)을 검토(檢討)한 결과(結果) pH 3.1 $50^{\circ}C$에서 최적조건(最適條件)을 보인 효소(酵素)는 실험(實驗)에 쓴 전(全) 염류(鹽類)이온에 의하여 조금씩 조해(阻害)를 받았으며, pH 5.7 $50^{\circ}C$에서 최적조건(最適條件)을 보인 효소(酵素)는 $Cu^{2+}$을 제외(除外)한 모든 염류(鹽類)이온에 의하여 부활(賦活)을 받았고, pH 7.7 $45^{\circ}C$에서 최적조건(最適條件)을 보인 효소(酵素)는 $Ca^{2+}$과 $Mn^{2+}$에 의하여서는 부활(賦活)을 받았으며, $Ba^{2+}$에 의하여서는 영향(影響)이 없었고 $Co^{2+}$, $Mg^{2+}$에 의하여서는 조해(阻害)를 받았다. 그리고 특(特)히 이 효소(酵素)들은 $Cu^{2+}$에 의하여서는 심(甚)한 조해(阻害)를 받았다. 4. 해삼내장(內臟) 조직(組織)에 분포(分布)하는 효소(酵素)들의 젓갈숙성일수(熟成日數)의 경과(經過)에 따른 활성(活性)에 미치는 영향(影響)을 검토(檢討)한 결과(結果), pH 3.1 $50^{\circ}C$와 pH 7.7 $45^{\circ}C$에서 활성최적조건(活性最適條件)을 보인 효소(酵素)들은 활성(活性)이 조금씩 약화(弱化)하여 갔으나, pH 5.7에서 활성최적조건(活性最適條件)을 보인 효소(酵素)는 숙성일수(熟成日數)의 경과(經過)에도 영향(影響)이 없었다. 5. 해삼내장(內臟)젓갈 숙성(熟成) 8일(日)째일 때는 생(生) 내장중(內臟中)의 단백질(蛋白質) 구성(構成)아미노산(酸)의 대부분(大部分)이 감소(減少)하였으며, 특(特)히 두드러지게 감소(減少)한 아미노산(酸)은 arginine, alanine, glutamic acid, glycine, serine, valine, threonine 및 lysine 등(等)이었고, methionine와 histidine 및 isoleucine 등(等)은 그 감소폭(減少幅)이 낮았다. 6. 유리(遊離)아미노산(酸) 조성(組成)을 분석(分析)한 결과(結果), 8일간(日間) 숙성(熟成)한 시료중(試料中)에는 glutamic acid, aspartic acid, leuine 및 lysine은 많은 량(量)이 함유(含有)되어 있었고, histidine, methionine, proline 및 tyiosine 등(等)은 그 함량(含量)이 낮았다. 그리고 젓갈 숙성과정중(熟成過程中)에는 대부분(大部分)의 유리(遊離)아미노산(酸)은 증가(增加)하였으며, 특(特)히 lysine, histidine, threonine, glutamic acid, methionine, valine, leucine 등(等)은 많은 증가(增加)를 보였다.

• 한국수산과학회지
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• 제50권5호
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• pp.494-499
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• 2017

This study evaluated the abundance of fecal coliforms in oysters Crassostrea gigas, sea squirts Halocynthia roretzi and sea cucumbers Apostichopus japonicus in fisheries along the coast of Korea in 2014, and investigated the prevalence of antimicrobial resistance in Escherichia coli isolated from these fishery products. The ranges of fecal coliforms found in oysters, sea squirts and sea cucumbers were <18-20, <18-330 and <18-3,300 MPN (most probable number)/100 g, respectively. Sea squirts contained the greatest range of E. coli (<20-140 MPN/100 g), followed by sea cucumbers (<20-130 MPN/100 g) and oysters (<20-20 MPN/100 g). A total of 26 strains of E. coli were isolated from 34 sea squirt, 25 sea cucumber and 13 oyster samples. Strains thus isolated were tested for their susceptibility to 22 antimicrobial agents used in Korea for medical or veterinary therapy. E. coli isolates showed the greatest resistance to ampicillin (84.6%), followed by trimethoprim (34.6%), nalidixic acid (34.6%), tetracycline (30.8%), pipemidic acid (26.9%), streptomycin (23.1%), chloramphenicol (23.1%), trimethoprim/sulfamethoxazole (23.1%), and gentamicin (15.4%). Resistance to at least one antimicrobial agent was present in 88.5% of E. coli isolates. Of the 26 isolated, six strains (23.1%) were resistant to multiple antimicrobial agents.

• 수산해양기술연구
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• 제50권4호
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• pp.542-550
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• 2014

We investigated biomass, diatom species and fucoxanthin contents as cell growth, fatty acid and amino acid contents as nutritional composition of diatoms attached on plate to confirm effects of light emitting diodes (LEDs) due to block off natural light. In the single LED irradiation, biomass showed significantly higher to $30.0{\pm}6.48mg/m^2$ in white LED than that of others (P<0.05). The dominate diatom species was Navicula cancellata. Their lipid contents showed significantly higher to $112.9{\pm}19.23ug/mg$ dry matter (DM) in control than that of others LEDs. But eicosapetaenoic acid (EPA) contents showed significantly higher to $3.3{\pm}0.62ug/mg$ DM than others, but not significantly differed with natural control light treatment (P<0.05). And total protein contents are higher in control and blue LED light than that of others, but essential amino acid contents showed significantly higher to $3.2{\pm}4.8%$ in control (P<0.05). In mixing light with natural and LED light, biomass showed $2.6{\pm}0.22mg/m^2$ in blue LED (P<0.05). Fatty acids contents were not significantly differed with all treatments. Amino acid contents showed to $11.0{\pm}0.33ug/mg$ DM in white LED (P<0.05), but not significantly differed with others LED lights (P>0.05). Therefore, we could suggest that irradiation of blue LED in natural light very benefit to diatom culture for larvae of sea cucumber and abalone and do on.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 제45회 학술심포지움 및 추계학술대회
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• pp.57-57
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• 2005

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 제45회 학술심포지움 및 추계학술대회
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• pp.56-56
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• 2005

• 동력기계공학회지
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• 제15권3호
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• pp.46-51
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• 2011

Low temperature vacuum drying technique, whose drying time and quantity of exhausting energy is about 25~30% of hot air drying, is very excellent in the drying efficiency. This paper is made out in the aspects of heat engineering with the object of developing Korean drying machine which can dry once a large quantity of objects to be dried in the state of low temperature and vacuum. As the results, it took about 17 hours(3~4 days in case of hot air drying) for material to reach about 18% of the final moisture content in order to store products for a long time, from about 78~80% of the early moisture content at the beginning of drying, and maximum drying rate comes to about 0.35 kg/m2hr at about 400% of the moisture content.

• 한국자원식물학회지
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• 제10권3호
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• pp.271-273
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• 1997

This study was carried out to obtain basic envirinmental information of the native area and growth characteristics at different age of Dendropanax morbifera LEV. In the southern Korea. The Dendropanax morbifera LEV. Was grown naturally at Wando, Cheju(Mt. Halla), Yecheon(Kermoon island),Sinan(Hong isand), Jindo(Mt. Chermchill) and Haenam(Mt. Taeroon), all between 30 to 450m above the sea level. The soil characterisitics of the native area had a pH of 5.3 to 5.4 with 8.4 to 9.8% content of organic matter and 18.2 to 21.3% soil moisture. Flowering starting in the middle of July begun when the trees were 6 years old. Six year old trees are 129 cm in stem height, 34 mm in stem diameter,15.4 in number of leaves with petioles 12.9 cm long. Xylem sap can be obtained from over 10 year old trees that grew well and have over 10 cm stems in diameter.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.177-177
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• 2005

• 동아시아식생활학회지
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• 제1권3호
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• pp.259-276
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• 1991

The review of side dishes, oereal dishes and seasonings of the banquet on the 24th year of King Kojong in Chosun Dynasty were summarized as follows: 1. Ten kinds of soups(=Tang), that is Yyolguja Tang$.$Guja Tang$.$Keumjung Tang$.$Whanja Tang$.$Jap Tang$.$Gol Tang$.$Seunggiwa Tang$.$Jeopo Tang$.$Haesam Tang$.$Yang Tang were served. The most frequently served soup among them was Yyolguja Tang. 2. Various kinds of dried meat/fish were piled up most highly among dishes. Sliced beef and minced beef among dried meats, white cud$.$yellow cud$.$flatfish$.$skate$.$shark$.$octopus$.$dried abalone$.$cuttle fish$.$dried scallop were piled up together. 3. Sliced of boiled beef/pork and gelatined knuckles were assorted. Boiled meat which was made of beef, pork, lamb, liver, stomach, brisket and plat flank of caw was chiefly used for boiled beef, hams for boiled pork, mediumsized lamb for boiled lamb. And the legs and stomach of caw, old chicken, mullet were used for gelatined knuckle. 4. Three kinds of pan-frying dishes that was stomach and liver of caw, and mullet were usually piled up, If one kind in one dish, sea cucumber and mullet were used respectively. 5. Boiled abalone was dried abalone with soaked, sliced and boiled down in soysauce. 6. Roasted dish was usually made of assorted food stuffs. Sometimes chicken an pheasant was used for roasted dish. 7. Steamed dish was like this : assorted beef, fresh abalone, sea cucumber, mullet, young pork, chicken, dried abalone. 8. Raw dish was made of intestine of caw, fresh abalone and mullet. 9. Poached egg was serving decorated with red pepper, pine-nut and green onion. 10. Seasoned green-been-jelly was served only one time. 11. Radish kimchi was served only one time. 12. Chief foods were nuddle and stuffed bun. 13. Served seasings were honey, mustard, soysauce with vinegar, salt. Review from banquet menu, 34 kinds of side dishes were served : 10 kinds of soup, dried fish/meat, boiled beef, boiled pork, gelatined knuckle, 5 kinds of panfrying, boiled-down-abalone in soysauce, 3 kinds of roast, 5 kinds of steamed, 3 kinds of raw dishes, pouched egg, green-bean-jelly, kimchi. 4 kinds of chief foods were served : nuddle, dry nuddle, 2 kinds of dumping.