• 한국식품조리과학회지
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• 제17권4호
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• pp.344-352
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• 2001

본 연구에서는 streptozotocin으로 당뇨를 유발시킨 흰쥐에게 methanol로 추출하여 분획한 참마의 $H_2O$ 분획물을 경구투여하고 selenium(Se)은 식이로 보충하여 당뇨시 14일간 에너지대사물 수준과 지질과산화에 미치는 영향을 실험하여 다음과 같은 결과를 얻었다. 간장과 근육의 glycogen 함량의 변화는 정상군과 당뇨대조군 사이에 유의적인 차이를 보이지 않았으며 당뇨실험군 사이에도 유의적인 차이를 보이지 않았다. 간장, 신장 및 근육의 단백질 수준도 정상군과 당뇨대조군에서 유의적이 아니었으나 근육단백질 수준이 당뇨대조군보다 DJR-Se 병용 투여군에서 높았다. 간장의 콜레스테롤 수준은 정상군과 당뇨대조군 사이에 유의적이었으며 당뇨대조군에 비해 당뇨실험군 모두에서 콜레스테롤 수준이 낮았으나 유의성은 보이지 않았다. 간장의 중성지방 수준은 당뇨대조군에 비해 DJT-Se군에서 유의적으로 높아 참마와 Se 처리의 상승작용을 보였다. 지질과산화에 영향을 주는 MDA 수준은 간장의 경우 정상군과 당뇨대조군 사이에 유의적이 아니었고 폐의 MDA 수준은 실험군과 당뇨군 모두에서 가장 낮은 수준으로 당뇨대조군에 비해 Se군에서 유의적으로 낮았다. 간장의 MDA 수준은 유의적인 차이는 아니었지만 정상군에 비해 당뇨대조군에서 높은 수준이었고 DJT-Se 병용투여군에서 모든 실험군 중 유의적으로 가장 낮은 수준이었다. 본 연구 결과 STZ 당뇨 쥐에서 참마의 $H_2O$ 분획물과 Se 투여시 지질과산화에 미치는 영향은 간장에서 MDA 수준이 가장 높았다. Se 단독 투여시 제일 큰 영향을 받는 장기는 폐였으며 다음이 신장으로 간장의 MDA 수준을 낮추는데는 DJT-Se 병용투여시 가장 효과적이었다. 참마의 $H_2O$ 분획물투여와 Se 보충은 전보의 연구 결과와 유사하게 참마나 Se를 독립적으로 섭취하는 것보다는 병용하여 보충하는 것이 지방대사물과 지질과산화에 작용하여 항산화효과에 상승작용을 보였으며 참마는 항산화에 영향하는 생리활성성분을 함유하는 식품으로 당뇨식이 처방에 유용하리라 생각된다.

• 한국식품영양학회:학술대회논문집
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• 한국식품영양학회 2003년도 하계 학술 심포지엄
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• pp.77-77
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• 2003

This study was performed to investigate the antioxidative effect as the inhibition of MDA and BSA conjugation reaction, inhibition of lipid peroxidation and the scavenging effect on DPPH radical, and antimutagenic capacities as the Ames test in 7 common vegetables taken by Korean for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food. The water extracted fractions of perilla leaves were most effective in the inhibition of MDA and BSA conjugation reaction showing 77.2% of inhibition rate among 7 vegetables. The inhibition rates of ethanol extracted fractions of sedum and wild water dropwort on the lipid peroxidation were 67.1% and 61.5%, respectively. The ethanol extracted fractions of crown daisy and wild water dropwort showed the most effective results among 7 vegetables in the DPPH radical scavenging capacities showing inhibition rate of 78.8% and 73.6%, respectively. The indirect and direct antimutagenic effects of ethanol extract of 7 vegetables were examined by Ames test using Salmonella typimurium TA98 and TA100. Inhibitory effects of wild water dropwort was superior to the other vegetables on the Ames test. These results suggest that common 7 vegetables taken by Korean are believed to be a possible antioxidative and antimutagenic capacities, although the resultswere different, some or less, according to the assay method and vegetables used.

• Environmental Analysis Health and Toxicology
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• 제19권3호
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• pp.279-286
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• 2004

Although numerous studies have shown that cadmium disturbs the normal biological processes in central nervous system, the mechanism of toxicity is not well understood. The present study has investigated the effect of cadmium on oxidative stress, Na$^{+}$K$^{+}$ ATPase activity and the aggregation of amyloid beta peptide ($\beta$-amyloid) in neuronal cell line, HT22 cell. LC$_{5}$ and LC$_{50}$ of cadmium for HT22 cell resulted from MTT assay was 4.1 uM and 9.5 uM, respectively. Cadmium (2 to 8 uM) dose-dependently increased the lipid peroxidation and decreased the content of glutathione. Cadmium 4 uM showed a significant decrease in Na$^{+}$/K $^{+}$ ATPase activity as compared with control group. The aggregation of $\beta$-amyloid was accelerated in a dose-dependent manner by the treatment with 2 to 8 uM cadmium. These results suggest that the neurotoxicity of cadmium can be mediated by the increase in oxidative stress and decrease in Na$^{+}$/K$^{+}$ ATPase activity.se activity.

• 대한한방내과학회지
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• 제20권1호
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• pp.83-98
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• 1999

This study is designed to investigate the effects of Samul-Tang extract on the response of lactic dehydrogenase(LDH) release, cellular activity, lipid peroxidation, DNA synthesis and the changes of total protein of bovine pulmonary artery endothelial cells(PAEC) from hydrogen peroxide$(H_2O_2)$-induced injury. The results are as follows : 1. Samul-Tang significantly decreased $H_2O_2$-induced release of LDH from injured bovine PAEC. 2. Samul-Tang significantly repressed $H_2O_2$-induced cellular activity from injured bovine PAEC. 3. Samul-Tang significantly repressed $H_2O_2$-induced lipid peroxidation from injured bovine PAEC. 4. Samul-Tang significantly stimulated DNA synthesis in bovine PAEC. 5. Samul-Tang significantly repressed $H_2O_2$-induced changes of total protein volume from injured bovine PAEC. Above results suggest that Samul-Tang can protect bovine PAEC from $H_2O_2$-induced injury. These results can be effectively applied to the prevention and cure of cardiovascular and cerebrovascular diseases.

• 한국자원식물학회지
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• 제22권6호
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• pp.498-505
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• 2009

The plant Agastache rugosa Kuntze has various physiological and pharmacological activities. Especially, it has been regarded as a valuable source for the treatment of anti-inflammatory and oxidative stress-induced disorders. However, little has been known about the functional role of it on oxidative damage in mammalian cells by ROS. In this study, we investigated the DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging capacity, and $Fe^{2+}$ chelating activity of the extracts from Agastache rugosa. In addition, we evaluated whether the extract can be capable of reducing $H_2O_2$-induced DNA and cell damage in NIH 3T3 cells. These extracts showed a dose-dependent free radical scavenging capacity and a protective effect on DNA damage and the lipid peroxidation causing the cell damage by $H_2O_2$. Therefore, these results suggest that Agastache rugosa is useful as a herbal medicine for the chemoprevention against oxidative carcinogenesis.

• 한국자원식물학회지
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• 제21권6호
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• pp.449-456
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• 2008

The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and $Fe^{2+}$ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death.

• 한국식품영양과학회지
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• 제38권1호
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• pp.19-24
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• 2009

식용 및 약용으로 예로부터 널리 이용하는 양송이버섯 추출물의 생리적 기능에 대한 활성을 탐색하기 위하여 양송이 버섯(Agaricus bisporus)의 에탄올 추출물의 항산화 효과와 Ames test를 통한 돌연변이 유발 억제능을 탐색하였다. 지질과산화물에 대한 단백질 보호효과를 알아본 결과, 양송이 버섯 에탄올 추출물은 MDA와 BSA의 교차결합 형성을 100mg/assay이었을 때 72.21% 저해하였다. $Fe^{2+}$에 의해 유도된 지질의 과산화에 대한 저해율은 2.0 mg/assay일 때 24.96% 였으며, DPPH 라디칼 소거 활성을 측정한 결과 1.0 mg/assay일 때 52.75%의 저해율을 보였으며 처리농도가 증가할수록 저해율은 증가하였다. $IC_{50}$ 값으로 항산화능의 상대적 비교를 하였을 때 양송이버섯 추출물은 DPPH 라디칼 소거 활성이 가장 강하였으며, 그 다음은 지질과산화 억제능이었고 MDA와 BSA의 교차결합 억제능이 가장 낮았다. Ames test를 이용하여 돌연변이 유발 억제능을 알아본 결과, 양송이버섯 에탄올 추출물은 Salmonella Typhimurium TA98 및 TA100 두 균주에서 직접작용 돌연변이능을 각각 100% 저해하여 매우 효과적이었다. 간접작용 돌연변이능 저해효과는 Salmonella Typhimurium TA98에서 86.09%, Salmonella Typhimurium TA100에서는 81.93%로 나타나 양송이버섯 에탄올 추출물은 직접, 간접작용 돌연변이능에 대한 저해효과가 우수하다고 사료된다. 본 연구 결과를 종합해 볼 때, 양송이버섯은 항산화성과 항돌연변이성이 우수하므로 기능성식품으로 개발할 수 있는 가능성이 있다고 본다.

• 한국식품조리과학회지
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• 제21권6호통권90호
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• pp.759-768
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• 2005

This study was performed to determine the antioxidative effect of the hexane, dichloromethane, ethylacetate, butanol and water fractions of Ganoderma lucidum extracts on the inhibition of malondialdehyde(MDA) and bovine serum albumin(BSA) conjugation reaction, the inhibition of lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl(DPPH) radical, the antimutagenic capacity as measured by the Ames test and the inhibitory effect on cancer cell. Ganoderma lucidum is believed to have possible antioxidative capacities, although the results have varied according to the assay method. The most effective antioxidative capacity was inhibition of lipid peroxidation. Among the five fractions, water fraction showed strong inhibition rates on MDA & BSA conjugation reaction, and ethylacetate fractions showed the most effective inhibition rate on lipid peroxidation and scavenging effect on DPPH radical. The indirect and direct antimutagenic effects of ethanol extracts of Ganoderma lucidum were examined by Ames test using Salmonella typhimurium TA98 and TA100. Among the samples, the water fraction did not have any antimutagenic effect. The inhibition rates on mutagenicity in the presence of 2.5 mg/plate were nearly $100\%$ for Salmonella typhimurium TA98 and TA100 except the hexane fraction of the direct mutagenicity mediated by 2-Nitrofluorene in Salmonella typimurium TA98($64.69\%$). Under the 2.5 mg/plate concentration, the inhibitory effects of hexane and dichloromethane fraction were superior to that of the other fractions on the direct mutagenicity for Salmonella typhimurium TA100 and indirect mutagenicity for Salmonella typhimurium TA98 and TA100. The inhibitory effect of Ganoderma lucidum extracts on cell proliferation in HeLa and MCF-7 was investigated by U test. The dichloromethane fraction showed highly antiproliferative effect in HeLa and MCF-7($IC_{50}$: 0.122 mg/mL, 0.272 mg/mL, respectively) cells while the water faction had a weak inhibitory effect($IC_{50}$: 0.691 mg/mL, 10.919 mg/mL respectively). These results suggest that Ganoderma lucidum may have antioxidative, antimutagenic and anticancer capacities and may be a candidate of the prevention and dietetic treatment of chronic diseases and the development of antioxidative, antimutagenic and anticancer functional food.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.247-252
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• 2013

An experiment was conducted to determine the effect of supplementing various concentrations (0, 100, 200, 300, or 400 ${\mu}g/kg$ diet) of organic Se on growth performance, carcass traits, oxidative stress, and immune responses in commercial broiler chickens reared in open-sided poultry house under tropical climatic conditions. Each diet was fed ad libitum to eight replicates consisting of six birds in each pen from 1 to 42 d of age. Body weight gain and feed efficiency, and relative weight of liver, abdominal fat and ready to cook yields were not affected (p>0.05) by organic Se supplementation to broiler diets. Lipid peroxidation in plasma decreased, while activities of glutathione peroxidase and glutathione reductase in plasma increased (p<0.01) linearly with Se concentration in diet. The ratios between heterophyls and lymphocytes and relative weight of lymphoid organs (bursa, spleen, and thymus), and antibody production to Newcastle disease vaccination were not affected (p>0.05) by Se supplementation to broiler diets. However, the cell-mediated immunity (lymphocyte proliferation ratio) increased (p<0.01) linearly with dietary Se concentration. The results of the present study indicate that the supplementation of Se did not influence body weight and feed efficiency. However, supplementation of Se increased antioxidant status and lymphocyte proliferation in broiler chickens.

• 한국식품영양과학회지
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• 제17권4호
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• pp.376-383
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• 1988

본 실험에서는 항산화제로 보고된 vitamin E와 Se이 부족된 상태에서 과산화반응의 원인물질이 되는 고도불포화지방산을 섭취시켰을때 여러수준의 methionine 이 생체내 지질과산화 반응에 미치는 영향을 알아보기 위하여 85g 내외의 Spraque-Dawley종 숫쥐 40마리를 5군으로 나누어 12주간 사육한 후 지질과산화물 함량, glutathione peroxidase활성도 및 전자현미경학적 조사를 하였으며, 그 결과 체중증가, 사료효율은 대조군과 비교했을때 MF군이 유의적으로 낮았고, 간의 중량 비대현상이 나타났다. 그란 0.3%-0.6%수준의 methionine공급에 의해서 호전될 수 있었다. 혈장과 간mitochondria 분획에서의 지질과산화물 함량은 대조군과 비교했을대 MF군이 유의적으로 높았는데, 0.6% methionine 공급에 의해서 저하시킬 수 있었다. 적혈구와 간mitochondria 분획에서의 GSH-Px 활성도는 MF준이 대조군에 비해서 유의적으로 낮았으나 3M군과 6M군에 비해서 높은 경향을 나타내었다. 간세포의 전자현미경조사에서는 MF군이 정상군에 비해서 지방소적, mitochondria의 swelling 및 융합, 그리고 disse강내 microvilli의 파괴가 관찰되었으나 methionine의 공급에 의해 다소 호전되었다. 그러나 정상적인 간세포 형태를 나타내지는 못하였다. 본 연구결과는 vitamin E와 Se의 부족 및 고도 불포화지방산을 섭취시켰을때 지질과산화물형성이 증가되며, 조직학적 변화를 유발하였다. 그러나 methionine에 의해서 이들 다소 억제시킬 수가 있었으며, 본 실험에서는 0.6%수준에서 비교적 양호하게 나타났다. 또한 methionine 단독으로서의 항산화력 증진 보다는 항산화제로서 관련된 영양소와의 상호작용에 의해 그 효과가 더욱 더 증대 될 수 있다고 활 수 있으며, 따라서 보다 균형적인 영양소 섭취에 의해서 지질과 산화의 유해한 반응으로부터 보호작용을 나타낼 수 있을 것으로 사료된다.